WEED SCIENCE
·
2025
·
IF 2.5
Wei Hu,Yuguo Li,Chaowei Zhao,Liya Zhang,Yumeng Ma,Quan Gao,Haiqun Cao,Min Liao
Barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] is a dominant weed species occurring in rice fields across China. Metamifop, a common herbicide, is frequently applied to control E. crus-galli and other grassy weeds in rice fields. Herein, HS01, an E. crus-galli population suspected to be resistant (R) to metamifop, was collected from Hanshan County in Anhui Province. Whole-plant dose-response testing revealed that, compared with the susceptible (S) population FD03, HS01 had developed high-level resistance to metamifop with a resistance index (RI) of 11.76 and showed cross-resistance to cyhalofop-butyl (RI=9.33), fenoxaprop-P-ethyl (RI=5.80) and clethodim (RI=3.24). Gene sequencing revealed a Cys-2088-Arg mutation in ACCase 1,5 allele of all the R plants, while ACCase gene overexpression was not involved in the resistance. Molecular docking indicated that the less negative binding energies might be the main reason for the resistance of HS01 to ACCase-inhibiting herbicides. A derived cleaved amplified polymorphic sequence (dCAPS) method was developed for the rapid identification of the Cys-to-Arg mutation in the ACCase gene at codon position 2088 in E. crus-galli. Additionally, pretreatment with the cytochrome P450 inhibitor piperonyl butoxide or the glutathione S-transferase inhibitor 4-chloro-7-nitrobenzoxa-diazole had no significant effects (p> 0.05) on the resistance of HS01 to metamifop. To our knowledge, this is the first report of a Cys-2088-Arg mutation in E. crus-galli ACCase that confers cross-resistance to ACCase-inhibiting herbicides.
研究领域:
植物学
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分子生物学
·
农业科学
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产品关联
DOI
高影响因子
Insect Science
·
2024
·
IF 2.9
Yongjie Zhang,Shulin He,Chengyin He,Ling Zhou,Ou Xu,Liang Qiao,Bin Chen,Yueqing Cao,Zhengbo He
The use of insecticides, primarily pyrethroids, is a pivotal strategy for mosquito control globally. Bioallethrin, the first commercially available volatile pyrethroid, can elicit spatial (i.e., noncontact) repellency to mosquitoes through the coactivation of olfactory receptor neurons and sodium channels. However, the olfactory mechanism of the repellency elicited by bioallethrin in mosquitoes is still unclear. Here, we demonstrated the involvement of AsOBP1 in the bioallethrin repellency in Anopheles sinensis , one of the main vectors of vivax malaria in China and other Southeast Asian countries. The behavioral and electrophysiological analyses in AsOrco −/− mutant found that the spatial repellency elicited by bioallethrin depended on the odorant receptor (OR)-mediated olfactory pathway. Furthermore, the repellency was reduced in the AsOBP1 −/− mutant and a pyrethroid-resistant strain, in which the expression of AsOBP1 was significantly decreased. Moreover, recombinant AsOBP1 protein bound to bioallethrin in an in vitro competition assay. These results indicate that activation of the AsOBP1 -mediated olfactory pathway is an important component of bioallethrin repellency. Our research lays the foundation for further elucidation into the olfactory mechanism of bioallethrin repellency and the behavioral modifications of pyrethroid-resistant mosquitoes.
研究领域:
昆虫学
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分子生物学
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公共卫生
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2019
·
IF 5.108
Yiping Tai,Nora Fung-Yee Tam,Weifeng Ruan,Yufen Yang,Yang Yang,Ran Tao,Jingfan Zhang
Wetland plants are proven to perform well in water treatment . However, the phytoremediation capability of wetland plants for antibiotics, especially the uptake and metabolism involved in vivo, is poorly understood. In this study, we investigated the removal, uptake, and specific metabolism by Canna indica and Iris pseudacorus of five sulfonamides (SAs) using hydroponic experiments for seven days. The removal of SAs ranged from 15.2% to 98.4% in the planted groups, whereas that in the unplanted control group was much lower (12.6%–39.9%). The accumulation of SAs in plants was in a concentration-dependent manner via an active process and is not a major removal mechanism (constituted 0.31%–3.62% of the total removal load in plant system). The results also showed differences in the removal and accumulation by plant species of SAs. The acetyl conjugates (N-acetyl SA) were formed, which significantly enhanced the uptake of SAs ( P < 0.001) except sulfapyridine. The concentrations of N-acetyl SA accounted for only 0.4%–23.8% of the total SAs distribution in plants, suggesting the involvement of other metabolism pathways. Methylation and oxidation metabolites were identified in plant tissues and no SA-induced growth stress occurred, revealing that antibiotic metabolism in vivo should be associated with the ability of wetland plants to accumulate antibiotic and tolerate antibiotic stress.
研究领域:
环境科学
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植物学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2025
·
IF 11.3
Yidong Zhou,Zhijian Ke,Jiazhuo Zhang,Wenbin Chang,Yarong Cui,Yimiao Chen,Yirui Huang,Chunli Xia,Wenjia Zhong,Zifa Deng,Dayong Jin
Microorganisms are constantly evolving to resist the toxicity of artificial antibiotics and preservatives released into the environment. Parabens, a group of alkyl and aryl esters of p -hydroxybenzoate, are a class of widely used antimicrobial preservatives, particularly effective against Gram-positive bacteria and fungi. Previous studies have reported the degradation of parabens by numerous Gram-negative strains and activated sludge, with degradation processes often involving transesterification. However, the physiological significance of transesterification has received little attention. Here, a Gram-positive bacterium, Microbacterium sp. S1037 was isolated and characterized for its ability to degrade eight parabens and completely mineralize them through p -hydroxybenzoate and protocatechuate ( β -ketoadipate pathway). Moreover, strain S1037 also possesses the ability of paraben-transesterification. Alcohols compete with water molecules for the active center of enzymes, accelerating the transesterification of parabens and inhibiting hydrolysis. Considering that the fate of the transesterification product is still hydrolysis, we speculated and proved that such a “superfluous” mechanism is a detoxification strategy, and could be used in the bioremediation of parabens in wastewater. Microbial transesterification of parabens has been reported worldwide, and we speculated that it might be a common detoxification choice in the evolution of microorganisms worldwide.
研究领域:
微生物学
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环境科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2019
·
IF 3.973
Yahui Zhang,Yi Yuan,Wei Chen,Jie Fan,Hong Lv,Qingsheng Wu
The abuse of antibiotics has led to widespread antimicrobial resistance (AMR) and environmental pollution. In order to solve these problems, a lot of studies have been carried out mainly focusing on the modification and recombination of organic reagents, but bacteria are still easy to adapt to it, so they cannot be thoroughly solved. Here, we present an integrated pollution-free synergistic antibacterial nanotechnology using inorganic nano-Cu 2 O, which could not only enhance the efficacy of aminoglycoside antibiotics, but also eliminate their environmental pollution by photocatalytic degradation. It was found that Cu 2 O showed significantly synergistic antibacterial effect (1+1>2) when combined with aminoglycoside antibiotics against Escherichia coli . The inhibition zone area increased by 59.0% when Cu 2 O combined with neomycin. This reduces dosage and the risk of AMR, and does not pollute the environment after degradation. Next, to explore the synergistic mechanisms, we have studied the interaction of antibiotics with nanoparticles, as well as the interaction of antibacterial agents with bacteria. At last, we believe that the destruction of cell walls by Cu 2 O facilitates the entry of antibiotics into cells is the reason for their synergy.
研究领域:
微生物学
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环境科学
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纳米技术
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药理学
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产品关联
DOI
PubMed
高影响因子
Advanced Materials Technologies
·
2022
·Vol. 8
·期 5
·2201195
·
IF 8.856
Jiwei jiang,Yuansong Wei,Zhaomin Chen,Jia Xu,Hui Zhang,Bin Dong
Pathogenic bacteria infection, which is an extremely serious clinical problem, could be effectively treated by the antibiotics, but the misuse of antibiotics caused by imprecise transportation is a huge problem that causes the emergence of multidrug-resistant bacteria, which can kill millions of people. Herein, a drug-carrier is developed that can deliver antibiotics precisely to the bacteria and kill bacteria efficiently, thus avoiding the misuse of antibiotics. These drug carriers, consisting of ciprofloxacin (cip)-loaded CaCO 3 coated by asymmetrical glycol chitosan (GCS) (cip-loaded CaCO 3 @GCS carriers, abbreviated as CC@GCs), could move autonomously in the acidic environment caused by the bacteria-infection, which is based on the asymmetric pressure generated by the CO 2 concentration gradient due to the asymmetric reaction between CaCO 3 and acid. The motion of CC@GCs increases its probability to contact the bacteria, leading to the formation of effective electrostatic binding between them. The combination of movement and enhanced affinity to bacteria enable CC@GCs to precisely deliver drug to bacteria and kill them more efficiently as compared to the free drug, which shows effective treatment in bacterial infection abscess on skin with improved drug utilization. The CC@GCs based drug carriers have great potentials in treating bacterial infection with precision for efficient antibacterial therapy and alleviating the antibiotic misusage.
研究领域:
生物医学工程
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微生物学
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药理学
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产品关联
DOI
高影响因子
JOURNAL OF PEST SCIENCE
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2025
·
IF 4.1
Lin Kai,Wu Hongxin,Li Zhongsheng,Zhong Zichun,He Liuyan,Guo Yujing,Zhang Jie,Xu Xiaoxia,Zhang Wenqing,Jin Fengliang,Pang Rui
Insecticide resistance in pest control poses a threat to agricultural production and human health. Numerous insect species express genes coding for detoxification enzymes that have broad substrate promiscuity thus conferring resistance to various insecticides. However, whether the homologs of these genes play similar roles in resistance phenotypes of closely related species remains largely unclear. Therefore, this study compares the resistance profiles of three major rice planthopper species (Delphacidae) ( Laodelphax striatellus , Nilaparvata lugens , and Sogatella furcifera ) based on the metabolic activity of their cytochrome P450s. Genome-wide analyses resulted in 68, 70, and 64 P450 genes in L. striatellus , N. lugens , and S. furcifera , respectively. Phylogenetic analyses among these genes found that most resistance-related genes in one species had homologs in other planthopper species. The most resistance-relevant orthogroup ( CYP6ER s) showed higher evolutionary instability than most other groups. RNAi and in vitro metabolism assays revealed that CYP6ER s confers more divergent insecticide resistance profiles among planthopper species than the other two major resistance-related P450 subfamilies ( CYP6AY s and CYP4C61 s). Alphafold-based structural predictions and alignments suggested that P450 orthogroups with higher phylogenetic instability tended to have less structural similarities, resulting in more divergent metabolic profiles. This relationship was also in silico validated on Aphidae aphids and Lepidoptera noctuids. This study proposes combined phylogenetic and toxicogenomic analyses for understanding CYPome-based insecticide resistance convergency and divergency among closely related pests. These findings may improve the accuracy and rationality of chemical pest control.
研究领域:
昆虫学
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毒理学
·
基因组学
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产品关联
DOI
高影响因子
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
·
2022
·Vol. 66
·期 5
·
IF 5.938
Yawei Du,Carlo Giannangelo,Wei He,Gerald J. Shami,Wenya Zhou,Tuo Yang,Darren J. Creek,Con Dogovski,Xinsong Li,Leann Tilley
Current best practice for the treatment of malaria relies on short half-life artemisinins that are failing against emerging Kelch 13 mutant parasite strains. Here, we introduce a liposome-like self-assembly of a dimeric artesunate glycerophosphocholine conjugate (dAPC-S) as an amphiphilic prodrug for the short-lived antimalarial drug, dihydroartemisinin (DHA), with enhanced killing of Kelch 13 mutant artemisinin-resistant parasites. Cryo-electron microscopy (cryoEM) images and the dynamic light scattering (DLS) technique show that dAPC-S typically exhibits a multilamellar liposomal structure with a size distribution similar to that of the liposomes generated using thin-film dispersion (dAPC-L). Liquid chromatography-mass spectrometry (LCMS) was used to monitor the release of DHA. Sustainable release of DHA from dAPC-S and dAPC-L assemblies increased the effective dose and thus efficacy against Kelch 13 mutant artemisinin-resistant parasites in an in vitro assay. To better understand the enhanced killing effect, we investigated processes for deactivation of both the assemblies and DHA, including the roles of serum components and trace levels of iron. Analysis of parasite proteostasis pathways revealed that dAPC assemblies exert their activity via the same mechanism as DHA. We conclude that this easily prepared multilamellar liposome-like dAPC-S with long-acting efficacy shows potential for the treatment of severe and artemisinin-resistant malaria.
研究领域:
药理学
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寄生虫学
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生物化学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Huixi Zhang,Shanxue Jiang,Haishu Sun,Yushuang Li,Zhiliang Yao
The emergence of antibiotic-resistant bacteria poses a severe threat to food safety and human health, necessitating an urgent search for novel antimicrobial agents that can be applied in the food industry. This study utilizes a deep learning approach to establish the optimal models for antibacterial activity against foodborne pathogens, particularly Escherichia coli and Staphylococcus aureus, as well as for predicting carcinogenicity. These optimal models are applied to screen natural products from the COCONUT database, resulting in the identification of 130 compounds with both antibacterial activity and noncarcinogenic properties. Two natural products, bis(hexamethylene)triamine and N-phenethylbiguanide, are selected for experimental validation of their antibacterial activity. The confirmation of antimicrobial properties validates the reliability of the models developed in this study. By providing an innovative approach for identifying antimicrobial agents for foodborne pathogens, this research offers new insights for discovering effective antimicrobials in an efficient manner.
研究领域:
食品科学
·
微生物学
·
人工智能
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 30
·14410-14420
·
IF 3.925
Yufei Zhang,Yijie Cheng,Yunjian Yu,Jie Li,Yuqing Hu,Yingchao Gao,Siyuan Huang,Wenbo Wang,Xinge Zhang
The emergence and rapid spread of bacterial resistance pose an extremely serious threat to treating infections. Inspired by the important role the spiny surface structure of a virus plays in mediating virus invasion, a spiny nanoplatform was designed and developed to eliminate drug-resistant pathogens. This biomimetic spiky surface structure increased not only the capture and internalization of bacteria but also the efficiency of light-to-heat conversion (∼36.27%), which facilitates bacterial elimination with an efficiency of around 100%. Notably, the spiny structure and surface chemical modification of the nanoplatform have a synergistic effect on the capture and phagocytosis of bacteria, which protect host cells from bacteria-triggered damage. The biomimetic nanoplatform possesses excellent biocompatibility and could also effectively disperse biofilm. Overall, the designed virus-like nanoplatform provides a reliable tool for the development of a new generation of biomimetic antibacterial agents and shows excellent prospects in fighting bacterial infection.
研究领域:
微生物学
·
纳米医学
·
感染性疾病
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 20
·12486
·
IF 6.208
Joanna Xuan Hui Goh,Loh Teng-Hern Tan,Jodi Woan-Fei Law,Kooi-Yeong Khaw,Nurul-Syakima Ab Mutalib,Ya-Wen He,Bey-Hing Goh,Kok-Gan Chan,Learn-Han Lee,Vengadesh Letchumanan
The increasing prevalence of resistance in carbapenems is an escalating concern as carbapenems are reserved as last-line antibiotics. Although indiscriminate antibiotic usage is considered the primary cause for resistance development, increasing evidence revealed that inconsequential strains without any direct clinical relevance to carbapenem usage are harboring carbapenemase genes. This phenomenon indirectly implies that environmental microbial populations could be the ‘hidden vectors’ propelling carbapenem resistance. This work aims to explore the carbapenem-resistance profile ofVibriospecies across diverse settings. This review then proceeds to identify the different factors contributing to the dissemination of the resistance traits and defines the transmission pathways of carbapenem resistance. Deciphering the mechanisms for carbapenem resistance acquisition could help design better prevention strategies to curb the progression of antimicrobial resistance development. To better understand this vast reservoir selecting for carbapenem resistance in non-clinical settings,Vibriospecies is also prospected as one of the potential indicator strains for carbapenem resistance in the environment.
研究领域:
微生物学
·
传染病学
·
抗生素研究
·
分子生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2025
·
IF 6.8
Chu Wang,Jingyao Guo,Jiaming Zhang,Chenxi Hou,Yunmo Xue,Xiaofeng Han,Yan Chen,Mingxin Wang,Qian Ma,Xiaolin Lu
The escalating threat of multidrug-resistant pathogens necessitates efficient antimicrobial development, in which antimicrobial peptides (AMPs) have been extensively studied due to their broad-spectrum antibiotic activity. Combining sum frequency generation and molecular dynamics simulation, we rationally designed AMPs by identifying two structural principles. Simply increasing the number of basic amino acids does not reliably improve antimicrobial efficacy; terminal (N’ and C’) phenylalanine residues can enhance AMP’s membrane interfacial activity via the hydrophobic effect. After two rounds of sequence optimization, among the derivatives we designed, an artificial AMP named GF demonstrated superior membrane binding (especially insertion) and spatial conformation stability. In vitro and in vivo evaluations revealed GF’s potential broad-spectrum efficacy against common bacteria and drug-resistant bacteria. Notably, GF exhibited enhanced antimicrobial potency over conventional antibiotics at lower concentrations. Our study established a bottom-up (mechanism-driven) design framework and provided a template for developing precision antimicrobials against resistant infections.
研究领域:
生物化学
·
微生物学
·
药物开发
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）