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Skin microbiome reconstruction and lipid metabolism profile alteration reveal the treatment mechanism of Cryptotanshinone in the acne rat

Zhaoming Zhu,Quanfu Zeng,Zhuxian Wang,Yaqi Xue,Tingting Chen,Yi Hu,Yuan Wang,Yufan Wu,Qun Shen,Cuiping Jiang,Chunyan Shen,Li Liu,Hongxia Zhu,Qiang Liu

Background Acne has become one of the most prevalent skin disorders , affecting mostly young people's physical and mental health globally. Cryptotanshinone (CPT) is a potential drug for acne, but its mechanism of acne treatment has not been thoroughly studied on the microbiota . Till date, only a few studies are directed to the impact of acne therapy on skin microbiota and lipid metabolites. Purpose The action mechanism of CPT treatment of acne was investigated by the strategy of microbiome integration with lipidomics . Methods The 16Sr DNA sequencing was used to detect skin microbiota composition, and absolute quantitative lipidomics was utilized to identify lipid metabolites profiles levels. Four key proteins of the glycolysis pathway were detected with the immunochemistry method. Antibacterial analysis was used to evaluate CPT treatment of acne. Results CPT significantly inhibited Staphylococcus epidermidis and Staphylococcus aureus . Combination of the skin microbiome and lipidomics analysis, 29 types of differentially expressed flora (DEFs) and 782 differentially expressed lipid metabolites (DELMs) were significantly altered, especially Staphylococcus, Corynebacterium , Ralstonia , Enhydrobacter, Burkholderia , and Streptococcus . Cer was mainly regulated by Staphylococcus and Corynebacterium , whereas TG and DG were mainly regulated by Ralstonia, Enhydrobacter, Burkholderia , and Streptococcus . The glycolysis pathway was significantly regulated by Staphylococcus on CPT treatment of acne. The energy metabolism, lipid metabolism , immune system, glycan biosynthesis , and metabolism could be reversed by CPT. Conclusion CPT might help acne rats rebuild their skin microbiota and alter lipid metabolism signatures. Furthermore, since skin microbes and skin lipid metabolites have a close correlation and are both regulated by CPT, the findings potentially provide a research foundation for the discovery of biomarkers of skin microbiome imbalance and targeted treatment of acne development mechanisms.