Membranes
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2022
·Vol. 12
·期 2
·239
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IF 4.562
Xiaolong Sun,Longfei Yin,Huayue Zhu,Junhao Zhu,Jiahuan Hu,Xi Luo,He Huang,Yongqian Fu
In this study, chitosan and sugarcane cellulose were used as film-forming materials, while the inorganic agent zinc oxide (ZnO) and natural compound phenyllactic acid (PA) were used as the main bacteriostatic components to fabricate biodegradable antimicrobial composite membranes. The water absorption and antimicrobial properties were investigated by adjusting the concentration of PA. The scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) results demonstrated that the components of the composite membrane were successfully integrated. The addition of ZnO improved the mechanical and antimicrobial properties of the composite membrane, while the addition of PA with high crystallinity significantly reduced the water absorption and swelling. Moreover, the addition of 0.5% PA greatly improved the water absorption of the composite membrane. The results of antimicrobial experiments showed that PA improved the antimicrobial activity of the composite membrane againstStaphylococcus aureus,Escherichia coli,Aspergillus nigerandPenicillium rubens. Among them, 0.3% PA had the best antimicrobial effect againstS. aureus,E. coliandA. niger, while 0.7% PA had the best antimicrobial effect againstP. rubens.Keywords:composite membrane;phenyllactic acid;antimicrobial activity
研究领域:
材料科学
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生物学
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6
Fangquan Liu,Siyu Yang,Lei Zhang,Meiyue Zhang,Ying Bi,Shuo Wang,Xuekun Wang,Yinhu Wang
Antimicrobial resistance has emerged as a significant threat to global public health. To develop novel, high efficiency antibacterial alternatives to combat multidrug-resistant bacteria, A total of thirty-two novel amphiphilic benzopyran derivatives by mimicking the structure and function of antimicrobial peptides were designed and synthesized. Among them, the most promising compounds 4h and 17e displayed excellent antibacterial activity against Gram-positive bacteria (MICs = 1–4 μg/mL) with weak hemolytic activity and good membrane selectivity. Additionally, compounds 4h and 17e had rapid bactericidal properties, low resistance frequency, good plasma stability, and strong capabilities of inhibiting and eliminating bacterial biofilms. Mechanistic studies revealed that compounds 4h and 17e could effectively disrupt the integrity of bacterial cell membranes, and accompanied by an increase in intracellular reactive oxygen species and the leakage of proteins and DNA, ultimately leading to bacterial death. Notably, compound 4h exhibited comparable in vivo antibacterial potency in a mouse septicemia model infected by Staphylococcus aureus ATCC43300, as compared to vancomycin. These findings indicated that 4h might be a promising antibacterial candidate to combat antimicrobial resistance.
研究领域:
微生物学
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药物化学
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感染病学
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DOI
PubMed
高影响因子
American Journal of Translational Research
·
2024
·Vol. 16
·期 5
·2011
·
IF 1.7
Ling Cao,Lili Tan,Liping Li
Objectives: The increasing issue of bacterial resistance, coupled with inadequate progress in developing new antibiotics, necessitates exploring alternative treatments. Antibacterial biomaterials, such as silver and copper, possess advantageous properties such as heat resistance, durability, continuity, and safety. Particularly, they can effectively eliminate pathogenic bacteria while preserving cellular integrity, emphasizing the necessity of identifying optimal metal ion concentrations for practical application. Caenorhabditis elegans ( C. elegans ) can serve as a noteworthy model in this context. This study employed a C. elegans infection model to assess the efficacy of antibacterial metal ions. Methods: Hematoxylin-eosin (HE) staining and inductively coupled plasma mass spectrometry (ICP-MS) assay were utilized to determine the toxic levels of metal ions in mice. Additionally, RNA sequencing (RNA-seq) and assessment of reactive oxygen species (ROS) production in the C. elegans model were conducted to elucidate the mechanisms underlying metal ion toxicity. Results: Silver ion concentrations ranging from 10 -6 to 10 -7 M and copper ion concentrations ranging from 10 -4 to 10 -5 M exhibited antimicrobial properties without eliciting cytotoxic effects. Analysis of the transcriptome data derived from mRNA isolated from C. elegans indicated that CRKP infection activated the FoxO signaling pathway, potentially leading to ROS accumulation and C. elegans demise. Conclusions: In conclusion, C. elegans serves as a comprehensive infection model for assessing antibacterial metal ions.
研究领域:
微生物学
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分子生物学
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药理学
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DOI
PubMed
高影响因子
Micromachines
·
2021
·Vol. 12
·期 9
·1116
·
IF 2.891
Shuiqin Chai,Lijia Zhou,Shuchen Pei,Zhiyuan Zhu,Bin Chen
It is a major challenge to effectively inhibit microbial pathogens in the treatment of infectious diseases. Research on the application of nanomaterials as antibacterial agents has evidenced their great potential for the remedy of infectious disease. Among these nanomaterials, carbon quantum dots (CQDs) have attracted much attention owing to their unique optical properties and high biosafety. In this work, P-doped CQDs were prepared by simple hydrothermal treatment of m-aminophenol and phosphoric acid with fluorescence emission at 501 nm when excited at 429 nm. The P-doped CQDs showed effective antibacterial activity againstEscherichia coli(E. coli) andStaphylococcus aureus(S. aureus). The minimal inhibitory concentrations (MICs) of P-doped CQD were 1.23 mg/mL forE. coliand 1.44 mg/mL forS. aureus. Furthermore, the morphologies ofE. colicells were damaged andS. aureusbecame irregular when treated with the P-doped CQDs. The results of zeta potential analysis demonstrated that the P-doped CQDs inhibit antibacterial activity and destroy the structure of bacteria by electronic interaction. In combination, the results of this study indicate that the as-prepared P-doped CQDs can be a promising candidate for the treatment of bacterial infections.Keywords:carbon quantum dots;biocompatibility;antibacterial activity
研究领域:
纳米材料学
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微生物学
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医学
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DOI
PubMed
高影响因子
Waste and Biomass Valorization
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2025
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IF 2.8
Zhou Caixue,Dai Shuxia,Yin Li,Zhuo Yu,Zhao Nannan,Li Dehai,Sun Changyan
Purpose In this paper, we use Pinus pumila (Pall.) Pinecone to extract essential oil and improve its utilization. Methods The study used hydrodistillation to extract essential oil from Pinus pumila (Pall.) pinecone. Inhibition analysis of E. coli using extracted essential oils was evaluated to reveal the inhibitory activity by assessing growth curves, conductivity, nucleic acids, proteins, enzyme activities, and protein synthesis. Results The primary constituents, as shown by GC-MS chemical composition study, were α-pinitol, β-pinene and limonene. The results of the bacteriostatic activity indicated that the inhibitory zone (DIZ) had a diameter of 14.93 ± 0.14 mm, the minimum bactericidal concentration (MBC) was 16.0 µL/mL, and the minimum inhibitory concentration (MIC) was 8.0 µL/mL. Sweeping electron microscopy and laser confocal also demonstrated the disruption of cell wall and cellular enzymes, analyzed by CATase and ATPase activities, which showed a decrease in enzyme activity, and SDS-PAGE analysis showed its ability to inhibit protein synthesis, which leads to cellular damage. Conclusion The present study emphasizes the sustainable use of Pinus pumila (Pall.) Pinecone for economic opening of food preservation in addition to inhibition of spoilage bacteria.
研究领域:
植物学
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微生物学
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食品科学
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DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2021
·
IF 4.598
Junqing Qian,Yan Chen,Qian Wang,Xiaohua Zhao,Haiyan Yang,Feng Gong,Hui Guo
Nisin is food preservative with good food safety and is widely used around the world. However, nisin degrades during food processing and storage, seriously affecting its antibacterial function. In order to improve the stability of nisin, nisin microcapsules were prepared by complex embedding nisin with pectin and chitosan. By selecting the mass ratio of pectin to nisin and chitosan, the microcapsules of nisin loading 200 mg with 81.15% encapsulation efficiency were prepared in aqueous solution of pH 4.0. The results of zeta potential of pectin, chitosan, nisin and microcapsules, infrared spectra and X-Ray diffraction spectra confirmed the embedding of nisin by pectin-chitosan. The adsorption isotherm of pectin and chitosan showed that chitosan and pectin mainly adsorbed by monolayer to form microcapsules. Compared with the same amount of free nisin, bactericidal efficiency decreased within 24 h, which showed there is a release process in microcapsules. However, after treated with 121 °C for 10 min, the sterilization activity loss of nisin was significantly lower than that of free nisin, which showed the thermal stability of nisin pectin microcapsules was greatly increased. The nisin microcapsules with good thermal stability and sustained-release effect can be better applied in future.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
Polymers
·
2021
·Vol. 13
·期 16
·2812
·
IF 4.329
Meng Zhang,Dong Wang,Nana Ji,Shaoxiang Lee,Guohui Wang,Yuqi Zheng,Xin Zhang,Lin Yang,Zhiwei Qin,Yang Yang
Due to the spread of drug-resistant bacteria in hospitals, the development of antibacterial dressings has become a strategy to control wound infections caused by bacteria. Here, we reported a green strategy for in situ biomimetic syntheses of silver nanoparticles@organic frameworks/graphene oxide (Ag@MOF–GO) in sericin/chitosan/polyvinyl alcohol hydrogel. Ag@MOF–GO was synthesized in situ from the redox properties of tyrosine residues in silk sericin without additional chemicals, similar to a biomineralization process. The sericin/chitosan/Ag@MOF–GO dressing possessed a high porosity, good water retention, and a swelling ratio. The hemolysis rate of the composite was 3.9% and the cell viability rate was 131.2%, which indicated the hydrogel possessed good biocompatibility. The composite also showed excellent lasting antibacterial properties against drug-sensitive and drug-resistant pathogenic bacteria. The composite possessed excellent hemostatic activity. The coagulation effect of the composite may be related to its effect on the red blood cells and platelets, but it has nothing to do with the activation of coagulation factors. An in vitro cell migration assay confirmed and an in vivo evaluation of mice indicated that the composite could accelerate wound healing and re-epithelialization. In summary, the composite material is an ideal dressing for accelerating hemostasis, preventing bacterial infection, and promoting wound healing.Keywords:biomimetic synthesis;hemostasis;antibacterial activity;wound healing
研究领域:
生物材料学
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医学
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纳米生物学
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DOI
PubMed
高影响因子
Frontiers in Microbiology
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2021
·
IF 5.64
Fan Qiuxia,Liu Cheng,Gao Zhenpeng,Hu Zhongqiu,Wang Zhouli,Xiao Jianbo,Yuan Yahong,Yue Tianli
Alicyclobacillus acidoterrestris (A. acidoterrestris), a spore-forming bacterium, has become a main challenge and concern for the juices and acid beverage industry across the world due to its thermo-acidophilic characteristic. Thymoquinone (TQ) is one of the active components derived from Nigella sativa seeds. The objective of this study was to investigate antibacterial activity and associated molecular mechanism of TQ against A. acidoterrestris vegetative cells, and to evaluate effects of TQ on A. acidoterrestris spores and biofilms formed on polystyrene and stainless steel surfaces. Minimum inhibitory concentrations of TQ against five tested A. acidoterrestris strains ranged from 32 to 64 μg/mL. TQ could destroy bacterial cell morphology and membrane integrity in a concentration-dependent manner. Field-emission scanning electron microscopy observation showed that TQ caused abnormal morphology of spores and thus exerted a killing effect on spores. Moreover, TQ was effective in inactivating and removing A. acidoterrestris mature biofilms. These findings indicated that TQ is promising as a new alternative to control A. acidoterrestris and thereby reduce associated contamination and deterioration in the juice and acid beverage industry.
研究领域:
食品科学
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微生物学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 42
·19704-19713
·
IF 3.591
Yunan Zhang,Yu Duan,Jin Su,Lixin Liu,Yanru Feng,Lili Wu,Lei Zhang,Yunjie Zhang,Dongyu Zou,Yingli Liu
In order to improve the solubility of pipemidic acid (PPA) which belongs to quinolones and becomes an “old drug” due to its low aqueous solubility that restricts its application, two unreported pharmaceutical salts of PPA with catecholcarboxylic acid (CCA) (HPPA·C7H5O4·2(H2O)) and m-hydroxybenzoic acid (MHA) (HPPA·C7H5O3·C7H6O3·2(H2O)) have been designed, synthesized and characterized. A comprehensive analysis of the structure and Hirshfeld surface reveals that the CAHBs formed from the N atoms in the piperazine ring from PPA molecules with the carboxylic acid group in the coformers could have a stable crystal structure. As expected, the solubility experiments showed that the solubilities of the PPA–CCA and PPA–MHA were significantly improved compared with that of the PPA, which may be due to the increased hydration enthalpy resulting from the formation of CAHBs between PPA and coformers, which leads to increased solubility. Moreover, the antibacterial activities of PPA–CCA and PPA–MHA are also improved. At the same time, surprisingly, the permeability of PPA–CCA and PPA–MHA was also better than that of PPA. In addition, the hygroscopic stabilities of PPA–CCA and PPA–MHA were significantly improved compared with that of PPA. The reason may be due to that the hydrogen bonds between PPA and CCA weaken their attraction to external water molecules. Thus, the present work implies that CAHBs play a key role in adjusting the molecular packing via a cocrystallization strategy to improve the physicochemical properties of quinolones, which could offer a tremendous opportunity to recycle these “old” drugs and give them a new life.
研究领域:
药学
·
化学
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DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Yuanpeng Hao,Jiamu Kang,Rui Yang,Hui Li,Hongxia Cui,Hongtong Bai,Andrey Tsitsilin,Jingyi Li,Lei Shi
Numerous species of Origanum (Lamiaceae) have been widely used as spices to extend the shelf life of foods. Essential oils extracted from this genus have attracted much attention owing to their potential applications as bactericides. Here, we evaluated the chemical compositions of eight oregano essential oils (OEOs) using gas chromatography-mass spectrometry and assessed their antibacterial activities. The chemical compositions of OEOs were affected by the cultivar factor, and seven common compounds, including carvacrol, were identified among eight OEOs. Partial least squares discriminant analysis enabled the distinction of three groups among these OEOs, as characterized by the proportions of carvacrol, thymol , and sesquiterpenes. OEOs effectively inhibited Escherichia coli and Staphylococcus aureus with varying antibacterial activities. Spearman correlation network highlighted core antibacterial contributors in the chemical profiles of OEOs. Our results revealed that the bacteriostatic effects of OEOs could be explained by core compounds and their synergistic effects.
研究领域:
植物学
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微生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Bioengineering & Translational Medicine
·
2021
·Vol. 7
·期 2
·e10274
·
IF 10.711
Liming Zhou,Nanbo Liu,Longbao Feng,Mingyi Zhao,Peng Wu,Yunfei Chai,Jian Liu,Ping Zhu,Rui Guo
Bacterial infection is one of the most frequent complications in the burn and chronic wounds. Inspired by natural existing superhydrophobic surface structures, a novel asymmetric wettable membrane was prepared using the electrospinning technique for facilitating the bacteria-infected wound healing. Herein, the prepared membrane consists of two layers: The hydrophobic outer layer was composed of poly (lactic-co-glycolic) acid (PLGA) and black phosphorus-grafted chitosan (HACC-BP), while the hydrophilic inner layer was composed by using a mixture of gelatin (Gel) with ginsenoside Rg1 (Rg1). Biological studies in vitro showed BP@PLGA/Gel (BP@BM) membrane with excellent antibacterial activity could significantly inhibit the adhesion of bacteria, and Rg1 could facilitate the migration and tube formation of human umbilical vein endothelial cells (HUVECs). Compared to Aquacel Ag dressing, the result in vivo revealed that the Rg1/BP@BM could facilitate better wound healing by triggering phosphoinositide 3-kinase (P-PI3K/PI3K) and phosphorylation of protein kinase B (P-AKT/AKT) signaling pathways, upregulating Ki67, CD31, α-SMA, and TGF-β1, and downregulating TNF-α, IL-1β, and IL-6, promoting M2 polarization (IL-10, CD206, and Arg-1) of macrophages, inhibiting M1 polarization (iNOS) of macrophages. These findings suggested that the asymmetric wettable membrane have the huge potential for wound healing.
研究领域:
生物医学工程
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材料科学
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药理学
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产品关联
DOI
PubMed
高影响因子
Marine Drugs
·
2012
·Vol. 10
·期 12
·2634-2647
·
IF 3.854
Jiaojiao Zheng,Yicun Chen,Fen Yao,Weizhou Chen,Ganggang Shi
Gloiopeltis tenax(G. tenax) is widely distributed along the Chinese coastal areas and is commonly used in the treatment of diarrhea and colitis. This study aimed at investigating the bioactivities of the volatile constituents inG. tenax. We extracted the essential constituents ofG. tenaxby supercritical carbon dioxide extraction (CO2-SFE), then identified and analyzed the constituents by gas chromatography-mass spectrometry (GC-MS). In total, 30 components were identified in theG. tenaxextract. The components showed remarkable antioxidant activity (radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH)), lipid peroxidation inhibition capacity (in a β-carotene/linoleic acid-coupled oxidation reaction), and hydroxyl radical-scavenging activity (by deoxyribose degradation by iron-dependent hydroxyl radical), compared to butylated hydroxytoluene. In microdilution assays,G. tenaxextracts showed a moderate inhibitory effects onStaphyloccocus aureus(minimum inhibitory concentration (MIC) = 3.9 mg/mL),Enterococcus faecalis(7.8 mg/mL),Pseudomonas aeruginosa(15.6 mg/mL), andEscherichia coli(3.9 mg/mL). Antioxidant and antimicrobial activities ofG. tenaxwere related to the active chemical composition. These results suggest that the CO2-SFE extract fromG. tenaxhas potential to be used as a natural antioxidant and antimicrobial agent in food processing.Keywords:Gloiopeltis tenax;GC-MS;CO2-SFE;antioxidant activity;antimicrobial activity
研究领域:
海洋生物学
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食品科学
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药用植物学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）