Processes
·
2022
·Vol. 10
·期 1
·124
·
IF 3.352
Xiao Ma,Zhenjun Wang
Ciprofloxacin (CIP), as a common antibiotic used in human clinical and livestock farming, is discharged into natural water bodies and its concentration has increased in the last years. Its stable chemical structure is difficult to remove by conventional techniques. Residual ciprofloxacin in the environment has become an emerging micropollutant that promotes the generation of resistance genes of bacteria and endangers ecosystem balance and human health. Removal of ciprofloxacin from water by the system of ultrasound/electric field/sodium persulfate (US/E/PS) was investigated. Firstly, CIP degradation affects by different oxidation methods, such as ultrasonic oxidation, electro-oxidation, and persulfate oxidation, and their four combined oxidation methods (ultrasound-activated persulfate oxidation, electro-activated persulfate oxidation, ultrasound-enhanced electro-oxidation, and ultrasound-enhanced electro-activated persulfate oxidation), on the target contaminants were compared. Secondly, the influences of parameters on the CIP degradation by an ultrasound-enhanced electro-activation-persulfate reaction system were investigated. Thirdly, the possible free radical species in the ultrasound-enhanced electro-activation-sulfate reaction system were identified and the dominant free radical species in the system were analyzed. Finally, the samples of CIP in the US/E/PS system were tested by liquid mass spectrometry, and the possible intermediate products and degradation path were speculated. The results indicate that the US/E/PS system is of great potential application value in the removal of organic pollution and environmental purification.Keywords:ultrasound;antimicrobial drug;degradation;ciprofloxacin
研究领域:
环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
Antibiotics-Basel
·
2022
·Vol. 11
·期 6
·807
·
IF 5.222
Jing Cao,Yajie Wang,Guanzhao Wang,Pingping Ren,Yongning Wu,Qinghua He
Antimicrobials may be used to inhibit the growth of micro-organisms in the cultivation of mung bean sprouts, but the effects on mung bean sprouts are unclear. In the present study, the growth performance, morphology, antimicrobial effect and antimicrobial residues of mung bean sprouts cultivated in typical antimicrobial solutions were investigated. A screening of antimicrobial residues in thick-bud and rootless mung bean sprouts from local markets showed that the positive ratios of chloramphenicol, enrofloxacin, and furazolidone were 2.78%, 22.22%, and 13.89%, respectively. The cultivating experiment indicated that the production of mung bean sprouts in antimicrobial groups was significantly reduced over 96 h (p< 0.05). The bud and root length of mung bean sprouts in enrofloxacin, olaquindox, doxycycline and furazolidone groups were significantly shortened (p< 0.05), which cultivated thick-bud and rootless mung bean sprouts similar to the 6-benzyl-adenine group. Furthermore, linear regression analysis showed average optical density of 450 nm in circulating water and average production had no obvious correlation in mung bean sprouts (p> 0.05). Antimicrobial residues were found in both mung bean sprouts and circulating water. These novel findings reveal that the antimicrobials could cultivate thick-bud and rootless mung bean sprouts due to their toxicity. This study also proposed a new question regarding the abuse of antimicrobials in fast-growing vegetables, which could be a potential food safety issue.Keywords:Vigna radiata;mung bean sprouts;antimicrobials;production;morphology;residues
研究领域:
食品科学
·
植物生理学
✓ 已验证
DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2022
·Vol. 85
·期 12
·1017-1028
·
IF 2.213
Gao Yanlin,Chen Cen,Wang Ren,Li Zehua,Cheng Qiaoyuan
Currently, illegal addition of anti-infective drugs has been detected in some cosmetics to achieve rapid sterilization and anti-inflammatory effects. However, the abuse of anti-infective drugs may endanger the health of citizens. Thus, methods for the detection and quantitative determinations of such substances should be developed. In this paper, a method combining ultra-high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF HRMS) was designed. Its significance and potential were verified by the identification, confirmation, and quantification of 37 illegally added anti-infective drugs in cosmetics that claimed to have anti-acne effects. The method validation revealed a satisfactory linear relationship, and each coefficient of determination ( r 2 ) was higher than 0.9943. The LOD and LLOQ results were in the ranges of 0.0003–0.1063 μg g −1 and 0.0012–0.3545 μg g −1 , respectively. The accuracy ranged from 70.8 to 122.8%, whereas the intra- and inter-day precisions were in the ranges of 0.2–12.8% and 0.2–13.8%, correspondingly. The mean recoveries were in the range of 70.1–117.7%, and the matrix effects ranged from 73.0 to 121.4%. Of the 200 batches of cosmetics that claimed to have anti-acne effects, three batches were positive, with chemical components such as metronidazole, lincomycin, clindamycin, tinidazole and miconazole nitrate being discovered. Therefore, the Q-TOF spectrometry could be widely applied in routine screening of adulteration in cosmetics.
研究领域:
分析化学
·
药学
·
医学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xinwei Tao,Zijia Wang,Bowen Ren,Jianliang Li,Tianle Zhou,Huaping Tan,Xiaohong Niu
Flexible chitosan-based membranes were prepared by casting/solvent evaporation method using chitosan flocks as raw material. To improve mechanical and biological properties, chitosan microspheres (CMs) were prepared and integrated to form the composite membranes. Two different anti-bacterial drugs, e.g., tetracycline hydrochloride (TH) and silver sulfadiazine (AgSD), were loaded into the CMs and composite membranes to enhance their anti-bacterial properties. Furthermore, composite membranes were alternately coated by multi-layers of oxidized alginate (OAlg) and carboxymethyl chitosan (CMCS) via the layer-by-layer self-assembly and Schiff-base cross-linking. Our results demonstrated that the microspheres and multi-layer coatings could improve the swelling, water vapor transmission and hydrophilicity of the composite membranes. The chitosan microspheres and multi-layer coatings increased the tensile strength and decreased the elongation at the break of the membranes. Our composite membrane had better mechanical properties, slow drug release, anti-bacterial properties, which could promote cell proliferation. This composite membrane has great application potential in inhibiting bacterial infection and promoting wound regeneration.
研究领域:
生物材料学
·
药剂学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Ye Ma,Lian Xin,Huaping Tan,Ming Fan,Jianliang Li,Yang Jia,Zhonghua Ling,Yong Chen,Xiaohong Hu
Transparent and flexible chitosan-based membranes containing antibacterial drugs were prepared through a casting/solvent evaporation method from suspension of chitosan floccule. To enhance mechanical properties, glycerin was incorporated into the floccule suspension to conjugate chitosan as a plasticizer. The mechanism of membrane formation is attributed to inter- and intro-hydrogen bonding between chitosan and glycerol molecules. The results showed that incorporation of glycerol has a significant influence on the properties of the chitosan membranes. With the increase of glycerol content, the tensile strength, swelling rate, water vapor permeability and wettability of membranes were significantly improved. In vitro enzymatic degradation revealed that the chitosan membrane had long-term stability regardless of the glycerol content. To enhance antibacterial properties, tetracycline hydrochloride (TH) and silver sulfadiazine (AgSD), representing the water-soluble and water-insoluble drug models, were integrated into the membranes, respectively. The results of controlled-release efficacy and inhibition zone indicate that the glycerol toughened chitosan membranes containing drugs have a promising future in treatment of bacterial infection as wound dressing.
研究领域:
生物材料学
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
CELLULOSE
·
2020
·Vol. 27
·期 5
·2737-2756
·
IF 4.21
Shen Zhi,Cai Ning,Xue Yanan,Yu Bo,Wang Jianzhi,Song Hao,Deng Hang,Yu Faquan
Abstract A sustained release of anti-infection drugs is beneficial for the reduction of required dressing change times and the protection of the granulation tissue against further infection. In this work, the strategy of encapsulating mesoporous SBA-15 particles in a permeable cellulose membrane (CM) was used in the preparation of antibacterial cellulose based composite membranes to depot store and deliver drugs with prolonged anti-microbial drug release characteristics. It was found that SBA-15 with the protection of phosphate buffered saline prefilled in SBA-15 mesoporous channel could resist strong alkali environment during composite membranes preparation and its mesoporous structure remained intact, which was verified by SEM, TEM and BET results. The resultant cellulose based composite membrane containing 30 wt% SBA-15 (denoted as P-CM-SBA (30%)) had achieved 3.6 wt% drug loading and demonstrated the sustained release of chloramphenicol for 250 h, which was resulted from SBA-15/cellulose structure-inducing two-stage release behavior. Strong antibacterial activity of P-CM-SBA (30%) against S. aureus and E. coli . could last 144 h. In addition, the tensile strength, water vapor transmission rate and swelling properties of P-CM-SBA (30%) conformed to the primary requirements of wound dressing materials. Therefore, cellulose based composite membrane with 30 wt% SBA-15 particles possesses the potential for the application of wound healing. Graphic abstract
研究领域:
材料科学
·
生物医学工程
·
药剂学
✓ 已验证
DOI
高影响因子
Communications Biology
·
2025
·Vol. 8
·期 1
·1-14
·
IF 5.1
Xu Tianqi,Fang Dan,Li Fulei,Wang Zhiqiang,Liu Yuan
Antimicrobial resistance poses a severe threat to human health, with colistin serving as a critical medication in clinical trials against multidrug-resistant Gram-negative bacteria. However, the efficacy of colistin is increasingly compromised due to the rise of MCR-positive bacteria worldwide. Here, we reveal a notable metabolic disparity between mcr -positive and -negative bacteria through transcriptome and metabolomics analysis. Specifically, pyridoxal 5’-phosphate (PLP), the active form of vitamin B6, was significantly diminished in mcr -positive bacteria. Conversely, supplementing with PLP could reverse the metabolic profile of drug-resistant bacteria and effectively restore colistin’s bactericidal properties. Mechanistically, PLP was found to augment bacterial proton motive force by inhibiting the Kdp transport system, a bacterial K + transport ATPase, thereby facilitating the binding of the positively charged colistin to the negatively charged bacterial membrane components. Furthermore, PLP supplementation triggers ferroptosis-like death by accumulating ferrous ions and inducing lipid peroxidation. These two modes of action collectively resensitize mcr -harboring Gram-negative bacteria to colistin therapy. Altogether, our study provides a novel metabolic-driven antibiotic sensitization strategy to tackle antibiotic resistance and identifies a potentially safe antibiotic synergist.
研究领域:
微生物学
·
药理学
·
代谢组学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Huanhuan Shi,Zhengli Hou,Yali Zhao,Kaixuan Nie,Bo Dong,Lemeng Chao,Shenghong Shang,Mengqiu Long,Zhengchun Liu
A simple microfluidic concentration gradient generator (MCG) was developed to create concentration gradients on a centimeter scale with a wide range of flow rates. Four groups of inlets (sample and buffer) were successively connected to the main channel to determine the location of inlets and the type of laminar flow and to create the concentration gradients. Nine micromixing channels were derived from the main channel to efficiently mix laminar flow. A micromixing channel whose mixing index was higher than that of a conventional serpentine mixing channel was designed by combining a square wave structure and microchannel sidewall grooves. A spindle-shaped chamber was set at the end of the mixing channel to observe the gradients. The computational fluid dynamic software Fluent was used to predict concentrations by calculating the average mass fraction at the cross section of each outlet. Theoretical simulations indicated that linear concentration gradients would be achieved within seconds in accordance with the fast micromixing principle at a flow rate of 2280 μL/min, and the gradient shape was steady regardless of the flow rate, which was affected by the location of inlet ports. The uniformity of the mass fraction achieved by the improved mixing channel was better than that of conventional serpentine micromixing channel. The theoretical simulations were verified through the generation of a concentration gradient of methylene blue water solution. Multiple concentrations of an antibacterial drug (ceftizoxime) generated by this MCG were utilized for an antimicrobial susceptibility test using Escherichia coli . Results revealed that the MCG showed potential for concentration gradient-related biochemical study.
研究领域:
生物工程
·
微生物学
✓ 已验证
DOI
高影响因子
POLYMER TESTING
·
2019
·
IF 2.943
Lian Xing,Ye Ma,Huaping Tan,Guoliang Yuan,Shengke Li,Jianliang Li,Yang Jia,Tianle Zhou,Xiaohong Niu,Xiaohong Hu
Transparent and flexible alginate-based membranes containing antibacterial drugs were prepared by a casting/solvent evaporation method from alginate solution mixed with chitosan floccule (CSF) and oxidized sodium alginate (OAlg). To improve the properties of the membranes, CSF and OAlg were added to the alginate solution in different ratios. The mechanism of membrane formation is attributed to two aspects: Schiff-base cross-linking between CSF and OAlg; ion cross-linking between sodium alginate and CaCl 2 . The results showed that the incorporation of CSF and OAlg had a significant effect on the properties of the alginate membrane. With the change in the ratio between CSF and OAlg, the tensile strength , swelling rate, water vapor permeability, wettability and degradation stability of the membranes were significantly changed. In order to enhance the anti-bacterial properties, clindamycin (DA) and silver sulfadiazine (AgSD), which represent water-soluble and water-insoluble drug models, were integrated into the membrane, respectively. The results of controlled release effects and inhibition zones indicate that the alginate membranes containing drugs have a promising future in treatment of bacterial infection as wound dressing.
研究领域:
生物材料学
·
药剂学
·
医学工程
✓ 已验证
DOI
高影响因子
Science Advances
·
2024
·Vol. 10
·期 12
·
IF 11.7
Xianyuan Wei,Bin Xue,Shuangchen Ruan,Jintong Guo,Yujing Huang,Xiaorui Geng,Dan Wang,Cangtao Zhou,Jun Zheng,Zhen Yuan
To eliminate multidrug-resistant bacteria of Acinetobacter baumannii, we screened 1100 Food and Drug Administration–approved small molecule drugs and accessed the broxyquinoline (Bq) efficacy in combination with various metal ions. Antibacterial tests demonstrated that the prepared Zn(Bq)2 complex showed ultralow minimum inhibitory concentration of ~0.21 micrograms per milliliter with no resistance after 30 passages. We then constructed the nano zeolitic imidazolate framework-8 (ZIF-8) as a drug carrier of Zn(Bq)2 and also incorporated the photosensitizer chlorin e6 (Ce6) to trace and boost the antibacterial effect. To further ensure the stable and targeted delivery, we genetically engineered outer membrane vesicles (OMVs) with the ability to selectively target A. baumannii. By coating the ZnBq/Ce6@ZIF-8 core with these OMV, the resulted drug (ZnBq/Ce6@ZIF-8@OMV) exhibited exceptional killing efficacy (>99.9999999%) of A. baumannii. In addition, in vitro and in vivo tests were also respectively carried out to inspect the remarkable efficacy of this previously unknown nanodrug in eradicating A. baumannii infections, including biofilms and meningitis.
研究领域:
微生物学
·
药理学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 35
·22893-22901
·
IF 4.036
Nannan Chen,Lijuan Yang,Na Ding,Guiwen Li,Jiajing Cai,Xiaoli An,Zhijie Wang,Jie Qin,Yuzhen Niu
Metronidazole is a specific drug against trichomonas and anaerobic bacteria, and is widely used in the clinic. However, extensive clinical application is often accompanied by extensive side effects, so it is still of great significance to develop metronidazole derivatives with a new skeleton. Compared with other traditional receptor-based drug design methods, the computational model based on a neural network has higher accuracy and reliability. In this work, a Recurrent Neural Network (RNN) model is applied to the discovery of metronidazole drugs with a new skeleton. Firstly, the generation model based on a Gated Recurrent Unit (GRU) is trained to generate an effective Simplified Molecular-Input Line-Entry System (SMILES) string library with high precision. Then, transfer learning is introduced to fine-tune the GRU model, and many molecules with structures similar to known active drugs are generated. After cluster analysis of the structures of the new compounds, 20 small molecular compounds with metronidazole structures of all different categories were selected, of which 19 may not belong to any published patents or applications. Through prediction and personal experience, the difficulty of synthesizing these 20 new structures was analyzed, and compound 0001 was chosen as our synthetic target, and a series of structures (8a–l) similar to compound 0001 were synthesized. Finally, the inhibitory activities of these compounds against bacteria E. coli, P. aeruginosa, B. subtilis and S. aureus were determined. The results showed that compound 8a–l had obvious inhibitory activity against these four bacteria, which proved the accuracy of our compound generation model.
研究领域:
药理学
·
计算生物学
·
人工智能在药物研发中的应用
✓ 已验证
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2020
·
IF 5.88
Fan Zhang,Xiangsheng Han,Chuanlong Guo,Hui Yang,Jun Wang,Xiaochen Wu
Bacterial infections severely retard the wound healing process. Antibacterial drugs were loaded onto aramid nanofibers (ANFs) hydrogels through a convenient strategy to fabricate dressings for bacterial infected wound healing. ANFs hydrogels owned good mechanical properties, high water content (>98%), high water adsorption property (>10,000%) and good water retention ability (a water retention of >5000% after incubating at RH 30% for 8 h), capable to absorb and retain the wound exudate to form a moist environment. The incorporation of antibacterial drugs promised the ANFs hydrogels with durable antibacterial properties. Furthermore, the drug loaded ANFs hydrogels had no cytotoxicity nor hemolytic potential. In vivo skin wound healing results confirmed that the composite hydrogels accelerated the healing rate of infection wounds on mice and were suitable as a potential anti-infective wound dressing.
研究领域:
生物材料学
·
医学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）