PLANT PHYSIOLOGY
·
2024
·
IF 6.5
Chen Kai,Guo Dongdong,Yan Jiji,Zhang Huijuan,He Zhang,Wang Chunxiao,Tang Wensi,Chen Jun,Xu Zhaoshi,Ma Youzhi,Chen Ming
Soybean (Glycine max [L.] Merr.) is a valuable oil crop but is also highly susceptible to environmental stress. Thus, developing approaches to enhance soybean stress resistance is vital to soybean yield improvement. In previous studies, transcription factor Alfin has been shown to serve as an epigenetic regulator of plant growth and development. However, no studies on Alfin have yet been reported in soybean. In this study, the endoplasmic reticulum (ER) stress- and reactive oxygen species (ROS)-related GmAlfin09 was identified. Screening of genes co-expressed with GmAlfin09 unexpectedly led to the identification of soybean peroxidase 6 (GmPRDX6). Further analyses revealed that both GmAlfin09 and GmPRDX6 were responsive to ER stress, with GmPRDX6 localizing to the ER under stress. Promoter binding experiments confirmed the ability of GmAlfin09 to bind to the GmPRDX6 promoter directly. When GmAlfin09 and GmPRDX6 were overexpressed in soybean, enhanced ER stress resistance and decreased ROS levels were observed. Together, these findings suggest that GmAlfin09 promotes the upregulation of GmPRDX6, and GmPRDX6 subsequently localizes to the ER, reduces ROS levels, promotes ER homeostasis, and ensures the normal growth of soybean even under ER stress. This study highlights a vital target gene for future molecular breeding of stress-resistant soybean lines.
研究领域:
植物生物学
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分子遗传学
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农业科学
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产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2020
·
IF 4.303
Mingyi Yang,Zisheng Luo,Sunan Gao,Tarun Belwal,Lei Wang,Ming Qi,Zhaojun Ban,Bin Wu,Fengzhong Wang,Li Li
Plant cuticular wax is the first barrier to resist biotic and abiotic stress. However, little is known about the compositional differences between the epicuticular and intracuticular wax in grape berry. The compositional, morphological and functional features of cuticular wax in grape berries of Vitis vinifera cv. ‘Kyoho’, ‘Muscat Hamburg’, ‘Redglobe’, and ‘Zuijinxiang’ were investigated. The total and epicuticular wax of four berries were mainly composed of terpenoids, hydrocarbons, alcohols, fatty acids and esters. Oleanolic acid was the most abundant terpenoid among the four cultivars. Scanning electron microscopy revealed the crystalline flakes structure of the cuticular wax. Additionally, the removal of epicuticular wax accelerated the weight loss, browning, and softening of grape berries, indicating the plastic-wrap-like effect of the cuticular wax on postharvest quality. This study provided the theoretical basis for further application of the fruit wax or waxy analogue.
研究领域:
植物学
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园艺学
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食品科学
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产品关联
DOI
高影响因子
iScience
·
2023
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IF 5.8
Yuanyuan Zhang,Jie Zheng,Shiraku Margaret Linyerera,Richard Odongo Magwanga,Yuqing Hou,Yuhong Wang,Yanchao Xu,Aziz Khan,Shuxun Yu,Zhongli Zhou,Fang Liu,Xiaoyan Cai
Summary The 5′-deoxyadenosine deaminase (DADD), a member of the amidohydrolase family regulates biological purine metabolism. In this study, bioinformatic analysis, overexpression and knockdown of GhdadD gene were detected to identify its potential role in drought and salt stress tolerance. The results revealed that GhdadD was induced by ABA, Auxin, MBS and light responsive elements. In transgenic Arabidopsis, seed germination rate and root length were increased under drought or salt stress. GhdadD overexpressed seedlings resulted in higher plant height, less leaf damage and lower ion permeability. The expression of osmotic stress and ABA-responsive genes were up regulated. While in GhdadD -silenced cotton seedlings, CAT, SOD activity and soluble sugar content were reduced, MDA content was increased, and the stoma opening was depressed under drought or salt stress. Some osmics stress marker genes were also up regulated. These data indicating that GhdadD enhanced plant resistance to drought and salt stress through ABA pathways.
研究领域:
植物科学
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分子生物学
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胁迫生理学
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产品关联
DOI
PubMed
高影响因子
Open Life Sciences
·
2024
·Vol. 19
·期 1
·
IF 1.7
Muqing Ma,Jinbao Gu,Zhen-Yu Wang
As a gateway for gas exchange, pores regulate the transport of air and water in carbon assimilation, respiration, and transpiration to quickly adapt to environmental changes. Therefore, the study of stomatal movement characteristics of plants is helpful to strengthen the understanding of the mechanism of plant response to multi-environmental stress, and can improve the function of plant resistance to stresses. The stomatal movement of Arabidopsis leaves was observed by staining the stomata with rhodamine 6G, but this method has not been reported in other plant leaf stomata studies. Taking cassava as an example, the correlation between cassava stomatal movement and cassava response to stress was observed by using and improving the staining method. Rhodamine 6G is a biological stain widely used in cell biology and molecular biology. It was found that 1 μM rhodamine 6G could stain the stomata of cassava without affecting stomatal movement ( n = 109, p < 0.05). In addition, we proposed that stomata fixed with 4% concentration of formaldehyde after staining were closest to the stomatal morphology of cassava epidermis, so as to observe stomatal movement under different environmental stresses more accurately. Previous methods of measuring stomatal pore size by autofluorescence of cell wall needs to fix the cells for 6 h, but Rhodamine staining can only be observed in 2 min, which greatly improves the experimental efficiency. Compared with the traditional exfoliation method (e.g., Arabidopsis ), this method can reduce the damage of the leaves and observe the stomata of the whole leaves more completely, so that the experimental results are more complete. In addition, the method enables continuous leaf processing and observation. Using this method, we further compared four different cassava varieties (i.e., KU50, SC16, SC8, and SC205) and found that there are differences in stomatal density (SD) among cassava varieties, and the difference in the SD directly affects the stress resistance of cassava ( n = 107, p < 0.001). This finding has important implications for studying the mechanism of plant response to environmental stress through stomata.
研究领域:
植物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Xuecheng Li,Changyi Li,Ziyu Chen,Jiahui Wang,Jie Sun,Jun Yao,Ke Chen,Zhenghui Li,Hengpeng Ye
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous at relatively high concentrations by atmospheric deposition, and they are threatening to the environment. In this study, the toxicity of naphthalene on tall fescue and its potential responding mechanism was first studied by integrating approaches. Tall fescue seedlings were exposed to 0, 20, and 100 mg L −1 naphthalene in a hydroponic environment for 9 days, and toxic effects were observed by the studies of general physiological studies, chlorophyll fluorescence, and root morphology. Additionally, Ultra Performance Liquid Chromatography - Electrospray Ionization - High-Resolution Mass Spectrometry (UPLC-ESI-HRMS) was used to depict metabolic profiles of tall fescue under different exposure durations of naphthalene, and the intrinsic molecular mechanism of tall fescue resistance to abiotic stresses. Tall fescue shoots were more sensitive to the toxicity of naphthalene than roots. Low-level exposure to naphthalene inhibited the electron transport from the oxygen-evolving complex (OEC) to D1 protein in tall fescue shoots but induced the growth of roots. Naphthalene induced metabolic change of tall fescue roots in 12 h, and tall fescue roots maintained the level of sphingolipids after long-term exposure to naphthalene, which may play important roles in plant resistance to abiotic stresses.
研究领域:
环境科学
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植物生理学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
Journal of Fungi
·
2025
·Vol. 11
·期 7
·542
·
IF 4
Linhua Cao,Xiaoteng Shi,Tuo Li,Yang Liu,Tuokai Wang,Bozheng Lin,Dongyang Liu,Qirong Shen
Fusaric acid (FSA) is a mycotoxin produced by pathogenicFusariumspecies that inhibits the growth of various beneficial microbes. In this study, we investigated the molecular mechanisms by whichTrichoderma harzianumNJAU4742 (Th), a beneficial fungus, responds to FSA-induced stress. Here, by combining a transcriptome analysis, a gene knockout, and physiological data measurements, our study investigated the molecular mechanisms underlying the response ofTrichoderma harzianumNJAU4742 (Th) to FSA stress. The results showed that FSA can induce severe oxidative stress inTh, and an aldehyde dehydrogenase (Thaldh3) inThplays a critical role in alleviating FSA stress. DeletingThaldh3significantly decreased the γ-aminobutyrate (GABA) content, causing more severe oxidative damage inTh. Furthermore, we also provide evidence demonstrating thatThaldh3alleviates FSA stress by enhancing the activities of key enzymes involved in the tricarboxylic acid cycle and ATP content. A pot experiment showed that an enhanced tolerance to FSA increased theThbiomass, strengthening its antagonistic capacity against pathogens and reducing the disease index in tomatoes. In conclusion, these observations provide new insight into the role of beneficial microbes in promoting plant health.
研究领域:
微生物学
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植物病理学
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生物化学与分子生物学
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环境微生物学
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农业科学
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DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-13
·
IF 14.7
Tang Si,Liu Yaqing,Zhu Jianming,Cheng Xueyu,Liu Lu,Hammerschmidt Katrin,Zhou Jin,Cai Zhonghua
Understanding how organisms have adapted to persist in unpredictable environments is a fundamental goal in biology. Bet hedging, an evolutionary adaptation observed from microbes to humans, facilitates reproduction and population persistence in randomly fluctuating environments. Despite its prevalence, empirical evidence in microalgae, crucial primary producers and carbon sinks, is lacking. Here, we report a bet-hedging strategy in the unicellular microalga Haematococcus pluvialis . We show that isogenic populations reversibly diversify into heterophenotypic mobile and non-mobile cells independently of environmental conditions, likely driven by stochastic gene expression. Mobile cells grow faster but are stress-sensitive, while non-mobile cells prioritise stress resistance over growth. This is due to shifts from growth-promoting activities (cell division, photosynthesis) to resilience-promoting processes (thickened cell wall, cell enlargement, aggregation, accumulation of antioxidant and energy-storing compounds). Our results provide empirical evidence for bet hedging in a microalga, indicating the potential for adaptation to current and future environmental conditions and consequently conservation of ecosystem functions.
研究领域:
生物学
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生态学
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微生物学
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进化生物学
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产品关联
DOI
PubMed
高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
·
2024
·
IF 7.1
Yiming Zhou,Mengting Zhang,Siyu Lu,Li Liu,Zhigui Duan,Fang Wei,Guolin Li
Purpose While reactive oxygen species (ROS) have been identified as key redox signaling agents contributing to aging process, which and how specific oxidants trigger healthy longevity remain unclear. This paper aimed to explore the precise role and signaling mechanism of superoxide (O 2 •− ) in health and longevity. Methods A tool for precise regulation of O 2 •− levels in vivo was developed based on the inhibition of superoxide dismutase 1 (SOD1) by tetrathiomolybdate (TM) in Caenorhabditis elegans ( C. elegans ). Then, we examined the effects of TM on lifespan, reproduction, lipofuscin accumulation, mobility, and stress resistance. Finally, the signaling mechanism for longevity induced by TM-O 2 •− was screened by transcriptome analysis and tested in sod-1 and argk-1 RNAi strains, sod-2 , sod-3 , and daf-16 mutants. Results TM promoted longevity in C. elegans with a concomitant extension of healthy lifespan as indicated by increasing fertility and mobility and reducing lipofuscin accumulation, as well as enhanced resistance to different abiotic stresses. Mechanically, TM could precisely regulate O 2 •− levels in nematodes via modulating SOD1 activity. An O 2 •− scavenger Mn(III)TBAP abolished TM-induced lifespan extension, while an O 2 •− generator paraquat at low concentration mimicked the life prolongation effects. The longevity in TM-treated worms was abolished by sod-1 RNAi but was not affected in sod-2 or sod-3 mutants. Further transcriptome analysis revealed arginine kinase ARGK-1 and its downstream insulin/insulin-like growth factor 1 signaling (IIS) as potential effectors for TM-O 2 •‾ -induced longevity, and argk-1 RNAi or daf-16 mutant nullified the longevity. Conclusions These findings indicate that it is feasible to precisely control specific oxidant in vivo and O 2 •− orchestrates TM-induced health and longevity in C. elegans via ARGK-1-IIS axis.
研究领域:
衰老
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分子生物学
·
遗传学
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2023
·Vol. 15
·期 32
·13437-13449
·
IF 6.7
Hengjie Yu,Dan Luo,Sam Fong Yau Li,Maozhen Qu,Da Liu,Yingchao He,Fang Cheng
Crops are constantly challenged by different environmental conditions. Seed treatment using nanomaterials is a cost-effective and environmentally friendly solution for environmental stress mitigation in crop plants. Here, 56 seed nanopriming treatments are used to alleviate environmental stresses in maize. Seven selected nanopriming treatments significantly increase the stress resistance index (SRI) by 13.9% and 12.6% under salinity stress and combined heat–drought stress, respectively. Metabolomics data reveal that ZnO nanopriming treatment, with the highest SRI value, mainly regulates the pathways of amino acid metabolism, secondary metabolite synthesis, carbohydrate metabolism, and translation. Understanding the mechanism of seed nanopriming is still difficult due to the variety of nanomaterials and the complexity of interactions between nanomaterials and plants. Using the nanopriming data, we present an interpretable structure–activity relationship (ISAR) approach based on interpretable machine learning for predicting and understanding its stress mitigation effects. The post hoc and model-based interpretation approaches of machine learning are integrated to provide complementary advantages and may yield more illuminating or trustworthy results for researchers or policymakers. The concentration, size, and zeta potential of nanoparticles are identified as dominant factors for correlating root dry weight under salinity stress, and their effects and interactions are explained. Additionally, a web-based interactive tool is developed for offering prediction-level interpretation and gathering more details about a specific nanopriming treatment. This work offers a promising framework for accelerating the agricultural applications of nanomaterials and may contribute to nanosafety assessment.
研究领域:
农业
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环境科学
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机器学习
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植物科学
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产品关联
DOI
PubMed
高影响因子
BIOTECHNOLOGY LETTERS
·
2021
·Vol. 43
·期 3
·677-690
·
IF 2.461
Zhang Chaolei,Wang Chao,Zhao Shan,Xiu Zhilong
Objectives Probiotics ( Bacillus subtilis 04178) were entrapped in alginate-chitosan microcapsules by high-voltage electrostatic process. The encapsulation pattern was established as entrapped low density cells with culture (ELDC wc ). The performance of ELDC wc cells was investigated against stress environments of simulated digestive fluids. Results After incubation in simulated gastric (pH 2.5) and intestinal fluids (4% bile salt) for 2 h, the survival rate of ELDC wc cells (18.19% and 27.54%) was significantly higher than that of the free cells (0.0000009% and 0.0005%). The reason why B. subtilis embedded in microcapsules can resist the stress environments was that the mass production of extracellular proteins and polysaccharides prompted B. subtilis to form cell aggregates. The production of extracellular proteins and polysaccharides were regulated by the concentration of c-di-GMP and the expression of ydaJKLMN operon, abbA , sinI , slrA , slrB , abrR and sinR . Conclusions c-di-GMP is important for the production of extracellular polymer substance to enhance probiotic viability in stress environments.
研究领域:
微生物学
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生物技术
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Tiange Ma,Xingjiang Li,Manuel Montalbán-López,Xuefeng Wu,Zhi Zheng,Dongdong Mu
Lactococcus lactis is a crucial food-grade cell factory for secreting valuable peptides and proteins primarily via the Sec-dependent pathway. YidC, a membrane insertase, facilitates protein insertion into the lipid membrane for the translocation. However, the mechanistic details of how YidC affects protein secretion in L. lactis remain elusive. This study investigates the effects of deleting yidC1/yidC2 on L. lactis phenotypes and protein secretion. Compared to the original strain, deleting yidC2 significantly decreased the relative biomass, electroporation efficiency, and F-ATP activity by 25%, 47%, and 33%, respectively, and weakened growth and stress resistance, whereas deleting yidC1 had a minimal impact. The absence of either yidC1 or yidC2 reduced target proteins secretion. Meanwhile, there is a considerable alteration in the transcription levels of genes involved in the secretion pathway, with secY transcription increasing over 135-fold. Our results provide a theoretical foundation for further improving target protein secretion and investigating the YidC function.
研究领域:
微生物学
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生物技术
·
蛋白质工程
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产品关联
DOI
PubMed
高影响因子
Journal of Fungi
·
2024
·Vol. 10
·期 8
·521
·
IF 4.2
Guang Wang,Xu Zhang,Guangzu Du,Wenqian Wang,Yunhao Yao,Sitong Jin,Haosheng Cai,Yuejin Peng,Bin Chen
Metarhizium rileyiis a wide spread insect fungi with a good biocontrol potentiality to various pests, particularly noctuid insects. However, it is characterized by its slow growth, its sensitivity to abiotic stress, and the slow speed of kill to pests, which hinder its use compared with other entomopathogenic fungi. In this study, the responses ofM. rileyito eight types of lipids were observed; among the lipids, oleic acid and linoleic acid significantly promoted the growth and development ofM. rileyiand enhanced its stress tolerances and virulence. An additional mechanistic study demonstrated that exogenous oleic acid and linoleic acid significantly improved the conidial germination, appressorium formation, cuticle degradation, and cuticle infection, which appear to be largely dependent on the up-regulation of gene expression in growth, development, protective, and cuticle-degrading enzymes. In conclusion, exogenous oleic acid and linoleic acid enhanced the stress tolerances and virulence ofM. rileyivia protecting conidial germination and promoting cuticle infection. These results provide new insights for the biopesticide development ofM. rileyi.
研究领域:
昆虫学
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微生物学
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生物技术
·
害虫管理
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）