Asian Journal of Pharmaceutical Sciences
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2013
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IF 0
Xiaofan Wang,Xu Zhao,Liqiang Gu,Yuanyuan Zhang,Kaishun Bi,Xiaohui Chen
A novel study using LC–MS (Liquid chromatography tandem mass spectrometry) coupled with multivariate data analysis and bioactivity evaluation was established for discrimination of aqueous extract and vinegar extract of Shixiao San. Batches of these two kinds of samples were subjected to analysis, and the datasets of sample codes, t R - m / z pairs and ion intensities were processed with principal component analysis (PCA). The result of score plot showed a clear classification of the aqueous and vinegar groups. And the chemical markers having great contributions to the differentiation were screened out on the loading plot. The identities of the chemical markers were performed by comparing the mass fragments and retention times with those of reference compounds and/or the known compounds published in the literatures. Based on the proposed strategy, quercetin-3-O-neohesperidoside, isorhamnetin-3-O-neohespeeridoside, kaempferol-3-O-neohesperidoside, isorhamnetin-3-O-rutinoside and isorhamnetin-3-O-(2G-α-l-rhamnosyl)-rutinoside were explored as representative markers in distinguishing the vinegar extract from the aqueous extract. The anti-hyperlipidemic activities of two processed extracts of Shixiao San were examined on serum levels of lipids, lipoprotein and blood antioxidant enzymes in a rat hyperlipidemia model, and the vinegary extract, exerting strong lipid-lowering and antioxidative effects, was superior to the aqueous extract. Therefore, boiling with vinegary was predicted as the greatest processing procedure for anti-hyperlipidemic effect of Shixiao San. Furthermore, combining the changes in the metabolic profiling and bioactivity evaluation, the five representative markers may be related to the observed anti-hyperlipidemic effect.
研究领域:
中药学
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代谢组学
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生物信息学
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产品关联
DOI
ULTRASONICS SONOCHEMISTRY
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2024
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IF 8.7
Zihao Chen,Chuanju Wang,Jiarong Su,Guixin Liang,Shaofan Tan,Yongguang Bi,Fansheng Kong,Zhong Wang
In this research, the extraction process of polysaccharides from Pithecellobium clypearia Benth (PCBPs) was optimized using dual-frequency ultrasound-assisted extraction (DUAE). The biological activities of PCBPs were investigated by in vitro antioxidant, hypoglycemic, and anti-hyperlipidemic assay. High-performance anion-exchange chromatography, high-performance gel permeation chromatography, SEM, UV–Vis spectroscopy, and FT-IR spectra were used to analyze the monosaccharide composition, molecular weight, microscopic morphology, and characteristic structure of PCBPs. The results showed that the maximum extraction rate of PCBPs was 9.90 ± 0.16% when the ultrasonic time was 8 min, the liquid-to-material ratio was 32 mL/g, and the ultrasonic power was 510 W. The PCBPs also possessed excellent in vitro antioxidant, hypoglycemic, and anti-hyperlipidemic activities. In addition, the average molecular weight of PCBPs was 15.07 kDa. PCBPs consisted of rhamnose, arabinose, galactose, glucose, xylose, mannose, and glucuronic acid, with the molar ratios of 11.07%, 18.54%, 48.17%, 10.44%, 4.62%, 4.96%, and 2.20%, respectively. Moreover, the results of SEM showed that PCBPs mainly showed a fine spherical mesh structure. The above studies provided a valuable theoretical basis for the subsequent in-depth study of PCBPs.
研究领域:
植物化学
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药理学
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食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2024
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IF 4.5
Naiyer Shahzad,Ibrahim Abdel Aziz Ibrahim,Abdullah R.Alzahrani,Imran Shahid,Alaa Hisham Falemban,Ibrahim M. Alanazi,Ghazi A.Bamagous,Saeed S. Al-Ghamdi,Mohammad Tarique Imam,Nehal Mohsin,T. Indumathi,Palanisamy Arulselvan
ABSTRACT Diabetes mellitus (DM), a multifactorial chronic health condition, affects a sizable portion of the global population, and more people are expected to contract it in the future, according to the World Health Organisation (WHO). Diabetes mellitus can be treated with conventional drugs, but most of the medications have a variety of side effects. The use of nanocomposites (NCs) to treat diabetes has been prioritized in this scenario. In this study, Ag-CuO NCs were synthesized using a green method using Mentha spicata leaf extract and their physicochemical properties were investigated with a variety of analytical techniques. According to an extensive in vivo and in vitro analysis of the biological activities of as-synthesized Ag-CuO NCs, Ag-CuO NCs possess effective antibacterial, anti-diabetic, and anti-hyperlipidemic characteristics. When Ag-CuO NCs are administered to STZ-induced animals in a concentration-based manner, the blood levels of inflammatory and liver marker enzymes are reduced and antioxidant enzyme levels are increased. Besides, the carbon-coated Ag-CuO NCs exhibit excellent sensing activity with a limit of detection of 86 nM against Vitamin-C. This study reveals that Ag-CuO NCs derived from Mentha spicata may, therefore, prove to be a very successful anti-diabetic and biosensor candidate in the future.
研究领域:
纳米技术
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药理学
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生物传感
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药物化学
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生物医学工程
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2024
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IF 5.6
Chen Yang,Jingjing Li,Tingting Luo,Juan Tu,Tianyu Zhong,Yiqi Zhang,Xixi Liang,Liping Zhang,Zhiqiang Zhang,Jianming Wang
Oat bran polysaccharide (OBP), which can lower serum lipid levels and was developed as a safe and effective functional diet to treat cardiovascular diseases, was obtained using ultrasonic-microwave assisted extraction (UME). A maximum OBP extraction efficiency could be achieved when oat bran was processed with 639 W of microwave power, 36:1 of liquid-solid ratio, pH value of 10, and 18 min of UME processing time, according to statistical screening and optimization experiments. When UME was used instead of the standard hot water extraction method, the extraction time was slashed by 6.67 times, the yield of OBP nearly doubled, and OBP activity was unaffected. In vivo rat results showed that the significant anti-hyperlipidaemia activity of OBP was attributed to a decline in triglycerides , total cholesterol , and LDL-C as well as a rise in HDL-C. This is comparable to the positive group outcomes from the use of commercial β-glucan and simvastatin treated group, which may lessen liver fatty lesions and prevent the storage of fat. In conclusion, it is proposed that OBP extraction utilizing UME is a feasible method for anti-hyperlipidemic foods.
研究领域:
食品科学
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药理学
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营养学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）