Xiaoting Li,Beibei Chen,Man He,Han Wang,Guangyang Xiao,Bin Yang,Bin Hu
In this work, we demonstrate a novel method based on inductively coupled plasma mass spectrometry (ICP-MS) detection with gold nanoparticles (Au NPs) and quantum dots (QDs) labeling for the simultaneous counting of two circulating tumor cell lines (MCF-7 and HepG2 cells) in human blood . MCF-7 and HepG2 cells were captured by magnetic beads coupled with anti-EpCAM and then specifically labeled by CdSe QDs-anti-ASGPR and Au NPs-anti-MUC1, respectively, which were used as signal probes for ICP-MS measurement. Under the optimal experimental conditions, the limits of detection of 50 MCF-7, 89 HepG2 cells and the linear ranges of 200-40000 MCF-7, 300-30000 HepG2 cells were obtained, and the relative standard deviations for seven replicate detections of 800 MCF-7 and HepG2 cells were 4.6% and 5.7%, respectively. This method has the advantages of high sensitivity, low sample consumption, wide linear range and can be extended to the simultaneous detection of multiple CTC lines in human peripheral blood.
Biomarkers detection is of great significance in disease screening, early diagnosis and treatment responses. Herein, a plasmonic MXene induced multi-signal readout sensing platform was proposed for portable and ultrasensitive human anti-asialoglycoprotein receptor (human anti-ASGPR) detection, a biomarker for hepatitis. The plasmonic nanohybrid Ti 3 C 2 [email protected] prepared by decorating Ti 3 C 2 MXene with copper nano-clusters (Cu NCs) presented outstanding photothermal property and catalyst activity, which was developed as a signal reporter and participate in immune recognition event. The Ti 3 C 2 [email protected] induced visual color change from moderate blue to deep blue in the oxide-reduce methylene blue (MB-MBH 2 ) colorimetric system due to Cu NCs catalyzed more methylene blue (MB) produce. And the formation of Ti 3 C 2 [email protected] /MB complex presented brilliant photo-to-thermal conversion ability, under near infrared (NIR) laser radiation, enhanced photothermal effect from Ti 3 C 2 [email protected] /MB complex triggered significantly amplified temperature elevation in a temperature-responsive soft electronic devices (SED). Simultaneously, increased temperature greatly affected the conductivity variation in SED, this can be monitored by a commercially available digital multimeter (DMM) with resistance readout and transferred the laser stimulus into measurable electrical signal. Overall, such a new multi-signal readout biosensor realized sensitive target detection with high precision, low cost and simple operation.
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