PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2025
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IF 4
Yan Wang,Zhengxuan Wang,Yaping Liu,Charles Brennan,Jueting Liu,Jinhong Zhou,Wenbing Zhou,Guiguang Cheng
Natural polysaccharides have been attracted increasing attention in biopesticides for the antiviral effects. Tobacco leaves enriched the polysaccharides and had the potential against tobacco mosaic virus (TMV), however, the chemical structure and anti-TMV effect of polysaccharides from discarded tobacco leaves (DTCP) are still unclear. In this study, an optimized condition for extraction time of 3.5 h, temperature of 90 °C, and solid-liquid ratio of 1:45 led to the maximum extraction value of 21.11 % for DTCP. Subsequently, a homogeneous polysaccharide (DTP) of 3061 Da was purified from DTCP by DEAE and Sephadex G-100 column. The major monosaccharide composition of DTP was glucose (Glc), glucuronic acid (GlcA), galactose (Gal), and galacturonic acid (GalA) in a molar ratio of 3.9:2.6:1.06:0.84. NMR, methylation and IR analysis showed that DTP was a polysaccharide with →4)- β -D-Galp-(1 → 4)- β -D-Galp-(1 → 4)- β -D-Glcp as the main chain, and its branching points were composed of α -D-Glcp-(1→, α -D-GlcpA-(1→ and α -D-GalpA-(1→. Furthermore, DTP exhibited significant inactive and protective effects against TMV with the inhibitory rates of 80.40 % and 76.18 % at 500 μg/mL, respectively. DTP also interfered with the polymerization of TMV-CP to limit its self-assembly. Additionally, DTP could increase the superoxide dismutase, catalase, peroxidase and phenylalanine ammonia lyase activities of tobacco plants by 1.83, 2.73, 3.69 and 4.84 times, respectively, and could alleviate the hypersensitive response and systemic acquired resistance. Hence, DTP could serve as a new plant-derived pesticide for controlling TMV in agriculture.
研究领域:
植物学
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病毒学
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农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
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IF 6.1
Shunyu Xiang,Xiaoyan Wang,Shiqi Peng,Xinke Kang,Jing Wang,Liyuan Peng,Xiaozhou Ma,Jin Huang,Xianchao Sun
The application of antiplant virus agents on leaf surfaces faces challenges due to their vulnerability to wear, instability, and limited duration, which in turn jeopardizes plant health and yield. In recent years, high-aspect-ratio nanomaterials have gained prominence as powerful carriers for disease treatment, thanks to their exceptional penetrability and precise drug delivery capabilities. Here, we synthesized a pH-responsive nanoimmune inducer (CNC-AMO) with strong leaf adhesion through a Schiff base reaction, achieved by grafting amino-oligosaccharides (AMOs) on the surface of aldehyde-based CNC (CNC–CHO). Fourier transform infrared spectrometry, zeta potential, X-ray photoelectron spectroscopy, X-ray diffraction, transmission electron microscopy, atomic force microscopy, scanning electron microscopy, thermogravimetric analysis, and elemental analysis were used to characterize the CNC-AMO. The CNC-AMO displayed the capability for pH-responsive AMO release, showcasing its potential for targeted and controlled delivery. When applied to plants, the CNC-AMO exhibited impressive anti-TMV efficacy during a weeklong observation period. Meanwhile, the CNC-AMO exhibited remarkable adhesion and scouring resistance on the surfaces of the plant leaves. We strongly believe that the synergy of environmentally friendly synthetic materials, efficient plant virus control, and streamlined scalability positions CNC-AMOs as a promising pesticide for plant virus therapy.
研究领域:
植物学
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纳米技术
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植物病理学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2020
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IF 2.751
Xiang Zhou,Yiqiang Ye,Shasha Liu,Wubin Shao,Liwei Liu,Song Yang,Zhibing Wu
Plant viral diseases, known as “plant cancer”, with high contagiosity can substantially reduce crop quality and yield. To identify potential anti- tobacco mosaic virus (TMV) agents with different mechanisms, a series of novel α-aminophosphonate derivatives containing a chalcone moiety were designed and synthesized. Bioassay results revealed that some target compounds exhibited improved curative activity against TMV in vivo, and the EC 50 value of compound B 3 was 356.7 mg L −1 . The activities of the defensive enzymes POD and CAT from tobacco leaves treated with B 3 and B 17 showed that these target compounds could improve the photosynthetic ability of the leaves and activate plant host resistance against TMV infection. The binding constant between B 3 and TMV Coat Protein (CP) (2.51 × 10 8 M −1 ), calculated by the fluorescence titration experiment and docking results, revealed that B 3 has a strong interaction with TMV CP. Further docking analysis revealed that B 3 was embedded between two layers of the TMV CP, which was consistent with the 2:1 binding mode of TMV CP and B 3 determined by the binding affinity experiment. The TEM morphological study of TMV treated with B 3 and B 17 indicated that this series of target compounds may trigger the disassembly of TMV by interacting directly with TMV CP. This study provides new insight for the discovery of antiviral compounds with two different mechanisms of action.
研究领域:
植物病毒学
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有机化学
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植物病理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Yueyang Zhang,Yue Jiang,Yina Jia,Xiaoyu Pan,Tianrun Zhao,Kaiyue Wang,He Yan,Zhiqing Ma
Background Omphalia lapidescens is a saprophytic and parasitic fungus belonging to the Polypora genus of Tricholomataceae . It has repellent, insecticidal, anti-inflammatory and immunomodulatory effects. Result This study found that the extract of O. lapidescens had significant anti-TMV activity, and the main active component was homopolysaccharide LW-1 by Bioassay-guided fractionation. LW-1 is a glucan with β-(1,3) glucoside bond as the main chain and β-(1,6) glucoside bond as the branch chain, with molecular weight in the range of 172,916–338,827 Da. The protective and inactive efficacies of LW-1(100 mg/L) against TMV were 78.10% and 48.20%, but had no direct effect on the morphology of TMV particles. The results of mechanism of action showed that LW-1 induced the increase of the activity of defense enzymes such as POD, SOD and PAL in Nicotiana glutinosa . The overexpression of resistance genes such as NPR1, PR1 and PR5 , and the increase of SA content. Further transcriptome sequencing showed that LW-1 activated MAPK signaling pathway, plant-pathogen interaction pathway and glucosinolide metabolic pathway in Arabidopsis thaliana . Besides, LW-1 induced crops resistance against plant pathogenic fungi. Conclusion Taken together, the anti-TMV mechanism of LW-1 was to activate MAPK signaling pathway, inducing overexpression of resistance genes, activating plant immune system, and improving the synthesis and accumulation of plant defencins such as glucosinolide. LW-1-induced plant disease resistance has the advantages of broad spectrum and long duration, which has the potential to be developed as a new antiviral agent or plant immune resistance inducer.
研究领域:
植物病理学
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分子生物学
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植物化学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）