Journal of Nanoelectronics and Optoelectronics
·
2017
·Vol. 12
·期 11
·1251-1257
·
IF 0.497
Zeng Lu,He Mou,Yang Ligang,Wang Shuping
In this paper, the fluorinated modification of calcium silicate hydrate (F-CSH), prepared by hydrothermal process, is reported. The prepared F-CSH was well characterized by nuclear magnetic resonance (NMR) and nitrogen (N2) adsorption studies. The detailed analysis revealed
that the fluorine (F) exists in the prepared material in two forms, i.e., attached to the surface of F-CSH as CaF2, and/or connected to Ca2+ in the interlayers of C–S–H. Moreover, the properties of moisture resistance and carbonation resistance are improved
in F-CSH compared to pure CSH. This research is of great significance on the application of various fluorine-doped inorganic compounds.
研究领域:
材料科学
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化学工程
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DOI
高影响因子
COMPOSITES PART B-ENGINEERING
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2025
·
IF 14.2
Qian Su,Kangli Yang,Zhiqing Yuan,Cancheng Li,Shoutong Meng,Xuyu Long,Rui He
Aiming at the problems of carbon fiber board (CFB), which is not waterproof and has poor UV resistance, a superhydrophobic coating with UV resistance and high-temperature resistance was innovatively prepared on CFB using an aqueous-phase polymer dispersion system by a highly efficient spraying method. The whole preparation system is highly environmentally friendly and safe. The results showed that the superhydrophobic carbon fiber board (SKCFB) could resist UV up to 85 h, could withstand high temperatures up to 300 °C, and had a water contact angle (WCA) of 159°. In addition, the abrasion resistance of SKCFB is 140 and 180 cycles under the action of 800 grit and 1200 grit sandpaper, respectively, for a load of 100 g, and 110 cycles under the action of 600 grit sandpaper. When carrying a load of 500 g, the 600 mesh, 800 mesh, and 1200 mesh sandpaper can be used for 40, 60, and 80 cycles of abrasion resistance, respectively. Compared with other samples of the same type, this SKCFB is simpler to prepare, has a greener and safer preparation route, and has better performance, plus the ability to control the appearance color as desired. This study provides a novel green, simple, and fast strategy for preparing superhydrophobic coatings on CFBs. At the same time, it opens a new path for preparing superhydrophobic coatings and enhances their application in industry.
研究领域:
材料科学
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环境科学
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产品关联
DOI
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2024
·
IF 3.2
Yao Xiao,Yukun Zhou,Yinggang Huang,Biao Li,Yaqi Ge,Zhenchun Xu,Chuansheng Wang,Huiguang Bian
In this paper, chitosan (CS), which is naturally degradable and low-cost, was incorporated into nitrile butadiene rubber (NBR), the effects of chitosan amount on the properties of the NBR composites were investigated, and the optimal amount of chitosan was determined. On this basis, CS, silica and Si69 were modified by ball milling and plasma to prepare NBR composites with higher oil resistance and high temperature resistance. The results showed that the overall properties of NBR composites filled with 2phr CS is better. FTIR and SEM demonstrated the surface polarity of ball milling combined with plasma co-modified CS/silica was weakened, the dispersion of fillers in the NBR matrix was promoted, the oil resistance and high-temperature resistance of the composites were enhanced, the cross-linking density and tear strength were improved by 28.12%, and 51.76% compared with that of the NBR composites without CS. The investigation on the oil and high-temperature resistance of NBR composites for blowout preventer cores has been carried out, which promotes the development of blowout preventer cores toward high safety, low cost, and sustainability. Highlights The effect of chitosan amount on nitrile butadiene rubber (NBR) composites was explored. Chitosan, silica, and coupling agent were modified by ball milling and plasma. The oil and high-temperature resistance of NBR composites were improved. The tear strength was increased by 28% under double destructive environment.
研究领域:
材料科学
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化学工程
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产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 138
·期 47
·51410
·
IF 3.125
Yanying Hao,Guyue Wang,Xiaoxue Shui,Anping Tian,Yong Yang,Jin Zhu
A series of bio-based diester plasticizers with various alkyl chain asymmetry and the same molecular weight were designed and synthesized, using renewable 5-hydroxymethyl-2-furancarboxylic acid (HFCA) as the raw material. The chemical structures of the HFCA-based plasticizers were characterized by Fourier transform infrared and nuclear magnetic resonance ( 1 H NMR and 13 C NMR). Besides, the influence of alkyl chain asymmetry on plasticization properties of the HFCA-based plasticizers in poly(vinyl chloride) (PVC) blends was also investigated. It was found that increasing the alkyl chain asymmetry of the diester plasticizer and keeping the molecular weight unchanged simultaneously could further improve its plasticizing efficiency, without sacrificing its volatility resistance and exudation resistance. The results showed that this study provided a new approach for further optimizing the overall properties of the asymmetric diester plasticizers.
研究领域:
材料科学
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化学工程
·
高分子化学
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产品关联
DOI
高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
·
2023
·
IF 7.4
Caihua Wang,Xiaofang Li,Wei Zhang,Wenxuan Liu,Ziwei Lv,Runlin Gui,Man Li,Yujia Li,Xiaomin Sun,Ping Liu,Xiaobin Fan,Shiyao Yang,Yuyan Xiong,Lu Qian
Excessive free fatty acids (FFAs) accumulation is a leading risk factor for the pathogenesis of insulin resistance (IR) in metabolic tissues, including the liver. Ethanolamine-phosphate phospho-lyase (ETNPPL), a newly identified metabolic enzyme , catalyzes phosphoethanolamine (PEA) to ammonia, inorganic phosphate, and acetaldehyde and is highly expressed in hepatic tissue. Whether it plays a role in regulating FFA-induced IR in hepatocytes has yet to be understood. In this study, we established an in vitro palmitic acid (PA)-induced IR model in human HepG2 cells and mouse AML12 cells with chronic treatment of PA. Next, we overexpressed ETNPPL by using lentivirus-mediated ectopic to investigate the effects of ETNPPL per se on IR without PA stimulation. We show that ETNPPL expression is significantly elevated in PA-induced IR and that silencing ETNPPL ameliorates this IR in hepatocytes. Inversely, overexpressing ETNPPL under normal conditions without PA promotes IR, reactive oxygen species generation, and ARG2 activation in both HepG2 and AML12 cells. Moreover, ETNPPL depletion markedly down-regulates ARG2 expression in hepatocytes. Besides, silencing ARG2 prevents ETNPPL-induced ROS accumulation and inhibition of autophagic flux and IR in hepatocytes. Finally, we found that phytopharmaceutical disruption of ETNPPL by quercetin ameliorates PA-induced IR in hepatocytes. Our study discloses that ETNPPL inhibiting autophagic flux mediates insulin resistance triggered by PA in hepatocytes via ARG2/ROS signaling cascade. Our findings provide novel insights into elucidating the pathogenesis of obesity-associated hepatic IR, suggesting that targeting ETNPPL might represent a potential approach for T2DM therapy.
研究领域:
代谢
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分子生物学
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细胞生物学
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内分泌学
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药理学
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产品关联
DOI
PubMed
高影响因子
Human Cell
·
2025
·Vol. 38
·期 3
·1-14
·
IF 3.1
Zhu Xiao-Yu,Li Pi-Song,Qu Hui,Ai Xing,Zhao Zi-Ting,He Jia-Bei
Radiotherapy resistance, is usually caused by enhanced tumor stemness and poses a significant challenge in treating breast cancer (BRCA). In this study, we investigated the molecular regulatory mechanism of radiotherapy sensitivity in BRCA associated with replication factor C4 (RFC4) and insulin-like growth factor 2 mRNA binding protein 2 mRNA Binding Protein 2 (IGF2BP2). RFC4 expression was increased in BRCA cell lines and tissues, and high RFC4 expression in BRCA patients predicted the occurrence of lymphatic metastasis. RFC4-specific short hairpin RNA sequences or RFC4 coding sequences were subsequently cloned and inserted into plasmid vectors to downregulate or upregulate RFC4 expression. Knockdown of RFC4 attenuated stemness, as evidenced by a reduction in sphere formation and the downregulation of CD44 and SOX2. RFC4 silencing inhibited migration and invasion, promoted apoptosis, and improved sensitivity to radiotherapy (4-Gy X-ray). The results were detected by a wound healing assay, a transwell assay, and flow cytometry. The overexpression of RFC4 had the opposite effect on BRCA cells. Like RFC4 expression, IGF2BP2 expression was also increased in the cancer tissues of breast cancer patients. The results of the dual luciferase assay and RIP assay confirmed the binding of IGF2BP2 to the RFC4 mRNA coding sequence. Knockdown of RFC4 eliminated the effects of IGF2BP2 overexpression on increasing cell viability, invasion, the expression of stemness markers and radioresistance, suggesting that the effect of RFC4 on the stemness of BRCA cells was regulated by IGF2BP2. In conclusion, we reported that RFC4, a key regulator of BRCA progression, promoted radioresistance in BRCA and was positively regulated by IGF2BP2.
研究领域:
肿瘤学
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分子生物学
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医学
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产品关联
DOI
PubMed
高影响因子
IMMUNITY
·
2024
·
IF 25.5
Jiping Sun,Youqin Zhang,Qingbing Zhang,Lin Hu,Linfeng Zhao,Hongdong Wang,Yue Yuan,Hongshen Niu,Dongdi Wang,Huasheng Zhang,Jianyue Liu,Xujiao Feng,Xiaohui Su,Ju Qiu,Jing Sun,Heping Xu,Catherine Zhang,Kathleen Wang,Yan Bi,Edgar G. Engleman,Lei Shen
Adipose tissue group 2 innate lymphoid cells (ILC2s) help maintain metabolic homeostasis by sustaining type 2 immunity and promoting adipose beiging. Although impairment of the ILC2 compartment contributes to obesity-associated insulin resistance, the underlying mechanisms have not been elucidated. Here, we found that ILC2s in obese mice and humans exhibited impaired liver kinase B1 (LKB1) activation. Genetic ablation of LKB1 disrupted ILC2 mitochondrial metabolism and suppressed ILC2 responses, resulting in exacerbated insulin resistance. Mechanistically, LKB1 deficiency induced aberrant PD-1 expression through activation of NFAT, which in turn enhanced mitophagy by suppressing Bcl-xL expression. Blockade of PD-1 restored the normal functions of ILC2s and reversed obesity-induced insulin resistance in mice. Collectively, these data present the LKB1-PD-1 axis as a promising therapeutic target for the treatment of metabolic disease.
研究领域:
免疫学
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代谢生物学
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医学
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DOI
PubMed
高影响因子
Microorganisms
·
2022
·Vol. 11
·期 1
·67
·
IF 4.926
Kejian Tian,Yue Yu,Qing Qiu,Xuejian Sun,Fanxing Meng,Yuanping Bi,Jinming Gu,Yibing Wang,Fenglin Zhang,Hongliang Huo
Bisphenol A (BPA) pollution poses an increasingly serious problem. BPA has been detected in a variety of environmental media and human tissues. Microbial degradation is an effective method of environmental BPA remediation. However, BPA is also biotoxic to microorganisms. In this study,Rhodococcus equiDSSKP-R-001 (R-001) was used to degrade BPA, and the effects of BPA on the growth metabolism, gene expression patterns, and toxicity-resistance mechanisms ofRhodococcus equiwere analyzed. The results showed that R-001 degraded 51.2% of 5 mg/L BPA and that 40 mg/L BPA was the maximum BPA concentration tolerated by strain R-001. Cytochrome P450 monooxygenase and multicopper oxidases played key roles in BPA degradation. However, BPA was toxic to strain R-001, exhibiting nonlinear inhibitory effects on the growth and metabolism of this bacterium. R-001 bacterial biomass, total protein content, and ATP content exhibited V-shaped trends as BPA concentration increased. The toxic effects of BPA included the downregulation of R-001 genes related to glycolysis/gluconeogenesis, pentose phosphate metabolism, and glyoxylate and dicarboxylate metabolism. Genes involved in aspects of the BPA-resistance response, such as base excision repair, osmoprotectant transport, iron-complex transport, and some energy metabolisms, were upregulated to mitigate the loss of energy associated with BPA exposure. This study helped to clarify the bacterial mechanisms involved in BPA biodegradation and toxicity resistance, and our results provide a theoretical basis for the application of strain R-001 in BPA pollution treatments.Keywords:Bisphenol A;Rhodococcus;biodegradation;toxicity resistance
研究领域:
微生物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Virulence
·
2025
·
IF 5.4
Zijing Liang,Shuoyue Wang,Xinchi Zhu,Jiale Ma,Huochun Yao,Zongfu Wu
Streptococcus suis is a Gram-positive pathogen causing septicaemia and meningitis in pigs and humans. However, how S. suis maintains a high bacterial load in the blood and brain is poorly understood. In this study, we found that a small RNA rss03 is predominantly present in S. suis, Streptococcus parasuis, and Streptococcus ruminantium, implying a conserved biological function. rss03 with a size of 303 nt mainly exists in S. suis sequence type (ST) 1 and epidemic ST7 strains that are responsible for human infections in China. Using MS2-affinity purification coupled with RNA sequencing (MAPS), proteomics analysis, and CopraRNA prediction, 14 direct targets of rss03 from an ST7 strain were identified. These direct targets mainly involve substance transport, transcriptional regulation, rRNA modification, and stress response. A more detailed analysis reveals that rss03 interacts with the coding region of glpF mRNA, and unexpectedly rss03 protects glpF mRNA from degradation by RNase J1. The GlpF protein is an aquaporin, contributes to S. suis oxidative stress resistance by H2O2 efflux, and facilitates bacterial survival in murine macrophages RAW264.7. Finally, we showed that rss03 and GlpF are required to maintain a high bacterial load in mouse blood and brain. Our study presents the first sRNA targetome in streptococci, enriches the knowledge of sRNA regulation in streptococci, and identifies pathways contributing to S. suis pathogenesis.
研究领域:
微生物学
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分子生物学
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感染病学
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产品关联
DOI
PubMed
高影响因子
Cell Death Discovery
·
2025
·Vol. 11
·期 1
·1-17
·
IF 7
Wei Guojiang,Huang Ying,Li Wenya,Xie Yuxin,Zhang Deyi,Niu Yuanjie,Zhao Yang
Metabolic reprogramming in prostate cancer has been widely recognized as a promoter of tumor progression and treatment resistance. This study investigated its association with ferroptosis resistance in prostate cancer and explored its therapeutic potential. In this study, we identified differences in the epithelial characteristics between normal prostate tissue and tissues of various types of prostate cancer using single-cell sequencing. Through transcription factor regulatory network analysis, we focused on the candidate transcription factor, SREBF1. We identified the differences in SREBF1 transcriptional activity and its association with ferroptosis, and further verified this association using hdWGCNA. We constructed a risk score based on SREBF1 target genes associated with the biochemical recurrence of prostate cancer by combining bulk RNA analysis. Finally, we verified the effects of the SREBPs inhibitor Betulin on the treatment of prostate cancer and its chemosensitization effect. We observed characteristic differences in fatty acid and cholesterol metabolism between normal prostate tissue and prostate cancer tissue, identifying high transcriptional activity of SREBF1 in prostate cancer tissue. This indicates that SREBF1 is crucial for the metabolic reprogramming of prostate cancer, and that its mediated metabolic changes promoted ferroptosis resistance in prostate cancer in multiple ways. SREBF1 target genes are associated with biochemical recurrence of prostate cancer. Finally, our experiments verified that SREBF1 inhibitors can significantly promote an increase in ROS, the decrease in GSH, and the decrease in mitochondrial membrane potential in prostate cancer cells and confirmed their chemosensitization effect in vivo. Our findings highlighted a close association between SREBF1 and ferroptosis resistance in prostate cancer. SREBF1 significantly influences metabolic reprogramming in prostate cancer cells, leading to ferroptosis resistance. Importantly, our results demonstrated that SREBF1 inhibitors can significantly enhance the therapeutic effect and chemosensitization of prostate cancer, suggesting a promising therapeutic potential for the treatment of prostate cancer.
研究领域:
肿瘤学
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分子生物学
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生物信息学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2025
·
IF 11.5
Xuan Li,Hanchen Zhang,Zheng Cao,Haihua Xiao,Chao Weng,Qingfeng Zheng
The clinical efficacy of platinum-based chemotherapeutics, such as cisplatin, has been compromised by the prevalent emergence of drug resistance. This has propelled the search for alternative metal-based anti-tumor agents. Herein, we introduce PolyRu, a novel amphiphilic polymer containing a ruthenium complex and thioketal bonds in its main chain, for lung cancer treatment. PolyRu can self-assemble into nanoparticles (NP@PolyRu) in aqueous solutions and degrade within the reactive oxygen species-rich tumor microenvironment. The ruthenium complex in PolyRu specifically targets mitochondria and induces cancer cell apoptosis. The efficacy of NP@PolyRu was validated in a patient-derived xenograft model of human lung cancer, where NP@PolyRu significantly suppressed tumor progression with favorable biocompatibility，and showed excellent anti-tumor immune effect. NP@PolyRu emerges as a promising candidate for addressing cancer drug resistance, signifying a substantial leap forward in the realm of metal polymer-based cancer therapeutics.
研究领域:
肿瘤学
·
药理学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Huashan Gao,Linlin Ding,Rui Liu,Xinhua Zheng,Xichao Xia,Fuan Wang,Jinxu Qi,Weishuang Tong,Yuanhao Qiu
Anoectochilus roxburghii is a traditional herb in China that can be potentially used to treat diabetes. A novel polysaccharide ARLP-W was isolated from Anoectochilus roxburghii by chromatography on DEAE-52 cellulose. Chemical analysis indicated that ARLP-W (8.1 × 10 4 Da) was mainly composed of mannose and glucose. The main linkages of glycosidic bonds of ARLP-W were β-1, 4-Manp and α-1, 4-Glcp. The terminal Glcp was connected to Manp-via O-3. RT-qPCR and western blotting analysis showed that ARLP-W caused a significant reduction in the levels of the key gluconeogenesis enzymes phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase (G6Pase) in the liver. The results of the insulin resistance tests indicated that ARLP-W increased glucose absorption. These results indicate that ARLP-W has a good therapeutic effect on type 2 diabetes and can assist with further development and application treatment of diabetes.
研究领域:
药理学
·
生物化学
·
糖尿病研究
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）