Cell Reports
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2020
·
IF 8.109
Shanshan Qiao,Li Bao,Kai Wang,Shanshan Sun,Mingfang Liao,Chang Liu,Nan Zhou,Ke Ma,Yuwei Zhang,Yihua Chen,Shuang-Jiang Liu,Hongwei Liu
Summary A beneficial gut Bacteroides -folate-liver pathway regulating lipid metabolism is demonstrated. Oral administration of a Ganoderma meroterpene derivative (GMD) ameliorates nonalcoholic hepatic steatosis in the liver of fa/fa rats by reducing endotoxemia, enhancing lipid oxidation, decreasing de novo lipogenesis, and suppressing lipid export from the liver. An altered gut microbiota with an increase of butyrate and folate plays a causative role in the effects of GMD. The commensal bacteria Bacteroides xylanisolvens , Bacteroides thetaiotaomicron , Bacteroides dorei , and Bacteroides uniformis, which are enriched by GMD, are major contributors to the increased gut folate. Administration of live B. xylanisolvens reduces hepatic steatosis and enhances the folate-mediated signaling pathways in mice. Knockout of the folate biosynthetic folp gene in B. xylanisolvens blocks its folate production and beneficial effects. This work confirms the therapeutic potential of GMD and B. xylanisolvens in alleviating nonalcoholic hepatic steatosis and provides evidence for benefits of the gut Bacteroides -folate-liver pathway.
研究领域:
胃肠病学
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微生物学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
Food & Function
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2019
·Vol. 10
·期 7
·4304-4314
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IF 3.241
Chunqing Ai,Pingrui Jiang,Yili Liu,Mengmeng Duan,Xiaona Sun,Tengrui Luo,Guoping Jiang,Shuang Song
Accumulating studies showed that the beneficial actions of polysaccharides were closely associated with an improvement of the gut microbiota, but mechanisms that link polysaccharides and gut microbiota alterations are ill defined. Alginate from Laminaria japonica (LJP-A) can avoid being digested by the upper digestive tract and reach the colon where it can improve the gut microbiota community. LJP-A increased the relative abundance of some Bacteroidaceae members, e.g. Bacteroides species. The distribution of Bacteroides species in the gut is specific to the individual, and the specific use of LJP-A by Bacteroides species can determine LJP-A-induced alterations of the intestinal Bacteroides community, in which carbon source-based syntrophic interactions occur in Bacteroides species of the human gut microbial ecosystem. Compared to other species, Bacteroides finegoldii responded more positively to LJP-A intervention, and its properties may have a close connection with the bioactivities of LJP-A. This study provides a rationale for personalized treatment with dietary polysaccharides that were optimally fermented within a particular individual.
研究领域:
微生物学
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营养学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xujiao Li,Yinan Chen,Lihua Song,Jiangmei Wang,Zibo Song,Xiaoyan Zhao,Changyan Zhou,Yan Wu
Two hydrolyzed fractions of tamarind seed polysaccharide (TSP), denoted ETSP1 (176.68 kDa) and ETSP2 (34.34 kDa), were prepared by partial degradation via endo- xyloglucanase, and then characterized and evaluated by simulated gastrointestinal digestion in vitro . The results showed that the hydrolyzed TSPs remained indigestible in gastric and small intestinal media, and were fermented by gut microbiota, similar to the native TSP (M w = 481.52 kDa). Although the degradation of hydrolyzed TSPs was accelerated during fermentation with a decreasing degree of polymerization, the content of produced total short-chain fatty acids (SCFAs) decreased. After fermentation, the gut microbiota composition was modified, esp. the Firmicutes/Bacteroidetes ratio decreased (1.06 vs. 0.96 vs. 0.80) with a decreasing degree of polymerization, which implied that the potential anti-obesity prebiotic effect was enhanced. At the genus level, hydrolyzed TSPs maintained similar roles as native TSP, including promoting beneficial bacteria ( Bifidobacterium , Parabacteroides , and Faecalibacterium ) and inhibiting enteropathogenic bacteria ( Escherichia-Shigella and Dorea ). Moreover, ETSP1 had additional potential due to abundant Bacteroides vulgatus (LDA = 4.68), and ETSP2 might perform better as related to Bacteroides xylanisolvens (LDA = 4.40). All these results indicated the prebiotic potential of hydrolyzed TSP with detailed information about changes in degradation and gut microbiota based on enzyme-hydrolysis.
研究领域:
食品科学
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营养学
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微生物学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Ciliang Guo,Shihai Zhang,Yeqing Wang,Meixia Li,Kan Ding
A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography. HAW1-2 was proved as a homogenous polysaccharide with a molecular weight ( Mw ) of 8.94 kDa. Monosaccharide composition results indicated that HAW1-2 was composed of arabinose, galactose and glucose. Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β- d -Glc p -(1→, →4)-β- d -Gal p -(1→, α-L-Ara f -(1→, →5)-α-L-Ara f -(1→, β- d -Glc p -(1→ and →4,6)-β- d -Glc p -(1→. Bioactivity test showed that HAW1-2 could significantly promote the growth of Bacteroides thetaiotamicron ( BT ), Bacteroides ovatus ( BO ) and Bifidobacterium longum ( BL ), which showed competition with the Bacteroides spp. under co-culture. Interestingly, Bacteroides spp. generated more acetic acids and propionic acids while BL only generated acetic acids. These results imply that polysaccharide HAW1-2 may be useful for human by modulating intestinal bacteria and producing short chain fatty acids.
研究领域:
食品科学
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微生物学
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营养学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
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2024
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IF 3.2
Jinghan Wang,Yanming Ren,Xingqian Ye,Huiling Zhang,Jinhu Tian
Goji berry ( Lycium barbarum L.) is a nutrient-rich fruit and has received enormous interest for its health benefits. The beneficial effects of goji berry are linked to the absorption of bioactive compounds within the gastrointestinal digestion process and colon fermentation. Nonetheless, how certain bioactive compounds were released, and metabolism changed of the consumption of whole goji berries were still unclear. Therefore, the present study aimed to evaluate the digestion characteristics of key bioactive compounds in whole goji berries with an in vitro digestion model, and the effects of whole goji berries on the structure of gut microbiota were also investigated. Results showed that a significant release of carbohydrates during the digestion process, peaking within the first 15 min of the intestinal phase (421.4 ± 5.82 mg GE/g, dry weight, respectively), was observed, and the phenolic release reached the highest in the first 15 min of the gastric phase. Meanwhile, the bioaccessibilities of phenolic compounds and carbohydrates were determined to be 63.87% and 80.40%, respectively, after intestinal digestion. In addition, the undigested fractions of goji berries could be further fermented to produce short-chain fatty acids, which decreased the colon pH value (from 7.38 to 6.71) as well as the Firmicutes/Bacteroidetes ratio. Moreover, the goji berries regulated the composition of gut microbiota by promoting beneficial bacteria such as Bacteroides , Parabacteroides , and Paraclostridium , whereas inhibiting the proliferation of harmful bacteria (e.g., Fusobacterium ). Our results indicated that the goji berry exhibited significant bioactivity during the digestion and fermentation stage and might provide some new insights into the utilization of goji berries in healthy food processing.
研究领域:
营养学
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食品科学
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微生物学
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植物化学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2019
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IF 3.579
Ruiqiu Zhao,Ninghui Cheng,Paul A. Nakata,Liyan Zhao,Qiuhui Hu
The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice. AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period. AAP feeding changed the intestinal environment in the mice. The pH value of the intestinal compartments decreased while SCFAs concentrations increased in AAP-fed groups, in a dose dependent manner, compared to the controls ( P < .05). High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio ( P < .05). The relative abundances of Porphyromonadaceae and Bacteroidaceae also increased in the AAP fed mice which positively correlated with an increase in serum IgA and IgG concentrations ( P < .05). The findings from this study show that AAP modulates the mouse gut microbiota and may contribute, at least in part, to some of the reported beneficial effects from the consumption of the mushroom, A. auricula .
研究领域:
微生物学
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营养学
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免疫学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
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IF 5.162
Junjun Li,Bing Pang,Ximei Yan,Xiaoya Shang,Xinzhong Hu,Junling Shi
Artemisia sphaerocephala Krasch polysaccharide (ASKP) and its two fractions—60P (branched xylan) and 60S (branched glucomannan), were subjected to simulated gastrointestinal digestion and in vitro fermentation by human fecal microbiota. The results showed that all polysaccharide fractions could transit through gastrointestinal tract without dramatic degradation and be utilized by gut microbiota. ASKP exhibited the highest depletion rate and highest capability to decrease the pH than its fractions. Meanwhile, 60S showed the stronger capability to increase the production of propionic acid and reduce the ratio of acetic acid to propionic acid. At the phylum level, all polysaccharides efficiently reduced the Firmicutes/Bacteroidetes ratio and relative abundance of Proteobacteria, with ASKP being the most capable to suppress the proliferation of Proteobacteria. At the genus level, ASKP and 60P markedly promoted the growth of Bacteroidetes , and 60S promoted the growth of Parabacteroides and Collinsella . Prediction on metabolic function revealed that polysaccharide administration could dramatically change the metabolic profile of bacteria compared with fructooligosaccharides. Besides, all the polysaccharides dramatically promoted the bile acid metabolism. Compared with 60S, ASKP and 60P showed stronger ability to suppress the metabolisms on carbohydrate and amino acid. In summary, both ASKP and its two fractions showed the prebiotic potentials.
研究领域:
食品科学
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微生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2025
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IF 12.4
Yonggan Sun,Shanshan Zhang,Tianxiang Ouyang,Shaoping Nie
Konjac glucomannan (KGM), a common food hydrocolloid widely used in the food industries, has broad biological activities, but its targeted effects on gut microbiota remain unclear. In this study, we used feces microbiota from 30 healthy volunteers, conducted an in vitro fermentation, employed multi-omics analysis to characterize the effect of KGM on the gut microbiota, and performed a stepwise culture system to investigate its microbial interactions. KGM suppressed the opportunistic pathogen (unclassified Enterobacteriaceae) and the accumulation of branched-chain fatty acids, p-cresol, sulfides, and indole. In contrast, KGM enriched the proliferation of some potentially beneficial bacteria ( Faecalibacterium and Parabacteroides ) and the accumulation of short-chain fatty acids, as well as volatile organic metabolites, such as butanoic acid propyl ester. Notably, F. prausnitzii and P. distasonis were specifically enriched, but neither could utilize KGM directly. The primary degrader, such as Bacteroides ovatus , can degrade KGM and promote the growth of F. prausnitzii and P. distasonis . Our findings offer novel insights into KGM-microbiota interactions while establishing a scientific foundation for developing KGM-based functional foods targeting gut health enhancement.
研究领域:
食品科学
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微生物学
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营养学
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生物信息学
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功能食品
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代谢组学
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益生元
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DOI
高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Zi Ye,Leilei Yu,Chuan Zhang,Yuhang Gao,Jianxin Zhao,Arjan Narbad,Wei Chen,Qixiao Zhai,Fengwei Tian
Flammulina velutipes , a widely cultivated species of edible fungus , exhibits diverse functional activities attributed to its polysaccharides . In this study, we employed an in vitro model to investigate the impact of F. velutipes polysaccharides (FVP) fermentation on gut microbiota , with a particular focus on Bacteroides . FVP fermentation resulted in the proliferation of microbiota associated with short-chain fatty acid (SCFA) metabolism and suppression of Escherichia-Shigella . Bacteroides emerged as potential primary degraders of FVP, with species-level analysis identifying the preference of B. thetaiotaomicron and B. intestinalis in FVP degradation. Metabolomics analysis revealed significant increases in hypoxanthine and 7-methyladenine contents, with histidine metabolism emerging as the most enriched pathway. B. nordii and B. xylanisolvens exhibited the most influence on amino acid and SCFA metabolism. Understanding the mechanisms by which gut microbiota metabolize FVP can provide valuable insights into the potential of FVP to promote intestinal health and disease prevention.
研究领域:
微生物学
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代谢组学
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营养学
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DOI
PubMed
高影响因子
Food & Function
·
2023
·Vol. 14
·期 7
·3379-3390
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IF 6.1
Xingyu Lu,Hongtao Xu,Fang Fang,Juncheng Liu,Kaizhang Wu,Yuwei Zhang,Jihong Wu,Jie Gao
To investigate the prebiotic potential of two Laminaria japonica polysaccharide (LJP) fractions with different molecular weights and structures, we conducted in vitro simulated digestion and fermentation with hyperlipidemia-associated human gut microbiota. The results indicated that the LJP fraction with higher molecular weight (HLJP) appeared to have a more complex monosaccharide composition and microstructure than did the LJP fraction with lower molecular weight (LLJP), and both fractions could not be digested by in vitro simulated digestion. After in vitro fermentation, HLJP generated more short-chain fatty acids (SCFAs) and showed stronger ability to regulate core metabolites. Intriguingly, LLJP is better at promoting the proliferation of Akkermansiaceae, while HLJP is more effective in reducing the Firmicutes/Bacteroidetes ratio and increasing the content of Bacteroidaceae and Tannerellaceae. The present study indicates that LLJP and HLJP may have probiotic effects through different approaches and these differences may be related to the molecular weight and structure of the polysaccharides.
研究领域:
微生物学
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营养学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
CELL CYCLE
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2024
·
IF 3.4
Jian Yang,Xue Wang,Tao Hu,He Huang,Gang Chen,Bo Jin,Guilin Zeng,Jian Liu
Our previous findings confirmed the high enrichment of Bacteroides fragilis (BF) in fecal samples from patients with colorectal cancer (CRC). The intestinal mucosal barrier is the first defense of the organism against commensal flora and intestinal pathogens and is closely associated with the occurrence and development of CRC. Therefore, this study aimed to investigate the molecular mechanisms through which BF mediates intestinal barrier injury and CRC progression. SW480 cells and a Caco2 intestinal barrier model were treated with entero-toxigenic BF (ETBF), its enterotoxin (B. fragilis toxin, BFT), and non-toxigenic BF (NTBF). Cell counting kit-8, flow cytometry, wound healing and transwell assays were performed to analyze the proliferation, apoptosis, migration, and invasion of SW480 cells. Transmission electron microscopy, FITC-dextran, and transepithelial electrical resistance (TEER) were used to analyze damage in the Caco2 intestinal barrier model. The Azoxymethane/Dextran Sulfate Sodium (AOM/DSS) animal model was established to evaluate the effect of ETBF on intestinal barrier injury and CRC progression in vivo. ETBF and BFT enhanced the viability, wound healing ratio, invasion, and EMT of SW480 cells. In addition, ETBF and BFT disrupted the tight junctions and villus structure in the intestinal barrier model, resulting in increased permeability and reduced TEER. Similarly, the expression of intestinal barrier-related proteins (MUC2, Occludin and Zo-1) was restricted by ETBF and BFT. Interestingly, the STAT3/ZEB2 axis was activated by ETBF and BFT, and treatment with Brevilin A (a STAT3 inhibitor) or knockdown of ZEB2 limited the promotional effect of ETBF and BFT on the SW480 malignant phenotype. In vivo experiments also confirmed that ETBF colonization accelerated tumor load, carcinogenesis, and intestinal mucosal barrier damage in the colorectum of the AOM/DSS animal model, and that treatment with Brevilin A alleviated these processes. ETBF-secreted BFT accelerated intestinal barrier damage and CRC by activating the STAT3/ZEB2 axis. Our findings provide new insights and perspectives for the application of ETBF in CRC treatment.
研究领域:
胃肠病学
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肿瘤学
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微生物学
·
细胞生物学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jinghong Wei,Wangting Zhou,Wei Yi,Jiameng Shi,Yamei Yan,Jia Mi,Lu Lu,Youlong Cao,Xiaoxiong Zeng
Polysaccharides from the fruits of Lycium barbarum L. (LBPs) are the principal active constituents of L. barbarum , and it has been reported that LBPs exert regulatory effects on the gut microbiota, particularly in promoting the growth of Bacteroides . In this study, the in vitro anaerobic fermentation revealed that LBPs-4, a purified fraction of the crude LBPs, increased the relative abundances of 11 Bacteroides species, particularly B. ovatus . Results of the pure cultures indicated B. ovatus , B. thetaiotaomicron , B. fragilis and B. uniformis could effectively utilize LBPs-4 for their own growth. Transcriptome analysis of B. ovatus indicated that the degradation of LBPs-4 involved the up-regulation of three polysaccharide utilization loci (PULs). Among them, Bovatus _00022 in PUL1, which belongs to the GH28 family and is annotated as a polygalacturonase, is one of the major carbohydrate-active enzymes in the metabolism of LBPs-4. BO PG00022 was preliminarily confirmed to be effective in the degradation via the hydrolysis analysis. In conclusion, this study provided significant insights into the degradation mechanism of LBPs-4 employing gut microflora or enzymes as targets, providing a theoretical basis for the precise utilization and structural verification of complex glycans.
研究领域:
微生物学
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分子生物学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）