TALANTA
·
2025
·
IF 6.1
Hao Gong,Yang Li,Yaru Liu,Yuting Shan,Yijie Wang,Cuiping Ma,Chao Shi
Pathogenic bacteria represent a significant threat to public health and the global economy due to their high infectiousness and lethality. Consequently, the rapid and accurate identification of these bacteria remains a major challenge. Electrochemical amplification-free detection methods have shown several advantages, including rapidity, simplicity and high sensitivity. However, these methods also possess certain drawbacks, such as the inefficient solid-phase hybridization of long-chain nucleic acids on the electrode surface, it is challenging to directly detect long-chain nucleic acids. In response, we have established a novel sensor platform for efficient and rapid detection of long chain nucleic acids based on the principle of HRP signal amplification. This platform incorporates macromolecular crowding agents and electric field assistance to enhance pathogenic bacteria detection efficiency in clinical samples without amplification. The detection process begins by introducing the target into a strand displacement reaction system containing a crowding agent. The crowding effect of the crowding agent is such that the long chain target rapidly replaces the biotinylated probe. Subsequently, an electric field is applied to the surface of the screen-printed electrodes for rapid hybridization, which has been modified with a capture probe. This assay is straightforward and expeditious, reducing hybridization time from 150 min to 90 s. The sensor platform showed a detection linear range of 10 2 –10 7 CFU mL −1 with the limit of detection of 10 CFU mL −1 within 25min. This platform showcases the significant potential for on-site detection of pathogenic bacteria in clinical settings and is expected to be integrated into existing diagnostic equipment.
研究领域:
生物传感
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医学诊断
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
eScience
·
2021
·
IF 0
Mengke Peng,Li Wang,Longbin Li,Zhongyou Peng,Xiannong Tang,Ting Hu,Kai Yuan,Yiwang Chen
The development of low-cost and eco-friendly aqueous electrolytes with a wide voltage window is the key to achieving safe high energy density supercapacitors (SCs). In this work, a molecular crowding electrolyte is prepared by simulating the crowded environment in living cells. Ion transport in the molecular crowding electrolyte can be effectively improved via reducing the molecular weight of the crowding agent, polyethylene glycol (PEG). The results show that PEG with a molecular weight of 200 (PEG200) can significantly improve ionic conductivity while maintaining a wide voltage window. These advantages enable commercial activated carbon-based SCs to work at 2.5 V with high energy density, outstanding rate performance and good stability for more than 10,000 cycles. On this basis, three series of molecular crowding electrolytes using sodium perchlorate, lithium perchlorate, and sodium trifluoromethanesulfonate as salts are developed, demonstrating the versatility of PEG200 for wide-voltage aqueous electrolytes.
研究领域:
材料科学
·
能源技术
✓ 已验证
DOI
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 410
·期 2
·595-604
·
IF 3.431
Jia Man,Yang Jian,Sun Ya Kun,Bai Xi,Wu Tao,Liu Zhao Sheng,Aisa Haji Akber
We aimed to improve the imprinting effect of ionic liquid molecularly imprinted polymers (MIPs) by use of a molecular crowding agent. The ionic liquid 1-vinyl-3-ethylimidazolium tetrafluoroborate ([VEIm][BF 4 ]) was used as the functional monomer and aesculetin was used as the template molecule in a crowding environment, which was made up of a tetrahydrofuran solution of polystyrene. The ionic liquid MIPs that were prepared in the crowding environment displayed an enhanced imprinting effect. NMR peak shifts of active hydrogen of aesculetin suggested that interaction between the functional monomer and the template could be increased by the use of a crowding agent in the self-assembly process. The retention and selectivity of aesculetin were affected greatly by high molecular crowding, the amount of high molecular weight crowding agent, and the ratio of [VEIm][BF 4 ] to aesculetin. The optimal MIPs were used as solid-phase extraction sorbents to extract aesculetin from Cichorium glandulosum . A calibration curve was obtained with aesculetin concentrations from 0.0005 to 0.05 mg mL −1 (correlation coefficient R
2 of 0.9999, y = 1519 x + 0.0923). The limit of quantification was 0.12 μg mL −1 , and the limit of detection was 0.05 μg mL −1 . The absolute recovery of aesculetin was (80 ± 2)% ( n = 3), and the purity of aesculetin was (92 ± 0.5)% ( n = 5). As a conclusion, molecular crowding is an effective approach to obtain ionic liquid MIPs with high selectivity even in a polar solvent environment.
研究领域:
化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2022
·Vol. 66
·期 7
·2101088
·
IF 6.575
Hui-jian Chen,Xi-yue Yan,Ao Sun,Li Zhang,Jing Zhang,You-e Yan
Scope High-fat-diet (HFD) is an important factor in obesity. Extracellular matrix (ECM) regulates white adipose tissue (WAT), but its mechanism is unknown. Methods and Results This study uses three models—HFD-fed mice, human with obesity, and 3T3-L1 adipocytes with oleic acid (OA)/macromolecular crowders (MMC) treatment. Glucose and lipids metabolic disorders, increased collagen I/IV and laminin α2/4 (LAMA2/4), and upregulated integrins (ITGA1/ITGA7) – focal adhesion kinase (FAK) – c-Jun N-terminal kinase (JNK)/extracellular regulated protein kinase 1/2 (ERK1/2) signals in obese WAT from mice and human are observed. The upregulation of ECM – integrin – FAK signals is stronger in subcutaneous WAT than that in visceral WAT of mice, but these results are reversed in human. In vitro, oleic acid (OA) promotes lipid accumulation and upregulates collagen IV, LAMA4, and p-JNK. MMC is used to induce ECM deposition in adipocytes. MMC promotes adipocyte differentiation and integrins – FAK – JNK/ERK1/2 signals. When FAK phosphorylation is inhibited, downstream p-JNK is decreased. Inhibition of FAK phosphorylation reduces adipocyte differentiation, but MMC partially reverses this effect. Conclusion HFD-induced ECM deposition, whose signals are transmitted into adipocytes through upregulating ITGA1/ITGA7, activates the phosphorylation of intracellular FAK – JNK/ERK1/2 signals, and promotes adipogenesis in WAT. This mechanism provides novel therapeutic targets to treat obesity.
研究领域:
营养学
·
细胞生物学
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分子生物学
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代谢紊乱
·
肥胖研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
·
2022
·
IF 6.117
Hui-jian Chen,Xi-yue Yan,Ao Sun,Li Zhang,Jing Zhang,You-e Yan
Obesity and metabolic disorders are threats to human health . Extracellular matrix (ECM) is an important member of adipose microenvironment . ECM remodeling contributes to obesity and insulin resistance, but the roles of every single ECM component is still not fully understood. We observed glucose and lipids metabolic disorders in high-fat diet (HFD)-fed mice and humans with obesity. Higher levels of inflammatory factors and hormones existed in serum of HFD-fed mice. Multiple collagens , laminins , fibronectin , nidogen , and Hspg2 were upregulated in obese white adipose tissue (WAT) from mice and humans. These effects were stronger in subcutaneous WAT than visceral WAT in mice, but the fat depot difference was reversed in humans. The ECM structure and the morphology of adipocytes seeded on ECM were changed in the HFD group. In human visceral WAT, ECM genes showed positive correlations with blood lipids and glucose. In vitro , collagen I/IV and LAMA4 proteins showed similar changes with C/EBPα during the differentiation of adipocytes. Macromolecular crowders (MMC) promoted partial collagen and non-collagen gene expression. Oleic acid (OA) and MMC upregulated collagen I/IV and LAMA4 proteins, and the effects of MMC were stronger than that of OA. Moreover, MMC promoted the differentiation of adipocytes, but OA increased the size of lipid droplets . Positive correlations were observed between ECM genes and adipogenesis-related genes in adipocytes. In conclusion, some obesogens (such as HFD) induce ECM remodeling, and the upregulation of ECM components is closely related to adipogenesis , suggesting that adipose ECM deposition is an indicator of obesity and metabolic disorders.
研究领域:
内分泌学
·
代谢学
·
细胞生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Wan-Yi Ge,Wen-Pu Shi,Xue-Ting Wang,Huan Liang,Xu-Dong Deng,Liang-Liang Chen,Xiao-Qian Jin,Wei-Hong Guo,Da-Chuan Yin
Protein liquid–liquid phase separation (LLPS) is a significant process in biology and material sciences. Finding suitable conditions for protein LLPS is essential for its in-depth and systematic study. Here, we provide a mixed solution screening kit based on biomacromolecule crowding agents to find protein LLPS conditions. The screening kit consists of 96 chemical formulations, in which the biomacromolecule crowding agents are used as the key components. The aqueous solution of the target protein is mixed with each chemical agent in the kit and then placed in an incubator for some time. The LLPS conditions for the target protein can be found by examining the LLPS phenomena in the droplets. We used nine proteins as model target proteins—lysozyme, bovine serum albumin, bovine hemoglobin, ovalbumin, catalase, FUS, Aβ, Tau, and α-Synuclein—to evaluate the performance of the screening kit. We found that utilization of the screening kit is efficient and useful to find different conditions for LLPS of proteins. The screening results reveal two types of conditions: those that promote and those that inhibit LLPS. Both conditions are extremely valuable because those that promote LLPS can be used to do extensive studies on the LLPS processes of the proteins of interest, whereas those that inhibit LLPS can be utilized as leads for developing novel medications to treat LLPS-related disorders. Furthermore, novel applications for the kit were investigated by utilizing the screening products.
研究领域:
生物学
·
材料科学
·
生物物理学
·
药学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）