ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yan Gao,Junpeng Shi,Chen Wu,Longlong Cao,Lin Liu,Jinyuan Wang,Xiaofang Luo,Xiao Zhang,Yun Zhang
Immunochromatography is a commonly used immediate detection technique, using signal labels to generate detection signals for rapid medical diagnosis. However, its detection sensitivity is affected by background fluorescence caused by the excitation light source. We have developed an immunochromatographic test strip using Zn2GeO4:Mn2+ (ZGM) persistent luminescent nanoparticles (PLNPs) for immediate fluorescence detection and highly sensitive persistent luminescence (PersL) detection without background fluorescence interference. ZGM emits a strong green light when exposed to ultraviolet (UV) excitation, and its green PersL can persist for over 30 min after the excitation light is turned off. We modified the surface of ZGM with heparin-binding protein (HBP) antibodies to create immunochromatographic test strips for the detection of HBP as the target analyte. Under UV excitation, the chromatography test paper can be visually observed at concentrations as low as 25 ng/mL. After the excitation light source is switched off, PersL can achieve a detection limit of 4.7 ng/mL without background interference. This dual-mode immunochromatographic detection, based on ZGM, shows great potential for in vitro diagnostic applications.
研究领域:
生物医学工程
·
诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2025
·
IF 5.1
Qingqing Xie,Ailijiang Tuerdi,Xiangkai Qiao,Yalin Zheng,Aikelaimu Aihemaiti,Peng Yan,Abdukader Abdukayum
Near-infrared persistent luminescent nanoparticles (PLNPs) are widely used for deep tissue penetration in bio-imaging. Ga2O3:Cr3+, a promising phosphor material, has been extensively studied for its suitable band gap and tunable emission wavelength. Although a redshift in the fluorescence wavelength has been achieved, the persistent luminescence properties of Ga2O3:Cr3+ following this redshift have not been extensively explored. Herein, we have prepared Ga1.99−xInxO3:0.01Cr3+ (x = 0–1.99)(GIO) PLNPs utilizing a hydrothermal method followed by calcination. Replacing gallium with indium in the crystal structure of Ga2O3:Cr3+ redshifts the emission wavelength to 830 nm and induces persistent luminescence, which is attributed to lattice distortion caused by the substitution of gallium with indium, which modifies the crystal field around the Cr3+ ions. The as-prepared GIO nanoparticles show potential for future low-cost, deep-tissue bioimaging applications.
研究领域:
材料科学
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生物医学工程
·
光学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Xiang Chen,Haiyang Zhang,Xiaoye Liang,Lingling Li
Glutathione (GSH) is one of the important substances to maintain the redox balance in vivo, accurate monitoring of its changes is conducive to the early diagnosis and treatment of related diseases. Herein, we reported a two-in-one nanoflower-structured sensing platform (ZCGO@MnO 2 ) with luminescence resonance energy transfer (LRET) property and oxidase-like activity for dual-mode detection of GSH. The sensor consisted of persistent luminescence nanoparticles (PLNPs) with near-infrared emission and manganese dioxide (MnO 2 ) with luminescence quenching ability and oxidase-like activity. The addition of GSH could reduce MnO 2 to Mn 2+ , resulting in the recovery of persistent luminescence and inhibition of oxidase-like activity, then achieving persistent luminescence and colorimetric dual-mode signal outputs. The sensor utilized the persistent luminescence of PLNPs to eliminate the background interference of the complex biological matrix, improved the signal-to-noise ratio (S/N) of detection and took advantage of dual-mode sensing to improve the accuracy of detection, showing excellent analytical performance. Besides, the sensor was capable of monitoring changes in intracellular GSH levels through bioimaging . This work provides new ideas for the design of sensors in complex samples and broadens the application of PLNPs in the field of biosensing.
研究领域:
分析化学
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生物医学工程
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纳米科技
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Zichao Yan,Yifei Wang,Minghan Qiu,Kai Long,Zhouyu Zhang,Mengjie Sun,Chang Yin,Wei Wang,Huaqing Wang,Zhi Yuan
Surgical resection remains the main treatment for malignant tumors. Image-guided surgery aims to remove tumor tissue completely while preserving normal tissue, thereby reducing patient recurrence rates and injury. However, challenges like tissue autofluorescence, limited probe penetration and low contrast restrict its use. Near-infrared (NIR) persistent luminescent nanoparticles (PLNPs) provide a solution by emitting persistent luminescence (PersL) even after excitation ceases, thus circumventing autofluorescence and enabling deep tumor imaging. In this study, we prepared nano-sized (140-nm hydrodynamic size) Cr3+ doped zinc gallogermanate (ZGC) using a removable template method and modified it with folate acid to obtain ZGC-FA, which exhibits NIR (695 nm) PersL with a signal-to-noise ratio of 23.9 in vivo. We utilized colon cancer model that selectively expressing luciferase for the first time to validate the guiding efficacy of ZGC-FA in the precision surgical resection. Post-intraperitoneal injection at 50 minutes, the PersL closely matched the tumor boundaries, achieving an overlap rate of approximately 98%. Complete tumor resection was achieved under PersL guidance, with only 2.3% of healthy tissue removed. This research underscores the potential of ZGC-FA in the field of surgical oncology. The precision of the ZGC-FA guided surgical approach holds promise to enhance surgical outcomes and facilitate postoperative recovery for patients.
研究领域:
肿瘤学
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医学影像
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生物材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Zi-Jin Wei,Kai Long,Chang Yin,Xinxin Yuan,Mengjie Sun,Wei Wang,Zhi Yuan
ZnGa2O4:Cr3+ (ZGC) persistent luminescence (PersL) nanoparticles (PLNPs) are extensively researched in the fields of bioimaging and therapy due to their simple preparation and uniform morphology. Typically, the luminescence intensity increases with luminescent ions Cr3+ concentration up to an optimal range of 0.4%-0.6%, beyond which concentration quenching often occurs, leading to a decrease in both intensity and lifetime. In this study, we introduce a one-pot solvothermal synthesis address the issue of concentration quenching by coating ZGCn PLNPs with an optimal concentration of the organic dye OAm-RhB, yielding ZGCn@OAm-RhB PLNPs. Here, OAm-RhB serves as a strong absorber, harvesting energy and subsequent energy transfer to Cr3+. Notably, the Cr3+ doping concentration can be increased to 1.2% without causing concentration quenching. Strikingly, the energy transfer efficiency between OAm-RhB and Cr3+ is up to 71%. ZGC1.2@OAm-RhB PLNPs significantly enhance the afterglow intensity by 60 times and extend the lifetime from 85.35 to 112.05 s. After modification with DSPE-PEG and HA, ZGC1.2@OAm-RhB@PEG@HA PLNPs were obtained, demonstrating good cellular uptake and precise tumor imaging, guiding complete tumor resection confirmed by H & E staining analysis. This strategy is anticipated to mitigate concentration quenching of other PLNPs for improving PersL performance.
研究领域:
材料科学
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生物医学工程
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光学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2017
·Vol. 9
·期 20
·6846-6853
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IF 7.367
Zhihao Zhou,Wei Zheng,Jintao Kong,Yan Liu,Ping Huang,Shanyong Zhou,Zhuo Chen,Jianlin Shi,Xueyuan Chen
Persistent luminescence nanoparticles (PLNPs) have shown great promise in the field of biomedicine, but are currently limited by the challenge in the synthesis of high-quality PLNPs with bright persistent luminescence and a long afterglow time. Herein, we report a facile strategy for the synthesis of monodisperse, rechargeable and LED-activated ZnGa2O4 : Cr3+ near-infrared (NIR) PLNPs based on a modified solvothermal liquid–solid-solution method. The as-synthesized PLNPs are not only flexible for bioconjugation, but could also circumvent the limitation of the weak persistent luminescence and short afterglow time that most PLNPs confronted owing to their rechargeable capability. It was unraveled that both thermal activation and quantum tunneling mechanisms contributed to the afterglow decay of the PLNPs, and the quantum tunneling was found to dictate the LED-activated afterglow intensity and lasting time. Furthermore, by utilizing the superior excitation-free persistent luminescence, we demonstrated for the first time the application of biotinylated ZnGa2O4 : Cr3+ PLNPs as background-free luminescent nano-bioprobes for sensitive and specific detection of avidin in a heterogeneous assay with a limit of detection down to ∼150 pM, thus revealing the great potential of these NIR PLNPs in ultrasensitive biodetection and bioimaging.
研究领域:
材料科学
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生物医学工程
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
Nanoscale
·
2016
·Vol. 8
·期 45
·18987-18994
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IF 7.76
Huai-Xin Zhao,Cheng-Xiong Yang,Xiu-Ping Yan
Persistent luminescent nanoparticles (PLNPs) show great potential in realizing precision imaging due to the absence of in situ excitation and no background interference. However, the current PLNP-based tumour imaging is usually achieved by single targeting or passive targeting strategies, and thus it lacks high specificity and affinity for efficient persistent luminescence imaging in vivo. Herein we report the bioconjugation of multiple targeting ligands on the surface of PLNPs for dual-targeted bioimaging to improve the specificity and affinity of the PLNP nanoprobe for in vitro and in vivo bioimaging. The PLNPs were prepared by co-doping CrIII and BIII into ZnGa2O4via a hydrothermal–calcination method. While CrIII doped ZnGa2O4 PLNPs possess excellent near-infrared luminescence along with long afterglow and red light renewable near-infrared luminescence, doping of BIII into the PLNPs further improves the persistent luminescence. Conjugation of two targeting ligands, hyaluronic acid and folic acid, which have specificity toward the cluster determinant 44 receptor and folic acid receptor in tumour cells, respectively, provides synergistic targeting effects to enhance the specificity and affinity toward tumour cells. This work provides a dual-targeting strategy for fabricating PLNP-based nanoprobes to realize precision tumour-targeted bioimaging.
研究领域:
材料科学
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生物医学工程
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癌症研究
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 27
·6723-6731
·
IF 4.3
Dai Wenjing,Qi Bing,Li Zhihao,Wang Jie
In optical biosensing, analyte-independent factors such as autofluorescence interference and excitation source fluctuation decrease the sensitivity and accuracy. Herein, we reported a bimodal persistent luminescence strategy to design dual-emissive persistent luminescence nanoparticles (PLNPs) with built-in self-calibration to preclude interference from analyte-independent factors in biosensing. As a proof of concept, ZnGa 2 O 4 :Cr PLNPs with emissions at both 490 nm and 695 nm were designed. The I 490 /I 695 ratio of ZnGa 2 O 4 :Cr was readily adjusted by simply changing the doping concentration of Cr 3+ . The ZnGa 2 O 4 :Cr PLNPs were employed for the ratiometric detection of urinary mesna. A good linear relationship between the I 490 /I 695 ratio of ZnGa 2 O 4 :Cr-based nanoprobe and the concentration of mesna was obtained in the range of 0–40 μM. The limit of detection was about 0.40 μM. Results showed that autofluorescence interference from urine was totally eliminated by collecting the persistent luminescence signal of ZnGa 2 O 4 :Cr after excitation ceased. Moreover, the built-in self-calibration feature of the ratiometric ZnGa 2 O 4 :Cr PLNPs efficiently suppressed the interference from fluctuations in instrumental parameters during urinary mesna detection. The recovery rates of mesna in the spiked urine samples are in the range of 99.1~109.0%, showing the reliability of the ratiometric ZnGa 2 O 4 :Cr PLNPs in urinary mesna detection. ZnGa 2 O 4 :Cr can further be expanded to the detection of other analytes in complex matrices. This study may open new opportunities for the design of dual-emissive PLNPs with tunable ratios of emission intensity, and it can further promote the applications of optical biosensing in disease diagnosis, food safety, and environmental monitoring. Graphical abstract
研究领域:
生物传感
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光学检测
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医学诊断
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2024
·
IF 3.2
Xiu-Zhen Yang,Tian-Qi Zhao,Renagul Abdurahman,Shi-Ji Liu,Yao-Xing Han,Yue Zhang,Xue-Bo Yin,Jie Gao
Fluorescence intensity ratio (FIR) technique demonstrates significant potential for non-contact temperature sensing with rapid response time, while the design of FIR probes is particularly intriguing. Herein, we report Pr–Al co-doped MgGa 2 O 4 persistent luminescence nanoparticles (PLNPs), as MgAl 0.04 Ga 1.96 O 4 : 0.1 % Pr 3+ (MAGP), for ratiometric temperature sensing application. Pr 3+ ions function as the emission centers for dual-emission at 612 and 494 nm, while Al 3+ ions adjust the lattice environment around Pr 3+ ions to enhance the luminescence. Consequently, 274 nm excitation induces the emissions at 494 and 612 nm, as the transitions of Pr 3+ for 3 P 0 → 3 H 4 and 1 D 2 → 3 H 4 , respectively. The emission mechanism is substantiated by density functional theory calculations, regarding the electronic transitions of Pr 3+ . Ratiometric temperature sensing is achieved over the temperature range of 303–573 K with the thermally non-coupled mechanism of the 3 P 0 / 1 D 2 energy levels by FIR at 612 and 494 nm, respectively. The maximum relative sensitivity was 0.91 %. The mechanism is corroborated as the non-thermally coupled energy levels through intervalence charge transfer. Thus, the reversible and sensitive response was illustrated for temperature measurement and the non-thermally coupled mechanism is efficient for the design of ratiometric temperature sensors.
研究领域:
材料科学
·
光学工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 10
·1479-1488
·
IF 4.776
Dong-Dong Zhang,Jing-Min Liu,Nan Song,Yao-Yao Liu,Meng Dang,Guo-Zhen Fang,Shuo Wang
Infection by pathogens has always been a major threat to human health, and various drugs have been explored and designed to kill pathogens in the past decades. However, pathogens are evolving faster than the development of new antibiotics, and increasing doses are resulting in increasing side effects and toxicity; this has prompted us to exert effort toward the development of advanced drug carriers for precise delivery of antibiotics. In this study, with the involvement of persistent luminescence nanophosphors (PLNPs) as the emission core, we propose an antibiotic nanocarrier for in vivo delivery of vancomycin to intestinal bacteria via bioimaging guidance. The synthesized PLNPs were coated with mesoporous silica for vancomycin adsorption (NPs@SiO2@Van) and used as an efficient nanocarrier for direct vancomycin delivery and in vivo imaging with low cytotoxicity toward MC38 cell lines. Additionally, we detected the luminescence signals of NPs@SiO2@Van during their use as nanocarriers for vancomycin and accurately obtained the biodistribution of NPs@SiO2@Van in situ and in real time with neglectable auto-fluorescence from the animal body. For the first time, bioimaging-guided in vivo drug delivery to gut bacteria was realized in the present work. The outstanding luminescence features and excellent biocompatibility and structural stability of PLNPs may open new horizons in the development of nanocarriers for nano-diagnosis/therapy and in vivo studies of intestinal microorganisms.
研究领域:
生物医学工程
·
纳米医学
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
ACS Nano
·
2021
·
IF 15.881
Jun Wang,Qizhen Li,Hui Zhao,Wentong Yue,Kaiwen Zhang,Xingyu Jiang,Kai Li
To date, the strategic exploration of a synthetic approach to afford persistent luminescent nanoparticles (PLNPs) integrated with precisely controlled size/monodispersity and renal-clearable capability remains extremely challenging. Herein, we report a facile synthetic process with an elucidated mechanism to fine-tune the size for acquiring renal-clearable PLNPs, using mesoporous silica nanoparticles (MSNs) as a template. This strategy relies on the controlled crystallization of the precursor ions in the pore channels of MSNs at a high temperature, leading to the formation of monodispersed PLNPs with an average diameter as small as 2.5 nm after complete removal of MSN templates. The as-prepared ultrasmall PLNPs coated with polyethylene glycol exhibit uniform size, excellent water-dispersibility, good persistent luminescence, and high T1 relaxivity (17.6 mM–1·S–1), ensuring their suitability for afterglow/magnetic resonance dual-modality imaging and subsequent in vivo renal clearance. Thus, our study provides a strategy to inspire the controlled synthesis of diverse PLNPs by using MSN templates, simultaneously addressing the critical issues of precise adjustment of size and body clearance for versatile biomedical applications.
研究领域:
生物医学工程
·
材料科学
·
影像医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2018
·
IF 2.732
Xia Sun,Junpeng Shi,Shenghui Zheng,Jinlei Li,Shougang Wang,Hongwu Zhang
Inflammation is implicated in many human diseases, thus the diagnosis of inflammation is very important for the early treatment of diseases associated with inflammation. However, the high-sensitivity diagnosis of inflammation remains difficult. Near-infrared (NIR) persistent luminescence nanoparticles (PLNPs) were considered one of the most promising candidates for high-sensitivity bioimaging due to being free of autofluorescence. In this study, we synthesized PLNPs Zn 1.1 Ga 1.8 Ge 0.1 O 4 :Cr 3+ (ZGG) which demonstrated low cytotoxicity, excellent NIR persistent luminescence. We conducted the visualization of inflammation using these nanoparticles. The results indicated that the surface states of the ZGG influenced the progression of macrophage phagocytosis in vitro . ZGG-NH 2 demonstrated higher levels of internalization compared with ZGG-OH and ZGG-PEG. However, in contrast to the in vitro results, ZGG-PEG exhibited a greater ability to facilitate the visualization of inflammation in vivo . The long chains of PEG on the ZGG-PEG surfaces resulted in a lower reticuloendothelial system capture rate compared with both ZGG-OH and ZGG-NH 2 . A higher concentration of the ZGG-PEG would cycle through the inflammation site, thereby inducing a high level of ZGG-PEG retention and thus yielding the strongest luminescent signal. Taken together, the results of this study demonstrate a simple and novel method for high-sensitivity visualization of inflammation in vivo .
研究领域:
生物医学工程
·
分子影像学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）