Biomaterials Science
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2025
·
IF 5.7
Min Sun,Jinke Cao,Yang Zou,Haiyan Ju,Yonggang Lv
During tendon injury repair, deficiency of basic fibroblast growth factor (bFGF) is a critical factor leading to unsatisfactory repair results. This study aims to prepare bFGF-loaded zeolite imidazole framework-8 (ZIF-8) nanocrystals using one-pot synthesis method. Subsequently, a bilayer nanofibrous membrane incorporating these drug-loaded nanocrystals was fabricated through electrospinning technology. The potential of this composite nanofibrous membrane to facilitate the continuous release of bFGF at the site of tendon injury was evaluated, with the aim of enhancing the quality of tendon repair. The efficacy of the nanofibrous membrane in promoting tendon differentiation, preventing tendon adhesion, and facilitating tendon repair was assessed through both in vitro and in vivo experiments. At the site of tendon injury, the degradation of ZIF-8 in an acidic microenvironment resulted in the release of bFGF and Zn2+, which contribute to the enhancement of tendon repair. ZIF-8 nanocrystals, achieving an encapsulation efficiency of 50.13% ± 1.42%. Following a continuous release period exceeding 40 days, the cumulative in vitro release rate was determined to be 35.02% ± 4.27%. The incorporation of ZIF-8 nanocrystals into a nanofibrous membrane demonstrated the ability to effectively preserve the bioactivity of bFGF while enabling sustained sustained release at the site of tendon injury, thereby facilitating tendon repair. The findings offer novel insights into the treatment of tendon injuries and provide significant theoretical guidance for the tendon repair process.
研究领域:
生物医学工程
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组织工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xiaoyue Li,Yanai Chen,Huiyan Shao,Zheng Yan,Yun Wang,Shenzhou Lu
As a new type of transdermal drug delivery preparation, microneedles can improve the bioavailability of drugs and enhance patient compliance. This article develops a melatonin transdermal sustained-release microneedles using silk fibroin as the substrate. The silk fibroin in microneedles forms a SILK I crystal structure, which does not dissolve in the skin but has certain swelling properties. Microneedles have superior mechanical properties, and can puncture the skin to form a sustained-release channel in the dry state. After drug release is completed, wet microneedles can be pulled out. The results of in vitro transdermal experiments show that the effective drug release time of microneedles is significantly correlated with drug loading. The sustained release time of melatonin silk fibroin microneedles with a drug loading of 1 mg/tablet reach 11 h, with a cumulative release rate of over 85 %. The results of in vivo animal experiments show that silk fibroin melatonin microneedles can maintain a stable blood drug concentration for up to 8 h. The results of in vivo pharmacodynamics show that melatonin microneedles have significant therapeutic effects on insomnia model rats, improving disrupted circadian rhythms and alleviating anxiety.
研究领域:
药剂学
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生物医学工程
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2015
·Vol. 4
·期 4
·760-767
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IF 4.726
Liping wu,Liyan Qiu
Combretastatin A4 phosphate (CA4P), the water-soluble phosphate pro-drug of combretastatin A4 (CA4), acts as a novel vascular disrupting agent (VDA). It strongly inhibits tubulin polymerization and selectively exerts action against tumor blood vessels. However, its quite short half-life (∼30 min) results in a short action time and consequently limits its application. Therefore, it is beneficial to develop a sustained-release preparation of CA4P for relatively long action duration. On the other hand, conventional PLGA microspheres display poor encapsulation capability for water-soluble small molecules due to rapid diffusion outside. Hence, we developed CA4P-loaded microspheres (CA4P-MS) composed of methoxy poly(ethylene glycol)-b-polylactide (PELA) reverse micelles and PLGA with a nano-in-micro sea-island structure. The CA4P encapsulation efficiency of CA4P-MS reached 92.88%. More interestingly, sustained drug release was achieved, which contributed to the prolonged inhibition effect on S180 tumor during a 30-day period with one-time subcutaneous administration. In conclusion, our study established a biodegradable polymer based platform to construct microspheres for water-soluble small molecular compounds with sustained release and a long-lasting action.
研究领域:
药剂学
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肿瘤学
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生物材料
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产品关联
DOI
PubMed
高影响因子
CRYSTAL GROWTH & DESIGN
·
2020
·
IF 4.089
Chunyang Guo,Qi Zhang,Bingqing Zhu,Zaiyong Zhang,Junjie Bao,Qiaoce Ding,Guobin Ren,Xuefeng Mei
Nicorandil (NCD) is a chemically unstable drug and sensitive to humidity, heat, and mechanical stress in manufacturing processes. In this work, the cocrystals of NCD with organic acids were designed and synthesized to enhance the stability of NCD. Cocrystals of NCD with 1-hydroxy-2-naphthoic acid, salicylic acid, 3-hydroxybenzoic acid, and 2,5-dihydroxybenzoic acid were obtained. Hydrothermal stability study showed that cocrystals performed with remarkable stability advantages compared with pure NCD. On the basis of mechanism research, the stability improvements of NCD molecules can be attributed to the realignments of NCD molecules in cocrystals. After the formation of cocrystals, the novel packing styles of NCD break the self-catalyzed decomposition process in pure NCD structure. Moreover, intrinsic dissolution rate characterizations showed that the formation of cocrystals could also optimize the dissolution behavior of NCD to realize the objective of sustained release.
研究领域:
药学
·
材料科学
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DOI
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Wenzuo Luo,Xingui Zhang,Yingying Chu,Tiantian Chen,Huixuan Sun,Jiadong Liu,Guoxing Zhang,Lihong Fan,Haixing Xu,Yue Zhang,Ming Yang
Although finasteride (FNS) tablets are considered the most effective drug for the treatment of androgenetic alopecia (AGA), their clinical applications are limited due to the associated side effects including decreased libido, breast enlargement, and liver dysfunction. In this study, we have developed a personalized microneedle (PMN) with a double-layer structure that incorporates FNS-loaded microspheres (MPs) to accommodate irregular skin surfaces. This design enables the sustained release of FNS, thereby reducing potential side effects. The needle body was synthesized with high-strength hyaluronic acid (HA) as the base material substrate. The backing layer utilized methacrylate gelatin (GelMA) with specific toughness, enabling PMN to penetrate the skin while adapting to various skin environments. The length of PMN needles (10 × 10) was approximately 600 μm, with the bottom of the needles measuring about 330 μm × 330 μm. The distance between adjacent tips was around 600 μm, allowing the drug to penetrate the stratum corneum of the skin. The results of the drug release investigation indicated the sustained and regulated release of FNS from PMN, as compared to that of pure FNS and FNS-MPs. Further, the cytotoxicity assay demonstrates that PMS displays good cytocompatibility. Altogether, this mode of administration has immense potential for the development of delivery of other drugs, as well as in the medical field.
CAS / MDL:
研究领域:
药剂学
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皮肤科学
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生物材料学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2022
·
IF 4.855
Fangwei Qi,Zhihui Wang,Yang Shuai,Shuping Peng,Cijun Shuai
Strontium ion (Sr2+) enabled us to endow polymer bone scaffolds with bioactivity owing to its excellent osteoinductive capacity. Nevertheless, the burst release of Sr2+ hindered its further application. Herein, strontium substituted hydroxyapatite (SrHA) was constructed to achieve a sustained release system. Specifically, Sr2+ could substitute Ca2+ in a HA lattice framework through ion exchange due to their similar ion radius. The ionic bond formed by Sr2+ and surrounding ions could prevent the rapid escape of Sr2+ and thereby achieve the sustained release of Sr2+. Subsequently, SrHA was introduced into the poly-l-lactic (PLLA) scaffold fabricated by selective laser sintering. Results demonstrated that PLLA/SrHA scaffolds presented a sustained Sr2+ release with a cumulative concentration of 5.6 mg/L over 28 days. The released Sr2+ significantly promoted the cell proliferation and differentiation. Furthermore, the tensile and compressive strengths of PLLA/SrHA scaffolds were also greatly enhanced in comparison with that of PLLA scaffolds. These positive findings demonstrated that PLLA/SrHA scaffolds had considerable potential for bone repair.
研究领域:
生物材料学
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骨组织工程
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医学
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产品关联
DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 9
·1669
·
IF 3.06
Yidi Zhang,Chong Wang,Li Fu,Shan Ye,Min Wang,Yanmin Zhou
Osteogenic peptides have been reported as highly effective in directing mesenchymal stem cell osteogenic differentiation in vitro and bone formation in vivo. Therefore, developing novel biomaterials for the controlled delivery of osteogenic peptides in scaffolds without lowering the peptide’s biological activity is highly desirable. To repair a critical-sized bone defect to efficiently achieve personalized bone regeneration, a novel bioactive poly(lactic-co-glycolic acid) (PLGA)/β-tricalcium phosphate (β-TCP) composite scaffold, in which graphene oxide (GO) and bone morphogenetic protein (BMP)-2-like peptide were loaded in situ (PTG/P), was produced by an original cryogenic 3D printing method. The scaffolds were mechanically comparable to human cancellous bone and hierarchically porous. The incorporation of GO further improved the scaffold wettability and mechanical strength. The in situ loaded peptides retained a high level of biological activity for an extended time, and the loading of GO in the scaffold further tuned the peptide release so that it was more sustained. Our in vitro study showed that the PTG/P scaffold promoted rat bone marrow-derived mesenchymal stem cell ingrowth into the scaffold and enhanced osteogenic differentiation. Moreover, the in vivo study indicated that the novel PTG/P scaffold with sustained delivery of the peptide could significantly promote bone regeneration in a critical bone defect. Thus, the novel bioactive PTG/P scaffold with a customized shape, improved mechanical strength, sustainable peptide delivery, and excellent osteogenic ability has great potential in bone tissue regeneration.Keywords:osteogenic peptide;sustained delivery;bone tissue engineering;scaffolds;graphene oxide
研究领域:
生物医学工程
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材料科学
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组织工程
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产品关联
DOI
PubMed
高影响因子
Drug Design Development and Therapy
·
2019
·
IF 3.208
Hong Huang,Houhe Liu,Hua Zhou,Zhiling Liang,Dandan Song,Yun Zhang,Wanqiu Huang,Xiaotian Zhao,Bo Wu,Guodong Ye,Yugang Huang
Background Sucrose allyl ether (SAE) containing hemostatic drugs and a photoinitiator was established to treat mild postpartum hemorrhage or long-term continuous abnormal uterine bleeding in minimally invasive surgery (MIS) using a photopolymerization method.Methods and results Real-time infrared spectroscopy and rheological experiments showed that the SAE monomer with shear-thinning characteristics could polymerize rapidly into a transparent membrane. Cytotoxicity experiments in vitro showed that this system could elicit a long-term hemostatic effect. Tissue adhesion was also evaluated. The photo-stability of four delivered antifibrinolytic drugs (6-aminocaproic acid, ethylenediaminediacetic acid, tranexamic acid and p-(aminomethyl) benzoic acid) was tested by ultraviolet-photolysis experiments and illustrated by time-dependent density functional theory. Sustained-release experiments revealed that the formed film could be used as a drug carrier. Molecular docking and molecular dynamics were done to investigate the binding mechanism between hemostatic drugs as ligands and the human plasminogen kringle-1 (1HPK) as a target.Conclusion It has been suggested that SAE with tranexamic acid could be a drug-release system of microchannel transport used in MIS. This system could tackle the dilemma of fluidity and adhesion in MIS. The photo-stable tranexamic acid was the most suitable drug according to its satisfactory binding energy, good photo-stability, and sustained release.
研究领域:
医学
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药学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2015
·
IF 3.65
Li Qingcong,Pan Xiaoxia,Li Hongli,Yuan Minglong
A relatively high molecular weight of 4-arm star PELA was obtained by ring-opening polymerization of l -lactic acid O -carboxyanhydride with 4-arm-PEG in the presence of DMAP as an initiator. The results via 1 H NMR and 13 C NMR show that the end of the star PELA chain is a hydroxyl group and the central core is a PEG group. Rotavirus (strain SA11) was incorporated into 4-arm star PELA microspheres formulated by the water in oil in water emulsification solvent extraction method. The microspheres produced were spherical, and the mean diameter was 1.34 μm with a narrow size distribution. The drug release profile displayed a low burst release effect of 1.8% on the first day and a sustained release of antigen over 100 days. After the immunization of mice, the microsphere-entrapped RV elicited improved and long-lasting IgA and IgG antibody response in serum detected by ELISA in comparison to the free RV antigen. This study shows that 4-arm-PEG is an effective initiator for the ring-opening polymerization of Lac-OCA by DMAP as an initiator and that the resulting polymer is useful as a delivery system for the rotavirus vaccine .
研究领域:
生物材料学
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疫苗学
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药剂学
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产品关联
DOI
PubMed
高影响因子
DRUG DELIVERY
·
2022
·
IF 6.819
Yujin Zhao,Chang Huang,Zhutian Zhang,Jiaxu Hong,Jianjiang Xu,Xinghuai Sun,Jianguo Sun
Glaucoma is the leading cause of irreversible vision loss worldwide, and reduction of intraocular pressure (IOP) is the only factor that can be interfered to delay disease progression. As the first line and preferred method to treat glaucoma, eye drops have many shortcomings, such as low bioavailability, poor patient compliance, and unsustainable therapeutic effect. In this study, a highly efficient brimonidine (BRI) silicone rubber implant (BRI@SR@TPU implant) has been designed, prepared, characterized, and administrated for sustained relief of IOP to treat glaucoma. The in vitro BRI release from BRI@SR@TPU implants shows a sustainable release profile for up to 35 d, with decreased burst release and increased immediate drug concentration. The carrier materials are not cytotoxic to human corneal epithelial cells and conjunctival epithelial cells, and show good biocompatibility, which can be safely administrated into rabbit’s conjunctival sac. The BRI@SR@TPU implant sustainably released BRI and effectively reduced IOP for 18 d (72 times) compared to the commercial BRI eye drops (6 h). The BRI@SR@TPU implant is thus a promising noninvasive platform product for long-term IOP-reducing in patients with glaucoma and ocular hypertension.
研究领域:
药剂学
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眼科医学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 26
·15762-15768
·
IF 3.108
Zideng Gao,Long Pang,Haojie Feng,Shunyi Wang,Qiuyun Wang,Mengyao Wang,Yining Xia,Shuwen Hu
Encapsulation of pesticides with polymeric materials is a promising approach to improve the efficacy of pesticide application and reduce the potential risks of pesticides to the environment and humans. In this study, a novel imidacloprid microcapsule was prepared by depositing a polydopamine (PDA) layer on imidacloprid via oxidative self-polymerization followed by a polyurea (PU) layer on PDA via copolymerization. No organic solvent or surfactant was used in the preparation process. The entrapment rate of imidacloprid was determined to be 47.28 ± 0.87%, and the pesticide loading of microcapsule was 68.36 ± 1.13%. Several characterization assays, such as Fourier infrared transform analysis, thermogravimetric analysis and contact angle analysis, were conducted to confirm the encapsulation of imidacloprid. Dynamic light scattering showed that the microcapsule size ranged from hundreds of nm to several μm, which was similar to the results obtained by scanning electron microscopy. The microcapsule had a stable dispersion up to one month in the aqueous solution. Moreover, the microcapsule was able to release imidacloprid in a sustainable manner, as the release was much slower than the non-encapsulated imidacloprid.
研究领域:
农业化学
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材料科学
·
环境科学
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DOI
高影响因子
Environmental Science-Nano
·
2023
·Vol. 11
·期 1
·363-372
·
IF 7.3
Long Chen,Jiamin Hu,Haiwei Pang,Shuyan Yin,Huixiang Liu,Yehan Tian,Shangkun Gao,Chenggang Zhou,Puxia Wu,Shuo Miao,Yingchao Ji,Chaoqiong Liang,Yanxue Liu
Pine wilt disease (PWD) is an infestation caused by pine wood nematodes (PWN, Bursaphelenchus xylophilus), and has caused significant disruption to forest ecosystems worldwide. Trunk injection is effective in controlling PWD, but the long-term use of abamectin (AVM) and other drugs in large quantities has caused resistance problems, and the annual trunk injections have caused some damage to the trunks themselves. In order to reduce drug resistance and damage to the tree trunks, in this study, dendritic mesoporous silica nanoparticles (DMSNs) were prepared by using a “one-pot” method, which is the easiest to industrialise, and AVM@DMSNs nano-pesticides with a uniform particle size, high loading efficiency (80.2%) and sustained release were prepared by physical adsorption. And cellular uptake and toxicity experiments were carried out on sf9 cells, and the results showed that the nano DMSNs could be enriched on sf9 cells and had good inhibition of cellular activity. The lethality of the nano-pesticides AVM@DMSNs and AVM on PWN was investigated using the insect dip method. The results showed that the corrected mortality rate of AVM@DMSNs was significantly higher than that of free AVM within 72 h. In addition, AVM@DMSNs compounded with a plant essential oil bark penetrant could be applied locally outside the bark and penetrate the drug into the tree to achieve the purpose of injury-free drug delivery, which could effectively reduce the damage of perforation injection on the tree and provide a new way for the control of forest pests.
研究领域:
植物保护
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森林生态学
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纳米生物学
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DOI
高影响因子
危险品化学品经营许可证（带存储）