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Biomedical Optics Express · 2024 ·Vol. 15 ·期 10 ·5803-5817 · IF 2.9

Microfluidic point-of-care testing device for multiplexed detection of liver function blood markers

Yingchun Li,Yingchun Li,Haobo Cheng

In this work, we developed a novel microfluidic paper-based analytical device to quantify the blood markers of liver function from human fingertips and whole blood samples. The device can quickly acquire information for screening liver injury and supporting clinical decision-making by simultaneously performing quantitative tests for alanine aminotransferase, aspartate aminotransferase, and albumin. We evaluated the detection accuracy and the storage stability of the device using fingertip samples. The yielded results of our device correlated well with those from Mindray BS350s, even under the conditions of 35 °C and 90%RH. Thus, it offers an effective platform for clinical assessment of liver injury particularly in resource-limited areas.

CAS / MDL: 328-50-7

研究领域: 医学诊断 · 生物工程

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ACS Sensors · 2023 · IF 8.9

Fast and Sensitive Detection of Protein Markers Using an All-Printing Photonic Crystal Microarray via Fingertip Blood

Jimei Chi,Meng Su,Bingjie Xue,Lijun Cheng,Zewei Lian,Yang Yun,Xu Yang,Xue Wang,Hongfei Xie,Huadong Wang,Yuan Wang,Jie Du,Yanlin Song

With the demand for point-of-care testing (POCT) in cardiovascular diseases, the detection of biomarkers in trace blood samples is of great significance in emergency medicine settings. Here, we demonstrated an all-printed photonic crystal microarray for POCT of protein markers (named “P4 microarray”). The paired nanobodies were printed as probes to target the soluble suppression of tumorigenicity 2 (sST2) as a certified cardiovascular protein marker. Benefiting from photonic crystal-enhanced fluorescence and integrated microarrays, quantitative detection of sST2 is 2 orders of magnitude lower than that of a traditional fluorescent immunoassay. The limit of detection is down to 10 pg/mL with the coefficient of variation being less than 8%. Detection of sST2 via fingertip blood is achieved in 10 min. Moreover, the P4 microarray after 180 days of storage at room temperature showed excellent stability for detection. This P4 microarray, as a convenient and reliable immunoassay for rapid and quantitative detection of protein markers in trace blood samples, has high sensitivity and strong storage stability, which hold great potential to advance cardiovascular precision medicine.

CAS / MDL: 99-20-7

研究领域: 生物医学工程 · 临床化学 · 心脏病学 · 即时检测

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Microfluidic point-of-care testing device for multiplexed detection of liver function blood markers

Fast and Sensitive Detection of Protein Markers Using an All-Printing Photonic Crystal Microarray via Fingertip Blood