ELECTROCHIMICA ACTA
·
2025
·
IF 5.6
Zhikun Zhang,Ying Shi,Zhengjie Li,Jilong Han,Zhiyan Chen,Fangfang Ma,Yanchao Qi,Yumin Liu
MicroRNA-21 (miR-21) has shown great promise as a biomarker for early cancer diagnosis. However, its trace-level abundance in clinical samples complicates point-of-care detection. Herein, we developed a new DNA-modified bimetallic dual-ligand MOF-based electrochemical sensor for highly sensitive and rapid detection of miR-21. The MOF was synthesized using 2,2′-bipyridine-5,5′-dicarboxylic acid (DPDC) and meso‑2,3-dimercaptosuccinic acid (DMSA) as organic linkers, with Ni²⁺/Co²⁺ serving as metal centers. Subsequently, the capture DNA (cDNA) was designed and modified MOF with high porosity and large surface area, significantly enhancing molecular interactions between the cDNA and target miR-21 to boost sensitivity. The developed biosensor exhibits a linear detection range of 10 fM to 100 nM, with a low detection limit of 10 fM and excellent specificity. This method effectively detects miR-21 in blood samples, showcasing strong clinical potential and providing a reliable platform for precision biosensing.
研究领域:
生物传感器
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纳米技术
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生物化学
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分子诊断学
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材料科学
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电化学
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肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL LETTERS
·
2022
·
IF 2.267
Caifen Chen,Dan Li,Baokang Jin
A selective, sensitive electrochemical biosensor is reported to determine bisphenol A (2,2-bis(4-hydroxyphenyl) propane, BPA) based on target-induced chain release. A gold electrode was premodified with dendritic gold nanoparticles (AuNPs) by direct electrochemical deposition to increase the active surface area of the electrode to immobilize hydrosulfonyl group-containing capture DNA (C-DNA). C-DNA was hybridized with a BPA-specific aptamer (T-DNA) to form a double helical structure. After the embedding of methylene blue (MB), this structure emitted measurable electrochemical signals. BPA specifically binds to T-DNA to form a stable BPA G-tetrad structure, thereby inducing the release of the aptamer chain and the embedded MB from the surface into solution, resulting in the reduction of the current signal. The constructed electrochemical biosensor provided a linear response to BPA concentration from 1.0 × 10−9 M to 5.0 × 10−7 M with a detection limit of 9.8 × 10−10 M. This strategy also provided good selectivity for BPA compared to similar interferents.
研究领域:
分析化学
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电化学
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生物传感器
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2019
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IF 4.916
Jingwen Gao,Ziyi Chen,Lebao Mao,Wei Zhang,Wei Wen,Xiuhua Zhang,Shengfu Wang
In this work, an innovative aptasensor based on electrochemiluminescence resonance energy transfer (ECL-RET) from CdTe quantum dots (QDs) to a cyanine dye (Cy5) fluorophore for the determination of Ochratoxin A (OTA) was fabricated. A strong cathodic ECL emission was obtained by the CdTe QDs modified glassy carbon electrode (GCE). After the immobilization with the capture DNA (cDNA) and the sequential hybridization with the probe DNA-modified Cy5 (pDNA, the aptamer of OTA), the ECL signal enhanced obviously through the ECL-RET. Meanwhile, the spectrum- and distance-related ECL enhancement effect was investigated. When the target OTA was in the presence, the pDNA-Cy5 molecules were released from the electrode surface owing to the specific interaction between OTA and aptamer, resulting in an evident decrease of ECL signal. Under optimal conditions, the developed aptasensor displayed the linear response toward OTA in the wide range of 0.0005–50 ng/mL with a low detection limit of 0.17 pg/mL. With the excellent selectivity , stability and repeatability, the strategy provided an efficient and universal method for the sensitive detection of target in practical application.
研究领域:
分析化学
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生物传感器
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毒理学
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食品安全
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 25
·2541-2548
·
IF 3.532
Lixia Su,Yonghuan Su,Bingqian Liu
A new ratiometric electrochemical strategy for immobilization-free detection of Escherichia coli (E. coli) was constructed by using a capture DNA-polyaniline/copper ferrite nanoparticles/graphene oxide (cDNA-PANI/CuFe2O4/GO) composite as capture probes, which has a high specific surface area and good magnetic properties. Then trigger DNA/Au nanoparticles (tDNA/Au NPs) were used as signal amplification labels, and Pt (IV) and Fe (III) were chosen as the signal probes. In the presence of targets, the sandwich format among cDNA-PANI/CuFe2O4/GO, E. coli and auxiliary DNA (aDNA) was realized by using the aptamer recognition system. Then, the tDNA/Au binding could be anchored on the sandwich format due to the principle of base complementation between unpaired aDNA and tDNA. And the unbounded tDNA of tDNA/Au NPs could bind an amount of Pt (IV). After separation using a magnet, a handful of unbound Pt (IV) which remained in the supernatant reacted with a large number of Fe (III) ions, leading to a markedly increased IFe(III)/IPt(IV) value. Oppositely, the sandwich format could not appear in the absence of targets, and even the tDNA/Au could not be immobilized on it. So, the redox reaction between a large amount of Pt (IV) residue in the supernatant and Fe (III) was significantly successful, causing a low IFe(III)/IPt(IV) value. Under optimal conditions, we found that IFe(III)/IPt(IV) was linearly related to the logarithmic E. coli concentration with a low limit of detection (1.862 × 103 cfu mL−1). This devised ratiometric electrochemical method may develop into a powerful and effective means for the detection of E. coli in real samples, which may also be developed as a universal tool for another microorganism.
研究领域:
分析化学
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电化学
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微生物学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）