JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Kai-Ni Wei,Gui-Xian Song,Shu-Zhen Huang,Qing Tang,Jian-Hang Hu,Zhu Tao,Ying Huang
In order to prevent and control the effects of pesticide residues on human health and the ecological environment, the rapid, highly sensitive, and selective detection of multiple pesticide residues has become an urgent problem to be solved. Herein, a lab-on-a-molecule probe based on a host–guest complex (ThT@Q[8] probe) has been developed to simultaneously analyze multiple aromatic pesticides under single wavelength excitation, such as fuberidazole, thiabendazole, carbendazim, thidiazuron, and tricyclazole. The fluorescence titration spectra of the ThT@Q[8] probe with the five pesticides mentioned above showed that the fluorescence intensity exhibited a good linear correlation with the pesticide concentration and the limit of detection was as low as 10–7 M. Because the ThT@Q[8] probe exhibits diverse fluorescence color changes to the five pesticides studied under a 365 nm ultraviolet lamp, we fabricated a single probe used to detect multiple analytes in the RGB triple channel by extracting the RGB variations. Principal component analysis and linear discriminant analysis proved that the ThT@Q[8] probe can recognize and distinguish five pesticides and can be applied at different concentrations. In real samples, the ThT@Q[8] probe recognized and distinguished five pesticides in tap water and Huaxi River water. The 1H NMR spectra results proved that a charge-transfer complex of ThT and pesticides in the Q[8] cavity may be formed. Moreover, we selected a test strip as a carrier to detect pesticides. The results indicate it can be used to quickly and conveniently detect different pesticides due to the rapid color change. Besides, the ThT@Q[8] probe has good cell permeability and can be used to detect pesticide residues in living cells. This work has laid the foundation for the qualitative and quantitative multitarget detection of pesticide residues.
研究领域:
分析化学
·
环境科学
·
分子生物学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 14
·1462-1470
·
IF 3.532
Jian Hua Wang,Yao Ming Liu,Jian Bin Chao,Yu Wang,Hui Wang,Shao Min Shuang
A new phenazine-imidazole based Schiff base (PIS) fluorescent probe has been developed for the ratiometric detection of Cd2+ ions in aqueous media at physiological pH. PIS upon binding with Cd2+ ions shows red shifted fluorescence and thereby, permits ratiometric detection of Cd(II) ions. A detection limit down to 2.10 × 10−8 M was determined for Cd2+ quantitation. Also, the accompanying apparent fluorescence color change (from yellow to orange red) is noticeable to the naked eye under a UV-lamp. The sensing mechanism could be attributed to the 1 : 1 PIS–Cd complexation, followed by extension of the conjugation due to better planarity and modulation of the charge transfer efficiency in the probe. This was complemented by solvatochromism and density functional theory calculations. Furthermore, PIS was used to detect Cd2+ in Oxya chinensis cells, zebrafish larvae and live tissues of Arabidopsis thaliana under a fluorescence microscope, showing great potential in analyzing living biosystems.
研究领域:
生物化学
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环境科学
·
分子生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2020
·
IF 3.306
Muhammad Amir Masood,Yuncong Chen,Shankun Yao,Shumeng Li,Weijiang He,Zijian Guo
A new BODIPY based Pd (II) complex, Pd-BDPA has been developed as a dual fluorometric and colorimetric probe for the detection of sulfide anion. The sensing ability is based on the interaction affinity of the Pd (II) towards the sulfide anion which is highly selective and immediate with a detection limit of 240 nM. The interaction between Pd (II) and sulfide is not only highly selective over the other common anions but also not interfered by their coexisting. This selective interaction between them leads to the regeneration of free ligand resulting in the dramatic changes in both fluorescence and absorption spectra. The fluorescent changes lead to quenching the emission spectra which is linear with the concentration of sulfide in the range of 1-10 μM. In addition to fluorescence quenching, the interaction also resulted in the significant color switching from purple to blue which was easily distinguished by the naked eye. Another important advantage is that the Pd-BDPA coated paper-based strips show the sulfide selectivity and sensitivity by the color changes as is in the solution form, revealing its practical utility more conveniently. In addition, the Pd-BDPA probe can also successfully be utilized for the determination of sulfide in real water samples with satisfactory recovery results indicating its feasibility for environmental samples.
研究领域:
分析化学
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环境科学
·
生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2024
·
IF 4.1
Shuo Xing,Jinli Ma,Fudong Zeng,Liangqiang Wu,Yixuan Yao,Yapeng Li,Jianshi Du,Qingbiao Yang,Yaoxian Li
Zinc is one of the essential trace elements in the human body and plays an extremely important role in physiological processes. Therefore, it is necessary to develop a detection method that can be used for real-time, highly sensitive response of Zn 2+ in organisms and has low toxicity. In this work, a novel fluorescent probe NA for Zn 2+ detection based on 1,8-naphthalene dicarboxylic anhydride and dimethylpyridine amine was synthesized. Probe NA has specific recognition and real-time monitoring functions for Zn 2+ . Based on Job's plotting curve, it is inferred that the coordination ratio between probe NA and Zn 2+ is 3:1, forming a complex of [Zn(NA) 3 ] 2+ . The [Zn(NA) 3 ] 2+ exhibits obvious yellow fluorescence at 550 nm. Probe NA can achieve quantitative detection of Zn 2+ with a detection limit of 7.52 × 10 −9 M, with high selectivity and anti-interference ability. Probe NA can stably exist in a wide pH range (2–10). This indicates that it can detect Zn 2+ in complex environments. In addition, based on the low cytotoxicity, high sensitivity, and real-time monitoring characteristics of probe NA, we have expanded it to real-time monitoring of Zn 2+ in organisms (HUVEC cells and live mice), as well as qualitative and quantitative detection of Zn 2+ concentration in food and drugs.
研究领域:
化学
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生物学
·
医学
✓ 已验证
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 9
·1545
·
IF 5.1
Caiwen Dong,Yaqin Li,Xincheng Sun,Xuehao Yang,Tao Wei
Vomitoxin is a member of the monotrichous mycotoxin family with a complex chemical structure and significant biological activity. This toxin has strong immunosuppressive toxic effects and can cause serious damage to human and animal health. In this study, an on-site immune detection method based on an immune SiO2@QD fluorescent probe was developed, which realized the rapid and quantitative detection of emetic toxins in grains. Polyethyleneimine (PEI) is a polymer containing a large number of amino groups, and the binding of PEI to the surface of quantum dots can serve to regulate growth and provide functionalized groups. A SiO2@QD nanotag with good dispersibility and a high fluorescence intensity was synthesized by combining a PEI interlayer on the surface of SiO2nanospheres. Utilizing the electrostatic adsorption of the amino group in PEI, CdSe/ZnS QDs were self-assembled on the surface of SiO2nanospheres. In the stability test, the SiO2@QDs could maintain basically the same fluorescence intensity for 90 consecutive days in the dark at 4 °C, showing a high fluorescence stability. The fluorescence-enhanced QD immune probe was formed by coupling with anti-DON monoclonal antibodies through carbodiimide chemical synthesis. For the detection of spiked wheat flour samples, the immuno-SiO2@QD fluorescent probe showed excellent sensitivity and stability, the detection limit reached 0.25 ng/mL, and the average recovery rate was 92.2–101.6%. At the same time, the immuno-SiO2@QD fluorescent probe is simple to operate, is capable of rapid responses, and has great potential in the rapid detection of vomitoxins in grains.
研究领域:
食品安全
·
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2020
·
IF 3.306
Fanyong Yan,Xiaodong Sun,Yan Zhang,Yingxia Jiang,Li Chen,Tengchuang Ma,Liang Chen
The form of a single response to a single target analyte limits the development of fluorescent probes . Here, a probe (HBTA) based on HBT (2-(2-Hydroxyphenyl)-benzothiazole) was synthesized to achieve a concept about nested probe, completing continuous detections. HBTA through low concentration Cu 2+ gives birth to a new probe (HBT-CHO) that can detect Cu 2+ by complexation and Cys by cyclization (HBT-Cys). These processes are accompanied by different fluorescent signal changes and can quantitatively detect Cys and Cu 2+ . Furthermore, HBT-CHO, HBT-Cys, and Cys can bind Cu 2+ , so competitive fluorescent response of Cu 2+ can take place through alternate addition of Cu 2+ and Cys. At this stage, the response becomes too complex to accurately quantify, but it is still dependable to predict changes in the content of the mixture system through competitive fluorescent changes. In summary, it is because of the existence of the nested pattern that HBTA completes multiple forms of responses to Cu 2+ and Cys.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF LUMINESCENCE
·
2018
·
IF 2.732
YaoYao Qu,Guojuan Ren,Liying Yu,Baoya Zhu,Fang Chai,Lihua Chen
Herein, the fluorescent carbon dots (CDs) has been synthesized by hydrothermal method by using ethylene diamine tetraacetic acid (EDTA) as a raw materials. The prepared CDs exhibited bright blue fluorscence excited by 340 nm with the quantum yield as high as 52%. The CDs possess special fluorescence responses to 2-nitrophenol (2-NP) and 4-nitrophenol (4-NP), and the slump of fluorescence was demonstrated by CDs solution in the case of 2-nitrophenol and 4-nitrophenol existence. The detection limit of 2-NP and 4-NP was calculated at 77 nM and 60 nM, respectively. Meanwhile, the color response to 2-NP and 4-NP can be observed from achromatic solution to yellow by naked eye. All in all, the obtained CDs can be applied as fluorescence and colorimetric probe in environmental protection.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Qi Dai,Zichun Qi,Zhenzhen Yan,Biao Yu,Jingqiu Li,Baosheng Ge,Hua He,Fang Huang,Xiaojuan Wang
A novel ratiometric fluorescent tyrosinase assay is developed based on hybrid nano-assembly of gold nanocluster and tyrosine-containing peptides. The AuNCs@YCY nano-probe ( AYNP ) is fabricated through the hydrophobic interactions and π-π stacking between the tyrosine residues of the Tyr-Cys-Tyr tripeptide (YCY) and the ligands on the surfaces of AuNCs under the near-isoelectric pH value. The resulted AYNP shows distinct fluorescence responses, spontaneous turn-on of the blue emission and turn-off of the near-infrared emission, with a single wavelength excitation. It is demonstrated that the enhancement and quenching are due to the production of pheomelanin and dopaquinone structures, respectively, induced by tyrosinase oxidation. The internal referencing system provides the tyrosinase assay with superior sensitivity and a detection limit as low as 6.3 U L −1 could be achieved. The experimental results also demonstrate the excellent selectivity, good photo-stability, and both in vitro and cellular applications of AYNP . This assay technique is low-cost, easy to prepare, and shows excellent potential as a novel melanoma clinical diagnostic platform and a tyrosinase inhibitor screening tool.
研究领域:
生物化学
·
医学诊断
·
药物筛选
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Tiantian Kang,Haoping Wang,Xiaoju Wang,Liheng Feng
A simple fluorescent probe comprised of coumarin and quinoline units was designed and synthesized through acylhydrazine bonding mode. On account of the “push-pull” structure, the probe exhibits high selectivity and sensitivity for Zn(II) with an obvious red-shift of fluorescence and a visible color change. The detection of Zn(II) by the probe is almost free from interference of other ions and reversible based on intramolecular charge transfer process. Three five-membered rings of the probe with Zn(II) were formed and verified by MS and 1 H NMR spectra. Notably, as a simple, fast and environmentally friendly device, detection of ions by test paper has great practicality, the probe could be designed as test papers for detecting Zn(II) taking advantage of the color change character under 365 nm UV lamps.
研究领域:
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Caihong Zhou,Yongzhao Zhang,Xuejing Yang,Ziqian Zhao,Menglei Xia,Chunhui Wei,Qiankun Wu,Zisong Chang,Long Ma,Lijuan Yin
Background Norovirus (NoV) is the leading cause of foodborne disease outbreaks worldwide, typically spreading via contaminated food and water. Rapid, sensitive, and portable detection of NoV is crucial. Results Here, we presented a magnetic CRISPR/Cas12a-SERS nanobiosensor capable of detecting NoV with high sensitivity, accuracy, speed, and portability. In this nanobiosensor, SERS nanoprobes linked to magnetic nanoprobes via linker single-stranded DNAs (ssDNAs). The presence of NoV nucleic acid triggered Cas12a's trans -cleavage activity, degrading the linker ssDNA. After magnetic separation, the dissociated SERS nanoprobes were efficiently separated from the magnetic nanoprobes. This enhanced the SERS signal in the supernatant, detectable using a portable Raman spectrometer. The detection limit for NoV is 100 copies/mL within 60 min. The nanobiosensor was further assessed in real-world settings, demonstrating excellent sensitivity and selectivity for detecting trace NoV in complex food samples. Significance This approach not only broadens CRISPR-based pathogen detection but also provides a reliable tool for monitoring foodborne viruses. Its potential extends beyond NoV, promising enhanced surveillance of various pathogens in food safety, environmental monitoring, and public health sectors.
研究领域:
生物技术
·
食品安全
·
分子诊断
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 22
·9604-9608
·
IF 1.821
Yanhua Yu,Changzhi Dong
In this paper, an efficient colorimetric and fluorescent probe based on a benzothiadiazole derivative was developed for the detection of fluoride ions (F−). The selectivity of this approach capitalized on the highly selective and straightforward chemical reaction between F− and the Si–O bond. The sensing behavior of this probe towards anions was monitored by UV-visible and fluorescence spectroscopies in acetonitrile–aqueous buffer mixtures. Among the tested anions: F−, Cl−, Br−, I−, ClO4−, NO3−, CN−, HSO4−, AcO− and H2PO4− only F− induced a remarkable red-shift (149 nm) in the absorption spectrum which is concomitant with a color change from colorless to pink. This probe provides a new approach for naked-eyed detection of F− and the detection limit was calculated to be 1.7 μM. Besides, a concomitant fluorescence decrease was also observed. These results demonstrated that the probe could detect F− with excellent sensitivity and selectivity in complicated samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 40
·24822-24827
·
IF 3.108
Ting Yu,Guoxing Yin,Peng Yin,Ying Zeng,Haitao Li,Youyu Zhang,Shouzhuo Yao
We have synthesized a novel long-wavelength fluorescent probe MFC for detection of palladium (Pd0). This probe not only shows high selectivity for palladium, but also could quickly discriminate different palladium species (Pd0, Pd2+, Pd4+) in phosphate buffered solution. After the reaction with Pd(PPh3)4, the UV absorption of the probe MFC shifts from 334 nm to 594 nm within a few minutes. The probe MFC conjugated with alkyl carbamate, which destroys the principle of intramolecular charge transfer (ICT) and shows weak fluorescence. Based on the Pd0-catalyzed Tsuji–Trost deallylation reaction, the fluorogen was released and the fluorescence intensity at 612 nm was observed with 13-fold enhancement along with an obvious color change from yellow to purple with low detection limit.
研究领域:
分析化学
·
材料科学
·
催化化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）