IEEE SENSORS JOURNAL
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2024
·Vol. 24
·期 24
·40904-40911
·
IF 4.3
Xuegang Li,Pengqi Gong,Xue Zhou
Deoxyribonucleic acid (DNA) detection represents an important direction of diagnosis and treatment of genetic diseases, which contains all the genetic information of the human body. The DNA sequence remains extremely challenging to detect, despite numerous previous studies that have achieved selective detection, due to the backward sensing scheme. For low-cost, high-sensitivity DNA hybridization detection, a probe-type highly sensitive DNA detection method based on surface plasmon resonance (SPR) is demonstrated in this article. First, low-cost refractive index (RI) tracking detection can be completed via optical fiber SPR. Then, the selective detection of complementary DNA (cDNA) is achieved by binding the probe DNA (pDNA) sequence to the optical fiber SPR sensor using polyethyleneimine (PEI). Compared with previous studies, the sensitivity [0.13 nm/(nmol/L)] is greatly upgraded, and the detection limit (DL) is reach to 4.87 nmol/L. This low-cost and high-performance optical fiber sensing method is conducive to the further development of DNA hybridization detection technology.
研究领域:
生物医学工程
·
分子生物学
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DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Cai-Xia Shi,Yan-Zi Li,Qing Liu,Zeng-Ping Chen,Sha-Sha Li,Ru-Qin Yu
Biosensors based on various spectroscopic techniques discriminate the target microRNA (miRNA) from non-target ones with single nucleotide polymorphisms (SNPs) according to the differences in signal intensities which can be caused by other factors besides SNPs. As a result, they are liable to produce false positive results. Herein, we report an attempt to develop a false-positive resistance, sensitive and reliable mass spectrometric platform for miRNA detection. In the proposed platform, the qualitative and quantitative information of the target miRNA was obtained through analyzing mass spectral responses of the multiply charged ions of the residual fragments of the probe DNA produced during exonuclease III assisted signal amplification reaction using an advanced data analysis method. The proposed platform could achieve sensitive and accurate quantitative results for the target miRNA (e.g., miRNA-141) in complex medium with a detection limit of about 1 pM, and unambiguously identify non-target miRNAs with SNPs based on the length distribution patterns of residual fragments of probe DNA. The findings obtained in this study might open an avenue for the design of new miRNA detection methods based on mass spectrometry in combination with various nuclease assisted signal amplification strategies.
研究领域:
生物化学
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分子生物学
·
分析化学
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DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2015
·Vol. 162
·期 12
·B291
·
IF 3.266
Feng Gao,Xili Cai,Hidekazu Tanaka,Qionghua Zhu,Fei Gao,Qingxiang Wang
An ultrasensitive DNA biosensor was constructed in this work by using graphene oxide nanosheets (GONs) as the multi-site platform for probe DNA immobilization. First, L-cysteine (L-cys) was self-assembled on a gold electrode surface based on the Au-S chemistry. Then the GONs and amino modified probe DNA was covalently grafted to L-cys/AuE surface in turn, with the aid of 1-(3-Dimethylanminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-Hydroxy succinimide (NHS). The layer upon layer assembly process was characterized with atomic force microscopy and electrochemical methods. Due to the nano-size effect and multiple site characteristic of GONs, the immobilization density of the probe DNA was determined to be as high as 5.72 × 10−10 mol cm−2 using methylene blue as the redox probe. The hybridization experiments showed that the biosensor can quantitatively detect target DNA in a wide range from 1.0 × 10−15 M to 1.0 × 10−9 M with a detection limit of 5.0 × 10−16 M. The selectivity experiments showed that the sensing system could accurately discriminate the complementary sequence from the base mismatched and non-complementary sequences.
研究领域:
生物技术
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纳米生物学
·
分析化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Songling Li,Weiwei Qiu,Xuan Zhang,Jiancong Ni,Feng Gao,Qingxiang Wang
An ultrasensitive electrochemical DNA biosensor has been developed based on the assembly of highly conductive gold nanoparticles (AuNPs) on the free terminal of hairpin-structured probe DNA . The hairpin probe DNA was first immobilized on a gold electrode surface through Au S bond. Then the thioglycolic acid (TGA) was grafted on the 3′-end of hairpin DNA via the carboxylic-amino condensation reaction . Followed by, the AuNPs were further attached through reaction with the thiol-group of TGA. Thus, a highly conductive biointerface with ultralow charge-transfer resistance was obtained. Quantitative analysis exhibited that the target DNA could be detected over the range from 1.0 × 10 −17 to 1.0 × 10 −11 M. The detection limit was estimated to be 1.7 × 10 −18 M. Also the biosensor showed good hybridization selectivity , stability and regeneration ability.
研究领域:
生物化学
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纳米生物学
·
分析化学
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产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2015
·
IF 2.587
Xiaoqian Chen,Qingxiang Wang,Liheng Wang,Hongxu Guo,Yizhen Yang,Feiming Li,Feng Gao
Rugby-ball-shaped CoS2 (rCoS2) submicroparticles have been synthesized via a simple hydrothermal reaction. The rCoS2 submicroparticles were then casted on a glassy carbon electrode and served as a highly conductive and large surface area platform for the electrosynthesis of poly(2-thiophenesulfonyl chloride) (pTSC), using 2-thiophenesulfonyl chloride as the monomer. The stepwise modification process was characterized by attenuated total reflectance–Fourier transform infrared spectroscopy (ATR-FTIR) and different electrochemical technologies. The stable pTSC layer was utilized as a functional interface to immobilize amino-modified probe DNA via a facile sulfonyl–amino condensation reaction, without the assistance of any other cross-linkers. Because of the synergistic effect of rCoS2 and pTSC from the hybrid material, the developed DNA biosensor could detect target DNA over a wide dynamic range, from 1.0 × 10–13 to 1.0 × 10–7 M, with the detection limit down to 1.1 × 10–14 M. Benefiting from the steady electropolymerization film of pTSC and the stable sulfamide bond between the amino-modified DNA and pTSC, the biosensor also showed high stability, reusability, and reproducibility.
研究领域:
生物化学
·
材料科学
·
分析化学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Longshua Qin,Ting Huang,Hanfeng Cui,Mengqing Cheng,Guobing Wei,Fusheng Liao,Wei Xiong,Hedong Jiang,Jing Zhang,Hao Fan
Herein, an fluorescence (FL)−electrochemiluminescence (ECL) dual-mode biosensor is constructed based on the dual-signal “turn-on” strategy of functionalized metal-organic frameworks nanosheets (RuMOFNSs)-tetraferrocene for K-ras gene detection, and the mechanism of bursting through front-line orbital theory is explained for the first time. Amino-functionalized tetraferrocene-labeled probe DNA molecules are linked to RuMOFNSs by covalent amide bonds, acting as FL and ECL intensity switches. The target DNA, complementary to the probe DNA, triggers cyclic amplification of the target by nucleic acid exonuclease III (Exo III), repelling tetraferrocene reporter groups away from RuMOFNSs and inhibiting the electron transfer process and photoinduced electron transfer (PET) effect. These phenomena induce a double turn-on of FL and ECL signals with a high signal-to-noise ratio. The developed FL-ECL dual-mode sensing platform provides sensitive detection of the K-ras gene with detection limits of 0.01 fM (the detection range is 1 fM to 1 nM) and 0.003 fM (the detection range is 0.01 fM to 10 pM), respectively. In addition, the proposed dual-mode sensor can be easily extended to detect other disease-related biomarkers by changing the specific target and probe base sequences , depicting potential applications in bioanalysis and early disease diagnosis.
研究领域:
生物化学
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分析化学
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生物技术
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
INFRARED PHYSICS & TECHNOLOGY
·
2025
·
IF 3.4
Lixia Li,Yurui Huang,Jiabin Zhao,Feiyou Liu,Ning Feng,Yufang Liu
Mercury ions (Hg 2+ ) are highly toxic heavy metal pollutants that pose significant risks to the environment and human health. In this paper, a fiber optic surface plasmon resonance (SPR) sensor is proposed for ultra-sensitive detection of Hg 2+ . The probe features Ag/ Ge 2 Sb 2 Te 5 (GST)/Ag composite film and metal–organic framework (MOF)-sensitized layer functionalized with probe DNA (pDNA). In the presence of Hg 2+ , pDNA interacts with target DNA (tDNA) carrying gold nanoparticles (AuNPs) in the solution through the T-Hg 2+ -T structure, immobilizing AuNPs on the sensing surface and inducing a spectral red shift. By combining GST’s high dielectric constant and MOF’s large surface area with strong pDNA affinity, the sensor achieves a maximum refractive index (RI) sensitivity of 11471 nm/RIU, detecting Hg 2+ as low as 10 pM. Furthermore, the deep learning model based on the residual neural network (ResNet) is employed to classify and recognize the SPR spectra corresponding to six distinct concentrations of Hg 2+ , achieving a training accuracy of 99.88 % and a test accuracy of 97.5 %. Therefore, ultra-sensitive fiber optic SPR sensor with deep learning delivers high-performance sensing while enabling rapid, precise, and sensitive quantification of Hg 2+ detection.
研究领域:
传感器
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环境监测
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光学传感
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纳米技术
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重金属检测
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机器学习
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产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Jingwen Gao,Ziyi Chen,Lebao Mao,Wei Zhang,Wei Wen,Xiuhua Zhang,Shengfu Wang
In this work, an innovative aptasensor based on electrochemiluminescence resonance energy transfer (ECL-RET) from CdTe quantum dots (QDs) to a cyanine dye (Cy5) fluorophore for the determination of Ochratoxin A (OTA) was fabricated. A strong cathodic ECL emission was obtained by the CdTe QDs modified glassy carbon electrode (GCE). After the immobilization with the capture DNA (cDNA) and the sequential hybridization with the probe DNA-modified Cy5 (pDNA, the aptamer of OTA), the ECL signal enhanced obviously through the ECL-RET. Meanwhile, the spectrum- and distance-related ECL enhancement effect was investigated. When the target OTA was in the presence, the pDNA-Cy5 molecules were released from the electrode surface owing to the specific interaction between OTA and aptamer, resulting in an evident decrease of ECL signal. Under optimal conditions, the developed aptasensor displayed the linear response toward OTA in the wide range of 0.0005–50 ng/mL with a low detection limit of 0.17 pg/mL. With the excellent selectivity , stability and repeatability, the strategy provided an efficient and universal method for the sensitive detection of target in practical application.
研究领域:
分析化学
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生物传感器
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毒理学
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食品安全
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 36
·14327-14335
·
IF 3.069
Wancun Zhang,Fang Hu,Xianwei Zhang,Wei Meng,Yaodong Zhang,Yinsen Song,Huanmin Wang,Peng Wang,Yueqing Gu
In this study, a simple, robust, label-free electrochemical biosensor based on the ligase chain reaction (LCR) was developed for the highly sensitive and selective detection of single nucleotide polymorphisms (SNPs) using Fe(CN)63−/Fe(CN)64− as a redox indicator. High sensitivity and specificity were achieved by using the LCR, which employs a thermostable and single-base discerning Hifi Taq DNA ligase to repeatedly ligate adjacently hybridized LCR-specific probes during thermal cycles, resulting in the highly specific exponential amplification of DNA targets. The electrochemical signal of the biosensor was further decreased using stem-loop probe DNA (SL-DNA), which specifically hybridized with LCR products on the electrodes. The linear dynamic range and high sensitivity of the mutant DNA (MutDNA) and wild-type DNA (WtDNA) LCR-based electrochemical biosensors for the detection of MutDNA and WtDNA were studied in vitro, with a broad linear dynamic range of 10 aM to 10 pM and detection limits of 1.18 aM and 0.35 aM, respectively. In addition, the MutDNA and WtDNA LCR-based electrochemical biosensors were able to accurately detect allele frequency changes as low as 0.1%. In summary, the developed LCR-based electrochemical biosensor provides a useful platform to monitor SNPs in a highly sensitive and specific manner.
研究领域:
生物传感
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分子生物学
·
电化学
·
遗传分析
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Wang Ren,Zhong Feng Gao,Nian Bing Li,Hong Qun Luo
This work reported a novel, ultrasensitive, and selective platform for electrochemical detection of DNA , employing an integration of exonuclease III (Exo-III) assisted target recycling and hybridization chain reaction (HCR) for the dual signal amplification strategy. The hairpin capture probe DNA (C-DNA) with an Exo-III 3′ overhang end was self-assembled on a gold electrode. In the presence of target DNA (T-DNA), C-DNA hybridized with the T‐DNA to form a duplex region, exposing its 5′ complementary sequence (initiator). Exo-III was applied to selectively digest duplex region from its 3-hydroxyl termini until the duplex was fully consumed, leaving the remnant initiator. The intact T-DNA spontaneously dissociated from the structure and then initiated the next hybridization process as a result of catalysis of the Exo-III. HCR event was triggered by the initiator and two hairpin helper signal probes labeled with methylene blue , facilitating the polymerization of oligonucleotides into a long nicked dsDNA molecule. The numerous exposed remnant initiators can trigger more HCR events. Because of integration of dual signal amplification and the specific HCR process reaction, the resultant sensor showed a high sensitivity for the detection of the target DNA in a linear range from 1.0 fM to 1.0 nM, and a detection limit as low as 0.2 fM. The proposed dual signal amplification strategy provides a powerful tool for detecting different sequences of target DNA by changing the sequence of capture probe and signal probes, holding a great potential for early diagnosis in gene-related diseases.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
分子生物学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Biyan Xu,Delun Zheng,Weiwei Qiu,Feng Gao,Shaoxiong Jiang,Qingxiang Wang
In this work, a novel electrochemical DNA biosensor has been developed based on the hybrid film of fern leaf-like α-Fe 2 O 3 microparticles and chitosan (CS). The fern leaf-like α-Fe 2 O 3 microparticles were synthesized via a facile template-free hydrothermal method, and their morphologies were characterized by X-ray diffraction, energy dispersive spectrometry, scanning electron microscope, and transmission electron microscope. Electrochemical characterization assays revealed that the hybrid film modified electrode had remarkable synergistic effects of the large accessible surface area and high electrical conductivity of semiconductive Fe 2 O 3 , and the good film stability of CS. Based on the rich amino groups on CS, the CS–Fe 2 O 3 hybrid film was employed as a functional matrix for probe DNA immobilization using terephthalaldehyde (TPA) as a bifunctional arm-linker. The hybridization capacity of the developed biosensor was evaluated with electrochemical impedance spectroscopy (EIS) using [Fe(CN) 6 ] 3−/4− as the indicating probe. A wide dynamic detection range from 1.0×10 −14 to 1.0×10 −10 M with ultralow detection limit of 5.6×10 −15 M was achieved for the target DNA. The hybridization selectivity experiments further revealed that the biosensor could discriminate fully complementary sequences from one-base mismatched, three-base mismatched, and non-complementary sequences. Moreover, the biosensor showed the advantage of good regeneration ability and reproducibility.
研究领域:
生物化学
·
电化学
·
纳米技术
·
生物传感器
·
材料科学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 182
·期 1
·241-248
·
IF 3.719
Wang Mo,Yang Zhiqing,Guo Yunlong,Wang Xinxu,Yin Huanshun,Ai Shiyun
We demonstrate a photo-electrochemical biosensor for the sensitive and specific detection of microRNA using Bi 2 S 3 nanorods as a photoactive material and streptavidin as the unit that inhibits photocurrent. Bi 2 S 3 nanorods were synthesized hydrothermally in organic phase and displayed excellent light-to-current conversion efficiency. The Bi 2 S 3 was deposited on an indium tin oxide (ITO) slice and then modified with gold nanoparticles onto which biotinylated hairpin probe DNA was deposited as a monolayer. Following hybridization between the biotinylated probe DNA and the target microRNA, the stem-loop structure of the probe DNA was unfolded and the biotin directed outwards into the solution. Streptavidin was then added to bind to biotin via the strong streptavidin-biotin interactions. This causes the photocurrent of the modified ITO to decrease due to steric hindrance that blocks the transfer of electrons from added ascorbic acid to the surface of the electrode. The method has a detection limit as low as 3.5 fM of microRNA and can excellently discriminate even singly mismatched microRNA. The method was successfully applied to investigate the effect of abscisic acid on the expression level of microRNA-159a in seeds of Arabidopsis thaliana . We conclude that the assay presented here has a large potential as a method for quantification of microRNA and for studying the epigenetic regulation of flowering plants.
The photocurrent generation and decrease mechanism of the PEC biosensor.
研究领域:
生物传感器
·
光化学
·
纳米技术
·
分子生物学
·
植物科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）