PACKAGING TECHNOLOGY AND SCIENCE
·
2023
·Vol. 36
·期 11
·941-950
·
IF 2.6
Wenjiao Ge,Han Yu,Xiaohui Wang
Natural antioxidants are beneficial additives for active packaging due to their ability to resist oxidative damage. Polymeric micelles are effective in delivering natural antioxidants in aqueous environments. However, individual encapsulation of a single antioxidant may not be sufficient to meet the needs of modern food packaging. A dual natural antioxidant-loaded micellar system (i.e., vitamin E [VE] and curcumin [Cur]) was developed to achieve combined functionality and high loading concentration. Chitosan-graft-oleic acid (CSOA) micelles were employed as carriers to co-encapsulate VE and Cur through a facile ultrasound-assisted self-assembly method. The prepared VE and Cur co-loaded micelles (CSOA/VE/Cur) exhibited a uniform spherical structure with an average particle size of 210.6 nm and a zeta potential of +29.5 mV. Moreover, the co-encapsulation of VE and Cur showed a significant enhancement in both entrapment efficiency, loading capacity and stability as compared to the individual encapsulation of either VE or Cur. In addition, the CSOA/VE/Cur micelles showed improved antioxidant activity than free VE/Cur at the same concentrations, as evidenced by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Moreover, the micellar materials were utilized to fabricate regenerated cellulose (RC) films with antioxidant properties, highlighting their potential as additives for antioxidant active packaging.
研究领域:
食品科学
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材料科学
·
包装工程
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Ling Tang,Jinqing Chen,Jiali Luo,Huijuan Wei,Lina Lan,Hailan Qin,Qiumei He,Xiangyu Kong,Liting Lai,Wenya Ding,Jian Xiao
Dihydromyricetin (DMY), the main functional component of vine tea, exhibits multiple biological activities and is widely used in food applications such as dairy products, functional foods, and food additives. However, its poor stability and bioavailability limit its broader utilization. To address this, CCGT-DMY-NPs were successfully prepared using chitosan-grafted-β-cyclodextrin (CS-CD), gelatin-grafted-β-cyclodextrin (Gel-CD), and vitamin E polyethylene glycol succinate (TPGS). The CCGT-DMY-NPs exhibited a particle size of 216.33 ± 7.23 nm, a polydispersity index of 0.207 ± 0.011, a zeta potential of 25.67 ± 4.53 mV, and an encapsulation efficiency of 98.21 ± 2.44 %, with significantly improved stability compared to free DMY. In vitro cellular uptake studies using confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) confirmed enhanced uptake of CCGT-DMY-NPs in Caco-2 cells. In vitro unidirectional intestinal perfusion demonstrated that CCGT-DMY-NPs significantly increased the apparent permeability coefficient (P app ) and absorption rate constant (Ka) across duodenal, jejunal, and ileal segments ( p < 0.01), with jejunal P app reaching 2.47 ± 0.59 × 10 −2 cm/min compared to 0.26 ± 0.06 × 10 −2 cm/min for free DMY. Mechanism studies indicated that the enhanced absorption was attributed to mucoadhesion, tight junction opening, and involvement of multiple endocytic pathways (clathrin-, caveolin-, non-caveolin-, and non-clathrin-mediated) and macropinocytosis. Pharmacokinetic studies confirmed that the area under the curve (AUC 0-t ) of CCGT-DMY-NPs was 2.20 times higher than that of oral DMY, and the relative bioavailability (F rel ) of CCGT-DMY-NPs reached 219.89 %. These findings suggest that CCGT-DMY-NPs significantly enhance the oral bioavailability of DMY through multi-mechanistic promotion of intestinal absorption, providing a promising strategy for its application in functional foods.
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Hengjun Zhou,Chenyu Liu,Simiao Yu,Farishta Shafiq,Weihong Qiao
Despite paclitaxel (PTX) as the first-line anti-tumor drugs, the unsatisfactory low-solubility in water remained a barrier to clinical application. Thus, the present study aimed at enhancing the solubility of PTX. Accordingly, we designed the amphiphilic conjugates (HP) as the delivery system and prepared self-assembly nanoparticles by the conjugates. Hemolysis assays showed the good biocompatibility of the conjugates. Meanwhile, binding affinity between the conjugates and PTX was explored based on the critical aggregation concentration (cac). The biggest solubilization for the nanoparticles was 193.56-fold, which exhibited as the solubility of 48.39 μg/mL. Cellular uptake assays revealed the valid internalization of nanoparticles and the targetability. In addition, the nanoparticles arrested the mitosis and further apoptosis. Cytotoxicity and three-dimensional multicellular spheroid (3DMCS) assays proved the strong growth inhibition capacity and the superior penetration ability for the nanoparticles. These results suggested that the HP conjugate we synthesized could provide potential insights into the delivery system for hydrophobic drugs on the aspects of anti-tumor.
研究领域:
药学
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纳米技术
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癌症研究
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药物递送系统
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Liyun Rui,Minhao Xie,Bing Hu,Li Zhou,Muhammad Saeeduddin,Xiaoxiong Zeng
Chlorogenic acid-chitosan conjugate was synthesized by introducing of chlorogenic acid onto chitosan with the aid of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and hydroxybenzotriazole. The data of UV–vis, FT-IR and NMR for chlorogenic acid-chitosan conjugates demonstrated the successful conjugation of chlorogenic acid with chitosan. Compared to chitosan, chlorogenic acid-chitosan conjugates exhibited increased solubility in distilled water, 1% acetic acid solution (v/v) or 50% ethanol solution (v/v) containing 0.5% acetic acid. Moreover, chlorogenic acid-chitosan conjugates showed dramatic enhancements in metal ion chelating activity, total antioxidant capacity, scavenging activities on 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) and superoxide radicals, inhibitory effects on lipid peroxidation and β-carotene-linoleic acid bleaching, and protective effect on H 2 O 2 -induced oxidative injury of PC12 cells. Particularly, chlorogenic acid-chitosan conjugate exhibited higher inhibitory effects on lipid peroxidation and β-carotene-linoleic acid bleaching than chlorogenic acid. The results suggested that chlorogenic acid-chitosan conjugates could serve as food supplements to enhance the function of foods in future.
研究领域:
食品科学
·
化学
·
药学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）