DRUG DELIVERY
·
2021
·
IF 6.419
Lianghui Zhao,Xia Qi,Tao Cai,Zheng Fan,Hongwei Wang,Xianli Du
Corneal wound healing is a highly regulated biological process that is of importance for reducing the risk of blinding corneal infections and inflammations. Traditional eye drop was the main approach for promoting corneal wound healing. However, its low bioavailability required a high therapeutic concentration, which can lead to ocular or even systemic side effects. To develop a safe and effective method for treating corneal injury, we fabricated rutin-encapsulated gelatin hydrogel/contact lens composites by dual crosslinking reactions including in situ free radical polymerization and carboxymethyl cellulose/N-hydroxysulfosuccinimide crosslinking. In vitro drug release results evidenced that rutin in the composites could be sustainedly released for up to 14 days. In addition, biocompatibility assay indicated nontoxicity of the composites. Finally, the effect of rutin-encapsulated composites on the healing of the corneal injury in rabbits was investigated. The injury was basically cured in corneas using rutin-encapsulated composites (healing rate, 98.3% ± 0.7%) at 48 h post-operation, while the damage was still present in corneas using the composite (healing rate, 87.0% ± 4.5%). Further proteomics analysis revealed that corneal wound healing may be promoted by the ERK/MAPK and PI3K/AKT signal pathways. These results inform a potential intervention strategy to facilitate corneal wound healing in humans.
研究领域:
生物材料学
·
眼科医学
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2017
·
IF 1.9
Wanlu Zhang,Guangji Li,Yinlei Lin,Liying Wang,Shuqing Wu
We aimed to introduce hydrophilic sulfobetaine-type zwitterionic groups to macromolecular chains of copolymers to construct novel copolymer hydrogels with anti-protein-fouling performance that could be used as soft contact lens (SCL) materials. Using hydroxyethyl methacrylate (HEMA), N-vinyl pyrrolidinone (NVP) and sulfobetaine methacrylate (SBMA) as comonomers, several copolymer hydrogels with different SBMA contents, poly(HEMA-NVP-SBMA), are synthesized via radical copolymerization in an aqueous phase. Surface chemistry, structural morphologies, water contact angle (WCA), equilibrium water content (EWC), visible light transmittance and tensile mechanical properties are investigated. The prepared hydrogels exhibit a closed-type porous structure. With increasing SBMA content in the comonomer mixture, the prepared copolymer hydrogel pore size gradually increases up to the micron level, WCA tends to decrease, EWC tends to increase, and visible light transmittance slightly increases, but their tensile mechanical properties decline. The amounts of protein Lyz and BSA adsorbed on the copolymer hydrogels and on commercially available EASY DAY® SCLs as a control are also determined by protein adsorption tests. The amount of protein adsorbed on the copolymer hydrogel decreases with increasing SBMA content. For the hydrogel prepared using the comonomer mixture with 5.0 wt % SBMA, the amount of adsorbed Lyz is 0.91 μg/cm2, which corresponds to only 56.8% of the amount adsorbed on EASY DAY® SCLs. Thus, novel SCL materials with high water content and excellent anti-protein-fouling performance were efficiently constructed by introducing sulfobetaine-type zwitterionic groups into a traditional polymer hydrogel system.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
DRUG DELIVERY
·
2021
·
IF 6.419
Caiqing Wu,Ping Wai Or,Jones Iok Tong Chong,Isuru K. K. Pathirage Don,Ching Hymn Christopher Lee,Kaili Wu,Minbin Yu,David C. C. Lam,Yangfan Yang
To increase the amount of pirfenidone (PFD) loaded in polyvinyl alcohol (PVA) film embedded soft contact lens (SCL), and evaluate its function of sustaining delivery of drug in vitro and in vivo. Drug loading efficiency within PVA film and SCLs, drug release from SCLs in vitro, and the effects of parameters of SCLs and external environment on drug release in vitro were evaluated by ultraviolet–visible spectrophotometer at 312 nm. Safety of SCLs was evaluated in vitro by transformed human corneal epithelial cell. Safety in vivo was determined by optical coherence tomography and histology of anterior segment of rabbits. Drug release study in tear fluid and aqueous humor were measured by ultra-performance liquid chromatography. SCLs had smooth surface and were fit for experimental rabbits. Amount of PFD in PVA film and SCLs were 153.515 μg ± 12.508 and 127.438 μg ± 19.674, respectively, PFD in PVA film was significantly higher than SCLs (p=.006) and closed to 150 μg (targeting amount of PFD to be loaded). Thickness of SCLs, molecular weight of PVA, and amount of PVA used in SCLs affected drug release in vitro significantly. Thickness of PVA film and amount of drug in SCLs had no effect on drug release rate in vitro. SCLs were safe in vitro and in vivo, PFD released from SCLs could be detected around 12 hours in tears and aqueous humor, and the concentration of drug was higher than eye drop at all detected time points while amount of PFD in SCLs was lower than eye drop. Drug loaded PVA film embedded SCLs may be a promising ocular drug delivery system.
研究领域:
药学
·
眼科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Materials
·
2021
·
IF 3.515
Lin Xiaoxia,Zhang Haitao,Qin Yuqing,Hu Xiaohong
Traditional hydrogel has usually had restricted application in the biomedical field due to lack of a control unit. Herein, Azobenzene (AZO), as a typical kind of photo-response molecule, was introduced into hydrogel by polymerization to provide a control unit for hydrogel. Simultaneously, the immobilization of AZO end group would solve the problems of uncontrollable recovery and light fatigue for AZO small molecules according to previous research. In the research, two kinds of crosslinkable AZO molecules, namely AZO crosslinker and AZO monomer, were synthesized as functional monomers or crosslinkers. Further, AZO copolymerized hydrogel and AZO crosslinked hydrogel were obtained. In order to estimate fundamental properties for contact lenses, gelation time and swelling ratio of hydrogels as a function of AZO concentration as well as crosslinking type were investigated. Moreover, synthesized hydrogels exhibited typical porous morphology, but their size and homogeneity were dependent on the type of hydrogel. In order to evaluate photo-responsive performance, detailed photo induced isomerization of both AZO copolymerized hydrogel and crosslinked hydrogel were tracked by UV spectra. The results confirmed the reversible, stable, and controllable photo responsive process. In vitro evaluation was used to investigate drug release behavior using orfloxacin and puerarin as model drugs. It was found that AZO addition as well as hydrogel type would influence puerarin release, but would have little effect on the orfloxacin release behavior in hydrogels. Furthermore, the isomer type in the hydrogel would have some effects on drug release behavior including orfloxacin and puerarin.
研究领域:
生物医学工程
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材料科学
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药剂学
✓ 已验证
产品关联
DOI
高影响因子
Materials
·
2025
·Vol. 18
·期 4
·827
·
IF 3.2
Yamin Chen,Dianyang Li,Yougen Chen,Hui Fang
Four kinds of silicone hydrogel transparent contact lenses (CLs) with different formulations were prepared by the free radical photocuring polymerization. By mixing polyethylene glycol diacrylate (PEGDA) of 1000 Da with ethylene glycol dimethacrylate (EGDMA) and adding other silicone monomers and hydrophilic monomers, the transparency and flexibility of the material were successfully achieved. By optimizing the weight percentage of each component, the best balance of optical performance can be achieved. The photocuring properties of the materials were characterized by electronic universal test, double-beam UV-visible spectrophotometer, Atomic Force Microscope (AFM), Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). The results showed that the addition of higher PEGDA content reduces the elastic modulus, improves curing efficiency, improves equilibrium water content (EWC), and enhances light transmission. Hydrogels containing only high PEGDA but no EGDMA showed similar curing rates, water content, and elastic modulus, but had the worst optical transparency, far inferior to the materials mixed with PEGDA and EGDMA. Additionally, imaging performance of the CLs was further evaluated through simulation analysis using Ansys Zemax OpticStudio2024 software. This research provides a new choice of material consideration to improve the performance and wearing comfort of CLs.
研究领域:
材料科学
·
生物医学工程
·
眼科研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Heliyon
·
2023
·
IF 4
Malake Sarmout,Yutang Xiao,Xiao Hu,Aiym Rakhmetova,Leo H. Koole
Soft contact lenses are increasingly being explored as a vehicle for controlled delivery of ophthalmic drugs. However, traditional methods of drug-loading by soaking have limitations such as burst delivery and the release of drugs at the front side of the lens, leading to poor drug efficacy and systemic side effects. This study introduces a new methodology, termed asymmetric drug loading, whereby the ophthalmic drug ‘Rebamipide’ is attached to and released from the post-lens (=cornea-contacting) surface exclusively. The methodology involves using polymeric microparticles that carry a lipophilic crystalline ophthalmic drug at their surface. These drug-loaded microparticles first transfer the drug to the concave surface of the contact lens, and when worn, the drug is transferred again, now from the lens to the cornea. This is achieved through the diffusion of the drug from one hydrophobic microenvironment (the silicone moieties of the contact lens polymer network) to another hydrophobic microenvironment (the corneal epithelium) over a short pathway. The second drug transfer was observed and studied in experiments using an ex vivo porcine eye model. The results show that the drug amount that was absorbed by the cornea after applying the rebamipide-loaded contact lenses is approximately 3× (10.7 ± 3.1 μg) as much as the amount of rebamipide that gets transferred after the instillation of one eye drop (1% solution ( p < 0.001 ). The new drug-loading method offers a practical and reproducible means of delivering ophthalmic drugs to the cornea through soft contact lenses. The drug payloads achieved are comparable to dosages used during eye drop therapy.
研究领域:
药学
·
眼科
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
OPTICAL MATERIALS
·
2023
·
IF 3.9
Fan Yang,Sheng-Tong Wu,Xiao-Qiong Lei,Xue-Lian Han,Ping Chen,Shou-Nian Ding
Traditional contact lenses patterns are mostly colored with organic pigments, but they suffered a pigment leakage risk after long-term wearing, which causes corneal inflammation and ulceration. To solve this issue, the structural color is applied to contact lenses because it has color and pigment free. Herein, we reported the application of SiO 2 photonic crystal microspheres in contact lenses. We successfully prepared SiO 2 photonic crystal ink with structural color and fabricated contact lenses by ink transfer printing, and these contact lenses also contain patterns and pupil areas that are the same as the traditional pigment one. In addition, in vitro cytotoxicity test, physicochemical property test, and optical property test were carried out to verify the performance of the prepared structural color lenses. All results suggested it has great potential to replace traditional organic pigment contact lenses.
研究领域:
材料科学
·
生物医学工程
·
眼科
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Advances
·
2022
·
IF 0
Xilin Lin,Jinqiang Liu,Fei Zhou,Yangtao Ou,Jianhua Rong,Jianhao Zhao
Tear protein deposition resistance and antimicrobial property are two challenges of conventional poly(2-hydroxyethyl methacrylate) (pHEMA) contact lenses. In this work, we developed a poly(2-hydroxyethyl methacrylate- co -quaternary ammonium salt chitosan) hydrogel, named as p(HEMA-co-mHACC) hydrogel, using acryloyl HACC (mHACC) as a macromolecular crosslinker. With increasing the acryloyl substitution degree (14–29%) or mHACC content (2–11%), the hydrogel showed an enhanced tensile strength (432–986 kPa) and Young's modulus (360–1158 kPa), a decreased elongation at break (242–84%), and an increased visible light transmittance (0–95%). At an optimal acryloyl substitution degree of 26%, with the increase of mHACC content from 2% to 11%, p(HEMA-co-mHACC) hydrogel presented a decreased water contact angle from 84.6 to 55.3 degree, an increased equilibrium water content from 38% to 45%, and an enhanced oxygen permeability from 8.5 to 13.5 barrer. Due to the enhancement in surface hydrophilicity and electropositivity, p(HEMA-co-mHACC) hydrogel remarkably reduced the deposition of lysozyme, but little affected the adsorption of BSA, depending on the hydrophilic/hydrophobic and electrostatic interactions . The antimicrobial test against Staphylococcus aureus and Escherichia coli showed that p(HEMA-co-mHACC) hydrogel presented an 8–32 times higher germicidal ability than pHEMA hydrogel, indicative of a better antimicrobial activity. The in vitro cell culture of mouse NIH3T3 fibroblasts and immortalized human keratinocytes showed that p(HEMA-co-mHACC) hydrogel was non-toxic. Thus, p(HEMA-co-mHACC) hydrogel with tear protein deposition resistance and antimicrobial activity is a potential candidate for contact lenses.
研究领域:
生物材料学
·
眼科医学
·
高分子化学
✓ 已验证
产品关联
DOI
PubMed
JOURNAL OF CONTROLLED RELEASE
·
2025
·
IF 11.5
Anqi Ye,Hao Mei,Zheng Zhang,Fuqiang Song,Linyuan Jiang,Ting Huang,Ping Li,Siting Du,Yanzhen Feng,Tianyao Jiang,Fenzan Wu,Li Cheng,Jia Qu,Jian Xiao
Severe corneal injuries can cause visual impairment even blindness. Surgically stitching or implanting biomaterials have been developed, but their implementation requires professional surgeons, failing to address the immediate need of medical treatment. The pressing challenge lies in developing multifunctional biomaterials that enable self-management of corneal injuries. This study introduces collagen-based hydrogels that can be used as contact lenses, incorporating macromolecular collagen into common polymer materials via a dual-step orthogonal cross-linking process. This method ensures superior optical and mechanical performance while preserving the bioactivity and structural stability of the incorporated materials. Specialized contact lens facilitates the controlled release of labile protein therapeutics such as acidic fibroblast growth factor (aFGF), eliminating the need for stabilizers like heparin. This capability allows the lens to deliver a wide range of labile proteins, thus expanding its therapeutic use across various ophthalmic and potentially other medical conditions. The lens's anti-inflammatory and anti-fibrotic properties effectively treat corneal alkali burn. Termed ‘corneal first-aids lens’, it can provide postoperative clinical care and serve as a viable and safe therapeutic alternative for patients with limited medical access.
研究领域:
生物医学工程
·
眼科
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2017
·Vol. 17
·期 7
·1600444
·
IF 3.238
Chengfeng Zhang,Ziyuan Liu,Haiye Wang,Xiaofeng Feng,Chunju He
A unique l -cysteine conjugated antifouling amphiphilic conetwork (APCN) is synthesized through end-crosslinking of well-defined triblock copolymers poly(allyl methacrylate)- b -poly(ethylene glycol)- b -poly(allyl methacrylate) via a combination of reversible addition-fragmentation chain transfer (RAFT) polymerization and thiol–ene “click” chemistry. The synthesized poly(ethylene glycol) macro-RAFT agent initiates the polymerization of allyl methacrylate in a controlled manner. The vinyl pendant groups of the precursor partially conjugate with l -cysteine and the rest fully crosslink with mercaptopropyl-containing siloxane via thiol–ene click chemistry under UV irradiation into APCNs, which show distinguished properties, that is, excellent biocompatibility, more than 39.6% water content, 101 barrers oxygen permeability, optimized mechanical properties, and more than 93% visible light transmittance. What's more, the resultant APCNs exhibit eminent resistance to protein adsorption, where the bovine serum albumin and lysozyme adsorption are decreased to 12 and 21 µg cm −2 , respectively. The outstanding properties of APCNs depend on the RAFT controlled method, which precisely designs the hydrophilic/hydrophobic segments and eventually greatly improves the crosslinking efficiency and homogeneity. Meantime, the l -cysteine monolayer can effectively reduce the surface hydrophobicity and prevent protein adsorption, which exhibits the viability for antifouling surface over and under ophthalmic devices, suggesting a promising soft contact lens.
研究领域:
材料科学
·
聚合物化学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Zequn Sun,Fan Li,Kai Shi,Yiyan Gao,Lijie Duan,Guanghui Gao
Despite the significant advancements in hydrogel materials, contemporary hydrogel contact lenses still need to protect against ultraviolet rays. This study explores the impact of silane-modified silica nanoparticles (SMSi nanoparticles) and N-vinylpyrrolidone (NVP) on the properties of hydroxyethyl methacrylate (HEMA) corneal contact lenses. The average diameter of SMSi nanoparticles was 300 ± 100 nm, and in situ copolymerization was employed to create poly(HEMA-NVP-SMSi) hydrogel contact lenses. The enhanced ultraviolet–visible (UV)-absorbing hydrogel materials were analyzed for their structural, optical, and performance properties, including water content, oxygen permeability, and stability. The findings indicated that NVP increased the hydrophilicity of the material while the silica nanoparticles effectively absorbed ultraviolet rays and underwent modifications to reduce aggregation. Moreover, the oxygen permeability of the hydrogel lenses is more than double that of pure pHEMA lenses, demonstrating excellent stability and safety.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Cell Reports Physical Science
·
2024
·
IF 7.9
Yuzhuo Fan,Fanfan Chen,Wanzhong Yuan,Yuchun Sun,Jiarui Li,Yan Li,Mingwei Zhao,Xu Zhang,Kai Wang
Contact lens (CL)-associated bacterial keratitis (BK), a prevalent and underestimated disorder caused by unhygienic CL wear, poses a risk to permanent loss of visual acuity. Clinically, low drug-delivery efficiency, frequent administration, hormone complications, and antibiotic resistance remain the major unsolved challenges. Here, we introduce a chlorogenic acid (CGA)-conjugated CL material based on gelatin methacrylate via cryogelation(cGelMA/CGA-CL) to strengthen the prevention and treatment of BK. The cGelMA/CGA-CL features a highly moist, macroporous, adjustable structure for sustained release of CGA and is favorably biocompatible to cells, providing antimicrobial protection against opportunistic pathogens and inhibiting excessive ocular inflammatory responses through the JAK2-STAT1/STAT2 signaling pathway. Furthermore, the cGelMA/CGA-CL effectively alleviates the symptoms of BK with immunoregulation of macrophage recruitment and anti-inflammatory factor release in a mouse model of BK. The cGelMA/CGA-CL offers a promising candidate for the prevention and treatment of BK, which may significantly reduce the risk of infection for CL wearers.
研究领域:
眼科学
·
生物材料
·
药学
·
免疫学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）