Xuemei Dai,Lu Lin,Yinghai Liu,Libang Yuan,Xuedong Gu,Lingxi Zhu,Wei Wu,Gu Gong
The flexible modification of hollow mesoporous silica nanospheres (HMNs) provides a great opportunity to construct a new generation of multifunctional nanocarriers. In this study, we developed a novel HMNs-based drug delivery system, HA@GHMNs, which is composed of gadolinium-doped hollow mesoporous silica nanospheres (GHMNs) and the targeting molecule hyaluronic acid (HA). A series of physicochemical characterizations, including size, morphology, drug loading capacity, and magnetic properties, confirmed the successful preparation of HA@GHMNs. HA@GHMNs loaded with paclitaxel (PTX), termed HA@GHMNs-PTX, exhibited remarkable antitumor activity. Compared to GHMNs carriers, HA@GHMNs showed negligible toxicity and significantly enhanced cellular uptake in 4T1 cells. Moreover, HA@GHMNs demonstrated a high relaxivity of 16.48 mM −1 s −1 , which is four times higher than that of clinical gadolinium chelates. In vivo experiments further confirmed that HA@GHMNs-PTX effectively inhibited tumor growth and enabled magnetic resonance imaging (MRI) visualization of the tumor site. Overall, HA@GHMNs-PTX shows great potential as an efficient theranostic nanoplatform for tumor treatment and MRI.
Ying Liu,Yuxia Tang,Ying Tian,Jiang Wu,Jing Sun,Zhaogang Teng,Shouju Wang,Guangming Lu
Hydroxyapatite nanoparticles have drawn much attention in the development of multifunctional nanosystems because of their excellent biocompatibility. In the report, gadolinium-doped hydroxyapatite nanorods (NHA:Gd) were controlled synthesized through adjusting the amount of gadolinium. The NHA:Gd showed a high loading capacity for doxorubicin (118 μg per milligram) and a pH-responsive drug release ability. The r1 value of obtained doxorubicin (DOX)-loaded NHA:Gd (NHA:Gd-DOX) solution reached 0.0472 (μg/mL)−1 s–1 and was higher than that of commercial T1 contrast Gd-DTPA (0.0314 (μg/mL)−1 s–1). In vivo experiments demonstrated that NHA:Gd-DOX exhibited excellent MR imaging property. The results of therapeutic efficacy demonstrate that the tumor volume treated by NHA:Gd-DOX was significantly reduced compared to that treated by free DOX. In vivo toxicity experiments demonstrated no obvious damage to the major organs in NHA:Gd-DOX treated mice. Considering the excellent biocompatibility, T1WI contrast ability, and enhanced therapeutic efficacy, these NHA:Gd-DOX have great potential for applications as MR T1 contrast agents and drug carriers for cancer therapy.
It is a still tough task to precisely target cancer cells and efficiently improve the therapeutic efficacy of various therapies at the same time. Here, dual-template imprinting polymer nanoparticles (MIPs) with a core–shell structure were prepared, in which fluorescent silica nanoparticles (FSiO2) were the core and the imprinted polymer layers were the outermost shell. The imprinted layer was designed and constructed via free-radical precipitation approach on the surface of FSiO2, which simultaneously encapsulated gadolinium-doped silicon quantum dots and photosensitizers (Ce6). During the polymerization process, two template molecules were introduced into the mixtures, one was the epitope of CD59 protein (YNCPNPTADCK), which was overexpressed on the surface of a great deal of the solid cancers, and the other was antitumor agent doxorubicin (DOX) to be used for chemotherapy. Furthermore, the embedded Ce6 could generate toxic 1O2 under 655 nm laser irradiation to kill cancer cells, combining with the loaded-DOX to obtain a synergistic cancer therapy. Moreover, owing to the introduction of gadolinium-doped silicon quantum dots, Ce6, and DOX, the MIPs were endowed with targeted fluorescence imaging (FI) and MR imaging (MRI). In vitro and in vivo experiments had been conducted to demonstrate the excellent targeting ability and desirable treatment effect with negligible toxicity to healthy tissues and organs. As a consequence, the designed MIPs can promote the development of targeted recognition against biomarkers and precise treatment guided with cell imaging tools.
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