Hongtao Du,Juncheng Chen,Shan Tian,Hongling Gu,Na Li,Yao Sun,Jiajia Ru,Junru Wang
In this research, extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp were investigated. A response surface methodology (RSM) with a Box-Behnken design (BBD) was used to optimize the extraction process. The maximum EAP yield was 9.82 ± 0.38%, which is in good agreement with the predicted value (9.93 ± 0.24%). Two homogeneous polysaccharides, EAP-1a and EAP-1b with molecular weights of 8.70 kDa and 4.39 kDa respectively, were prepared by DEAE-52 cellulose and Sephadex G-100 columns and characterized by HPLC, HPGPC, and FT-IR. Three polysaccharides (EAP, EAP-1a and EAP-1b) could stimulate macrophages to release NO and enhance phagocytic activities of RAW 264.7 cells in dose-dependent manner. Moreover, there was no significant difference between crude EAP group (400 μg/mL) and positive control group (LPS) in effects on macrophages. The results implied that EAP had the potential to be developed as natural medicines or health foods .
Zhongxu Li,Ruiguo Cui,Weiwei Liu,Mengshi Wang,Lilang Li,Fengjuan Liu,Bin Du,Lijun Song
The wine-making industry produces a large amount of grape pomace, which is rich in anthocyanins. To avoid wasting these resources, choline chloride: lactic acid (molar ratio of 1:2) (DES-1) was selected as the optimal solvent. The optimal extraction conditions were as follows: temperature 60°C, time 60 min, water content 25%. The anthocyanins yield was 5.73 mg (cyanidin-3-glucoside) equivalent/ g under the optimal conditions, which was significantly higher than that obtained by DES-3 (Choline chloride: 1,4 butanediol), DES-5 (Choline chloride: 1,2 propylene glycol), and ethanol. The DES-1 extract showed significant higher color stability and antioxidant activities compared with the anthocyanins extracted by DES-3, DES-5, and ethanol. A total of eight individual anthocyanin compounds were identified in each extract, with delphinidin 3-O-glucoside, peonidin 3-O- glucoside, petunidin, and delphinidin as the dominant compounds. Molecular dynamics simulation confirmed that DES-1 system showed a larger average number of hydrogen bonds (11.63), average lifetime of hydrogen bonds (364.21 ps) and with a reduced total interaction energy (-706.82 kJ/moL), thus improving the extraction efficiency and stability of anthocyanins. This study would provide new protocols for the extraction of anthocyanins from grape pomace.
危险品化学品经营许可证（带存储）