Frontiers in Nutrition
·
2023
·
IF 5
Ma Yinpeng,Zhang Piqi,Dai Xiaodong,Yao Xiuge,Zhou Shuyang,Ma Qingfang,Liu Jianing,Tian Shuang,Zhu Jianan,Zhang Jiechi,Kong Xianghui,Bao Yihong
Introduction: Natural melanin from Auricularia heimuer have numerous beneficial biological properties, which were used as a safe and healthy colorant in several industries.Methods: In this study, single-factor experiments, Box-Behnken design (BBD), and response surface methodology (RSM) were employed to investigate the effects of alkali-soluble pH, acid precipitation pH, and microwave time on the extraction yield of Auricularia heimuer melanin (AHM) from fermentation. Ultraviolet-visible spectrum (UV-Vis), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), and high-performance liquid chromatography (HPLC) were used to analyze the extracted AHM. The solubility, stability, and antioxidant activities of AHM were also measured.Results: The results showed that alkali-soluble pH, acid precipitation pH, and microwave time significantly affected the AHM yield, with the following optimized microwave-assisted extraction conditions: alkali-soluble pH of 12.3, acid precipitation pH of 3.1, and microwave time of 53 min, resulting in an AHM extraction yield of 0.4042%. AHM exhibited a strong absorption at 210 nm, similar to melanin from other sources. FT-IR spectroscopy also revealed that AHM exhibited the three characteristic absorption peaks of natural melanin. The HPLC chromatogram profile of AHM showed a single symmetrical elution peak with a 2.435 min retention time. AHM was highly soluble in alkali solution, insoluble in distilled water and organic solvents, and demonstrated strong DPPH, OH, and ABTS free radical scavenging activities.Discussion: This study provides technical support to optimize AHM extraction for use in the medical and food industries.
研究领域:
食品科学
·
生物化学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYTOCHEMICAL ANALYSIS
·
2021
·Vol. 32
·期 6
·1074-1081
·
IF 3.373
Yanfang Wu,Yindi Chu,Qinyu Yang,Mengjia Li,Miao Yu,Ruixue Deng,Kun Liu,Xinsheng Wang,Enguo Fan
Introduction Syringa pubescens Turcz. was reported to be abundant in the Funiu Mountains of Henan Province and can be used to treat hepatitis and cirrhosis. In order to develop and utilise the resource, a fast and simple technique to extract bioactive compounds is needed. Objectives Our aims were to provide an extraction technique of glycosides from S. pubescens and study the antioxidant activity of this material. Methods Box–Behnken design (BBD) was employed with three factors at three levels. The process parameters such as ethanol concentration ( X 1 ), temperature ( X 2 ), and solvent–solid ratio ( X 3 ) could significantly influence efficiency and yield of target compounds. High-performance liquid chromatography (HPLC) was used to determine the content of glycosides. DPPH (α,α-diphenyl-β-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and reducing power were used to evaluate the antioxidant activity of S. pubescens extract. Results The optimal parameters for the maximal extraction yield were obtained with ethanol concentration of 68%, temperature of 89°C, solvent–solid ratio of 46 mL/g, and time of 20 min. The S. pubescens extract showed strong antioxidant properties in vitro . Conclusion The findings indicated the potential application of solvothermal extraction method to extract bioactive compounds from S. pubescens Turcz. Furthermore, the S. pubescens extract could be used as an important resource of antioxidant activity.
研究领域:
植物化学
·
药用植物学
·
天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CATALYSIS LETTERS
·
2022
·Vol. 153
·期 4
·1096-1108
·
IF 2.936
Tang Lingqian,Fan Mingxuan,Pan Zhiquan,Cheng Qingrong,Feng Lu,Wu Hanjun,Zhou Hong
Low-molecular weight solid acid, [(DETA-(SO 3 H) 2 ], was synthesized and fully characterized by FTIR, XRD, TGA, XPS, 1 H-NMR and elemental analysis. The prepared solid acid exhibited high strong acid density (7.6 mmol H + /g), much higher than most of other solid acids. The solid acid was used for the extraction of diosgenin from the saponins in Dioscorea zingiberensis C. H. Wright. Diosgenin is a very medical intermediate for the synthesis of various kinds of steroidal drugs. To improve the diosgenin yield, the optimal conditions of the extraction reaction were investigated by single-factor experiments. Under the optimized conditions, the diosgenin yield catalyzed by the solid acid was much higher than those obtained by the catalysis of other solid acids or conventional sulfuric acid. Importantly, no waste liquid was discharged in the approach as the reaction medium can be recycled. The alcoholysis approach for the production of diosgenin has a great potential application in the industry. Graphical Abstract A novel low-molecular weight solid acid was synthesized and fully characterized. The solid acid showed excellent catalytic activity for the catalytic alcoholysis of saponins from D. zingiberensis C. H. Wright (DZW), the catalyst can be recycled and the whole extraction process is green and efficient.
研究领域:
化学工程
·
植物化学
·
医药工业
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Tang Liqin,Liu Haocheng,Wen Jing,Xu Yujuan,Tian Wenni,Li Lu,Yu Yuanshan,Lin Xian,Fu Manqin
The acquisition of yellow extract (YE) from gardenia ( Gardenia jasminoides ) fruit can promote the application of natural pigment. This study aims to establish an effective method for extracting YE from gardenia fruit and to evaluate the properties of YE. First, the seven compounds in YE obtained by ultrahigh-pressure extraction (UPE) were identified by LC-TOF-MS/MS. The effects of traditional extraction (TE), ultrasonic extraction (UE), and UPE methods on the yield of YE were then investigated. The results showed that the extraction yield of UPE was 7.73 ± 0.89 mg/g DW under the optimized conditions (47.78% ethanol concentration, 114.95 s time, and 18.08 mL/g liquid–solid ratio), which was higher than those of UE (4.81 ± 0.23 mg/g DW) and TE (3.55 ± 0.21 mg/g DW). Finally, the YE stability of UPE was studied. The results showed that the YE stability of UPE was similar to that of the TE group, and both of which were greatly affected by higher temperature (60–100 °C), ultraviolet radiation, alkalinity , and Fe 3+ . Meanwhile, YE of UPE had better stability under ultraviolet radiation and alkalinity in comparison with the TE group. Therefore, UPE is a potential method for YE extraction from gardenia fruit.
研究领域:
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Zhenlei Wang,Wenqi Cheng,Zitong Wang,Yanhui Wen,Jing Bai,Hongli Zhou,Yahong Wang
This study was for the preparation of bioactive non-starch polysaccharides from nutmeg (NEP), addressing the high content of lipids and starch in nutmeg. First, lipids were removed by 3 times defatted with petroleum ether. Then, the optimal extraction process was 170 min at 82 °C with a liquid solid ratio of 15: 1 mL/g. Finally, the removal of starch was confirmed through enzymatic hydrolysis with α-amylase and amyloglucosidase, supported by I 2 -KI reagent, HPLC, and IR analysis. The yield of NEP was 3.09 %. Compared with non-enzymatic hydrolysis polysaccharides (NNP), total sugar and uronic acid contents were increased by 13.8 % and 18.5 %, respectively. The molecular weight decreased from 1.031 × 10 6 to 6.31 × 10 5 Da, exhibiting a porous sheet-like morphology and β-glycosidic bonds. NEP had superior activity, with an IC 50 value of 0.47 mg/mL for ABTS radical scavenging, compared to 1.20 mg/mL for NNP, and its total reducing capacity was nearly double that of NNP. 100 μg/mL of NEP stimulated RAW264.7 macrophages to release 12.14 μM of nitric oxide, exceeding the 9.13 μM induced by lipopolysaccharide. These results indicate the antioxidant and immunomodulatory efficacy of NEP, highlighting its potential as an active ingredient in foods or pharmaceuticals.
研究领域:
食品科学
·
药理学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Oleo Science
·
2023
·
IF 1.5
Yun Liu,Shuai Zhu,Xiazhong Liu
In this study, four acetone-ethanol protocols were employed to investigate the effect of extraction processes on the yield and purity of phosphatidylcholine (PC) from dried egg yolk powder and fresh liquid egg yolk, as well as the cholesterol distribution between the oil and PC fraction. Furthermore, the physicochemical (thermo-stability, fatty acid composition, and molecular structure) and emulsifying (zeta potential, particle size, EAI, ESI, and creaming index) properties of the final PC product were also examined. In addition, the structural characteristics of the egg yolk residual protein were highlighted to promote its application in food industries. The results showed that de-oiling with acetone prior to ethanol extraction can achieve high yield (19.92%) and purity (68.62%) of the PC product with low cholesterol content (< 0.12%). The extraction processes exhibited a significant impact on the emulsifying properties of the PC product. The creaming index of PC emulsions was higher than that of egg yolk powder emulsions with high protein concentration, suggesting that PC plays a critical role in the emulsifying stability of egg yolk protein dispersion. The structural characteristics of residual protein, including free sulfhydryl groups and primary, secondary, and ternary structures, showed considerable differentiation related to extraction processes. These findings provide a powerful tool for the dietary utilization of egg yolk PC and protein in future.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS One
·
2015
·Vol. 10
·期 5
·e0128236
·
IF 3.234
Xingchu Gong,Yao Li,Huali Chen,Haibin Qu
A design space approach was applied to optimize the extraction process of Danhong injection. Dry matter yield and the yields of five active ingredients were selected as process critical quality attributes (CQAs). Extraction number, extraction time, and the mass ratio of water and material (W/M ratio) were selected as critical process parameters (CPPs). Quadratic models between CPPs and CQAs were developed with determination coefficients higher than 0.94. Active ingredient yields and dry matter yield increased as the extraction number increased. Monte-Carlo simulation with models established using a stepwise regression method was applied to calculate the probability-based design space. Step length showed little effect on the calculation results. Higher simulation number led to results with lower dispersion. Data generated in a Monte Carlo simulation following a normal distribution led to a design space with a smaller size. An optimized calculation condition was obtained with 10000 simulation times, 0.01 calculation step length, a significance level value of 0.35 for adding or removing terms in a stepwise regression, and a normal distribution for data generation. The design space with a probability higher than 0.95 to attain the CQA criteria was calculated and verified successfully. Normal operating ranges of 8.2-10 g/g of W/M ratio, 1.25-1.63 h of extraction time, and two extractions were recommended. The optimized calculation conditions can conveniently be used in design space development for other pharmaceutical processes.
研究领域:
中药学
·
制药工程
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Wenqi Liu,Ying Sun,Yansheng Zhao,Fengxiang Liang,Yunxiang Que,Jing Yang,Lili Guan
The purpose of this study was to valorize safflower post-extraction residue by comparing the structure characterization, function characteristics, and bioactivity of four safflower polysaccharides (SPSs: HWES, UAES, HES, AES) extracted via different procedures. AES showed the highest yield (1.59 ± 0.01 %) and polysaccharides content (92.07 ± 0.09 %). SPSs had molecular weights of 256.46–404.38 kDa, UAES had the best water holding capacity (2.55 ± 0.14 g/g), HES had the best foaming stability (82.89 ± 0.38 %). SPSs all exhibited shear-thinning behavior, with HES showing the highest viscosity and prominent viscous liquid-like characteristics. AES had small particle size and high thermal stability and the best foaming capacity (214.52 ± 8.74 %) and emulsification activity index (5.14 ± 0.04 m 2 /g), and displayed optimal antioxidant activity and immunomodulatory effect. These findings established the isolation procedures for safflower post-extraction residue polysaccharides, identified AE procedure as the most viable approach for producing industrially relevant bioactive SPSs, and provided a practical strategy for enhancing the utilization of safflower residue.
研究领域:
食品科学
·
天然产物化学
·
生物大分子
·
功能食品
·
多糖化学
·
生物活性物质
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2019
·
IF 5.839
Guangjing Chen,Xuhui Chen,Bing Yang,Qingqing Yu,Xunyu Wei,Yongbo Ding,Jianquan Kan
The in vitro digestibility, prebiotic activity, and physicochemical properties of polysaccharides obtained from bamboo shoots ( Chimonobambusa quadrangularis ) using different extraction processes were investigated in this study. Five polysaccharide fractions were prepared by hot water-, accelerated solvent-, ultrasound-, microwave-, and enzyme-based extraction processes. First, the chemical and monosaccharide compositions, molecular weights, zeta potentials , and particle sizes of the five fractions were assessed using colorimetry , high-performance liquid chromatography, high-performance gel permeation chromatography , and dynamic light scattering. Next, digestibility was tested using artificial gastric juice and α-amylase. Finally, the five polysaccharide fractions were added to liquid cultures of bifidobacteria and lactobacilli strains to serve as carbon sources alternative to glucose for in vitro fermentation to evaluate their ability to promote the growth of probiotic bacterial species and stimulate short chain fatty acids (SCFAs) production. It was found polysaccharides obtained by ultrasound- and enzyme-assisted extraction had significantly ( p < 0.05) higher total carbohydrate contents of 61.9% and 61.5%, lower medium-high MWs of 117.49 and 117.25 kDa, and smaller particle sizes of 116.8 and 190.73 nm, respectively. All of the polysaccharide fractions were resistant to hydrolysis by artificial gastric juice and α-amylase. Furthermore, the five polysaccharides significantly stimulated the growth of the tested probiotics and increased lactic, acetate, propionate , and butyrate acids production from fermentation for 48 h compared to the control ( p < 0.05). The polysaccharides prepared using ultrasound- and enzyme-assisted extraction processes displayed better prebiotic activity than the other fractions as they induced notably more proliferation of probiotic bacteria and higher production of SCFAs.
研究领域:
食品科学
·
营养学
·
微生物学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Plant Science
·
2022
·
IF 6.627
Zhang Jianyun,Zhao Ziyi,Liang Wenyu,Bi Jingyi,Zheng Yuguang,Gu Xian,Fang Huiyong
Sabina chinensis is a woody plant with important ecological functions in different regions of China, but its essential oils (EO) against plant pathogenic fungi remain largely undetermined. The purpose of our study was to assess the chemical composition and antifungal activity of S. chinensis EO based on optimization of the extraction process. In this study, an actionable and effective model with the experimental results and identified optimum conditions (crushing degree of 20 mesh, liquid–solid ratio of 10.1:1, immersion time of 9.1 h) was established successfully to achieve an extraction yield of 0.54%, which was basically consistent with the theoretical value. A total of 26 compounds were identified using headspace gas chromatography–mass spectrometry (GC–MS) and showed that the major constituent was β-phellandrene (26.64-39.26%), followed by terpinen-4-ol (6.53-11.89%), bornyl acetate (6.13-10.53%), etc. For Petri plate assays, our experiments found for the first time that S. chinensis EO revealed high and long-term antifungal activity against the tested strains, including Fusarium oxysporum and Fusarium incarnatum, at EC50 values of 1.42 and 1.15 µL/mL, which especially reached approximately 76% and 90% growth inhibition at a dose of 0.2 µL/mL, respectively. Furthermore, the antifungal activity of EO from different harvest periods showed remarkable variation. The orthogonal partial least-squares discriminant analysis (OPLS-DA) method revealed 11 metabolites with chemical marker components, and 5 of its potential antifungal activities, terpinen-4-ol, α-terpineol, α-elemol, γ-eudesmol, and bornyl acetate, were strongly correlated with the mycelial inhibition rate. In total, this study explored the antifungal activity of EO against root rot fungus as a potential fungicide and provided valuable information into developing potential products from natural agents.
研究领域:
植物科学
·
植物病理学
·
天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Junjie Qiu,Xiao Xu,Jingyan Guo,Zhenyu Wang,Jinjin Wu,Huiqin Ding,Yuchen Xu,Yili Wu,Qianyi Ying,Jiawei Qiu,Suxiang Wu,Senlin Shi
We optimized the extraction process of Bletilla striata polysaccharides using orthogonal design, Box-Behnken design (BBD), and genetic algorithm-back propagation (GA-BP), then compared and evaluated them to confirm that the combination of BBD and GA-BP neural networks was capable of increasing polysaccharide yields and antioxidant activity. The optimal extraction parameters were as follows: liquid-to-solid ratio of 15 mL/g, extraction power of 450 W, and extraction time of 34 min. Under these conditions, the polysaccharide yield and antioxidant activity were 8.29 ± 0.50 % and 26.20 ± 0.28 (mM FE/mg). Subsequently, the polysaccharide was purified to obtain purified Bletilla striata polysaccharides 1 (pBSP1) with a Mw of 255.172 kDa. Scanning electron microscope (SEM), ultraviolet-visible detector (UV), fourier transform infrared spectrometer (FTIR), high performance liquid chromatography (HPLC), X-ray diffraction (XRD), nuclear magnetic resonance (NMR) and periodate oxidation were used to analyze the structure of pBSP1. The results showed pBSP1 had a smooth surface and a rough interior, with a composition of α-D conformation glucose (18.23 %) and β-D conformation mannose (53.77 %), and an amorphous crystal structure. According to the results of thermogravimetric and rheological tests, pBSP1 exhibits good thermal stability and viscoelastic behavior. Furthermore, pBSP1 protected lipopolysaccharide (LPS)-induced GES − 1 and Caco2 cells, the results showed pBSP1(400 μg/mL) lowered TEER synthesis in Caco2 cells as well as apoptosis and reactive oxygen species (ROS) production in both cells, indicating that pBSP1 may have an intestine protective effect.
研究领域:
药理学
·
生物化学
·
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Pharmaceuticals
·
2025
·Vol. 18
·期 9
·1272
·
IF 4.8
Jie Li,Baojian Li,Xinbo Shi,Yuangui Yang,Zhongxing Song
Background/Objectives: As a non-medicinal part resource ofZiziphus jujuba, this study focuses on the total flavonoids fromZiziphus jujubamesocarp (TFZJM), aiming to optimize the extraction process and explore its sedative and hypnotic effects.Methods: The extraction process of TFZJM was optimized by using single-factor experiments and the Box-Behnken response surface design method. The material basis of TFZJM was analyzed using Ultra-Performance Liquid Chromatography-Quadrupole-Time of Flight-Mass Spectrometry (UPLC-Q-TOF-MS). The mouse insomnia model was induced by intraperitoneal injection of PCPA, and the effects of TFZJM on this model and its potential mechanism were evaluated using multiple methods, such as sleep enhancement induced by pentobarbital sodium, HE staining of tissue sections, ELISA, RT-PCR, WB, and serum metabolomics.Results: The results showed that by optimizing the extraction conditions, a solid-liquid ratio (SLR) of 1:25 g·mL−1, ethanol concentration of 60%, extraction time of 60 min, and extraction rate of 1.98% were achieved. The common chemical basis of the 10 flavonoid components was identified using UPLC-Q-TOF-MS analysis. Compared with the model group, the high-dose TFZJM (TFZJM-H) group had the most significant effect, followed by the medium-dose (TFZJM-M) and low-dose (TFZJM-L) groups.Conclusions: Metabolomic analysis revealed that TFZJM regulates pathways related to the metabolism of phenylalanine, tyrosine, cytochrome P450, and alanine. This lays the foundation for further exploration of the active substances and mechanisms of action of TFZJM in sedation and hypnosis.
研究领域:
生药学
·
天然产物化学
·
神经药理学
·
代谢组学
·
中医药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）