SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Hui Lin,Yinqiu Yuan,Xiaoman Jiang,Jian-Ping Zou,Xinghui Xia,Shenglian Luo
Microplastics (MPs) are the significant environmental factor for bioavailability of hydrophobic organic contaminants (HOCs) in aquatic environments. Nevertheless, the bioavailability of microplastic-associated HOCs remains unclear. In this research, the freely dissolved pyrene concentrations were kept stable with passive dosing devices, and the pyrene content in D. magna tissues as well as D. magna immobilization were analyzed to quantify bioavailability of pyrene (a representative HOC) associated with naturally-aged polystyrene (PS) MPs. Furthermore, the uptake mechanisms of pyrene associated with MPs of different sizes were explored by investigating the distribution of MPs in D. magna tissues with scanning electron microscopy. Especially, a new schematic model of bioavailability process was established. The results demonstrated that a part of pyrene associated with 0–1.5 μm MPs could directly cross cell membrane through endocytosis from intestine and exposure solutions to D. magna tissues except the 10–60 and 60–230 μm MPs. The bioavailability of microplastic-associated pyrene was ordered as 0–1.5 μm (20.0–21.6%) > 10–60 μm (10.7–13.8%) > 60–230 μm MPs (6.0–9.8%), which were essentially resulted from the difference in uptake mechanisms of pyrene associated with MPs of different sizes. This work suggests that the bioavailability of microplastic-associated HOCs should be considered when assessing water quality and environmental risk of HOCs in natural waters.
研究领域:
环境科学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Gongxi Qiao,Lei Liu,Xiaoxing Hao,Junke Zheng,Wanqiang Liu,Jinwei Gao,Cheng Cheng Zhang,Qianming Wang
As the standard for heavy metal sensing systems increases, it becomes more important to find out new self-indicating materials as alternatives. The traditional design of optical probe contains receptor and fluorophore as two moieties. Here a novel sulfur quantum dot as the intrinsic fluorescent sensor has been established and the signal transduction involves interaction of mercury ions with sulfur atoms which belongs to the internal system of the fluorophore. Its analytical performances can be assessed by fluorometric and colorimetric titrations simultaneously and the detection limits for Hg 2+ ions are determined to be 65 nM and 1.86 μM respectively. In the presence of sulfur dots, very low cytotoxicity has been verified in two cell lines (HeLa and K562) via both cell counting kit-8 method and flow cytometry. For the first time, four sorts of chemical inhibitors have been employed to clarify the endocytosis mechanism based on sulfur quantum dots. The collected results indicate that clathrin and lipid raft mediated endocytosis play as primary roles in the internalization processes. It has been found that sulfur dots are located in the cytoplasm with intense blue emissions and the cellular tracking of mercury ions has been realized.
研究领域:
分析化学
·
生物医学工程
·
毒理学
✓ 已验证
DOI
高影响因子
ANALYST
·
2016
·Vol. 141
·期 11
·3337-3342
·
IF 4.033
Shanshan Wang,Zhangkun Liu,Yuxiu Zou,Xiaofang Lai,Ding Ding,Long Chen,Liqin Zhang,Yuan Wu,Zhuo Chen,Weihong Tan
Elucidating the endocytosis and metabolism of nanoparticles in cells could improve the diagnostic sensitivity and therapeutic efficiency. In this work, we explore the cellular uptake mechanism of a biocompatible nanocrystal nanostructure, graphene-isolated-Au-nanocrystals (GIANs), by monitoring the intrinsic Raman and two-photon luminescence signals of GIANs in live cells. Aptamers functionalized on the GIAN nanostructure through simple, but strong, π–π interactions entered the cells through a clathrin-dependent pathway, while unmodified GIANs mainly entered the cells through a caveolae-mediated endocytosis pathway. Thus, it can be concluded that the mechanism of cellular uptake in these graphene-isolated-Au-nanocrystal nanostructures is determined by the presence or absence of aptamer modification.
研究领域:
生物医学工程
·
纳米医学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2017
·
IF 4.3
Huibo Chi,Yan Gu,Tingting Xu,Feng Cao
To study the cellular uptake mechanism of multifunctional organic–inorganic hybrid nanoparticles and nanosheets, new chitosan–glutathione–valine–valine-layered double hydroxide (CG-VV-LDH) nanosheets with active targeting to peptide transporter-1 (PepT-1) were prepared, characterized and further compared with CG-VV-LDH nanoparticles. Both organic–inorganic hybrid nanoparticles and nanosheets showed a sustained release in vitro and prolonged precorneal retention time in vivo, but CG-VV-LDH nanoparticles showed superior permeability in the isolated cornea of rabbits than CG-VV-LDH nanosheets. Furthermore, results of cellular uptake on human corneal epithelial primary cells (HCEpiC) and retinal pigment epithelial (ARPE-19) cells indicated that both clathrin-mediated endocytosis and active transport of PepT-1 are involved in the internalization of CG-VV-LDH nanoparticles and CG-VV-LDH nanosheets. In summary, the CG-VV-LDH nanoparticle may be a promising carrier as a topical ocular drug delivery system for the treatment of ocular diseases of mid-posterior segments, while the CG-VV-LDH nanosheet may be suitable for the treatment of ocular surface diseases.
研究领域:
药学
·
纳米技术
·
眼科学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）