FISHERIES SCIENCE
·
2022
·Vol. 88
·期 1
·83-90
·
IF 2.148
Feng Hexiong,Peng Di,Liang Xu-Fang,Li Jiao,Luo Haocan,Tang Shulin,Chai Farui
There are unique hypothalamic fatty acid (FA) sensing systems in fish that are different from those in mammals. In order to explore the effect of medium-chain FA (MCFA) on the FA sensing systems and appetite regulation, Chinese perch Siniperca chuatsi were subjected to intracerebroventricular (ICV) injection with saline solution alone or saline containing octanoic acid. Food intake of these fish was computed at 2, 4, 6, 12 and 24 h post-treatment, respectively. The transcript levels of genes related to the FA sensing mechanism ( cpt1c , cd36 , pparα and srebp1c ) and hypothalamic neuropeptide ( agrp , npy , pomc and cart ) in the hypothalamus were estimated at 4 h post-treatment. Results showed that at 4 h post-ICV injection with octanoic acid, the food intake of Chinese perch was significantly reduced with the decreasing mRNA level of agrp and the increasing mRNA levels of cd36 and pparα in the hypothalamus. The data indicated that the hypothalamic FA sensing systems of Chinese perch could be activated by octanoic acid, and the underlying mechanism might be that the enhancement of binding ability of FAT/CD36 upregulates the mRNA level of pparα . In addition, with the decrease in the gene expression of agrp , the food intake of Chinese perch was significantly reduced as a result of ICV injection with octanoic acid. These results demonstrated that MCFA might play a key role in central FA sensing and appetite regulation of Chinese perch.
研究领域:
神经生物学
·
水产养殖
·
分子生物学
✓ 已验证
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2025
·
IF 8
Xiaoer Wu,Ying Yang,Shuang Xie,Ping Zhong,Ning Xu
Harmful algal blooms formed by the mixotrophic dinoflagellate Akashiwo sanguinea are toxic to multiple aquatic organisms, although the relationship between toxicity and grazing is unknown. In this study, the effects of irradiance and nutrient levels on the grazing rate of A. sanguinea on the cryptophyte Rhodomonas salina were investigated using an optimized stable isotope method. Under light-limited conditions (0–30 μmol photons m −2 s −1 ), A. sanguinea exhibited higher hemolytic activity and grazing rate. Under the same level of irradiance, the growth and respiration rates were higher in co-culture treatments, while the oxygen release rate was higher in monoculture treatments. The growth rate, hemolytic activity, and grazing rate of A. sanguinea increased significantly with higher nutrient levels or an unbalanced N:P ratio. The hemolytic activity and grazing rate were higher in urea treatment, while the growth rate was higher in ammonia treatment. This study suggests that toxicity is involved in the feeding process of the mixotrophic dinoflagellate A. sanguinea ; the unique feeding flexibility adapting to low irradiance, increased nutrients, unbalanced N:P ratio, and organic nitrogen urea gains an advantage in interspecific competition among phytoplankton.
研究领域:
海洋生物学
·
生态毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Benshui Shu,Yuting Huang,Xinyi Xie,Cuiting Liu,Jintian Lin,Ying Xiao,Jingjing Zhang
Azadirachtin, a highly effective botanical pesticide, demonstrated notable biological activities against Spodoptera frugiperda , including mortality induction, growth and development inhibition, and antifeedant effects. Neuropeptide F (NPF) has been shown to play a role in various physiological processes in insects. Nonetheless, the functions of Sf- NPF1 in regulating food intake and antifeedant induction by azadirachtin in S. frugiperda larvae are yet to be elucidated. In this study, the antifeedant effects induced by 0.5 and 1.0 μg/g azadirachtin treatments in S. frugiperda larvae were evaluated. The sequence of Sf-NPF1 was identified with two transcript variants, and the proteins exhibit high identity with NPFs found in lepidopteran insects. Spatiotemporal expression analysis indicated that Sf-NPF1 exhibited high expression levels in first instar larvae and male adults, while the lowest expression was observed in non-feeding stages (egg and pupa stages). Besides, Sf-NPF1 also demonstrated notable expression in the midgut tissue of larvae. Azadirachtin treatments inhibited the expression of Sf-NPF1 in the larval midgut. The injection of the Sf- NPF1a mature peptide resulted in increased larval food intake and weight gain during a two-day treatment period. The antifeedant effect induced by 1.0 μg/g azadirachtin was also mitigated by the injection of Sf- NPF1a mature peptide. Our results indicated that Sf- NPF1 may serve as a positive regulator of food intake in S. frugiperda larvae, while azadirachtin could exert an antifeedant effect in larvae via inhibiting the expression of Sf-NPF1 . These findings establish a foundation for comprehending the mechanism behind the antifeedant effect induced by azadirachtin in insect larvae and provide a potential target for developing novel insecticides.
研究领域:
昆虫学
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分子生物学
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农药科学
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生理学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 6
·5704-5712
·
IF 2.914
Nkoom Matthew,Lu Guanghua,Liu Jianchao,Dong Huike,Yang Haohan
The non-steroidal anti-inflammatory drug (NSAID) diclofenac is one of the most frequently studied as well as controversially discussed pharmaceutically active drug on the subject of its relevance to the environment. This study was conducted to assess the bioconcentration potential of diclofenac and its behavioral and biochemical effects in Daphnia magna . The bioconcentration factors of diclofenac determined after 48 h of aqueous exposure in Daphnia magna were 70.94 and 8.02 for the nominal exposure concentrations of 5 and 100 μg/L, respectively. Diclofenac exposure obviously decreased the filtration and ingestion rates of the daphnids. A significant increase of the acetylcholinesterase activity that was observed in this study indicates that diclofenac might not have neurobehavioral toxicity in Daphnia magna . Significant induction of malondialdehyde content is an indication of overproduction of reactive oxygen species leading to oxidative damage in daphnids after diclofenac exposure. Moreover, significant inhibition of the superoxide dismutase, catalase, and glutathione reductase activities implies that the antioxidant defense system of Daphnia magna was overwhelmed. Also, significant inhibition of glutathione s-transferase activity might point to the fact that the enzyme was not capable to detoxify diclofenac in Daphnia magna . These findings indicate that diclofenac can accumulate and consequently stimulate behavioral and biochemical disturbances in Daphnia magna .
研究领域:
环境毒理学
·
水生生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 103
·期 8
·3840-3849
·
IF 4.125
Ying You,Haoran Song,Linlin Wang,Zhengqi Liu,Xiaoming Guo,Chunqing Ai,Shuang Song,Beiwei Zhu
BACKGROUND Caulerpa lentillifera has received extensive attention regarding expansion of its farming and increasing consumption. In our previous study, the structure of C. lentillifera polysaccharide (CLP) was elucidated. However, little information is available about its health effects. In this study, the anti-obesity effect of CLP was investigated by using a high-fat diet-induced obese mice model with two different supplementation methods. RESULTS In vitro simulated digestion results showed that CLP significantly decreased the lipid digestibility and induced the lipid droplets aggregation in the intestinal stage to inhibit the absorption of lipids. As revealed by 16S ribosomal RNA sequencing and non-targeted metabolomics, supplement of CLP by both pre-prandial gavage and free feeding patterns effectively prevented mice obesity via ameliorating intestinal flora disturbance and regulating bile acids circulation metabolism. Of note was that CLP administration had no effect on short-chain fatty acids production, suggesting the anti-obesity effect was uncorrelated with their production. Moreover, pre-prandial administration of CLP had a better anti-obesity effect in lowering body weight and serum lipid levels, but the free feeding resulted in a higher α -diversity of gut microbiota. CONCLUSION The findings of this study indicate that CLP could be a potential anti-obesity nutraceutical and that pre-prandial supplement of CLP may be a better intake method to exhibit its hypolipidemic effect. © 2022 Society of Chemical Industry.
研究领域:
营养学
·
药理学
·
微生物学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE RESEARCH
·
2020
·Vol. 51
·期 3
·1012-1025
·
IF 1.748
Xiao Yan,Chaobin Qin,Dapeng Deng,Guokun Yang,Junchang Feng,Ronghua Lu,Liping Yang,Xiaolin Meng,Xianglin Cao,Guoxing Nie,Guiqin Wang
Apelin and its receptor apj have a variety of biological functions and are involved in appetite and energy regulation. However, the comparative studies of apelin/apj system in fish are still absent. In this study, the open reading frames of apelin and apja and apjb were obtained. Apelin and apj mRNAs were widely distributed in all tissues detected, with the highest level in the brain, followed by the spleen, kidney and foregut. The mRNA expression of apelin and apj s increased rapidly at mealtime and significantly decreased after 30 min of feeding. In addition, there were obvious circadian rhythms (higher at nighttime and lower at daytime) in the expression of apelin and apja and apjb . Furthermore, fasting elevated the mRNA levels of apelin and apj s in the hypothalamus, foregut and hepatopancreas. When the 8-day fasted fish were refed, apelin and apj s mRNA expression significantly decreased. However, there were differences in the response to fasting and refeeding between apja and apjb . The roles of apelin and its receptor apjs in fish are poorly understood. Herein, we employed molecular and physiological studies to characterize their structures, distribution and mRNA expression responses to periprandial changes and fasting–refeeding in Cyprinus carpio L. for the first time.
研究领域:
分子生物学
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生理学
·
水产养殖
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
Water
·
2024
·Vol. 16
·期 9
·1295
·
IF 3
Hualong He,Sulin Cai,Siyuan Chen,Qiang Li,Yunchao Luo,Xiaoyi Zeng,Rumeng Ye,Pengwei Wan,Xingjun Tian
This study investigates the combined effect of microplastics and cadmium on the decomposition of litter, the structure of fungal communities, and the feeding behavior of invertebrates in an aquatic ecosystem. Through a series of microcosm experiments, we demonstrate that exposure to MPs and Cd significantly reduced the decomposition of leaf litter. Notably, the cumulative impact of combined MP and Cd exposure was found to be greater than their individual effects. During this process, the carbon–nitrogen ratio of the litter increased, while dehydrogenase activity and fungal biomass were inhibited. Additionally, the relative abundance of Ascomycota and Basidiomycota fungi decreased, weakening their role in the decomposition of leaf litter. Conversely, MPs and Cd reduced the relative content of leaf litter lignin, improving its quality as food, thereby leading to an increase in the feeding rate of invertebrates. This dual effect indicates that micropollutants suppress the decomposition of litter by regulating microbial metabolic activity and fungal community structure but promote invertebrate feeding. Our findings provide crucial insights into the adverse effects of MPs and Cd on the structure and diversity of aquatic fungal communities, which could have long-term impacts on the food webs and nutrient cycling progress of aquatic ecosystems.
研究领域:
水生生态学
·
环境科学
·
微生物学
·
生态毒理学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Zhejiang University-SCIENCE B
·
2024
·
IF 4.7
Jia Xiaodan,Lu Ke,Liang Xufang
Neuropeptide Y receptor Y8 (NPY8R) is a fish-specific receptor with two subtypes, NPY8AR and NPY8BR. Changes in expression levels during physiological processes or in vivo regulation after ventricular injection suggest that NPY8BR plays an important role in feeding regulation; this has been found in only a few fish, at present. In order to better understand the physiological function of npy8br , especially in digestion, we used clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology to generate npy8br −/− Japanese medaka ( Oryzias latipes ). We found that the deletion of npy8br in medaka larvae affected their feeding and digestion ability, ultimately affecting their growth. Specifically, npy8br deficiency in medaka larvae resulted in decreased feed intake and decreased expression levels of orexigenic genes ( npy and agrp ). npy8br −/− medaka larvae fed for 10 d (10th day of feeding) still had incompletely digested brine shrimp ( Artemia nauplii ) in the digestive tract 8 h after feeding, the messenger RNA (mRNA) expression levels of digestion-related genes ( amy, lpl, ctra , and ctrb ) were significantly decreased, and the activity of amylase, trypsin, and lipase also significantly decreased. The deletion of npy8br in medaka larvae inhibited the growth and significantly decreased the expression of growth-related genes ( gh and igf1 ). Hematoxylin and eosin (H&E) sections of intestinal tissue showed that npy8br −/− medaka larvae had damaged intestine, thinned intestinal wall, and shortened intestinal villi. So far, this is the first npy8br gene knockout model established in fish and the first demonstration that npy8br plays an important role in digestion.
研究领域:
分子生物学
·
遗传学
·
水产养殖
·
生理学
✓ 已验证
DOI
PubMed
高影响因子
eLife
·
2025
·
IF 0
Gaohang Wang,Wei Qi,Rui Huang,Liming Wang
Sweet taste perception, a critical aspect of the initiation of feeding behavior, is primarily regulated by an animal’s internal metabolic state. However, non-metabolic factors, such as motivational and emotional states, can also influence peripheral sensory processing and hence feeding behavior. While mating experience is known to induce motivational and emotional changes, its broader impact on other innate behaviors, such as feeding, remains largely uncharacterized. In this study, we demonstrated that the mating failure of male fruit flies suppressed sweet taste perception via dopamine signaling in specific neural circuitry. Upon repetitive failure in courtship, male flies exhibited a sustained yet reversible decline of sweet taste perception, as measured by the proboscis extension reflex (PER) towards sweet tastants as well as the neuronal activity of sweet-sensing Gr5a+ neurons in the proboscis. Mechanistically, we identified a small group of dopaminergic neurons projecting to the subesophageal zone (SEZ) and innervating with Gr5a+ neurons as the key modulator. Repetitive sexual failure decreased the activity of these dopaminergic neurons and in turn, suppressed Gr5a+ neurons via Dop1R1 and Dop2R receptors. Our findings revealed a critical role for dopaminergic signaling in integrating reproductive experience with appetitive sensory processing, providing new insights into the complex interactions between different innate behaviors and the role of brain’s reward systems in regulating internal motivational and emotional states.
研究领域:
神经科学
·
行为生物学
·
感觉生物学
·
分子神经科学
·
神经行为学
✓ 已验证
产品关联
DOI
PubMed
AQUATIC TOXICOLOGY
·
2022
·
IF 5.202
Dong Wang,Guangxin Yang,Shaoguo Ru,Zhenzhong Zhang,Yuejiao Li,Jun Wang
Prometryn, a widely used triazine herbicide in agriculture and aquaculture, has been commonly detected in marine environments, but its effects on the marine copepod are unknown. In this study, marine copepod Tigriopus japonicus was chronically exposed to environmentally relevant concentrations of prometryn to investigate its impacts and potential mechanism of action. The results showed that 0.5, 5, and 50 μg/L prometryn delayed the first spawning time and hatching time, reduced the fecundity, and inhibited the population growth rate. Moreover, exposure to 0.5, 5 and 50 μg/L prometryn decreased food ingestion, the content of C and N elements, nutrient accumulation and body size, but increased the content of 20-hydroxyecdysone (20E). Transcriptome analysis showed that 50 μg/L prometryn down-regulated 1431 genes, which were mainly enriched in lysosome pathway and chitin binding and cuticle construction process. The results of qRT-PCR showed that the expression of key genes involved in juvenile hormone synthesis and chitin metabolic pathways were also inhibited after prometryn exposure. Molecular docking revealed that prometryn could bind to ecdysone receptor (EcR) and UDP-N-acetylglucosamine pyrophosphorylase (UAP), components of the ecdysteroid nuclear receptor complex. Therefore, environmental relevant prometryn delayed the molting and development of T. japonicus by disrupting the ecdysone signal pathway and chitin metabolic pathway through binding to EcR and UAP. This study provides new insights into toxic effects and molecular mechanisms of prometryn on marine copepods.
研究领域:
海洋生态学
·
环境毒理学
·
分子生物学
·
水生毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）