FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Congli Cui,Mingju Li,Xiaoyang He,Na Ji,Yang Qin,Lei Dai,Liu Xiong,Qingjie Sun
This research prepared functional-resistant starch capsules by constructing multiple calcium alginate shells around calcium alginate/starch beads. Scanning electron microscopy revealed a dense structure for each layer of the calcium alginate shell, tightly encapsulating the calcium alginate/starch beads. The rapidly digestive starch content of three-layer calcium alginate shelled starch capsules decreased to 0.50 ± 1.14%, while the resistant starch content increased to 78.25 ± 1.55%. The estimated glycemic index (eGI) experiment suggested that the two-layer shell capsules (eGI: 59.91) and Three-layer shell capsules (eGI: 47.75) were classified as low-glycemic index foods . Furthermore, calcium alginate/corn starch multilayer capsules showed reduced microbial gas production and increased concentrations of propionic acid , isobutyric acid, and valeric acid compared to lactulose in vitro fecal fermentation experiment. Moreover, three-layer capsules and beads promoted a relative abundance of Bacteroidetes but inhibited the reproduction of Proteobacteria and Actinobacteria compared with lactulose and corn starch. These results suggest that constructing multi-layered sodium alginate shells for starch beads can effectively increase the digestive resistance of starch within the capsules and control its fermentation in the colon. This study will have a profound impact on the development of RS1-type resistant starch and be beneficial in the design and production of functional foods for prevention and assistance in the treatment of chronic diseases such as diabetes and obesity.
研究领域:
食品科学
·
营养学
·
微生物学
·
生物技术
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产品关联
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2022
·
IF 9.988
Andong Wang,Dongsheng Zou,Zhihong Xu,Bin Chen,Xiaopeng Zhang,Falin Chen,Manyun Zhang
Repeated and high-dose carbendazim applications have caused serious soil carbendazim contamination, and eco-friendly and economical approaches have been suggested to promote carbendazim removal in agricultural soil . Spent mushroom substrate (SMS) is a special recycled resource after harvesting mushrooms and can be utilized in contaminated soil amendment. The SMS application into agricultural soil might increase antibiotic resistance gene abundances, and the health risks of SMS application might be reduced with reasonable management to adjust the related electron transport of soil nitrification or denitrification. In this study, the SMS and nitrification inhibitor dicyandiamide were used to remediate agricultural soil contaminated with the carbendazim, and the carbendazim contents, soil microbial biomass, activities and community and human disease genes were determined. Compared to the control treatment, the combined applications of SMS and dicyandiamide significantly decreased soil carbendazim content by 38.14% but significantly enhanced soil β -glucosidase, chitinase, arylsulfatase, urease and electron transfer system activities. The relative abundances of Proteobacteria and Actinobacteria were increased by 11.0% and 8.2% with the SMS application, respectively. The carbendazim residues were negatively correlated with the soil pH, electron transfer system activities and relative abundances of Proteobacteria and Actinobacteria. The relative abundances of human disease genes were also dramatically increased with the SMS application, but compared to the SMS alone, extra dicyandiamide application significantly reduced the relative abundances of human disease genes in soils. The SMS applications into fungicide-contaminated soils could generate double-edged sword effects of facilitating fungicide dissipation but leading to potential health risk increase, while applying the dicyandiamide with SMS might be an effective strategy to decrease the negative effect of health risk.
研究领域:
环境科学
·
土壤学
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微生物学
·
生态毒理学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Linsen Ruan,Kaiyu Ye,Zhicheng Wang,Anqi Xiong,Rong Qiao,Jibin Zhang,Zhiyong Huang,Minmin Cai,Chan Yu
As agents in an emerging technology, Hermetia illucens (Linnaeus, 1758) (Diptera: Stratiomyidae) larvae, black soldier fly, have shown exciting potential for degrading antibiotics in organic solid waste, a process for which gut microorganisms play an important role. This study investigated the characteristics of larval gut bacterial communities effected by typical antibiotics. Initially, antibiotics significantly reduced the diversity of gut bacterial species. After 8 days, diversity recovered to similar to that of the control group in the chlortetracycline, tylosin, and sulfamethoxazole groups. Proteobacteria, Firmicutes, and Actinobacteriota were the dominant phyla at the initial BSFL gut. However, after 4 days treatment, the proportion of Actinobacteriota significantly decreased, but Bacteroidota notably increased. During the conversion process, 18, 18, 17, 21, and 19 core genera were present in the chlortetracycline, sulfamethoxazole, tylosin, norfloxacin, and gentamicin groups, respectively. Pseudomonas , Actinomyces, Morganella , Providencia and Klebsiella might be the important genera with extraordinary resistance and degradation to antibiotics. Statistical analyses of COGs showed that antibiotics changed the microbial community functions of BSFL gut. Compared with the control group, (i) the chlortetracycline, sulfamethoxazole, and tylosin groups showed significant increase in the classification functions of transcription, RNA processing and modification，and so on, (ii) the norfloxacin and gentamicin groups showed significant increase in defense mechanisms and other functions. Note that we categorized the response mechanisms of these classification functions to antibiotics into resistance and degradation. This provides a new perspective to deeply understand the joint biodegradation behavior of antibiotics in environments, and serves as an important reference for further development and utilization of microorganisms-assisted larvae for efficient degradation of antibiotics.
研究领域:
环境科学
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微生物学
·
昆虫学
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生物技术
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产品关联
DOI
PubMed
高影响因子
PLANT FOODS FOR HUMAN NUTRITION
·
2016
·Vol. 71
·期 2
·123-128
·
IF 2.276
Ou Juan-ying,Huang Jun-qing,Song Yuan,Yao Sheng-wen,Peng Xi-chun,Wang Ming-fu,Ou Shi-yi
Corn bran is a byproduct produced from corn milling; it is rich in ferulic acid and hemicellulose. In this research, the effects of feruloylated oligosaccharides (FOs) from maize bran on the microbial diversity and profiles in rat feces were investigated through 16S rRNA sequencing. FOs significantly increased bacterial richness and diversity compared with the control and xylooligosaccharides (XOS) alone. In comparison with the control group and the group administrated with XOS, FOs orally administered at 300 mg/kg increased OTU in feces by 57.0 and 24.8 %, and Chao value by 93.4 and 37.6 %, respectively. FOs also influenced obesity- and diabetes-associated bacteria. Oral administration of FOs at 300 mg/kg decreased the ratio of Firmicutes to Bacteroidetes from 477.7:1 to 55.1:1; greatly increased the reads of bacteria that were previously found resistant against diabetes in rats, such as Actinobacteria , Bacteroides , and Lactobacillus ; whereas decreased diabetes-prone bacteria, such as Clostridium and Firmicutes .
研究领域:
微生物学
·
营养学
·
胃肠病学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Yao Li,Ting Gong,Hongjia Lu,Senquan Ma,Xiong Liu
Depolymerized konjac glucomannan (KGM) had better health benefits than natural KGM. Oxidation and enzyme-hydrolysis were two effective ways to prepare depolymerized KGM. Oxidized KGM (OKGM) and enzyme-hydrolyzed KGM (EKGM) varied in molecular structures, verified by nuclear magnetic resonance and atomic force microscope. The differences in chemical structure definitely influenced the fermentability of depolymerized KGM. Herein, effects of OKGM on in vitro fecal fermentation was investigated, comparing with EKGM at approximately same molecular weights. During fermentation, OKGM groups displayed slightly lower reducing sugar concentrations and higher residual carbohydrate contents than EKGM groups. After fermentation, OKGM groups produced more acetic acids and propionic acids than EKGM groups, while EKGM groups generated more butyric acids than OKGM groups. Meanwhile, relative abundances of the Firmicutes and Lactobacillus in OKGM groups were higher, while relative abundances of the Actinobacteria , Fusobacteria and Bacteroides was higher in EKGM groups. OKGM and EKGM differed in modulating gut microbiota.
研究领域:
食品科学
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微生物学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Zulqarnain Haider Khan,Han Yu,Hui Li,Syed Shabi Ul Hassan Kazmi,Ruixia Han,Muhammad Azeem,Xin Sun,Zia Ur Rehman,Gang Li
Reactive oxygen species (ROS) are critical mediators of soil biogeochemical processes. While the production of ROS with biochar (BC) in the rhizosphere has not been explored. We demonstrate that BC and Fe-modified biochar (FeBC), prepared at 400°C and 600°C, influence ROS generation in paddy soil containing biodegradable (polybutylene succinate: PBS) and conventional (polystyrene) microplastics (MPs). FeBC, particularly FeBC prepared at 600°C, enhance ROS (O 2 •− , H 2 O 2 , • OH) production in paddy soil. The results of rhizosphere-focused pot experiment simulating field-like flooded conditions highlight that FeBC significantly increased O 2 •− , H 2 O 2 , • OH concentrations, especially with PBS (25.63, 27.69, 35.82 µM/kg, respectively) surpassing control by 3.1–3.6-folds. Iron plaque formation, water-soluble phenols, and microbial activity further influenced ROS dynamics and accelerated MP degradation via oxidative fragmentation (SEM surface fissures), dissolved organic carbon release (484 mg/kg), and SUVA 254 decline (4.6 folds). Microbial shifts toward Actinobacteria (37–43 %) and Firmicutes (25–28 %) correlated with ROS dynamics, linking biotic-abiotic degradation pathways. This work reveals the novel role of FeBC in rhizosphere ROS generation and the environmental behavior of MPs in BC-treated agricultural systems.
研究领域:
环境科学
·
土壤生物地球化学
·
纳米技术
·
农业化学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC MICROBIOLOGY
·
2024
·Vol. 24
·期 1
·1-14
·
IF 4
Liu Ting,Wang Yin,Hou Zhuoer,Shi Zhenyu,Wang Rongyun,Shi Yanan,Hua Lijiangshan,Wu Lingyun,Xu Min,Ding Xinghong,Sun Qiuhua
Background The guts of mammals are home to trillions of microbes, forming a complex and dynamic ecosystem. Gut microbiota is an important biological barrier for maintaining immune homeostasis. Recently, the use of antibiotics to clear gut microbiota has gained popularity as a low cost and easy-to-use alternative to germ-free animals. However, the effect of the duration of the antibiotic cocktail on the gut microbiome is unclear, and more importantly, the effect of dramatic changes in the gut microbiota on intestinal tissue morphology and local immune response is rarely reported.Results We observed a significant reduction in fecal microbiota species and abundance after 1 week of exposure to an antibiotic cocktail, gavage twice daily by intragastric administration. In terms of composition, Bacteroidetes and Firmicutes were replaced by Proteobacteria. Extending antibiotic exposure to 2–3 weeks did not significantly improve the overall efficiency of microbiotal consumption. No significant histomorphological changes were observed in the first 2 weeks of antibiotic cocktail exposure, but the expression of inflammatory mediators in intestinal tissue was increased after 3 weeks of antibiotic cocktail exposure. Mendelian randomization analysis showed that Actinobacteria had a significant causal association with the increase of IL-1β (OR = 1.65, 95% CI = 1.23 to 2.21, P = 0.007) and TNF-α (OR = 1.81, 95% CI = 1.26 to 2.61, P = 0.001).Conclusion sOur data suggest that treatment with an antibiotic cocktail lasting 1 week is sufficient to induce a significant reduction in gut microbes. 3 weeks of antibiotic exposure can lead to the colonization of persistant microbiota and cause changes in intestinal tissue and local immune responses,
研究领域:
微生物学
·
免疫学
·
胃肠病学
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产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2022
·
IF 13.4
Chen Cai,Xiangfeng Huang,Xiaohu Dai
Centralized sludge treatment plants (CSTPs) are implicated as strong hotspots of antibiotic resistance genes (ARGs). However, the knowledge gap on the fate of intracellular and extracellular ARGs (iARGs and eARGs), and the functionality of resistant hosts limit risk assessment and management of CSTP resistome. Here, the flow of iARGs and eARGs across treatment units and analyses of ARG hosts were systematically explored in three full-scale CSTPs using quantitative metagenomic approaches. We found that 29% of sludge ARGs could be removed, with iARGs being dominant in the produced biosolids. The treatment process significantly affected the variations of iARG and eARG abundance while no significant difference in composition between iARGs and eARGs was observed in CSTPs. 15% of 295 recovered genomes were identified as antibiotic-resistant hosts, among which Actinobacteriota tended to encode multiple resistance. The key functions of ARG hosts were relative to the biological organic removal (e.g., carbohydrates). There also existed relationships between certain resistance mechanisms and functional traits, indicating that ARGs might take part in the physiological process of microorganisms in the sludge treatment. These findings provide important insight into the differential resistome variations and host functionality, which would be crucial in the management of antibiotic resistance in CSTPs.
研究领域:
环境微生物学
·
废水处理
·
抗菌素耐药性
·
宏基因组学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Xiaoying Lin,Jiating Zhao,Wei Zhang,Lina He,Liming Wang,Hong Li,Quancheng Liu,Liwei Cui,Yuxi Gao,Chunying Chen,Bai Li,Yu-Feng Li
Mercury (Hg) is one of the chemicals that bring serious adverse effects to the environment and human beings. Methylmercury (MeHg) is a neurotoxin while inorganic Hg (IHg) is not. Early screening of the neurotoxicity of chemicals may help reduce the occurrence of neurological disorders by minimizing chemical exposure. This work proposed the combined application of gut microbiome, metabolomics and metallomics to differentiate the neurotoxicity between MeHg and IHg in rats. It was found that MeHg caused down-regulated Bacteroides , Firmicutes and Proteobacteria , and up-regulated Actinobacteria and Verrucomicrobia at phylum level, while MeHg caused up-regulated Verrucomicrobiaceae , Desulfovibrionaceae , Helicobacteraceae , Lachnospiraceae and down-regulated Rikenellaceae , Erysipelotrichaceae , Sutterellaceae , Anaeroplasmataceae and Coriobacteriaceae in feces than IHg did at family level; Besides, MeHg brought metabolites change in activation of gut-brain axis pathway than IHg did, such as Glutamate, γ-aminobutyric acid (GABA), Dopamine (DA) and Tryptophan. These differences between MeHg and IHg were further confirmed by the distribution of Hg in the intestine, as well as the level of brain-derived neurotrophic factor (BDNF) in the intestine, brain and serum. Therefore, the difference of toxicity between MeHg and IHg can be well distinguished through feces after exposure for only 24 h, which may be used for the screening of neurotoxicity of other chemicals.
研究领域:
毒理学
·
神经科学
·
微生物学
·
代谢组学
·
金属组学
·
环境科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yue Hu,Lu Zhang,Qing-hui Wen,Xin-peng Cheng,Li-qiang Zhou,Ming-shun Chen,Dai-wei Ke,Zong-cai Tu
This work was to investigate the effect of four prebiotic saccharides gum arabic (GA), fructooligosaccharide (FOS), konjac glucomannan (KGM), and inulin (INU) incorporation on the encapsulation efficiency (EE), physicochemical stability, and in vitro digestion of urolithin A-loaded liposomes (UroA-LPs). The regulation of liposomes on gut microbiota was also investigated by in vitro colonic fermentation. Results indicated that liposomes coated with GA showed the best EE, bioaccessibility, storage and thermal stability, the bioaccessibility was 1.67 times of that of UroA-LPs. The UroA-LPs coated with FOS showed the best freeze-thaw stability and transformation. Meanwhile, saccharides addition remarkably improved the relative abundance of Bacteroidota, reduced the abundances of Proteobacteria and Actinobacteria. The UroA-LPs coated with FOS, INU, and GA exhibited the highest beneficial bacteria abundance of Parabacteroides , Monoglobus , and Phascolarctobacterium , respectively. FOS could also decrease the abundance of harmful bacteria Collinsella and Enterococcus , and increase the levels of acetic acid, butyric acid and iso-butyric acid. Consequently, prebiotic saccharides can improve the EE, physicochemical stability, gut microbiota regulation of UroA-LPs, and promote the bioaccessibility of UroA, but the efficiency varied based on saccharides types, which can lay a foundation for the application of UroA in foods industry and for the enhancement of its bio-activities.
研究领域:
食品科学
·
营养学
·
微生物学
·
药学
✓ 已验证
DOI
PubMed
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2023
·
IF 3.1
Yibo Guo,Ke Shen,Xinshuai Zhang,Hua Huang
l -threonate is the metabolite of vitamin C, while d -erythronate is the metabolite of N -acetyl- d -glucosamine, the nutritional supplement for joint health. They are widely distributed in the environment and human biofluids. Nevertheless, the catabolisms of l -threonate and d -erythronate are sparsely reported. Here we explored the functional diversity of an acid sugar kinase family (Pfam families PF07005-PF17042), and discovered a novel 2-oxo-tetronate kinase. The conserved genome neighborhood of the 2-oxo-tetronate kinase encodes members of class-II fructose-bisphosphate aldolase family (F_bP_aldolase, PF01116) and a dehydrogenase family (PF03446-PF14833). Instructed by this analysis, we experimentally verified that these enzymes are capable of degrading l -threonate into dihydroxyacetone phosphate (DHAP) in Arthrobacter sp. ZBG10, Clostridium scindens ATCC 35704, and Pseudonocardia dioxanivorans ATCC 55486. Meanwhile, a convergent catabolic pathway for d -erythronate was characterized in P . dioxanivorans ATCC 55486. Moreover, the phylogenetic distribution analysis indicates that the biological range of the identified l -threonate and d -erythronate catabolic pathways appears to extend mostly to members of the Actinomycetota , Cyanobacteriota , Bacillota , Pseudomonadota , and Bacteroidota phyla.
研究领域:
微生物学
·
生物化学
·
分子生物学
·
基因组学
·
代谢
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Xinyue Zhang,Tao Lin,Fuchun Jiang,Xue Zhang,Shiyu Wang,Shisheng Zhang
Pipe material and residual chlorine are key factors for the drinking water distribution system, and understanding the biofilm ecosystem is vital for water quality safeguard. The aim of our study was to determine the influence of pipe materials (ductile iron, steel, polyethylene) and chlorination on the biofilm structure and microbial community , as shown by the physicochemical properties , extracellular polymeric substances (EPS) structural characteristics, bacterial community composition, and functional traits. EPS spatial properties were studied based on a semi-quantitative confocal laser scanning microscope (CLSM) description. Regarding the impact of chlorination, residule chlorine (1.0 ± 0.3 mg L −1 free chlorine) could inhibit the bacteria colonization, and initiate a potential response to external disinfectants revealed by the EPS spatial distribution changes and communities variation compared to unchlorinated system. Regarding the impact of pipe material, polyethylene (PE) biofilms displayed lower biomass, loose zoogloea structure, lower proteins and polysaccharides content, and poor microbial diversity in contrast to ductile iron and steel biofilms. Pipe material was the more possible driving factor of the biofilm community composition compared to the chlorination based on principal coordinates analysis (PCoA) and permutational multivariate analysis of variance (PERMANOVA). Actinobacteria was dominant in the PE biofilms (45.57%–83.32%), while Alphaproteobacteria (34.30%–73.22%) and Gammaproteobacteria (6.46%–36.82%) were the major classes in the steel and ductile iron biofilms. The genus Rhodococcus was predominant in the PE biofilms. Rhodococcus , Pseudomonas , and Sphingomonas seemed to have a better growth advantage in the chlorinated system and display a stronger disinfectant resistance. Functional sketch prediction indicated the potential impact of pipe material and chlorination on functional pathway abundnce, possible functional pathways associated with infectious disease included. This study provides insights into the impact of pipe material and chlorination on biofilm structure and microbial community and might help to develop monitoring or maintenance strategies to protect the biosafety of the drinking water.
研究领域:
环境科学
·
微生物学
·
水利工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）