TALANTA
·
2025
·
IF 6.1
Xin Liu,Shiyue Niu,Bin Yang,Jia Liu,Liqian Niu,Xian Wang,Daqian Song,Shuyun Bi
Ultrasensitive Surface-enhanced Raman spectroscopy (SERS) method for the detection of diquat/dipterex was established using bovine serum albumin (BSA)-protected silver nanoparticles (AgNPs) modified MIL-53(Al) (named as BSA/MIL-53(Al)/AgNPs). Compared with unmodified AgNPs, BSA/MIL-53(Al)/AgNPs significantly enhanced the Raman signals of diquat and dipterex and the enhancement factors (EFs) were 1.58 × 10 7 and 2.34 × 10 7 , respectively. The TEM, XRD, TGA, XPS, UV–vis and FT-IR were utilized to characterize BSA/MIL-53(Al)/AgNPs and the binding of the substrate with diquat/dipterex. The optimal measurement conditions were investigated in detail by single factor experiment and response surface model. The impacts of common pesticides and coexisting substances on the determination of diquat/dipterex were studied. Under optimum conditions, linear calibration curves for detecting diquat/dipterex were established with a limit of detection (LOD) of 0.17/0.89 pmol L −1 (3 S 0 / S ). The SERS approaches were used to detect diquat and dipterex in several fruits and vegetables. The recovery was 97.10 %–104.82 % with the relative standard deviation (RSD) of 1.04 %–4.15 % ( n = 5).
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Journal of Integrative Agriculture
·
2023
·
IF 4.8
Jia-nan SUN,Gao-yue SI,Hong-yi LIU,Ya-qian LI,Xin-hua WANG,Jie CHEN
Excessive use of organophosphate pesticides (OP), such as dichlorvos, in farming system poses a threat to human health through potential contamination of environment. To date, biodegradation has been prospected most promising approach to eliminate environmental OP residues. Trichoderma species as a biological control microorganism is often exposed to the chemical pesticides applied in environments, so it is necessary to understand the mechanism of degradation of dichlorvos by Trichoderma . In this study, dichlorvos significantly inhibited the growth, sporulation and pigmentation of T. atroviride T23, and the dichlorvos degradation activity of T23 required the initial induction effect of dichlorvos and the culture conditions, including the nutrient and pH values of the medium. Various changed primary and secondary metabolites released from T23 in the presence of dichlorvos were speculated as the energy and antioxidants for the strain itself to tolerate dichlorvos stress. The results showed that T23 could produce a series of enzymes, especially the intracellular enzymes, to degrade dichlorvos. The activities of the intracellular enzyme generated by T23 were differentially changed along time course and especially relied on initial dichlorvos concentration, ammonium sulfate and phosphate added in the medium. In conclusion, some dichlorvos-induced chemical degradation related enzymes of T23 were proved to be involved in the degradation of dichlorvos.
研究领域:
环境科学
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微生物学
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生物技术
·
农药科学
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DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 43
·25248-25256
·
IF 3.049
Jianhua Zhang,Bo Wang,Yiru Li,Wenhao Shu,Huaying Hu,Lianqiao Yang
An electrochemical acetylcholinesterase biosensor based on silver nanowire, graphene, TiO2 sol–gel, chitosan and acetylcholinesterase has been fabricated successfully for the detection of organophosphate pesticides. The outstanding electrical properties of silver nanowires and graphene, and moreover the self-assembly of these two nanomaterials make the biosensor highly sensitive. Simultaneously, the immobilization efficiency of the enzyme is greatly improved by the action of the TiO2 fixed matrix. Under optimum conditions, the biosensor exhibited excellent performance for the detection of dichlorvos with a linearity in the range of 0.036 μM to 22.63 μM and the detection limit was found to be 7.4 nM. The biosensor was highly reproducible and stable during detection and storage.
研究领域:
分析化学
·
环境科学
·
纳米技术
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemosensors
·
2025
·Vol. 13
·期 8
·306
·
IF 3.7
Junjie Ren,Xia Li,Zhongxing Wang,Li Yu
Trichlorfon, an organophosphorus pesticide widely used in agriculture and other fields, poses a severe risk to both food safety and human health. We developed a photonic crystal film sensing platform for detecting trichlorfon, a hazardous organophosphorus pesticide. The method exploits trichlorfon’s inhibition of acetylcholinesterase (AChE). Normally, AChE catalyzes acetylcholine hydrolysis to produce acetic acid, which decomposes CaCO3to release Ca2+. This triggers calcium alginate hydrogel formation, increasing solution viscosity and trapping water. When trichlorfon inhibits AChE, hydrogel formation fails, leaving the solution in a low-viscosity sol state with abundant free water. Immersing the film in trichlorfon-containing sodium alginate solutions causes water absorption and film swelling due to free water. Higher trichlorfon concentrations reduce hydrogel formation, increase free water, and amplify film swelling, resulting in proportionally higher reflectivity. The platform demonstrates a wide linear range (1–250 ng/mL) and a low detection limit (0.4 ng/mL) for trichlorfon. Successful analysis of real samples confirms its practicality for residue detection. This label-free thin-film sensor shows significant potential for monitoring trichlorfon and other organophosphorus pesticides.
研究领域:
环境监测
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分析化学
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生物传感器
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食品安全
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Wanqi Jiang,Zan Yang,Fei Tong,Siyu Zhang,Lu Zhu,Lei Wang,Lunjing Huang,Kang Liu,Mingming Zheng,Yibin Zhou,Ruyan Hou,Yingnan Liu
In this study, based on the oxidase activity and photothermal effect of manganese dioxide nanosheets (MnO 2 NSs), with thiamine (TH) as the fluorescence response signal and tris (2,2'-bipyridyl) ruthenium (II) hexahydrate as the reference signal, an enzyme-regulated ratiometric fluorescence and photothermal dual-mode probe was constructed for the quantitative detection of organophosphorus pesticide (OPs) residues. OPs reduced the production of the reductive product thiocholine by inhibiting the activity of acetylcholinesterase , thereby regulating the residual amount of MnO 2 NSs. With the increase of OPs concentration, the color of the probe solution gradually transitioned from red to blue, and the temperature gradually increased. Using dichlorvos and chlorpyrifos as pesticide models, the developed probes exhibited sensitive responses to OPs in a wide linear range of 0.1–8000 ng/mL. The detection limits of dichlorvos and chlorpyrifos in fluorescence mode were 1.13 × 10 −3 ng/mL and 0.86 ng/mL, respectively. The corresponding detection limits in photothermal mode were 1.01 ng/mL and 1.02 ng/mL, respectively. The proposed probe displayed excellent anti-interference and reliability in the analysis of OPs residues in real samples. The dual-mode probe with self-verification function is expected to provide more accurate and robust detection results than the single-mode probe, and has a wider application prospect.
研究领域:
分析化学
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环境科学
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材料科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yisong Zhao,Xiaolu Li,Jianmin Chen,Xiong Lu,Yunxia Yang,Dandan Song,Faming Gao
Efficient and sensitive electrochemical biosensors are of great significance for on-site measurement and evaluation of pesticides in the environment, and various functional nanomaterials have been designed and developed to achieve this goal. Herein, a kind of three-dimensional holey hierarchical cobalt-based bimetallic oxides randomly assembled by micro-mesoporous ultrathin nanosheets has been exploited. This unique porous hierarchical structure and bimetal composition not only possesses excellent electron pathways and consequent an enhanced electro-conductivity and better catalytic activity , but also exhibits high electroactive surface area and more vacant sites for enzyme immobilization . Along with the highly conductive and biocompatible layered graphdiyne , the 3D porous Co-based bimetallic oxides have been applied to construct an electrochemical AChE biosensor for monitoring dipterex and omethoate (as model OPs pesticides). As a result, the reported biosensor demonstrates superior sensing performance, for example, the wide detection range of 3.88 × 10 −12 –3.88 × 10 −6 M for diptetex and 4.69 × 10 −13 –4.69 × 10 −7 M for omethoate , together with the relative low detection limit of 0.36 pM and 0.033 pM (S/N = 3), respectively. Additionally, the biosensor is feasible in real samples, and the recoveries (94.8–105.1%) are satisfactory. And a possible mechanism has also been proposed. This study offers a new strategy to modulate the property of metal oxides , which make them more attractive in electrochemical sensors .
研究领域:
材料科学
·
电化学
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环境科学
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纳米技术
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生物传感器
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 16
·1911-1918
·
IF 2.896
Hui-Ling Wu,Bing-Zhi Wang,Yu Wang,Zhi-Li Xiao,Lin Luo,Zi-Jian Chen,Yu-Dong Shen,Zhen-Lin Xu
Diazinon (DAZ) is an organophosphorus pesticide (OP), which is commonly used to prevent and control harmful pests that endanger agricultural products. In this study, we developed a novel heterology coating strategy for the immunoassay of DAZ. The DAZ coating hapten can be directly conjugated to the carrier protein without requiring a spacer arm. This proposed hapten coating strategy is time-saving and significantly improves the sensitivity of the immunoassay due to the lack of a spacer arm. The as-synthesized coating antigen was used to screen the monoclonal antibody (mAb). Finally, the developed indirect competitive enzyme-linked immunoassay (icELISA) showed IC50 and limit of detection (LOD) values of 0.58 ng mL−1 and 8 pg mL−1, respectively. This method exhibited negligible cross-reactivity towards other analogues, and the recoveries of samples (cucumber, cabbage, and lettuce) ranged from 92.6% to 125.4%, with coefficients of variance (CV) below 12%. Good correlation between icELISA and high-performance liquid chromatography mass spectrometry (HPLC-MS/MS) was obtained. The proposed icELISA was an ideal tool for monitoring DAZ residues in food samples.
研究领域:
农业化学
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分析化学
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食品安全
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 9
·1341-1346
·
IF 2.7
Shuhua Zhao,Lei Shi,Xiufeng Zhang,Xiaoran Sun,Wenxuan Zhu,Lijia Yu
The herbicide glyphosate, N-(phosphonomethyl)glycine, has been widely used in the past 40 years, and has had many adverse effects on human health. Here, we constructed a convenient “on–off–on” fluorescent platform for detection of glyphosate via Cu2+ modulated squaraine dye fluorescence quenching. The squaraine dye F-0 exhibited strong fluorescence, which could be quenched by the addition of Cu2+. However, the addition of glyphosate restored the fluorescence intensity of F-0 due to the formation of a Cu2+-glyphosate complex. F-0 was utilized as a fluorescent probe for the quantitative detection of glyphosate, with the lowest detection limit of 13.16 nmol L−1. Furthermore, this method demonstrated high selectivity and anti-interference capabilities. The successful monitoring of glyphosate in real samples was achieved using this detection strategy.
研究领域:
分析化学
·
环境科学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xueqing Zeng,Hengye Chen,Fangyu Long,Xiangmin She,Wei Lan,Wanjun Long,Shuo Wang,Luyu Wei,Yuanbin She,Haiyan Fu
Sulfur-containing pesticides are widely used in agricultural products because of their high insecticidal and herbicidal properties, but the pesticide residues caused by them pose a serious threat to public safety. In this study, a nanometer peroxidase-like enzyme was prepared as a colorimetric probe, constructing a highly sensitive visual colorimetric sensor for detection of 4 sulfur-containing pesticides (thiophanate-methyl: TM; cartap: CT; dimethoate: DT; temephos: TP). Detailedly，the outer electrons of Au-Ag NCs can catalyze the hydrogen peroxide to oxidize TMB to blue, while the positive sulfur-containing pesticides can interact electrostatic with the negative charge of Au-Ag NCs to expose the silver element in the inner layer. Moreover, sulfur in pesticides binds to silver atoms, resulting in the looser structure and disintegration of Au-Ag NCs, ultimately blocking the pseudo-peroxidase activity to produce a distinct color signal. The results demonstrated that all four pesticides exhibited a good linearity (R 2 > 0.99) over a wide concentration range with a low detection limit of 0.03 mg/L. In real samples such as green tea, cabbage, goji berry, apple etc., the recovery rate for added standards of visual sensor based on smartphone APP ranged between 90 % and 115 %, which is significantly better than that obtained by UV-Vis spectrum, while RSD remained below 5 %. Compared with the recovery results of LC-MS, the deviation between these two methods is less than 10 %. The visual sensor based on Au-Ag NCs nano peroxidase-like enzyme offers a novel approach for highly specific and sensitive detection of sulfur-containing pesticides in food safety monitoring.
研究领域:
分析化学
·
食品安全
·
生物传感
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ziyi Qin,Jinju Xu,Yuanxin Cao,Chunhui Liao,Tian Lan,Shuyun Shi
Highly sensitive monitoring of pesticides has garnered attention for food safety. Here, a ratiometric fluorescent probe (AG-CDs-Al 3+ /CdTe QDs) was fabricated for specific and sensitive sensing of glyphosate. Surface modifications revealed the chelation-enhanced fluorescence process between the surface -OH of AG-CDs and Al 3+ . Positively charged green-emission AG-CDs-Al 3+ assembled with negatively charged red-emission CdTe QDs, and photoinduced electron transfer (PET) occurred from CdTe QDs to AG CDs-Al 3+ . Glyphosate specifically coordinated with Al 3+ , causing remarkable variations in F 500 /F 630 (linear range, 0.2–4.0 μM; detection limit, 5.7 nM) and a distinguishable green-yellow-red color change on the paper platform (linear range, 0.5–8.0 μM; detection limit, 26 nM). Furthermore, for real sample analysis, the sensor has the advantages of fast response (5 min), excellent accuracy (spiked recoveries, 95.0–105.0 %), high precision (relative standard deviation, < 3.33 %), and superior specificity, which endowed the designed sensing platform with great potential practicality in the field of food analysis.
研究领域:
食品安全
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Junjie Qi,Wenhui Hao,Jingkun Li,Yuqi Wan,Guoyong Jiang,Yukihiro Ozaki,Fuwei Pi
Phoxim is a broad-spectrum organophosphorus pesticide widely used in the world; however, its neurotoxicity and potential risk pose a significant threat to human health and ecosystems. To address this issue, based on the same metal clusters and similar topologies, a MOF-on-MOF composite strategy, i.e., PCN222(Fe)@NU-1000, was developed for rapid evaluation of phoxim via ratiometric fluorescence and colorimetric dual-signal output. Herein, the inner core, PCN222(Fe), imparts peroxidase-like catalytic activity enabling colorimetric detection based on the 3,3′,5,5′-tetramethylbenzidine (TMB) and the solution decolourisation caused by acetylcholinesterase (AChE) products, while the outer shell, NU-1000, provides a strong fluorescence emission at 461 nm. This core-shell structure also facilitates fluorescence resonance energy transfer (FRET), whereby phoxim selectively quenches the fluorescence of NU-1000 without affecting PCN222(Fe), enabling ratiometric fluorescence sensing. This integrated dual-signal system-comprising ratiometric fluorescence and enzyme-mimetic colorimetry-ensures signal cross-verification and improved detection accuracy. The fluorescence mode exhibits a quantification range of 5.0 ng/mL to 4.0 μg/mL with a detection limit of 4.0 ng/mL, while the colorimetric mode shows a linear range from 2.5 ng/mL to 25.0 ng/mL and a detection limit of 1.76 ng/mL. Furthermore, the method accurately evaluates phoxim levels in fruits and vegetables such as apples and celery, achieving recoveries ranging from 95 % to 115 % and a relative standard deviation (RSD) of less than 9 %. This MOF-on-MOF dual-signal sensing strategy is designed to be simple, user-friendly, and highly reliable, providing significant value for the development of rapid and sensitive approaches in food and environmental science.
研究领域:
分析化学
·
材料科学
·
环境科学
·
食品科学
·
纳米技术
·
传感器技术
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2017
·Vol. 10
·期 4
·417-421
·
IF 1.9
Jing-yan Kang,Yu-jie Zhang,Xing Li,Chen Dong,Hong-yu Liu,Li-jing Miao,Paul J. Low,Zhi-xian Gao,Narayan S. Hosmane,Ai-guo Wu
A colorimetric sensor for the pesticide pymetrozine (PYM) based on analyte-induced aggregation of melamine-modified gold nanoparticles (M-Au NPs) has been developed with high selectivity and sensitivity with a detection limit of 80 nM by the naked eye and 10 nM by UV-Vis spectroscopy. This colorimetric sensing design exhibits lower detection limits, compared with limits of 46–148 nM for existing instrumental methods. The new aggregation-induced nanoparticle sensor is applicable for rapid colorimetric detection of PYM in real water environments (tap water and lake water) and food samples (green tea and apple juice). The technical approach for the colorimetric sensor is easier and simpler than detection of PYM by, for example, HPLC whilst giving equivalent results within the error of the measurements.
研究领域:
环境科学
·
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）