SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Shang Li,Yi Tao,Guo-Hua Dao,Hong-Ying Hu
The risk of harmful algal blooms (HABs) in the water recharged with reclaimed water is a bottleneck for water reuse . The suppression effects and mechanisms of the combination of UV-C and berberine on Microcystis aeruginosa and Scenedesmus obliquus in reclaimed water were investigated. Mono UV-C irradiation at 75 mJ cm −2 could suppress the growth of M. aeruginosa for 7 d and that at 90 mJ cm −2 could suppress the growth of S. obliquus for 5 d. UV-C irradiation combined with 0.2–2 mg L −1 berberine lengthened the inhibition period of M. aeruginosa to 10- > 22 d and that of S. obliquus to 7- > 22 d and induced more rapid lethal effects on the harmful microalgal cells, in significant synergetic patterns. The combination of UV-C and berberine suppressed total, intracellular and extracellular microcystin-LR (MC-LR) more effectively and decreased the MC-LR quota significantly, which further reduced the risks of microcystin production and release. Furthermore, synergetic mechanisms of the combined treatments were systematically investigated from the aspects of photosynthetic system (photosynthetic activity and pigments), metabolic activity (ATP and membrane potential), oxidation stress (reactive oxygen species (ROS) and glutathione (GSH)), and apoptosis-like cell death (phosphatidylserine (PS) ectropion, caspase-3 activity and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) positive rate). The combination treatment provided a joint attack of UV-C and berberine on photosynthetic transport chain of photosynthetic system II (PS II), and a synergetic pathway to achieve more severe disruptions in energy metabolism as well as aggravated oxidative stress . The accumulated ROS enhanced increases in programmed cell death (PCD) indicators of both microalgal species, which contributed to the enhancement effects on growth suppression. The results showed that the combination treatment achieved lower dose requirements of both UV-C irradiation and berberine for inducing the same inhibition effects on microalgal cells, which was promising to be applied in the HABs control of reclaimed water.
研究领域:
环境科学
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藻类学
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水处理工程
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 12
·4774
·
IF 4.6
Qingqing Xiang,Ying Zhou,Chengxia Tan
Prothioconazole (PTC) is a broad-spectrum triazole fungicide with one asymmetric center and consists of two enantiomers,R-(−)-PTC andS-(+)-PTC. To address the concern of its environmental safety, the enantioselective toxic effects of PTC onScendesmus obliquus(S. obliquus) were investigated. PTC racemates (Rac-PTC) and enantiomers exhibited dose-dependent acute toxicity effects againstS. obliquusat a concentration from 1 to 10 mg·L−1. The 72 h-EC50 value ofRac-,R-(−)-, andS-(+)-PTC is 8.15, 16.53, and 7.85 mg·L−1, respectively. The growth ratios and photosynthetic pigment contents of theR-(−)-PTC treatment groups were higher than theRac- andS-(+)-PTC treatment groups. Both catalase (CAT) activities and esterase activities were inhibited in theRac- andS-(+)-PTC treatment groups at high concentrations of 5 and 10 mg·L−1, and the levels of malondialdehyde (MDA) were elevated, which exceeded the levels in algal cells for theR-(−)-PTC treatment groups. PTC could disrupt the cell morphology ofS. obliquusand induce cell membrane damage, following the order ofS-(+)-PTC ≈Rac-PTC >R-(−)-PTC. The enantioselective toxic effects of PTC onS. obliquusprovide essential information for its ecological risk assessment.Keywords:prothioconazole;toxicity effects;Scenedesmus obliquus;enantioselectivity
研究领域:
环境科学
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毒理学
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生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
HARMFUL ALGAE
·
2018
·
IF 4.138
Miaomiao Zhao,Xinyi Chen,Ning Ma,Qiuyue Zhang,Dong Qu,Ming Li
To form Microcystis blooms, Microcystis must be competitively dominant to other phytoplankton species to produce enough biomass. The aim of this study was to determine the competition mechanisms between Microcystis aeruginosa and Scenedesmus obliquus . M. aeruginosa and S. obliquus were separately cultured in the filtrate of mono and mixed cultures of M. aeruginosa and S. obliquus with varying treatments concerning N, P and iron availability. The inhibition rate for M. aeruginosa was 20–31% when cultured in mono and mixed culture filtrates enriched with N and P, but this rate was reduced to −15 to 19% when cultured in filtrates enriched with N, P and iron. The inhibition rate for M. aeruginosa decreased from 80 to 100% to 11–39% in dialytic filtrates. However, there were no differences in inhibition rate for S. obliquus, regardless of filtrate or dialytic filtrate treatments. The potential allelochemical, 2-butyl-octanol (0.144 mg L −1 ), was found to have little inhibitory effect to M. aeruginosa or S. obliquus . Thus, previously reported allelopathy to Microcystis would be overestimated. We also report a new mechanism in phytoplankton competition in this study. The phytoplankton secreted humic acid-like substances that can reduce the bioavailability of iron, resulting in the inhibition of other phytoplankton.
研究领域:
生态学
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环境科学
✓ 已验证
DOI
PubMed
高影响因子
Plants-Basel
·
2020
·Vol. 9
·期 12
·1748
·
IF 2.762
Zhou Li,Philippe Juneau,Yingli Lian,Wei Zhang,Shanquan Wang,Cheng Wang,Longfei Shu,Qingyun Yan,Zhili He,Kui Xu
The effects of the photocatalytic toxicity of titanium dioxide nanoparticle (nano-TiO2) on phytoplankton are well understood. However, as UV light intensity decreases sharply with the depth of the water column, the effects of nano-TiO2itself on deeper water phytoplankton, such as green algae, need further research. In this research, we investigated the effects of three sizes of TiO2(10, 50 and 200 nm) on the photosynthetic and antioxidative processes ofScenedesmus obliquusin the absence of UV light. We found that 50 nm and 10 nm TiO2(10 mg/L) inhibited growth rates and the maximal photosystem II quantum yield compared to the control inScenedesmus obliquus. The minimal and maximal fluorescence yields, and the contents of reactive oxygen species and lipid peroxidation, increased, indicating that photosynthetic energy/electrons transferred to oxygen and induced oxidative stress in nano-TiO2-treated samples. In addition, we found that aggregations of algae and 10 nm TiO2were present, which could induce cell membrane disruption, and vacuoles were induced to cope with nano-TiO2stress inScenedesmus obliquus. These results enhance our understanding of the effects of nano-TiO2on the photosynthetic and antioxidative processes of green algae, and provide basic information for evaluating the ecotoxicity of nano-TiO2in freshwater ecosystems.Keywords:titanium dioxide nanoparticle;photosynthesis;oxidative stress;Scenedesmus obliquus
研究领域:
环境科学
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水生生态学
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植物生理学
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DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
·
2018
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IF 2.776
Yue Meng,Se Wang,Zhuang Wang,Nan Ye,Hao Fang
The present study investigated the impacts of dissolved organic matters (DOM) on joint toxicity involved in zinc oxide nanoparticles (ZnO NPs) and tetrabromobisphenol A (TBBPA) at relevant low-exposure concentrations (<1 mg/L). It was found that ZnO NPs in single and combined systems exhibited severe inhibition effects on a freshwater microalgae Scenedesmus obliquus . However, the presence of DOM slightly alleviated the growth inhibition toxicity induced by the binary mixtures of ZnO NPs and TBBPA. Ultrastructure analysis revealed that ZnO NPs caused structural damage to cells, including plasmolysis, membrane destruction, and the disruption of thylakoid in the chloroplast, regardless of the presence of coexisting substances. Oxidative stress biomarker quantitative analysis and in situ observations indicated that the massive accumulation of reactive oxygen species in the binary mixtures of ZnO NPs and TBBPA caused severe oxidative damage, but the presence of DOM significantly mitigated the damage.
研究领域:
环境科学
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毒理学
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水生生态学
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产品关联
DOI
PubMed
高影响因子
International Journal of Environmental Research and Public Health
·
2022
·Vol. 19
·期 20
·13415
·
IF 4.614
Tingting Zhang,Mei Shi,Hao Yan,Cheng Li
Heavy metal pollution and water eutrophication are still the main issues to be solved in the environmental field. To find a biological control method for Cd2+-contaminated water or combined eutrophication and Cd2+pollution water, the effects of salicylic acid on heavy metal Cd2+resistance in eukaryotic algaeScenedesmus obliquusandChlorella pyrenoidosaand its mechanisms were studied. The results showed that the inhibition rates of 3.0 mg/L Cd2+stress group at 96 h were 67.0% onS. obliquusand 61.4% onC. pyrenoidosaand their uptake of Cd2+was 0.31 mg/g and 0.35 mg/g, respectively. When adding the different concentrations of salicylic acid while stressed by 3.0 mg/L Cd2+, the hormesis phenomenon of low dose stimulation and high dose inhibition could be seen, and the inhibition rates of 30 mg/L~90 mg/L salicylic acid addition groups were significantly lower than those of the Cd2+stress group alone, which were statistically significant (p< 0.05) and the absorption of Cd2+was dramatically improved. Except for the 120 mg/L salicylic acid addition group, the chlorophyll fluorescence parameters (Fv/Fm and YII), glutathione peroxidase (GSH-Px) and glutathione-S-transferase (GST) activities of all the other concentration groups were significantly higher than those of the Cd2+stress group alone,p< 0.05.; the algal cell morphology in low concentration groups (30 mg/L and 60 mg/L) was also less damaged than those in the Cd2+stress group alone. These indicate that the low concentrations of salicylic acid can counteract or protect the algal cells from Cd2+attack, the mechanisms, on the one hand, might be related to the chelation of heavy metals by salicylic acid, resulting in the decrease of the toxicity of Cd2+; on the other hand, low concentrations of salicylic acid can stimulate the growth of these two algae, improve their photosynthetic efficiency and antioxidant capacity, as well as maintain the relative integrity of algal morphological structure.Keywords:Cd2+pollution;eutrophication;salicylic acid;S. obliquus;C. pyrenoidosa
研究领域:
环境科学
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植物学
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生态毒理学
✓ 已验证
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2019
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IF 4.527
Kun Yan,Yanhua Liu,Qiulian Yang,Weixia Liu,Ruixin Guo,Jinhong Sui,Zhengyu Yan,Jianqiu Chen
Quantum dots (QDs), as a kind of novel nanomaterial , have the extensive applications in various fields, inevitably leading to increasing risks for the ecological environment. The mobilization of cadmium including metal smelting and subsequent machining for multifarious applications has caused the release of cadmium element into the environment. In this study, we evaluated the potential toxicity of a novel nanoparticle material CdSe QDs, using two green algae Chlorella pyrenoidosa and Scenedesmus obliquus . The impact of CdSe QDs and cadmium ions on algae and the sensitivity of the two algae on target compounds were also considered and compared. Our results showed the algal growth rates and chlorophyll content decreased with increasing exposure concentrations and durations. Moreover, the glutathione levels were decreased while the activities of superoxide dismutase increased, exhibiting their pivotal functions in defeating toxic stress. The increment of malondialdehyde levels revealed that the stresses of CdSe QDs and cadmium ions were contributed to the occurrence of oxidative damage. Our study also indicated that the impact of CdSe QDs was stronger than that of cadmium nitrate and the algal response was also species-specific. In addition, the TEM photographs of the algal ultrastructure showed the presence of surface attachment and uptake of QDs.
研究领域:
环境科学
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毒理学
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水生生物学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Yanzhuo Zhang,Rui He,Jing Zhao,Xiaozhuan Zhang,Alexandr V. Bildyukevich
For the first time, biochar was prepared by changing the polystyrene (PS) content in sludge, and the efficiency of antibiotics removal by biochar was evaluated after fermentation aging. Fermentation aging affects the efficiency of antibiotics removal by reducing the specific surface area and active sites of biochar. The antibiotics removal efficiency of different types of biochar after aging decreased by 5.95%–13.59%. Owing to the biotoxicity of biochar, the relative abundance of most communities decreased during fermentation, whereas Anaerolineae still increased (14.29% to 33.05% or 33.02%). However, controlled experiments confirmed that biochar was much less toxic to Scenedesmus obliquus than to antibiotics, with concentrations of 11.09 × 10 5 cells/mL and 0.188 × 10 5 cells/mL, respectively. With the positive effect of environmentally persistent free radicals (EPFRs) considered, increasing the PS content in sludge facilitated the removal of antibiotics by biochar. This study assesses the stability of biochar in removing antibiotics after long-term microbial aging.
研究领域:
环境科学
·
生物技术
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微生物学
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化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2016
·
IF 4.839
Fan Zhang,Zhuang Wang,Se Wang,Hao Fang,Mindong Chen,Defu Xu,Lili Tang,Degao Wang
Understanding how engineered nanoparticles (ENPs) interact with natural organic acids is important to ecological risk assessment of ENPs, but this interaction remains poorly studied. Here, we investigate the dispersion stability, ion release, and toxicity of yttrium oxide nanoparticles (nY 2 O 3 ) suspensions after exposure to two low molecular weight natural organic acids (LOAs), namely benzoic acid and gallic acid. We find that in the presence of LOAs the nY 2 O 3 suspensions become more stable with surface zeta potential more positive or negative, accompanied by small agglomerated size. LOA interaction with nY 2 O 3 is shown to promote the release of dissolved yttrium from the nanoparticles, depending on the concentrations of LOAs. Toxic effects of the nY 2 O 3 suspensions incubated with LOAs on Scenedesmus obliquus as a function of their mixture levels show three types of signs: stimulation, inhibition, and alleviation. The mechanism of the effects of LOAs on the nY 2 O 3 toxicity may be mainly associated with the degree of agglomeration, particle-induced oxidative stress , and dissolved yttrium. Our results stressed the importance of LOA impacts on the fate and toxicity of ENPs in the aquatic environment.
研究领域:
环境科学
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纳米技术
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生态毒理学
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水化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Mostafa Gouda,Kai Chen,Xiaoli Li,Yufei Liu,Yong He
Microalgae single-cell electrochemical characteristics are playing an important key role in its industrial application and functionality. This study conducted a single-cell electrochemical study to explain the influence mechanism of intracellular phenolic and flavonoid contents of microalgae on its surface electric charge. In the method, four microalgae species, Arthrospira platensis (AP), Chlorella vulgaris (CV), Phaeodactylum tricornutum (PT), and Scenedesmus obliquus (SO), were studied through single-cell electrochemical current (SCC), single-cell micro-Raman spectrometry (SCMR), and other physicochemical methods. Also, they were correlated with the chemical analytical methods. In the results, PT and AP phenolics, flavonoids, and antioxidant activities were significantly high compared to CV and SO. On the other hand, SO had the highest SCC compared to AP and PT. Also, SCMR at 1514 cm −1 was negatively correlated with antioxidant activity by nitric oxide (r 2 = -0.80). In conclusion, single-cell current measurement by gold microelectrode can verify the antioxidant activity of the different microalgae species. Besides, this work provides a simple micro-level study of the single-cell phytochemicals to better explain their exact functional impacts compared to the methods of macro-analyses that use millions of cells, which reduces their sensitivity. In which, it can be applied in the real-time functionality evaluation during microalgae cultivation.
研究领域:
微生物学
·
电化学
·
分析化学
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光谱学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
COMPUTERS AND ELECTRONICS IN AGRICULTURE
·
2021
·
IF 5.565
Bingquan Chu,Kai Chen,Xiaoxiao Pan,Qianying Wu,Shiwang Liu,Jinyan Gong,Xiaoli Li
Microalgae lipid and its degree of fatty acid unsaturation (DU) have the ability to undergo programmatic changes in response to different environmental and culture conditions. A real-time and reliable approach to monitor the change patterns of lipid-accumulation and DU is crucial for microalgal culture and bioprocess control. In this study, a fast visual and nondestructive method based on visible/short-wave near-infrared hyperspectral imaging (V/SNIR HSI) at 400–1000 nm region was developed to investigate the dynamic changes and content distribution of lipids and DU of Scenedesmus obliquus treated by nitrogen-low stress (0.3 g/L NaNO 3 , NL) and nitrogen-free stress (0 g/L NaNO 3 , NF). After collecting the average spectra from the region of interests (ROIs), the competitive adaptive reweighted sampling (CARS) algorithm and the successive projection algorithm (SPA) were applied to pick effective wavebands from the spectral data for the establishment of the lipid concentration and DU estimation models including partial least squares regression (PLSR) and multiple linear regression (MLR) . The prediction results show the CARS-PLSR (R p = 0.9762, RMSE p = 0.0143) and CARS-MLR (R p = 0.9477, RMSE p = 0.5718) performed the best for lipids and DU of S. obliquus , respectively. Based on the optimal models, the spatial distribution of lipid abundance and DU in liquid suspension were visualized, which could be helpful in exploring the dynamic process of lipid synthesis and DU of S. obliquus. The overall results show that V/SNIR HSI is a promising technique for fast and noninvasively monitoring the lipid-accumulation and its characteristics i.e. DU in situ, and is expected to be applied for efficient and high-quality lipid production in microalgae industries.
研究领域:
生物技术
·
环境科学
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光谱学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
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2021
·
IF 8.198
Jilin Yuan,Ming Chen,Wenhao Xiang,Chuanbao Xiao,Nianbing Zhong,Qiang Liao
Rapid and continuous degradation of toxic organic pollutants and conversion to microalgae biomass are attracting enormous interest for resource recovery and wastewater treatment. Aiming toward this goal, we develop and demonstrate a novel photocatalytic-biological system in which photocatalytic optical hollow fibers coated with the N-doped TiO2 photocatalyst and a Scenedesmus obliquus biofilm cooperatively convert phenol to microalgae biomass. The photocatalytic optical hollow fibers exhibit high UV–visible photocatalytic activity to rapidly degrade phenol and emit visible light for biofilm growth, lipid synthesis, and O2 production. O2 produced as an electron acceptor is transferred to the surface of the photocatalytic optical hollow fibers, limiting electron–hole recombination and generating reactive oxygen species such as •OH and •O2–. Upregulation of nicotinamide adenine dinucleotide + hydrogen and adenosine triphosphate and enrichment of Rhodococcus and Pseudomonas in the biofilm enhance the resistance to phenol toxicity, maintain rapid and stable biofilm growth, and increase lipid content. This photocatalytic-biological system rapidly and sustainably converts phenol to microalgae biomass.
研究领域:
环境科学
·
生物技术
·
废水处理
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资源回收
·
光催化
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）