JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2026
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IF 5.3
Xiaolun Peng,Ruijie Xu,Liang Zhang,Xingyao Li,Junhui Xu,Dongyun Huang,Yazhen Wang
A sensitive electrochemical sensor was proposed for neohesperidin dihydrochalcone (NHDC) detection using copper oxide/ zinc imidazole framework 8@ carbon nanotubes modified glassy carbon electrode (CuO/ZIF-8@CNTs GCE). The multi-component CuO/ZIF-8@CNTs GCE was prepared by two-step drop-casting method and characterized by Field-emission scanning electron microscopy (FE-SEM). The effective combination of CuO and ZIF-8@CNTs enhanced the electron transfer and catalytic activity of the electrode and greatly strengthened the oxidation current of NHDC, which was about 5.6-fold that of the CuO GCE and 2.3-fold that of the ZIF-8@CNTs GCE. Inspired by this, the CuO/ZIF-8@CNTs GCE was used for NHDC detection and some crucial parameters were optimized. The reaction mechanism at CuO/ZIF-8@CNTs GCE was confirmed to involve two-step two-electron process by cyclic voltammetry (CV) measurements. Finally, the sensor achieved NHDC detection ranging from 0.03 to 10 μM using differential pulse voltammetry (DPV) technique, with a detection limit of 16.0 nM. The satisfactory recoveries of 92.9–107.8 % in liquor samples also demonstrated its practicability in actual samples, matching well with that of high-performance liquid chromatography (HPLC). This work not only designs a simple detection method for NHDC but also opens up a paradigm for leveraging multi-component MOF composite electrode materials for food safety monitoring.
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AMB Express
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2022
·Vol. 12
·期 1
·1-14
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IF 4.126
Yu Bo,Luo Shiyu,Ding Yuhan,Gong Zijie,Nie Ting
αL-rhamnosidase (EC 3.2.1.40) has been widely used in food processing and pharmaceutical preparation. The recombinant α-L-rhamnosidase N12-Rha from Aspergillus niger JMU-TS528 had significantly higher catalytic activity on α-1,6 glycosidic bond than α-1,2 glycosidic bond, and had no activity on α-1,3 glycosidic bond. The activities of hydrolyzed hesperidin and naringin were 7240 U/mL and 945 U/mL, respectively, which are 10.63 times that of native α-L-rhamnosidase. The activity could maintain more than 80% at pH 3–6 and 40–60℃. Quantum chemistry calculations showed that charge difference of the C-O atoms of the α-1,2, α-1,3 and α-1,6 bonds indicated that α-1,6 bond is most easily broken and α-1,3 bond is the most stable. Molecular dynamics simulations revealed that the key residue Trp359 that may affect substrate specificity and the main catalytic sites of N12-Rha are located in the (α/α) 6 -barrel domain.
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RESOURCES CONSERVATION AND RECYCLING
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2022
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IF 13.716
Dan Yu,Fei Liu,Changdong Zou,Xiao Yang
Shortage of soluble Fe in seawater is a limiting condition for benthic seaweeds growth. Utilizing steelmaking slag as the Fe source for seaweeds growth has been proposed. Aiming to find a cost-effective method to promote Fe release, we investigate the effect of adding municipal green wastes. By conducting leaching experiments in freshwater, a sharp improvement in Fe release was observed with the addition of tangerine peel together with steelmaking slag. The flavonoids supplied from the tangerine peel, vicenin-2, apigenin, hesperidin, vitexin, hesperetin and diosmin were observed to have a promotion effect on Fe release. Formation of stable flavonoid-Fe(II) chelates changes the thermodynamic property of Fe(II) in water and disturbs the equilibrium between Fe(II) in slag and Fe(II) in water, which promotes Fe release from slag. These results demonstrate the feasibility to develop a technology for Fe supply in seawater by utilizing the mixture of steelmaking slag and municipal green wastes.
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Foods
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2022
·Vol. 11
·期 13
·1920
·
IF 5.561
Qi Quan,Wei Liu,Jiajing Guo,Meiling Ye,Juhua Zhang
Six lactic acid bacteria strains were used to study the effects on physicochemical characteristics, antioxidant activities and sensory properties of fermented orange juices. All strains exhibited good growth in orange juice. Of these fermentations, some bioactive compositions (e.g., vitamin C, shikimic acid) and aroma-active compounds (e.g., linalool, 3-carene, ethyl 3-hydroxyhexanoate, etc.) significantly increased inLactiplantibacillus plantarumandLactobacillus acidophilussamples. DPPH free radical scavenging rates inL. plantarumandLacticaseibacillus paracaseisamples increased to 80.25% and 77.83%, respectively. Forty-three volatile profiles were identified, including 28 aroma-active compounds. 7 key factors significantly influencing sensory flavors of the juices were revealed, including D-limonene, linalool, ethyl butyrate, ethanol,β-caryophyllene, organic acids and SSC/TA ratio. The orange juice fermented byL. paracasei, with more optimization aroma-active compounds such as D-limonene,β-caryophyllene, terpinolene andβ-myrcene, exhibited more desirable aroma flavors such as orange-like, green, woody and lilac incense, and gained the highest sensory score. Generally,L. paracaseifermentation presented better aroma flavors and overall acceptability, meanwhile enhancing antioxidant activities.Keywords:orange juice;lactic acid fermentation;physicochemical characteristics;antioxidant activities;volatile profiles;sensory properties
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Food & Function
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2022
·Vol. 13
·期 9
·5061-5074
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IF 6.317
Jie Zhao,Lin Huang,Renjie Li,Zhuangwei Zhang,Jin Chen,Hongjin Tang
Gliadin, as a main component of wheat storage protein, is used as a drug encapsulation and delivery system owing to its specific characteristics. Flavonoids are regarded as active natural products with a variety of pharmacological effects. In this study, an integrated method including UV-vis, fluorescence, and FT-IR spectroscopy and molecular modelling was applied to explore the structure–affinity relationship and the interaction nature between a library of flavonoids and gliadin. The characteristic UV-vis spectral changes of gliadin mediated by flavonoids with absorption bands at 218 and 278 nm demonstrated the existence of an interaction depending on generating the ground-state complexes. Fluorescence quenching results showed that the intrinsic fluorescence of gliadin could be effectively quenched by flavonoids coupled with the formation of flavonoid–gliadin complexes through the static quenching mechanism. The structure–affinity relationship revealed the critical structural elements associated with the binding affinity on gliadin and underlined the favorable substituents at the specific positions of flavonoid skeletons leading to a stronger binding potency. From the analysis of synchronous fluorescence spectra, flavonoids could cause the conformation change of gliadin and impact the microenvironment around TYR and TRP residues. Moreover, the ANS fluorescent probe assay suggested that these flavonoids also influenced the surface hydrophobicity of glaidin based on the further exposure or blocking of hydrophobic domains. Molecular modelling was subsequently performed and illustrated the proposed binding conformation of flavonoids on gliadin. Combined with the FT-IR spectra, these results further confirmed the important role of hydrophobic interactions and hydrogen bonds in their binding process.
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Journal of Pharmaceutical Innovation
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2022
·Vol. 17
·期 4
·1305-1313
·
IF 2.538
Lu Shao Hua,Zhang Ming Cai,Zhai Hong Lin,Bi Ke Xin,Zhao Bing Qiang
Purpose Due to the large number of chemical components in Chinese patent medicine, the quality control analysis is faced with greater challenges. Although different detection techniques, analytical conditions, and sample pretreatments are often employed respectively to quantify the multiple target components in complex mixtures, it is difficult to meet the practical requirement of productive enterprises. Methods Tchebichef moments (TMs) were calculated from the raw HPLC–DAD spectra of samples and the linear quantitative models were established by stepwise regression for the eight active components (four alkaloids, one statin, and three glycosides) in Daizongfang (one kind of Chinese patent medicines), respectively. Results The correlation coefficients of prediction ( \({R}_{p}^{2}\) ) were higher than 0.9159, and the spiked recovery rates were from 93.4 to 108.2%. Compared with the N-PLS and MCR-ALS models, the TM models are more accurate and reliable. Conclusions This study not only indicates that chemometrics approach has outstanding advantages for the identification of complex signal, but also provides another way for the rapid analysis of quality control in production.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
·
IF 4.952
Lingrong Wen,Mingyang He,Chunxiao Yin,Yueming Jiang,Donghui Luo,Bao Yang
Sour orange ( Citrus aurantium ) is a popular fruit with health benefits. Phenolics are the dominant bioactive compounds in this fruit. However, the full phenolics composition remains unclear. In this work, UHPLC-MS/MS was used to qualify and quantify the phenolics in sour orange fruits of cv. Morocco (MSO) and United (USO). Fifty-eight phenolics, including 26 flavonoid glycosides , 22 polymethoxylated flavonoids, 8 coumarins and 2 phenolic acids , were identified. Four limonoids were characterized. Flavonoid glycosides were the leading phenolics in MSO, while polymethoxylated flavonoids (including nobiletin, tangeritin and sinensetin) were dominant in USO. The phenolic extracts showed good antioxidant activities . USO extract had a significantly ( p < 0.05) stronger neuroprotective activity than MSO extract. Auraptene, nobiletin and tangeritin were highly responsible for the neuroprotective activity. The results were helpful for the development of functional foods with sour orange.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
·
IF 3.232
Hongjin Tang,Lin Huang,Dongsheng Zhao,Chunyong Sun,Ping Song
The molecular structure properties-binding affinity relationship of a series of flavonoids and bovine serum albumin (BSA) was investigated in vitro from comparing the binding constants determined through the fluorescence method . As a result, the binding process was greatly influenced by different structural elements or substituents of flavonoids under analysis. The hydroxylation at the positions C3, C6, C4’, C5’ (for type I) and C5, C3’ (for type II) were in favor of forming hydrogen bonds with the amino acids of BSA, which was of great importance in the binding and interaction between flavonoids and the protein. The decreased affinity could be realized by the methoxylation (C8, C3’ and C4’) and glycosylation (C3 and C7) of flavonoid type I. However, the adverse trend on binding affinity was observed when the methoxylation and glycosylation appeared at the sites C4’ and C7, C4’ of structure type II, respectively. Meanwhile, glycosylation at C7 mainly induced the decline in the affinity of flavonoids (type III), and the hydrogenation of the C2 C3 double bond for type I was beneficial to increase the affinity on BSA. Moreover, part of flavonoids could mediate the conformational alteration of secondary structures of the protein during the interaction process, which was inferred by means of the synchronous fluorescence spectra . The determinations of ANS fluorescence probe suggested that hydrophobic interaction played an important role in the binding of a majority of flavonoids to BSA. Further evidences from the site-specific experiments revealed that the location of flavonoids 19 , 29 and 34 binding on BSA mainly belonged to site I, while compound 3 bound to both sites I and II. Additionally, molecular modelling studies further confirmed the indispensable character of hydrophobic interaction and hydrogen bonds, and illustrated the preferred complex binding behaviors.
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FOOD CHEMISTRY
·
2020
·
IF 6.306
Jie Zhao,Lin Huang,Chunyong Sun,Dongsheng Zhao,Hongjin Tang
In this study, some flavonoids were screened as potent xanthine oxidase (XO) inhibitors in vitro . Flavonoid 9 was demonstrated to exhibit the inhibitory activity through a ping-pong mechanism. Further structure-activity relationship revealed that different structural elements had greatly influenced the inhibition effect on XO and underlined the requirement of hydroxyl groups at C5 and C4′ of flavonoid type I. Moreover, some bioactive flavonoids could efficiently quench the intrinsic fluorescence of XO by either static or static-dynamic mixed mechanism. The synchronous fluorescence, ANS-binding fluorescence, Fourier transform infrared spectra and circular dichroism suggested that active flavonoids could bind to the active center of XO, prevent the entrance of substrate, and induce the rearrangement and conformation change of its secondary structures, ultimately resulting in the significant inhibition effect. Additionally, molecular docking further confirmed these conclusions and highlighted the great importance of hydrophobic interactions and hydrogen bonds for the formation of stable complex conformation.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Yuhan Liu,Yu Lei,Luorui Shang,Zhaomin Yu,Li Wang,Jiabin Zhou,Guangtao Pan
Ethnopharmacological relevance Dachengqi Decoction (DCQD), a traditional formula from Treatise on Exogenous Febrile Disease, purges heat and preserves Yin. Previous studies indicate its efficacy against sepsis-associated liver injury (SALI), but its active components and mechanism remain unclear. Aim of the study To elucidate the effective components and mechanism of DCQD on SALI through network pharmacology and experimental validation. Materials and methods Active compounds and targets of DCQD were screened via TCMSP, while SALI-related targets were obtained from DisGeNET, OMIM, and GeneCards. A "DCQD-compounds-targets-disease" network was constructed using Cytoscape, followed by GO/KEGG enrichment analysis. The compound-target interactions were assessed via molecular docking. The protective effect and mechanism of DCQD on SALI were validated in vivo. Results 35 compounds and 194 related targets of DCQD were screened, 508 genes were predicted as SALI related targets. 62 potential therapeutic targets of DCQD for SALI were selected, in which 11 hub targets were obtained. GO and KEGG analysis implicated multiple signaling mechanisms. Molecular docking confirmed strong binding between key compounds and targets. In SALI mice, DCQD reduced inflammatory cytokines, oxidative stress, apoptosis, and improved intestinal barrier function. Mechanistically, DCQD exerted hepatoprotective effects by activating the PI3K/AKT signaling pathway. Conclusions DCQD alleviated SALI by suppressing inflammation, oxidative stress, apoptosis, and enhancing intestinal barrier integrity via the activation of PI3K/AKT pathway.
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JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Ruoxue Bai,Manling Li,Yingyi Zhang,Jiangwei He,Yujin Lin,Haoran Zhang,Ping Shu,Xiaojing Yan,Bo wang,Tingting Huang,Xue Zhao,Xinfeng Zhao
Immobilized protein-based affinity chromatography offers a promising alternative for high-throughput drug screening, yet its effectiveness is often hampered by conventional immobilization techniques as they result in functional loss of the protein due to random orientation and multi-step purification. Herein, we developed a universal strategy for fabricating highly efficient chromatographic methods by integrating genetic code expansion with alkyne-azide click cycloaddition in the immobilization of dopamine D2 receptor (DRD2) and glucocorticoid receptor (GR) strain-promoted alkyne-azide cycloaddition (SPAAC). The site-specific incorporation of p -azidophenylalanine ( p AzF) into the two receptors using an engineered E. coli C321.ΔA strain enabled direct, oriented, and covalent immobilization of them onto dibenzocyclooctyne (DBCO)-functionalized silica by a copper-free click reaction without the need for prior purification. The resulting immobilized DRD2 p AzF and GR p AzF stationary phases were thoroughly characterized by XPS, SEM/EDS, and immunofluorescence, confirming successful immobilization and preserved receptor activity. These exhibited exceptional specificity, stability (>30 days), and reproducibility (RSD<1%). Applications immobilized DRD2 and GR in analyzing Wendan Decoction identified liquiritigenin, neohesperidin, and naringin as active components of the prescription. Among them, liquiritigenin, neohesperidin were characterized as dual-target ligands. Determination of their binding affinities to the receptors by frontal analysis enables calculation of drug-like efficiency indices (SEI, BEI, LLE). Cellular assays in HT22 cells further confirmed their bioactivities and target specificity to the receptors. This work is possible to advance the discovery of potential leads from natural products as it has the properties of streamlining the construction of reliable receptor chromatography methods, integrating the screening with binding affinity measurement, and preliminary drug-likeness evaluation.
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