Jove-Journal of Visualized Experiments
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2021
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IF 1.355
Jie Ding,Rui Yan,Luna Wang,Qianlei Yang,Xiaoyun Zhang,Nan Jing,Yuanjie Wei,Hengdong Zhang,Yan An
Chemically induced bone loss due to lead (Pb) exposure could trigger an array of adverse impacts on both human and animal skeletal systems. However, the specific effects and mechanisms in zebrafish remain unclear. Alizarin red has a high affinity for calcium ions and can help visualize the bone and illustrate skeletal mineral mass. In this study, we aimed to detect lead acetate (PbAc)-induced bone loss in zebrafish larvae by using alizarin red staining. Zebrafish embryos were treated with a series of PbAc concentrations (0, 5, 10, 20 mg/L) between 2 and 120 h post fertilization. Whole-mount skeletal staining was conducted on larvae at 9 days post fertilization, and the total stained area was quantified using ImageJ software. The results indicated that the mineralized tissues were stained in red, and the stained area decreased significantly in the PbAc-exposure group, with a dose-dependent change in bone mineralization. This paper presents a staining protocol for investigating skeletal changes in PbAc-induced bone defects. The method can also be used in zebrafish larvae for the detection of bone loss induced by other chemicals.
研究领域:
毒理学
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发育生物学
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环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2022
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IF 4.52
Yikun Cui,Zhen-quan Yang,Lixia Xiao,Ming Yang,Xiaojuan Gong,Lizhen Liu,Jie Han,Qin Hu
In this study, an ultrasensitive fluorescence method based on bright orange-emissive carbon quantum dots (OE-CQDs) was for the first time developed for the detection of new coccine (NC). The OE-CQDs were synthesised by a one-pot solvothermal method with saffron T as precursor. The detection mechanism was based on the interaction between OE-CQDs and NC, where the fluorescence of OE-CQDs was inhibited by NC via inner filter effect (IFE) and static interaction. The OE-CQDs probe exhibited high selectivity and good anti-interference ability towards NC sensing. A linear relationship with the concentration of NC in a range of 0.01–20.0 µmol L −1 and a detection limit as low as 1.8 nmol L −1 were obtained. The developed fluorescence method was successfully applied for NC determination in commercial food samples, including dried waxberry, haw flakes, watermelon frost lozenges, strawberry jam and energy drink, with satisfactory results were achieved. The recoveries were in a range of 97.0–104.0% and the relative standard deviations (RSDs) were less than 3.74%. This work highlights the great potential of using long-wavelength emissive CQDs as nanoprobe to achieve enhanced sensitivity in food additives sensing.
研究领域:
分析化学
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食品安全
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纳米技术
✓ 已验证
DOI
高影响因子
DYES AND PIGMENTS
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2022
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IF 5.122
Yujiao Tu,Ze Liu,Lei Jiang,Yingying Xiang,Fei Song,Lifen Meng,Xiuling Ji,Lin Yuan
New coccine is one of the most used red colorants in foods. Due to its cytotoxicity, quantitative determination is very necessary. Herein, the ratiometric fluorescent and UV absorption dual-mode nanosensor , derived from fangchinoline and o-phenylenediamine, was successfully prepared by hydrothermal way. The prepared carbon dots with three colors were purified with dialysis, thin layer chromatography and chromatographic column. Highly green fluorescence of Fan,N-CDs was quenched by new coccine with inner filter effect. The dependence of the ratiometric fluorescent response on new coccine concentrations was found linear over the range of 0–12.5 μM and 20–55 μM with LOD of 30.12 nM and 54.85 nm, respectively. The linearity of UV absorption ranged from 0 to 45 μM with of LOD 1.98 nM. In addition, the nanosensor was used to determine new coccine in energy drinks, carbonated beverage, gum and ketchup. It provides a new insight on the detection of new coccine by the dual-mode nanosensor.
研究领域:
分析化学
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食品安全
·
生物传感
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Lei Cheng,Rong Huang,Qiang Cao,Na Liu,Peiyi Li,Mengmeng Sun,Haiyang Qin,Lidong Wu
Azo pigments with azo functional groups (–N N–) are commonly used in foods. Through rational design, here we report synthesis of a novel magnetic metal–organic framework (MOF-545) and the use of Fe 3 O 4 @MOF-545 for the selective separation of azo pigments from beverages. We find that Fe 3 O 4 @MOF-545 exhibits excellent adsorption/removal capacities for new coccine (NC, 459 mg g −1 ) and lemon yellow (LY, 476 mg g −1 ) individually. The results indicated that the adsorption capacities of Fe 3 O 4 @MOF-545 were 5 times higher than reported previously. The large surface area (120.4 m 2 /g) and the suitable pore size (1.6 nm) of Fe 3 O 4 @MOF-545 provided multiple accessible channels for azo pigment adsorption. The adsorption kinetics, stability, recovery, and reusability of Fe 3 O 4 @MOF-545 were all reported. The recoveries ranged from 92.9 to 104.5%, reflecting the capacity of MOF-545 to efficiently separate NC and LY. Fe 3 O 4 @MOF-545 is suitable for azo pigment determination and separation in food matrices.
研究领域:
材料科学
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分析化学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2016
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IF 4.219
Kai Liu,Lihui Chen,Liulian Huang,Yaoneng Lai
A multi-functional adsorbent was prepared by modifying nanofibrillated cellulose/chitosan composites with ethylenediamine (E-NFC/CS). The E-NFC/CS was characterized by FTIR and used for adsorption of cationic dye methylene blue (MB) and anionic dye new coccine (NC) from aqueous solution. The FTIR results showed that the E-NFC/CS contained more amino groups than the NFC/CS due to the modification for the NFC/CS with ethylenediamine. The results indicated that the maximum adsorption capacities occurred at pH 4.0 for MB and pH 2.0 for NC, respectively. The adsorption equilibrium time for MB and NC was 30 and 50 min, respectively. In addition, the regenerated E-NFC/CS exhibited excellent adsorption performance for NC. It can keep almost 98% of the adsorption capacity after reused three times. Therefore, the E-NFC/CS can be potentially used as an effective adsorbent of cationic and anionic dyes in industrial effluents.
研究领域:
环境科学
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材料科学
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化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）