SENSORS
·
2023
·Vol. 23
·期 2
·695
·
IF 3.9
Zhipeng Yu,Huan Wu,Zhongshuang Xu,Zhimao Yang,Jian Lv,Chuncai Kong
Designing highly active material to fabricate a high-performance noninvasive wearable glucose sensor was of great importance for diabetes monitoring. In this work, we developed CuxO nanoflakes (NFs)/Cu nanoparticles (NPs) nanocomposites to serve as the sensing materials for noninvasive sweat-based wearable glucose sensors. We involve CuCl2to enhance the oxidation of Cu NPs to generate Cu2O/CuO NFs on the surface. Due to more active sites endowed by the CuxO NFs, the as-prepared sample exhibited high sensitivity (779 μA mM−1cm−2) for noninvasive wearable sweat sensing. Combined with a low detection limit (79.1 nM), high selectivity and the durability of bending and twisting, the CuxO NFs/Cu NPs-based sensor can detect the glucose level change of sweat in daily life. Such a high-performance wearable sensor fabricated by a convenient method provides a facile way to design copper oxide nanomaterials for noninvasive wearable glucose sensors.Keywords:noninvasive wearable sensing;metal oxide;sweat glucose detection
研究领域:
生物医学工程
·
材料科学
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产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2025
·
IF 14.1
Ziyi Lu,Ruisi Cai,Tianao Xie,Sheng Zhao,Sarun Juengpanich,Win Topatana,Shijie Li,Jiasheng Cao,Jiahao Hu,Tianen Chen,Jiachen Chen,Jicheng Yu,Tao Sheng,Wentao Zhang,Hao Wang,Jiahuan You,Yukai Shan,Yuchao Sun,Ruijing Shen,Zhengjie Zhao,Kangfan Ji,Ziqi Gao,Xinmin Yu,Xiujun Cai,Zhen Gu,Yuqi Zhang,Mingyu Chen
Monitoring neutrophil levels plays an essential role in diagnosing infections and various diseases, as well as for managing specific treatments such as chemotherapy and radiotherapy. However, the traditional complete blood count technique is invasive and challenging for quick and frequent monitoring of neutrophil. Herein, a button-like microneedle patch for rapid and convenient detection of neutrophil levels is reported without vascular invasion. This patch consists of a hollow microneedle array with a vacuum chamber for extraction of interstitial fluid, a hydrogel sheet for colorimetric detection of neutrophil level based on neutrophil-specific enzyme (myeloperoxidase), and a mobile phone application for data recording and quantification. The detection ability is validated both in vitro and in vivo in a chemotherapy-induced neutropenic rat model. Moreover, this button patch achieves precise measurement of the neutrophil levels in patients’ urine and abdominal drainage fluid. This minimally invasive colorimetric patch can potentiate in situ neutrophil monitoring without blood sampling procedures or in-lab apparatus, which can serve as a substitution for routine blood counts especially for patients in less developed areas.
研究领域:
医学诊断
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生物工程
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免疫学
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DOI
PubMed
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 2
·209
·
IF 5.719
Aiqing Ma,Hui Ran,Jiaxing Wang,Rui Ding,Chengyu Lu,Lanlan Liu,Yingmei Luo,Huaqing Chen,Ting Yin
Sonodynamic therapy (SDT), as a novel cancer therapy strategy, might be a promising approach due to the depth-penetration property in tissue. Sonosensitizers are the key element for efficient SDT. However, the development of sonosensitizers with strong sonosensitization efficacy is still a significant challenge. Herein, an urchin-shaped copper-based metalloporphyrin liposome nanosystem (FA–L–CuPP) is constructed and identified as an excellent sonosensitizer. Under ultrasound (US) irradiation, FA–L–CuPP can be highly excited to generate several reactive oxygen species (ROS), such as singlet oxygen (1O2) and free radicals (⋅OH). The molecular orbital distribution calculations reveal that a strong intramolecular charge transfer might occur in the CuPP complex under US irradiation, which could afford enough energy to the surrounding O2and H2O to concert1O2, O2−and ⋅OH. Working as “ammunitions”, the largely produced ROS can kill 4T1 tumor cells, effectively inhibiting tumor growth. This work provides an urchin-shaped nanosonosensitizer based on a copper complex, which might provide an idea to design a novel sonosensitizer for noninvasive and precise SDT antitumor applications.Keywords:sonodynamic therapy;copper-based metalloporphyrin;sonosensitizer;urchin-shaped;noninvasive therapy
研究领域:
癌症治疗
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材料科学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Ma Li,Pan Jinbin,Shu Gang,Pan Haiyan,Li Jingang,Li Dong,Sun Shaokai
Enhanced computed tomography (CT) imaging with iodinated imaging probes is widely utilized for the diagnosis and evaluation of various liver diseases. However, these iodine-based imaging probes face intractable limitations including allergic reactions and contraindications. Herein, we propose the utilization of renal-clearable iodine-free bismuth chelate (Bi-DTPA dimeglumine) for the non-invasive fast assessment of hepatic ischemia–reperfusion injury (HIRI) via CT imaging for the first time. Bi-DTPA dimeglumine offers several advantages such as simple synthesis, no purification requirement, a yield approaching 100%, large-scale production capability (laboratory synthesis > 100 g), excellent biocompatibility and superior CT imaging performance. In a normal rat model, the administration of Bi-DTPA dimeglumine resulted in a significant 63.79% increase in liver CT value within a very short time period (30 s). Furthermore, in a HIRI rat model, Bi-DTPA dimeglumine enabled the rapid differentiation between healthy and injured areas based on the notable disparity in liver CT values as early as 15 min post-reperfusion, which showed a strong correlation with the histopathological analysis results. Additionally, Bi-DTPA dimeglumine can be almost eliminated from the body via the kidneys within 24 h. As an inherently advantageous alternative to iodinated imaging probes, Bi-DTPA dimeglumine exhibits promising prospects for application in liver disease diagnosis.
研究领域:
医学成像
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肝病学
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放射学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2022
·
IF 5.304
Yaqing Yu,Xiaoyi Jin,Ling Shen,Jin Wang,Jing Lei,Hui Zhang,Juan Zhang
Surface-enhanced Raman scattering (SERS) has proven to be a powerful diagnostic technique for conservation science. In this work, a novel swab-based detection strategy is proposed for minimally invasive SERS analysis of synthetic organic dyes in the cultural heritage field, employing flexible paper-based SERS probes prepared with a facile, cost-efficient and reproducible recipe. It was demonstrated that Ag nanostructures/paper probes composed of interconnected Ag nanostructures immobilized on unmodified cellulose paper showed excellent SERS enhancement for the investigated synthetic organic dyes. The high flexibility and conformability of paper-based substrate allows to extract minimal amounts of dyes from the work of art by gently swabbing in a dry-state condition, thereby avoiding potential damage to colorants from close contact with solvents such as water. Dyes could be transferred via swab-based extraction method without removing any visible fragments of the paint layer, which facilitates preserving the physical integrity of the artwork. SERS detection was subsequently performed directly on flexible substrate surfaces after micro-extraction of dyes. The minimally invasive nature of this strategy has further been confirmed by the spectrophotometric color measurements and successfully applied to the identification of synthetic organic dyes on real paper artworks---Chinese woodblock New Year paintings from the late Qing Dynasty. These results highlight the great potential of flexible paper-based Ag substrates based on swab-detection method for minimally invasive analysis of organic dyes in cultural heritage.
研究领域:
文物保护科学
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分析化学
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产品关联
DOI
高影响因子
Cancer Reports
·
2022
·Vol. 6
·期 2
·e1688
·
IF 0
Ying Liang,Yan Liu,Pin Zhang,Mengxin Zhang,Bang Du,Weyland Cheng,Zhidan Yu,Lifeng Li,Huanmin Wang,Guangjun Hou,Xianwei Zhang,Wancun Zhang
The postoperative recurrence of neuroblastoma (NB) patients is an essential reason for the high mortality of NB due to the lack of early, non-invasive, and dynamic strategies for monitoring NB recurrence. Therefore, whether the plasma circulating cell-free MYCN gene as an indicator for monitoring of NB recurrence was systematically evaluated. The MYCN copy number and NAGK (reference gene) copy number (M/N) ratio in plasma and corresponding tumor tissues of NB patients was detected using an economical, sensitive, and specific single-tube dual RT-PCR approach developed in this study. The plasma M/N ratio of the MYCN gene amplification (MNA) group ( N = 25, median M/N ratio = 4.90) was significantly higher than that of the non-MNA group ( N = 71, median M/N ratio = 1.22), p < .001. The M/N ratio in NB plasma ( N = 60) was positively correlated with the M/N ratio in NB tumor tissue ( N = 60), with a correlation coefficient of 0.9496. In particular, the results of dynamic monitoring of postoperative plasma M/N ratio of NB patients showed that an abnormal increase in M/N ratio could be detected 1–2 months before recurrence in NB patients. In summary, the single-tube double RT-PCR approach can be used to quantitatively detect MYCN copy number. The copy number of MYCN in the tissue and plasma of NB patients is consistent with each other. More importantly, the circulating cell-free MYCN gene of NB patients can be used as a monitoring indicator for early, non-invasive, and dynamic monitoring of NB recurrence.
研究领域:
肿瘤学
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分子生物学
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遗传学
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DOI
PubMed
ADVANCED FUNCTIONAL MATERIALS
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2022
·Vol. 32
·期 48
·2207879
·
IF 19.924
Lexiang Zhang,Wenzhao Li,Rokshana Parvin,XiaoChen Wang,Qihui Fan,Fangfu Ye
Exosomes are cell-derived extracellular vesicles which enclose genetic substances from their origin cells, offering a good source of biomarkers. Many efforts have been made to develop non-invasive exosome-based clinical diagnosis, which are urgently needed for prostate cancer, as traditional methods are criticized due to the inaccuracy and discomfort. In this study, a simple, universal process for genotyping of exosome-derived microRNAs using nanoparticles to capture exosomes and the novel photothermal droplet digital polymerase chain reaction (ddPCR) assay to detect target molecules is presented. To detect the target sensitively and simply, the smart ddPCR thermocycling of the agarose microcarriers can be controllably realized via embedding photothermal MXene and magnetic nanoparticles composite, and then irradiating with a homemade automated near-infrared control module. Duplex photothermal-driven ddPCR cooperating with dual TaqMan probes enabled simultaneously and quantitatively detecting copy numbers of prostate oncogenes microRNA-21-5p, microRNA-375-3p, and metastasis microRNA-574-3p along with the control microRNA-30a-5p within the extracted exosomes. The results show that the expression levels of these biomarkers in prostate cancer cell lines PC-3 and DU145 are 7–210 folds higher than those in healthy control cells HaCaT and HUVEC. Thus, it is believed that these versatile exosomal microRNA genotyping approaches are potentially valuable for diverse clinical applications.
研究领域:
癌症生物学
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分子诊断
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纳米技术
·
生物医学工程
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产品关联
DOI
高影响因子
Journal of Innovative Optical Health Sciences
·
2024
·
IF 2.3
Jiarui Wang,Shiyan Jiang,Jiaxin Shi,Jing Wang,Shengrong Du,Zufang Huang
Male infertility affects 10–15% of couples globally, with azoospermia — complete absence of sperm — accounting for 15% of cases. Traditional diagnostic methods for azoospermia are subjective and variable. This study presents a novel, noninvasive, and accurate diagnostic method using surface-enhanced Raman spectroscopy (SERS) combined with machine learning to analyze seminal plasma exosomes. Semen samples from healthy controls ( n = 3 2 ) and azoospermic patients ( n = 2 2 ) were collected, and their exosomal SERS spectra were obtained. Machine learning algorithms were employed to distinguish between the SERS profiles of healthy and azoospermic samples, achieving an impressive sensitivity of 99.61% and a specificity of 99.58%, thereby highlighting significant spectral differences. This integrated SERS and machine learning approach offers a sensitive, label-free, and objective diagnostic tool for early detection and monitoring of azoospermia, potentially enhancing clinical outcomes and patient management.
研究领域:
生殖医学
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生物医学工程
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分析化学
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医疗健康中的机器学习
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DOI
高影响因子
Advanced Intelligent Systems
·
2021
·Vol. 3
·期 4
·2000090
·
IF 0
Shengnan Chen,Heng Zhang,Xiangfei Yang,Xiaoguang Shao,Taihao Li,Na Chen,Zhenyi Chen,Wei Xue,Jiahua Pan,Shupeng Liu
Early diagnosis of prostate cancer (PCa) is always a great challenge in clinical practice, especially in distinguishing benign prostatic hyperplasia (BPH) from early cancer, due to the high similarity in pathology from the prostate-specific antigen (PSA) test and radiological detection. The conventional diagnostic methods are often less efficient in specificity and accuracy, leading to quite a few unnecessary biopsies. This work establishes a noninvasive diagnostic method for PCa by investigating urine samples using Raman spectroscopy and convolutional neural network (CNN) algorithm. The results of urine Raman spectra show the intensities of characteristic peaks for lipids, nucleic acids, and some amino acids are distinguishable between PCa and BPH, suggesting an abnormal metabolism caused by PCa, which can be detected by Raman spectroscopy. These data are then used to train an intelligent diagnostic model with CNN algorithm. The cross-validation results show the mean diagnostic accuracy, sensitivity, and specificity for PCa are 74.95%, 77.32%, and 72.46%, respectively. This noninvasive diagnostic method is a promising method for the early diagnosis of PCa, and the idea of using urine Raman spectroscopy with deep learning techniques for diagnosing PCa provides a reference for the application of artificial intelligence in the field of clinical medicine research.
研究领域:
肿瘤学
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泌尿科学
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分子诊断学
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转化医学
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生物光子学
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临床化学
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人工智能医学
✓ 已验证
DOI
ACS Sensors
·
2022
·
IF 9.618
Xiaoping Yue,Feiyang Xu,Linrong Zhang,Guozhang Ren,Huixiang Sheng,Jin Wang,Kaili Wang,Liuyingzi Yu,Junjie Wang,Gongqiang Li,Gang Lu,Hai-Dong Yu
In situ analysis of sweat provides a simple, convenient, cost-effective, and noninvasive approach for the early diagnosis of physical illness in humans and is particularly useful in family care. In this study, a flexible and skin-attachable colorimetric sweat sensor for multiplexed analysis is developed using a simple, cost-effective, and convenient method. The obtained sweat sensor can be used to simultaneously detect glucose, lactate, urea, and pH value in sweat, as well as sweat loss and skin temperature. Only 2.5 μL of sweat is enough for the whole test, and the sweat loss and chemical-sensing results can be read out conveniently by naked eyes or a smartphone. In addition, body temperature can also be detected with an additional electrical circuit. Our sweat sensor provides a new, cost-effective, and convenient approach for in vitro diagnosis of multiple components in sweat, and the easy fabrication and cost-effectiveness make our sensor commercializable in the near future.
研究领域:
生物医学工程
·
分析化学
·
医疗诊断
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Fang Wang,Jingfang Li,Huiting Xia,Jiaqi Liu
Non-invasive, continuous monitoring of sweat biomarkers using flexible sensors enables real-time tracking of indicators such as glucose, electrolytes, and lactate, facilitating early disease detection and improved health management. In this paper, a flexible screen-printed electrode (labeled as Cu@PHEMA/SPE) based on a Cu 2+ doped poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogel was prepared by a simple free radical polymerization method, which is used for glucose and ascorbic acid detection in sweat. This hydrogel exhibits excellent mechanical properties (184 kPa and 346 %), self-healing capabilities and recycled self-adhesive performance. Cyclic voltammetry (CV) and chronoamperometry techniques were employed to investigate the electrochemical properties of the hydrogel electrode in an alkaline solution and the flexible electrode in a neutral solution, with respect to glucose and ascorbic acid. The flexible electrode demonstrated a wide linear range, high sensitivity, and a low detection limit. Specifically, glucose detection ranged from 0 to 2 mM, exhibiting sensitivities of 122.24 and 24 μA/mM/cm 2 , with a detection limit of 4.5 μM. Moreover, it can detect ascorbic acid in the range of 0–220 μM, with sensitivities of 799.85 and 201.94 μA/mM/cm 2 , and a detection limit of 0.96 μM. Furthermore, the electrode displayed good selectivity for interfering substance such as uric acid (UA), dopamine (DA), lactic acid (LA), sodium chloride (NaCl), and potassium chloride (KCl), and it maintained performance despite external deformation. Notably, real sweat samples analysis demonstrated strong concordance with the results obtained using commercially available kits. These results indicate that this flexible electrode has great potential for application in wearable devices.
研究领域:
生物医学工程
·
电化学传感
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可穿戴设备
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即时检测
·
代谢物监测
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产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 32
·期 6
·2107624
·
IF 18.808
Tingting Li,Heng Guo,Ye Liu,Weizhi Qi,Changfeng Wu,Lei Xi
Both treatment and postoperative evaluation of cancer play vital roles in improving the survival rate of patients. However, existing cancer therapies face multiple challenges of low drug delivery efficiency, separation of treatment and evaluation, and lack of noninvasive, routine and cost-efficient postoperative monitoring methods. To overcome these issues, an all-in-one anti-cancer strategy is proposed by using the combination of the photoacoustic (PA) effect and polymer nanoparticles. This strategy shows enhanced nanoparticles delivery, significant tumor ablation, and the capability of non-invasive and longitudinal postoperative evaluation. Near-infrared (NIR) excited photoacoustic radiation force (PAF) based on the high optical absorption nanoparticles opens the vascular barrier, accumulates the nanoparticles inside the tumor, and enables visualization of real-time nanoparticles distribution. Instead of conventional thermal therapy by using a continuous wave laser, the NIR pulsed laser coupled with PAF offers thermal therapy with further positive accumulation of nanoparticles, and significantly improves the treatment efficiency in return. In addition, the endogenous hemoglobin enables PA imaging to evaluate the efficacy of tumor therapy and monitor the progress of postoperative tumors in vivo through structural and functional parameters of the vascular network. This study presents a new opportunity for formulation of personalized and accurate tumor therapy, and timely feedback of therapeutic effect.
研究领域:
生物医学工程
·
纳米技术
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癌症研究
·
诊疗一体化
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）