RSC Advances
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2019
·Vol. 9
·期 18
·9893-9898
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IF 3.049
Qingju Liu,Ping Han,Hui Wang,Wenwen Gong,Xiaoyuan Feng
Currently known rapid determination of fungicides usually relies on antibody-based immunoassay. This paper reports a simple antibody-free colorimetric assay for chlorothalonil via the inhibition of an enzyme-triggered reaction. The enzymatic activity of glyceraldehyde-3-phosphate dehydrogenase was significantly inhibited by chlorothalonil, and the color change of NBT-PMS system induced from NADH formation was suppressed, which could be used indirectly to assay chlorothalonil. The limit of detection (LOD) was 0.05 μM with a linear range from 0.5 to 10 μM, and the detection of 1 μM chlorothalonil in solution was achieved with a naked-eye readout. In addition, the colorimetric measurement results of the cucumber samples showed a good recovery rate, although the sensitivity was less effective than the instrumental method. Nevertheless, the results demonstrates that the chlorometric method provides potential opportunities for reliable, cost-effective quantitative detection for chlorothalonil residues in vegetables.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
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2023
·Vol. 13
·期 6
·647
·
IF 5.4
Jingbo Zhang,Quancheng Chen,Xuemin Gao,Qian Lin,Ziqin Suo,Di Wu,Xijie Wu,Qing Chen
In this study, a label-free and antibody-free impedimetric biosensor based on molecularly imprinting technology for exosomes derived from non-small-cell lung cancer (NSCLC) cells was established. Involved preparation parameters were systematically investigated. In this design, with template exosomes anchored on a glassy carbon electrode (GCE) by decorated cholesterol molecules, the subsequent electro-polymerization of APBA and elution procedure afforded a selective adsorption membrane for template A549 exosomes. The adsorption of exosomes caused a rise in the impedance of the sensor, so the concentration of template exosomes can be quantified by monitoring the impedance of GCEs. Each procedure in the establishment of the sensor was monitored with a corresponding method. Methodological verification showed great sensitivity and selectivity of this method with an LOD = 2.03 × 103and an LOQ = 4.10 × 104particles/mL. By introducing normal cells and other cancer cells derived exosomes as interference, high selectivity was proved. Accuracy and precision were measured, with an obtained average recovery ratio of 100.76% and a resulting RSD of 1.86%. Additionally, the sensors’ performance was retained at 4 °C for a week or after undergoing elution and re-adsorption cycles seven times. In summary, the sensor is competitive for clinical translational application and improving the prognosis and survival for NSCLC patients.Keywords:exosomes;molecular imprinted polymers (MIPs);antibody-free;impedimetric sensor
研究领域:
生物传感器
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癌症诊断
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纳米技术
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电化学
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产品关联
DOI
PubMed
高影响因子
Materials Today Nano
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2022
·
IF 13.364
X. Cui,Y. Zhou,Y. Zheng,L. Cao,L. Gao,J. Duan,H. Yin,S. Ai
N 6 -methyladenosine (m 6 A) is the most abundant and dynamic reversible modification of mRNA in eukaryotes discovered in the mid-1970s, which is widely involved in various physiological processes. Fat mass and obesity-associated protein (FTO protein) is not only a demethylase for m 6 A, but also a carcinogenic factor for many human cancers. Therefore, it is very important to build a simple and sensitive platform for simultaneous detection of m 6 A and FTO protein. To achieve this aim, a novel photoelectrochemical biosensor was constructed with the antibody-free detection strategy of DTT mediated –SH functionalization of m 6 A triggered by FTO protein catalyzed m 6 A oxidation . To achieve high detection sensitivity, CdS/Bi 2 MoO 6 /MoSe 2 system with matched energy gap was employed as photoactive material. To achieve high detection selectivity , the –CH 3 of m 6 A in the captured RNA sequence (m 6 A RNA) on the electrode surface was oxidized by FTO protein to form hydroxymethyl, followed with the covalent reaction of DTT with hydroxymethyl, making the labeling of m 6 A with –SH. Finally, a CdS nanoparticle was modified on the electrode through the specific covalent reaction between –SH and Cd, achieving the improvement of the photocurrent with the matched energy band . Under the optimal experimental conditions, the biosensor showed linear range from 0.001 to 50 nM and 0.0005–500 μg/L with the detection limit of 0.37 pM and 0.034 ng/L (S/N = 3) for m 6 A RNA and FTO protein, respectively. The applicability of the developed method was assessed by investigating the effect of entacapone on FTO protein activity.
研究领域:
生物化学
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分析化学
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生物技术
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癌症研究
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Pengcheng Wu,Wanchao Zuo,Yufeng Wang,Qinfang Yuan,Jun Yang,Xinmei Liu,Hui Jiang,Jianjun Dai,Feng Xue,Yanmin Ju
Bacterial urinary tract infections cause huge challenges to public health and require rapid diagnostic methods, while lateral flow immunoassay (LFIA) belongs to the most known point-of-care detection technology. Capture-antibody (CA) is used for labeling targets in traditional LFIA, yet the production of antibodies is time-consuming and expensive. In this study, we reported a multimodal CA-independent LFIA (MCI-LFIA) method for rapid diagnosis of bacterial urinary tract infections with high flexibility and accuracy. The assay employs p-mercaptophenylboronic acid-modified Au nanoflower (namely AuNF-PMBA nanomaterials) as multifunctional labels, which possess colorimetric-Raman-photothermal properties and outstanding bacteria capture ability. Take Escherichia coli ( E. coli ) as model bacteria, the limit of detection (LOD) of this MCI-LFIA for E. coli was 10 3 cfu/mL by naked-eye, which is three orders of magnitude lower than conventional CA-dependent sandwich method. For quantitative detection, LOD was 10 3 cfu/mL in colorimetric mode, 10 2 cfu/mL in Raman mode and 10 2 cfu/mL in photothermal mode. Significantly, the developed AuNF − PMBA-based MCI-LFIA successfully detected target pathogens in clinical E. coli -positive human urine samples within 45 min with high accuracy. This diagnostic platform may be easily adapted for detection of other target pathogens for bacterial diseases in point-of-care settings with good reproducibility and excellent specificity.
研究领域:
医学诊断
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纳米技术
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即时检测
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尿路感染
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Xin Liu,Dawei Fan,Chenchen Zhang,Xinrong Shao,Hongmin Ma,Yong Zhang,Xueying Wang,Qin Wei,Huangxian Ju
An ultrasensitive photoelectrochemical (PEC) antifouling immunosensor was proposed using BiOBr/FeTPPCl/BiOI as matrix and polyacrylic acid (PAA)/polyethylene glycol (PEG) as hybrid antifouling interface for MDA-MB-231 detection. An indium tin oxide (ITO) electrode was successively modified with bismuth oxybromide (BiOBr) with layered crystal structure, which was sensitized with tetraphenyl iron(Ⅲ) porphyrin chloride (FeTPPCl) to widen the absorption range of visible light, and decorated with bismuth oxyiodide (BiOI) nanosheets to improve the PEC response. The joint action of PAA and PEG could offset the adsorption of non-specific proteins on the immunosensing interface for obtaining good antifouling performance. Based on the specific interaction of sialic acid (SA) on the cytomembrane with 3-aminophenylboronic acid (APBA), and the synergistic antifouling effect of PEG and PAA, a simple PEC immunosensing method was developed for the quantitative determination of MDA-MB-231 cells. This proposed antifouling PEC immunosensor exhibited a wide linear range (1 × 10 2 ∼ 1 × 10 6 cells·mL -1 ) and a low detection limit (30 cells·mL -1 ). The development of antibody-free immunosensors may improve the application for the diagnosis of human breast cancer.
研究领域:
材料科学
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分析化学
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生物化学
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癌症诊断
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DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 12
·1-9
·
IF 5.479
Feng Jun,Li Xuan,Cheng Hao,Huang Wenyi,Kong Hongxing,Li Yanqing,Li Lijun
An antibody-free immunoassay that makes use of a boronate affinity molecularly imprinted polymer (MIP) and surface enhanced Raman scattering (SERS) tags is described. It was applied to the specific determination of the carcinoembryonic antigen (CEA) in human serum. For the preparation of the boronate affinity array, a polymer capable of adsorbing glycoproteins was first synthesized on the surface of a glass slide with four spots using 4-vinylbenzeneboronic acid (VPBA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the crosslinking agent, and ethylene glycol and cyclohexanol as porogens. The surface of the VPBA-Co-EGDMA can bind target glycoproteins. After specific capture of the glycoprotein, a “MIP-target glycoprotein-SERS tag” sandwich structure was formed by covalent interaction between the SERS nanotag (consisting of gold nanoparticles and 4-mercaptophenylboronic acid [MPBA]). CEA can be quantified in spiked serum with a detection limit of 0.1 ng·mL −1 via the specific Raman band at 1098 cm −1 .
Schematic representation of the boronate affinity molecularly imprinted polymer (MIPs) array-based SERS sensor for rapid and sensitive detection of the carcinoembryonic antigen (CEA) from human serum. The boronate affinity MIPs array are used as capture probes, and MPBA@AuNPs are used as SERS tags.
研究领域:
分析化学
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生物技术
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生物传感器
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癌症研究
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）