Yang You,Bin Luo,Cheng Wang,Hongtu Dong,Xiaodong Wang,Peichen Hou,Lijun Sun,Aixue Li
Gibberellins (GA 3 ) is an ubiquitous plant hormone, which plays a regulatory role in different growth stages of plants, so it is of great significance to develop a sensitive quantitative analysis method for GA 3 . In this study, carboxylated graphene oxide- carboxylated multi-walled carbon nanotubes-Fc (GO-MWNT-Fc) composite material and PDANPs-antibody (PDANPs-Ab) were sequentially modified to screen-printed electrodes (SPEs), and an ultrasensitive probe-free immunosensor for GA 3 was developed. Fc was applied to generate electrochemical signals. GO-COOH and MWNT-COOH can increase the catalytic ability of the sensor and bind the PDANPs-Ab nanoparticles. PDANPs nanomaterial were synthetized by a facile self-polymerization and used to bind with antibody, so as to increase the antibody loading of the sensor. The as-prepared immunosensor has the widest detection range (100 aM-1 mM) and lowest detection limit (17.4 aM) for GA 3 up to date. To our knowledge, it is the first electrochemical immunosensor for GA 3 . By changing the GA 3 antibody to ABA antibody, a sensitive and selective immunosensor for ABA was also fabricated. This immunosensor platform is simple, sensitive, and low cost. It opens broad prospect in on-site applications for biosensors in detecting of various biomolecules in precision agriculture.
Gengqi Xing,Cheng Wang,Ke Liu,Bin Luo,Peichen Hou,Xiaodong Wang,Hongtu Dong,Jianshu Wang,Aixue Li
Methyl jasmonate (MeJA) is an important phytohormone which can regulate plant growth and stress tolerance. It is very necessary to develop sensitive and accurate detection methods for MeJA. In this work, a probe-free electrochemical immunosensor for MeJA detection was developed based on a Cu-MOF–carboxylated graphene oxide (COOH-GO) platform. The Cu2+ in the Cu-MOFs was used to provide redox signals, which avoids the application of an external redox probe in the electrolyte solutions as conventional immunosensors. COOH-GO was used to improve the structural stability and provide more sites for binding MeJA antibodies. The linear range of the MeJA immunosensor is from 10 pM to 100 μM, which can cover the whole concentration range of MeJA in most plants. And its detection limit is very low (0.35 pM), and it can detect very low concentrations of MeJA. This immunosensor is simple, low cost, and does not need redox probe solutions for measurements. It shows remarkable potential for on-site application in precision agriculture.
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