ANALYTICAL CHEMISTRY
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2025
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IF 6.7
Lu huang,Hong Jiang,Bin Li,Lanying Chen,Tongtong Yang,Jiang Wang,Huanwen Chen
An online reactive internal extraction electrospray ionization (iEESI) method was developed for the rapid determination of organic and inorganic speciation information for selenium in poultry tissue samples without complex sample pretreatment. The addition of citric acid as a reducing agent to the internal extraction solvent of methanol/acetic acid (99:1, V/V) for iEESI resulted in the reduction of selenate in the sample to selenite, accompanied by the production of malic acid as an oxidation product. The quantitative analysis of selenate was conducted by using malic acid. By addition of o-phenylenediamine (OPD) to the extractant, inorganic selenium including selenite and selenite reduced from selenate was immediately extracted and converted into 1,3-dihydro-2,1,3-benzoselenadiazole after an in situ reaction. The methanol in the reagent extracted organic selenium compounds from the samples, enabling a rapid quantitative analysis of organic and inorganic selenium in chicken tissue. The concentrations of selenomethionine (SeMet), l-selenocystine (SeCys(2)), selenite, and selenate in different tissue samples were examined, showing strong linearity in the range of 1.00–100.00 μg/kg with the limit of detection (LOD) ranging from 0.36 to 0.84 μg/kg. The results were validated by two conventional methods with an accuracy of 84.2–107.6%. The quantitative analysis of three chicken visceral samples revealed that the highest concentration of organic selenium was present in chicken livers, while the highest concentration of inorganic selenium was observed in gizzards. The findings may provide a reliable method for studying selenium metabolism pathways in organisms.
研究领域:
分析化学
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营养学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2019
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IF 6.35
Yonggang Ding,Yu Liu,Youliang Chen,Yi Huang,Ying Gao
Gold nanoparticles (AuNPs) are widely used as optical probes in colorimetric detection, thanks to their high molar extinction coefficient. However, sample matrixes of high salinity or strong acidity/alkalinity often break the electrostatic repulsion of AuNPs suspension, or/and the surface functionality of AuNPs, causing strong and unfavorable interferences. Photochemical vapor generation (PVG) is an efficient technique for the sample matrix separation. Besides, it possesses distinct features of green reducing reagent, reduced interferences from concomitant elements, and direct speciation by the assistance of photocatalyst. Herein, we developed a photochemical vapor generation (PVG) method for the green and direct speciation analysis of inorganic selenium (i.e., Se(IV) and Se(VI)), by colorimetric or visual monitoring of unmodified AuNPs. The generated Se species from PVG were directed into the AuNPs solution for a reaction to take place, which produced a specific new absorption band at 600 nm for detection. The experimental parameters, including the concentration of organic acid, the sample flow rate, the concentration of AuNPs, and the flow rate of carries gas, were optimized in detail. Under optimized conditions, the limits of detection (LOD) for Se(IV) and Se(VI) were 0.007 and 0.006 μg mL–1 by UV–vis detection, respectively. It is worth mentioning that 0.08 μg mL–1 Se can induce an obvious color change, which can be directly observed with the naked eye. Relative standard deviations (RSDs) of 4.5% and 4.3% were obtained from seven replicate measurements of 0.15 μg mL–1 Se(IV) and Se(VI) standard solution, respectively. The developed assay has been successfully applied for the speciation of Se in a dietary supplement sample and environmental water samples including lake water, seawater, simulated water reference materials, and tap water.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
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2024
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IF 4.7
Hong Ding,Xuanxiao Lu,Xiaoguo Ji,Shijie Wang,Jiayang Jin,Mengyao Zhao,Xiaofeng Hang,Liming Zhao
Selenium supplements are beneficial to human health, however, concerns regarding the toxicity of inorganic selenium have stimulated research on safer organic compounds. The main objective of this study was to develop a novel glucosamine-selenium compound (Se-GlcN), clarify its structure, and subsequently investigate its oral toxicity and in vitro anti-hepatitis B virus (HBV) activity. Electron microscopy, infrared, ultraviolet spectroscopy, nuclear magnetic resonance and thermogravimetric analyses revealed a unique binding mode of Se-GlcN, with the introduction of the Se-O bond at the C6 position, resulting in the formation of two carboxyl groups. In acute toxicity studies, the median lethal dose (LD 50 ) of Se-GlcN in ICR mice was 92.31 mg/kg body weight (BW), with a 95% confidence interval of 81.88 to 104.07 mg/kg BW. A 30-day subchronic toxicity study showed that 46.16 mg/kg BW Se-GlcN caused livers and kidneys damage in mice, whereas doses of 9.23 mg/kg BW and lower were safe for the livers and kidneys. In vitro studies, Se-GlcN at 1.25 μg/mL exhibited good anti-HBV activity, significantly reducing HBsAg, HBeAg, 3.5 kb HBV RNA and total HBV RNA by 45%, 54%, 84%, 87%, respectively. In conclusion, the Se-GlcN synthesized in this study provides potential possibilities and theoretical references for its use as an organic selenium supplement.
研究领域:
药物化学
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毒理学
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病毒学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2024
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IF 4.8
Furong Wang,Min Yan,Ting Zhang,Zhen Tian,Xinxin Niu,Zimeng Guo,Xian Wang,Xiaoben Li,Wei Song,Yan'e Luo,Yahong Yuan,Tianli Yue
Conversion of inorganic selenium (Se) to its organic form by Saccharomyces cerevisiae ( S. cerevisiae ) has attracted significant attention, for organic Se has higher nutritional value with less negative effects. However, the mechanism of transformation and accumulation of organic Se at the metabolic level remains unclear. We investigated the metabolic response to sodium selenite supplementation in culture medium at 0, 5, 15, and 45 mg/L in two S. cerevisiae strains using an ultra-high performance liquid chromatography-high resolution mass spectrometry-based untargeted method. In total, 455 metabolites were detected, and a separation trend was clearly observed between Se-treated groups and controls. In depth, metabolic pathways involving amino acid, nucleotide metabolism and tricarboxylic acid cycle were significantly affected. Furthermore, high inorganic-Se supplementation effectively decreased the seleno-amino acid proportion in total Se, inhibited cell growth, and decreased glutathione levels. These results provide novel insights into the Se-enrichment process of S. cerevisiae .
研究领域:
微生物学
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代谢组学
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营养学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）