ANALYTICA CHIMICA ACTA
·
2020
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IF 5.977
Haoyue Qin,Yuanqiang Sun,Xin Geng,Keran Zhao,Hongmin Meng,Ran Yang,Lingbo Qu,Zhaohui Li
In this work, a novel bright yellow fluorescent carbon dots (CDs) was synthesized from N-methyl-1,2-phenylenediamine hydrochloride in ethanol solvent through the solvothermal method . The obtained carbon dots had no fluorescence in water, but could specifically light up in the cell which makes the staining process without washing. Interestingly, the imaging process can be performed by simply shaking the culture with the cells for only 1 min, indicating ultrafast and easy to operate fluorescence imaging. Moreover, the carbon dots with abundant amino functional groups had good lysosome targeting properties (the Pearson’s correlation coefficient is 0.92) and could achieve the ultrafast lysosome imaging in cell and zebrafish and monitoring cell apoptosis status. This is the first lysosome targeting carbon dots that allows the combination of a short incubation at the second-level with wash-free process providing great potential for continuous observation lysosome in vivo.
研究领域:
生物医学工程
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细胞生物学
·
荧光成像
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-10
·
IF 5.3
Gao Bao,Ding Yu,Cai Zhihe,Wu Shi,Wang Juan,Ling Na,Ye Qinghua,Chen Moutong,Zhang Youxiong,Wei Xianhu,Ye Yingwang,Wu Qingping
A dual-recognition strategy is reported to construct a one-step washing and highly efficient signal-transduction tag system for high-sensitivity colorimetric detection of Staphylococcus aureus ( S. aureus ). The porous (gold core)@(platinum shell) nanozymes (Au@PtNEs) as the signal labels show highly efficient peroxidase mimetic activity and are robust. For the sake of simplicity the detection involved the use of a vancomycin-immobilized magnetic bead (MB) and aptamer-functionalized Au@PtNEs for dual-recognition detection in the presence of S. aureus. In addition, we designed a magnetic plate to fit the 96-well microplate to ensure consistent magnetic properties of each well, which can quickly remove unreacted Au@PtNEs and sample matrix while avoiding tedious washing steps. Subsequently, Au@PtNEs catalyze hydrogen peroxide (H2O2) to oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) generating a color signal. Finally, the developed Au@PtNEs-based dual-recognition washing-free colorimetric assay displayed a response in the range of S. aureus of 5 × 10 1 –5 × 10 5 CFU/mL, and the detection limit was 40 CFU/mL within 1.5 h. In addition, S. aureus -fortified samples were analyzed to further evaluate the performance of the proposed method, which yielded average recoveries ranging from 93.66 to 112.44% and coefficients of variation (CVs) within the range 2.72–9.01%. These results furnish a novel horizon for the exploitation of a different mode of recognition and inexpensive enzyme-free assay platforms as an alternative to traditional enzyme-based immunoassays for the detection of other Gram-positive pathogenic bacteria. Graphical abstract
研究领域:
生物化学
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纳米医学
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微生物学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Chen Yang,Xin Zhang,Minjing Mo,Guoqing He,Menglu Gao,Tao Jiang,Yihan Ma,Xiaotong Si,Rui You,Fubing Wang,Yi Yang
Human serum albumin (HSA) in blood testing is clinically important in assessing nutrition, liver and kidney function, inflammation, and chronic diseases. However, traditional HSA testing methods require large equipment, high costs and specialized operators, which cannot meet the demand for portable HSA detection. Here we present a filtration-to-enhancement compound microsphere module (F-ECMs), which achieves wash-free and highly sensitive HSA detection. The F-ECMs utilizes tightly linked filtration module and enhancement module to provide wash-free detection while reducing the limit of detection (LoD) of HSA by 2.048-fold. The filtration module utilizes 30 μm encoded microspheres to achieve wash-free detection, and the enhancement module utilizes the focusing and microcavity effects of 8 μm microsphere for fluorescence enhancement. Customized homomorphic filters can further enhance the image signal and achieve highly sensitive HSA detection. Integrated portable device and smartphone app analysis module enables point-of-care testing (POCT). In this study, the LoD of HSA in blood reaches 0.375 mg/mL and the detection range is 0.1 – 100 mg/mL. The detection time is only 20 min. Double-blind detection was performed on 29 real plasma samples from patients, and its accuracy was achieved 93.10%. This platform provides a new wash-free and fluorescence enhancement strategy for portable HSA detection with high specificity and sensitivity.
研究领域:
生物医学工程
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临床检验
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光学检测
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Xiang Ran,Fang Pu,Zhenzhen Wang,Jinsong Ren,Xiaogang Qu
Accurate and facile differentiation of cell types is essential for accurate diagnosis and therapy of diseases. However, it remains challenging due to low specificity, requirement of sophisticated instruments, and tedious operation steps. Herein, a simple, washing- and label-free chemical tongue was constructed for differentiation of cell types. In the array-based sensing platform, DNA-ligand ensembles adsorbed on the surface of MnO 2 nanosheets were used as sensing probes. Instead of aptamers from cell-SELEX, the randomly designed DNA strands were used, offering versatile interactions with cells. The property that MnO 2 nanosheets can be degraded by intracellular glutathione makes the platform avoid the washing step. Eight types of cell lines were distinguished from each other after the data were treated with principal component analysis (PCA). In addition, a 95% of identification accuracy for the randomly selected unknown samples was achieved. The strategy shows an excellent performance not only in distinguishing cell lines but also in the identification of unknown cell samples.
研究领域:
生物化学
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纳米医学
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细胞生物学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-11
·
IF 5.3
Li Xinyi,Fan Yaofang,Gong Hao,Wang Haoran,Ji Yanli,Xu Longqiang,Ma Cuiping,Shi Chao
A signal amplification electrochemical biosensor chip was developed to integrate loop-mediated isothermal amplification (LAMP) based on in situ nucleic acid amplification and methyl blue (MB) serving as the hybridization redox indicator for sensitive and selective foodborne pathogen detection without a washing step. The electrochemical biosensor chip was designed by a screen-printed carbon electrode modified with gold nanoparticles (Au NPs) and covered with polydimethylsiloxane membrane to form a microcell. The primers of the target were immobilized on the Au NPs by covalent attachment for in situ amplification. The electroactive MB was used as the electrochemical signal reporter and embedded into the double-stranded DNA (dsDNA) amplicons generated by LAMP. Differential pulse voltammetry was introduced to survey the dsDNA hybridization with MB, which differentiates the specifically electrode-unbound and -bound labels without a washing step. Pyrene as the back-filling agent can further improve response signaling by reducing non-specific adsorption. This method is operationally simple, specific, and effective. The biosensor showed a detection linear range of 10 2 –10 7 CFU mL −1 with the limit of detection of 17.7 CFU mL −1 within 40 min. This method showed promise for on-site testing of foodborne pathogens and could be integrated into an all-in-one device. Graphical Abstract
研究领域:
生物传感技术
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食品安全检测
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分子诊断学
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DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 26
·3240-3250
·
IF 3.1
Ruihua Tang,Xueyan Yan,Min Li,Aoqi Du,Hui Yang,Huancai Yin,Mingyue Xie
Nucleic acid detection technologies have been widely utilized for various diseases. Conventional laboratory tests are less suitable for use in resource-limited settings as they are time-consuming, high-cost, complex, and heavily dependent on benchtop equipment. Rapid nucleic acid detection methods that consist of rapid nucleic acid extraction steps could overcome these challenges. A paper-based platform has been utilized to develop various rapid nucleic acid extraction methods owing to its cost-effectiveness, portability, and easy-modification. However, the existing paper-based nucleic acid extraction technologies mainly focus on improving the adsorption capacity of nucleic acids without reducing the non-specific adsorption capacity of proteins. In this study, paper-based nucleic acid extraction technology with wash-free, elution-free, and low protein adsorption was developed. The fabrication of paper involves the mixing of polyethylene glycol (PEG)-modified cotton fiber, chitosan (COS)-modified cotton fiber, and cotton fiber to form PEG-modified cotton fiber/chitosan-modified cotton fiber/cotton fiber (PEG-CF/COS-CF/CF) paper by the wet molding method. The result showed that PEG-CF/COS-CF/CF paper has a desirable pore size (23.9 ± 4.03 μm), good mechanical strength (dry: 9.37 Mpa and wet: 0.28 Mpa), and hydrophilicity (contact angle: 42.6° ± 0.36°). NH3+ groups of COS and OH− groups of PEG were observed on its surface and the adsorption efficiency of nucleic acid in TE buffer was 42.48% ± 0.30%. The limit of detection of pure DNA with this PEG-CF/COS-CF/CF paper by qPCR was as low as 25 ng. Additionally, this platform could successfully extract nucleic acid from 30 μL of a saliva sample, highlighting its potential use for clinical sample testing. The proposed paper-based nucleic acid extraction platform shows tremendous potential for disease diagnosis in resource-limited settings.
研究领域:
生物技术
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分子生物学
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诊断技术
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材料科学
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DOI
PubMed
高影响因子
ACS Sensors
·
2023
·
IF 8.9
Sijia Li,Jiayue Shi,Xia Yang,Yanxia Qiao,Yang Jiang,Yaqian Zhou,Yan Li,Chengxiao Zhang
We propose a novel washing-free electrochemiluminescence (ECL) biosensor for the simultaneous detection of two types of N6 methyladenosines-RNAs (m6A-RNAs), which are potential cancer biomarkers, on the basis of binding-induced DNA strand displacement (BINSD). The biosensor integrated a tri-double resolution strategy that combined spatial and potential resolution, hybridization and antibody recognition, and ECL luminescence and quenching. The biosensor was fabricated by separately immobilizing two ECL reagents (gold nanoparticles/g-C3N4 nanosheets and ruthenium bipyridine derivative/gold nanoparticles/Nafion) and the capture DNA probe on the two sections of glassy carbon electrode. As a proof of concept, m6A-Let-7a-5p and m6A-miR-17-5p were chosen as model analytes, while m6A antibody-DNA3/ferrocene-DNA4/ferrocene-DNA5 was designed as an m6A-binding probe and DNA6/DNA7 was designed as a hybridization probe with DNA3 to release the quenching probes ferrocene-DNA4/ferrocene-DNA5. The recognition process led to the quenching of the ECL signals from both probes via BINSD. The proposed biosensor has the advantage of being washing-free. The ECL methods using the fabricated ECL biosensor with the designed probes exhibited a low detection limit of 0.03 pM for two m6A-RNAs and high selectivity. This work reveals that this strategy is promising for developing an ECL method for the simultaneous detection of two m6A-RNAs. The proposed strategy could be expanded to develop the analytical methods for the simultaneous detection of other RNA modifications by changing the antibody and hybridization probe sequences.
研究领域:
分析化学
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生物传感器
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癌症研究
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电化学
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纳米技术
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RNA生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）