Small
·
2025
·
IF 12.1
Qing Li,Ruyin Zhang,Chengpei Ouyang,Shuo Wang,Shanshan Li,Xinchao Yin,Zimeng Deng,Baoqin Han,Jinhua Chi
Corneal transplantation remains the prevailing treatment for corneal defects, which is always restricted by donor shortages and numerous postoperative complications accompanying suturing. Photocurable hydrogels have emerged as alternative therapeutic strategies for the repair of corneal defects, but most hydrogels focus on repairing focal corneal defects and still suffer from low transparency and poor mechanical properties. Herein, photocurable hydrogel GelMA/OCS composed of gelatin methacryloyl (GelMA) and oxidized chondroitin sulfate (OCS) is developed for sutureless repair of large corneal defects (6 mm). This injectable hybridized hydrogel demonstrates excellent transparency, low swelling rate, enhanced mechanical properties, and superior adhesion properties. In vitro experiments reveal that GelMA/OCS hydrogel can support the proliferation and migration, and adhesion growth of human corneal epithelial cells (HCECs), demonstrating satisfactory cytocompatibility and cell affinity. In addition, GelMA/OCS hydrogel is capable of accurately filling the large corneal defects in rabbits and forming hydrogel grafts with smooth surfaces. Postoperative slit lamp, histological evaluation, and transcriptomic analysis reveal that GelMA/OCS hydrogel can significantly facilitate corneal re-epithelialization and the integration and reconstruction of stromal structures, as well as reduce inflammation responses and scar formation. Therefore, GelMA/OCS hydrogel may provide a promising alternative for the sutureless treatment of large corneal defects.
研究领域:
生物材料学
·
眼科医学
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2022
·Vol. 9
·期 17
·2106115
·
IF 17.521
Qiuwen Zhu,Yi Hong,Yuxuan Huang,Yi Zhang,Chang Xie,Renjie Liang,Chenglin Li,Tao Zhang,Hongwei Wu,Jinchun Ye,Xianzhu Zhang,Shufang Zhang,Xiaohui Zou,Hongwei Ouyang
Adhesive patches are advanced but challenging alternatives to suture, especially in treating fragile internal organs. So far there is no suture-free adhesive patch based on metabolizable poly(amino acid) materials with excellent mechanical strength as well as immunomodulation functionality. Here, a polyglutamic acid-based elastic and tough adhesive patch modified by photosensitive groups on the surface to achieve robust light-activated adhesion and sealing of flexible internal organs is explored. With the porous internal morphology and excellent biodegradability, the patches promote regeneration through a macrophage-regulating microenvironment. Treated rabbits achieve rapid full-thickness gastric regeneration with complete functional structure within 14 d, suggesting its robust tissue adhesion and repair-promoting ability.
研究领域:
生物材料学
·
组织工程
·
免疫调节
·
再生医学
·
生物医学工程
·
消化系统外科
✓ 已验证
DOI
高影响因子
BIOMATERIALS
·
2024
·
IF 12.8
Long Zhao,Zhen Shi,Xia Qi,Jingting Wang,Mengmeng Yu,Muchen Dong,Fuyan Wang,Qingjun Zhou,Ting Wang,Weiyun Shi
A critical shortage of donor corneas exists worldwide. Hydrogel patches with a biological architecture and functions that simulate those of native corneas have garnered considerable attention. This study introduces a stromal structure replicating corneal patch (SRCP) composed of a decellularized cornea-templated nanotubular skeleton, recombinant human collagen, and methacrylated gelatin, exhibiting a similar ultrastructure and transmittance (above 80%) to natural cornea. The SRCP is superior to the conventional recombinant human collagen patch in terms of biomechanical properties and resistance to enzymatic degradation. Additionally, SRCP promotes corneal epithelial and stromal cell migration while preventing the trans-differentiation of stromal cells into myofibroblasts. When applied to an ocular surface (37 °C), SRCP releases methacrylated gelatin, which robustly binds SRCP to the corneal stroma after activation by 405 nm light. Compared to gelatin-based photocurable hydrogel, the SRCP better supports the restoration of normal corneal curvature and withstands deformation under an elevated intraocular pressure (100 mmHg). In an in vivo deep anterior-corneal defect model, SRCP facilitated epithelial healing and vision recovery within 2 weeks, maintained graft structural stability, and inhibited stromal scarring at 4 weeks post-operation. The ideal performance of the SRCP makes it a promising humanized corneal equivalent for sutureless clinical applications.
研究领域:
眼科
·
组织工程
·
生物材料
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）