JOURNAL OF FOOD SCIENCE
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2023
·Vol. 88
·期 12
·5291-5308
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IF 3.9
Yani Pan,Helin Lv,Fuyuan Zhang,Shuxi Chen,Yan Cheng,Shicheng Ma,Hao Hu,Xiyu Liu,Xiaoyong Cai,Fangyuan Fan,Shuying Gong,Ping Chen,Qiang Chu
Oral cavity contains the second largest microbial community in the human body. Due to the highly vascularized feature of mouth, oral microbes could directly access the bloodstream and affect the host healthy systemically. The imbalance of oral microbiota is closely related to various oral and systemic diseases. Green tea extracts (GTE) mainly contain tea polyphenols, alkaloids, amino acid, flavones, and so on, which equipped with excellent anti-inflammatory activities. Previous studies have demonstrated the beneficial effects of GTE on oral health. However, most researches used in vitro models or focused on limited microorganisms. In this study, the regulatory effect of GTE on oral microbiome and the alleviative effect on oral inflammation in vivo were evaluated. The results showed that GTE could efficiently alleviate the inflammations of the tongue, cheek pouch, as well as throat. GTE effectively inhibited the activation of NF-κB through the upregulation of the anti-inflammatory cytokine interleukin (IL)-10, consequently leading to reduced expression of pro-inflammatory cytokines IL-6 and tumor necrosis factor-α. The indexes of spleen and thymus were also elevated by GTE in stomatitis mice. Moreover, GTE promoted the growth of probiotics Lactobacillus and Bacillus , inhibited the reproduction of pathogens Achromobacter , reversing the microbiota disorders in oral cavity. This study not only presents a novel approach for enhancing oral microecology but also facilitates the wider adoption of tea consumption.
研究领域:
药理学
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微生物学
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免疫学
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口腔健康
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Xiuli Chen,Yanchu Ke,Ying Zhu,Mingbang Xu,Chao Chen,Shuguang Xie
Tetracycline (TC) is an antibiotic that is recently found as an emerging pollutant with low biodegradability . Biodegradation shows great potential for TC dissipation. In this study, two TC-degrading microbial consortia (named SL and SI) were respectively enriched from activated sludge and soil. Bacterial diversity decreased in these finally enriched consortia compared with the original microbiota. Moreover, most ARGs quantified during the acclimation process became less abundant in the finally enriched microbial consortia. Microbial compositions of the two consortia as revealed by 16 S rRNA sequencing were similar to some extent, and the dominant genera Pseudomonas , Sphingobacterium, and Achromobacter were identified as the potential TC degraders. In addition, consortia SL and SI were capable of biodegrading TC (initial 50 mg/L) by 82.92% and 86.83% within 7 days, respectively. They could retain high degradation capabilities under a wide pH range (4−10) and at moderate/high temperatures (25–40 °C). Peptone with concentrations of 4–10 g/L could serve as a desirable primary growth substrate for consortia to remove TC through co-metabolism. A total of 16 possible intermediates including a novel biodegradation product TP245 were detected during TC degradation . Peroxidase genes, tet X-like genes and the enriched genes related to aromatic compound degradation as revealed by metagenomic sequencing were likely responsible for TC biodegradation.
研究领域:
环境微生物学
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生物修复
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分子生态学
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宏基因组学
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产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 3.978
Tao Xu,Teng Liu,Dawei Jiang,Ziyue Yuan,Xiaoqiang Jia
Persistent organic pollutants (POPs) mainly including polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and organochlorine pesticides (OPs), have long-term harmful effects on human health and the environment. In this study, a natural consortium BP-W was attained from oil-contaminated soil using biphenyl as the sole carbon source, which is a relatively simple PAH and can form PCBs under certain catalytic conditions. PCR-DGGE techniques were used to analyze the structure of the consortium BP-W. The results showed that this consortium was composed of Chryseobacterium BP-1, Pseudomonas putida BP-2, Stenotrophomonas BP-3, Achromobacter BP-4, and an uncultured bacterium. The consortium BP-W degraded 99.88 % of 1 g L −1 biphenyl in 72 h. The degradation ratios of 100 mg L −1 naphthalene, phenanthrene, pyrene, and PCB77 in 5 days were 100 %, 97.54 %, 42.26 %, and 26.73 %, respectively. Notably, the consortium BP-W exhibited a high tolerance for these POPs. In the binary-substrate and ternary-substrate systems, the consortium BP-W was still able to rapidly degrade organic substrates. The results of gas chromatography-mass spectrometry showed that biphenyl was completely degraded by BP-W and entered the TCA cycle through the protocatechuic acid pathway, and no other highly toxic metabolites were produced during the degradation process. Thus, this consortium BP-W may have potential utility for the environmental-friendly and cost-effective bioremediation of POPs.
研究领域:
环境科学
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微生物学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
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