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PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY · 2024 · IF 2.8

Ficus carica Linn leaves extract induces cucumber resistance to Podosphaera xanthii by inhibiting conidia and regulating enzyme activity

Xin Li,Yuan Gao,Naveed Ahmad,Fanqi Bu,Mengfei Tian,Kaitao Jia,Wenxue Sun,Chunying Li,Chunjian Zhao

Cucumbers ( Cucumis sativus L.) are highly susceptible to infection by powdery mildew (PM), resulting in a deterioration of cucumber quality. The utilization of chemical control measures can effectively control cucumber PM; however, it may potentially give rise to issues such as environmental pollution, compromised safety, and drug resistance. The urgent need for a green, secure, and efficient strategy to manage cucumber PM necessitates attention. The predominant fungal pathogen causing PM in cucumbers is Podosphaera xanthii ( P. xanthii ) in this study. The preventive and control efficacy of Ficus carica Linn leaf extract (FLE) against P. xanthii was investigated for the first time. The preventive experiments entail pre-infection spraying of FLE prior to the occurrence of P. xanthii , while the curative experiments involve post-outbreak spraying of FLE. After comprehensive consideration, the optimal approach was determined to be spraying the front of cucumber leaves with a concentration of 0.04 mg/mL FLE in order to effectively control P. xanthii in leaf disc bioassay. The lowest superposition rate from FLE and trisodium phosphate dodecahydrate was 12.86 %, indicating that the impact of FLE on the respiratory metabolism of P. xanthii conidia primarily operates through the hexose monophosphate pathway (HMP). The growth of P. xanthii conidia and mycelium can be inhibited or delayed by FLE. The application of FLE can effectively mitigated chlorophyll loss, suppressed the activities of peroxidase (POD) and polyphenol oxidase (PPO), as well as enhanced the activities of phenylalanine ammonia lyase (PAL), chitinase , and β- 1,3-glucanase. Furthermore, three representative enzyme genes, PAL, POD, and chitinase , were chosen for the purpose of validation. The results suggested that FLE has the ability to modulate the expression of relevant enzyme genes, with peak effects observed after 3–4 days of treatment. The findings offer theoretical support for the advancement of novel botanical pesticides aimed at controlling crop diseases.

研究领域: 植物病理学 · 农业科学
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JOURNAL OF LUMINESCENCE · 2023 · IF 3.6

Green preparation of silanized carbon dots with ficus virens leaves as a potent antibacterial agent and an effective fluorescent sensor of iron ion

Shuchen Pei,Xiang Huang,Lin Lai,Wanlin Sun,Shuiqin Chai,Jun Chen

In this work, a green and economical strategy to prepare silanized carbon dots (Si-CDs) based on Ficus virens leaves was carried out. The prepared difunctional Si-CDs showed high water-solubility and favorable photostability. The morphology and surface functional groups were analyzed by TEM, FT-IR, and XPS. Si-CDs had amorphous nature with no crystal lattice and the average diameter of 1.95 nm. Some functional groups like nitrogen groups , oxygen groups, and silicon groups were discovered. Attributing to the small size of Si-CDs, positive charges, and the generation of singlet oxygen and hydrogen radicals, Si-CDs possessed effective antibacterial activity . The minimum inhibitory concentration of Si-CDs against Escherichia coli and Staphylococcus aureus was 0.078 mg/mL. Moreover, Si-CDs exhibited selective and sensitive fluorescence quenching response to Fe 3+ through static quenching process. The limit of detection was 0.84 μmol/L in the liner range of 5–50 μmol/L. Analysis of real samples led to 99.8–102.0% recoveries and <1% relative standard deviation. Consequently, this work discovered a new, green, economical silanized carbon dot with the potential application as a potent antibacterial agent and an effective fluorescent sensor of iron ion .

研究领域: 材料科学 · 生物学 · 环境科学
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE · 2024 · IF 3.3

Deep eutectic solvent (DES)-assisted extraction of pectin from Ficus carica Linn. peel: optimization, partial structure characterization, functional and antioxidant activities

Hongzhi Liu,Jihui Lin,Yaowu Hu,Hui Lei,Qian Zhang,Xiaoyu Tao,Dan Zhang,Hong Niu

BACKGROUND The pectin from Ficus carica Linn. (fig) peels is a valuable and recyclable constituent that may bring huge economic benefits. To maximize the utilization of this resource, deep eutectic solvent (DES)-assisted extraction was applied to extract pectin from fig peels, and the extraction process was optimized with response surface methodology. RESULTS When DES (choline chloride/oxalic acid = 1:1) content was 168.1 g kg −1 , extraction temperature was 79.8 °C, liquid–solid ratio was 23.3 mL g −1 , and extraction time was 120 min, the maximum yield of 239.6 g kg −1 was obtained, which was almost twice the extraction of hot water. DES-extracted fig peel pectin (D-FP) exhibited better nature than hot water-extracted fig peel pectin (W-FP) in terms of uronic acid content, particle size distribution, and solubility, but lower molecular weight and esterification degree. D-FP and W-FP had similar infrared spectra and thermodynamic peaks but differed in monosaccharide compositions. D-FP also showed good antioxidant capacities and exhibited better functional activities than W-FP. CONCLUSION These results indicated that D-FP was of promising quality being utilized in food or medical industries and the optimal DES-assisted extraction method might be applied as a sustainable process for the effective extraction of bioactive pectin from fig peels with the excellence of low equipment requirements and simple operation. © 2024 Society of Chemical Industry.

研究领域: 食品科学 · 生物化学 · 植物资源利用
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Antioxidants · 2024 ·Vol. 13 ·期 9 ·1084 · IF 6

Extraction, Characterization, and In Vitro Biological Activity of Polyphenols from Discarded Young Fig Fruits Based on Deep Eutectic Solvents

Qinqiu Zhang,Yue Peng,Yi Xu,Fan Li,Shuxiang Liu,Danka Bukvicki,Qing Zhang,Shang Lin,Miaomiao Wang,Tianyi Zhang,Dingtao Wu,Wen Qin

(1) Background: Discarded young fig fruits (DYFFs) result in a waste of resources, such as sparse fruits and residual fruits, and there has been no research on the relationship between phenolic compounds and biological activity in DYFFs (2) Methods: Different deep eutectic solvents (DESs) and 80% ethanol were used to prepare DYFF extracts, and polyphenol extraction efficiency and bioactivities in the DYFFs extracts were compared. (3) Results: More than 1700 phytochemicals were identified in DYFFs, and thirteen of these typical phenolic compounds were analyzed quantitatively; chlorogenic acid, rutin, luteolin 8-C-glucoside, and epicatechin are the main polyphenols in DYFFs, especially chlorogenic acid with 2720–7980 mg/kg. Ferulic acid, caffeic acid, epicatechin, (+)-catechin, luteolin 8-C-glucoside, rutin, hesperetin, and chlorogenic acid showed different degrees of correlation with in vitro antioxidant activity. Moreover, the highest total phenol content found in the extracts of ChCl-Ethylene glycol (Choline chloride:Ethylene glycol = 1:2) was 8.88 mg GAE/g DW, and all quantitatively analyzed phenolic compounds had high levels in various DESs and 80% ethanol. The 80% ethanol and Choline chloride (ChCl) solvent system showed the greatest antioxidant properties, and the Choline chloride-Urea (Choline chloride: Urea = 1:2) extract of DYFFs exhibited the highest inhibitory activity. (4) Conclusions: DESs have demonstrated potential as promising green solvents, especially the ChCl solvent system, which facilitates the extraction of polyphenols.

研究领域: 植物化学 · 食品科学 · 生物活性研究
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SCIENCE OF THE TOTAL ENVIRONMENT · 2019 · IF 5.589

Adsorption properties and mechanisms of novel biomaterials from banyan aerial roots via simple modification for ciprofloxacin removal

Huimin Fan,Yongwen Ma,Jinquan Wan,Yan Wang,Zhen Li,Yun Chen

The study investigated ciprofloxacin (CIP) adsorption capacity of the novel biomaterials prepared from banyan aerial roots by simple thermochemical modification. Pretreated banyan aerial root fibers were modified with a green reagent citric acid (1 M) at 90, 120 and 150 °C, which enhanced the fiber adsorption capacity revealed by characterization and adsorption tests. Several characterization methods were applied to exploring the surface morphologies and physicochemical properties of unmodified banyan aerial roots (UBARs) and modified banyan aerial roots (T-MBARs, T stands for the modification temperature). Based on SEM images and N 2 adsorption/desorption isotherms, the modification resulted in decrease of the specific surface area owing to cellulose molecular linking. In that case, the improved CIP adsorption of MBARs might be attributed to the larger carboxyl quantity and stronger electronegativity manifested via FTIR spectra and zeta potential analysis. Through the adsorption experiments, the optimal pH value of 8 and the suitable absorbent dosage of 0.03 g were obtained. The simulation results showed that the Freundlich model can fit the adsorption thermodynamic data quite well, while the kinetic data was simulated preferably by the pseudo-second-order kinetic equation signifying the chemical adsorption process, and the intra-particle diffusion was involved in the adsorption consisted of three stages. The findings of batch experiments under diverse operations represented that MBARs purified aqueous CIP better than UBARs, closely related to the superior electronegativity. Both characterization and adsorption studies illustrated the dominant role of electrostatic interaction during CIP removal, accompanied by hydrogen bonding and diffusion interaction besides. The present work suggested that MBAR fibers could possess a promising application to ciprofloxacin potent removal from aqueous solutions.

研究领域: 环境科学 · 材料科学
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INDUSTRIAL CROPS AND PRODUCTS · 2015 · IF 2.837

Enhancement of flavonoids extraction from fig leaf using steam explosion

Lanzhi Qin,Hongzhang Chen

Effects of plants’ structures on bioactive compounds extraction were rarely reported, which limited the research improvement of extraction efficiency from an intrinsic perspective. In this work, the structural properties of untreated and steam exploded fig leaves as well as their effects on flavonoids extraction were systematically studied. Results implied that internal diffusion became the limiting factor of flavonoids extraction from untreated fig leaf when liquid–solid ratio exceeded 75   mL/g-dry. After steam explosion pretreatment (0.2   MPa and 3   min), flavonoids yield extracted from fig leaf was increased by 55.9% compared with that from untreated fig leaf. However, more drastic steam explosion condition (0.4   MPa and 3   min) did not lead to higher flavonoids yield, which may be caused by the damage of flavonoids at high pretreatment temperature. A finite reaction model was proposed to demonstrate the mechanisms of flavonoids extraction from steam exploded fig leaf, and it was well consistent with the practical extraction. Therefore, steam explosion pretreatment with proper intensity should be a novel technology to enhance the extraction performance.

CAS / MDL: 153-18-4
研究领域: 植物学 · 食品科学 · 生物工程
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FOOD CHEMISTRY · 2023 · IF 8.8

A surface multiple imprinting layers membrane with well-oriented recognition sites for selective separation of chlorogenic acid from Ficus carica L.

Zidan Luo,Mengfei Tian,Naveed Ahmad,Yuxin Xie,Chunguo Xu,Jie Liu,Chunjian Zhao,Chunying Li

Chlorogenic acid (CGA), known as an important natural antioxidative compound in Ficus carica L, has valuable application prospects on health food, functional food, nutrition and dietary formulations. In this study, a surface multiple imprinting layers membrane (SMILM) was developed and applied to separate CGA from F. carica . Two different imprinting layers were integrated onto the membrane surface in sequence. The first imprinting layer was formed by dopamine polymerization and the second imprinting layer was fabricated by atom transfer radical polymerization (ATRP) and boronate affinity. The prepared SMILM with well-oriented multiple recognition sites exhibited high adsorption capacity (52.08 mg·g −1 in 60 min) for CGA and specific selectivity with imprinting factor ( IF ) of 3.06. Furthermore, the recognition mechanism of SMILM was clarified by molecular simulation and NMR. The SMILM was successfully applied to separate CGA from the fruits, peels and leaves of F. carica with recoveries of 90.22, 83.31 and 84.95 %, respectively.

研究领域: 植物化学 · 分离技术 · 材料科学
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MATERIALS RESEARCH BULLETIN · 2019 · IF 3.355

Photocatalytic potential of bio-engineered copper nanoparticles synthesized from Ficus carica extract for the degradation of toxic organic dye from waste water: Growth mechanism and study of parameter affecting the degradation performance

Muhammad Usman,Adeel Ahmed,Bing Yu,Qiaohong Peng,Youqing Shen,Hailin Cong

A facile and useful approach to the removal of possible health and environmental hazardous organic dye Alizarin Yellow R was completed by utilizing copper nanoparticles synthesized from Ficus Carica fruit extract. The polyphenols, proteins, and biomolecules present in the fruit extract were involved in the bio-reduction of Copper nanoparticles. The photocatalytic activity of the Copper nanoparticles was examined by the degradation of toxic organic dyes such as Alizarin Yellow R. These results displayed that photocatalytic capacity of Copper nanoparticles towards Alizarin Yellow R dye was more significant and maximum degradation performance of Alizarin Yellow R was 89.71%, respectively. Different parameters that affect the degradation performance were also studied to determine the ideal conditions for maximum photocatalytic efficiency. The chemical kinetics and possible photocatalytic degradation mechanism were also described. Due to the high degradation performance and stability, it could be possible that copper nanoparticles may have some beneficial effects in organic dyes removing from different industries wastewater in the future.

CAS / MDL: 2243-76-7
研究领域: 环境科学 · 材料科学 · 生物工程
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Journal of Industrial Textiles · 2019 · IF 1.884

Structure and properties of high quality natural cellulose nano fibrils from a novel material Ficus natalensis barkcloth

Amjad Farooq,Shuai Jiang,Aamir Farooq,Muhammad A Naeem,Afzal Ahmad,Lifang Liu

We report for the first time the extraction of cellulose nano fibrils from Ficus natalensis barkcloth fibers by means of chemical treatments and catalytic oxidation of cellulose fibers by using 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO). After every stage of treatments, the structural properties of barkcloth fibers powder (BC-PW), cellulose fibers (BC-NB) and cellulose nano fibrils (BC-CNF) were carefully characterized by means of scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for morphological analysis, Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction techniques for chemical analysis, and thermal properties were done by thermo-gravimetric analysis. FTIR results revealed the progressive removal of non-cellulosic contents and X-ray diffraction (XRD) analysis showed that the crystallinity increased with the successive alkali and bleaching treatments. Finally, by evaluating the TEM images, the average diameter of the nano-cellulose fibrils from Ficus natalensis barkcloth was also confirmed as 28 ± 0.6 nm and the length in hundred nano meters was recorded. The resultant cellulose nano fibrils maintaining the cellulose I structure had dimensional properties in nano-scale, higher crystallinity (68.5), and better thermal stability (305.62°C). The barkcloth cellulose nano fibrils can be used in nano technology like food packing material, nano composites and medical textiles.

研究领域: 材料科学 · 生物学
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Journal of Cleaner Production · 2017 · IF 5.715

Simultaneous extraction, transformation and purification of psoralen from fig leaves using pH-dependent ionic liquid solvent based aqueous two-phase system

Tong Wang,Cheng-Bo Gu,Sui-Xin Wang,Ping Kou,Jiao Jiao,Yu-Jie Fu

Recently, integrated and sustainable methods for extraction, transformation and purification of value-added compounds have gained increasing attention. In the present study, ionic liquid (IL)-acid based pH-dependent aqueous two-phase system (ATPS) was developed for simultaneous transformation psoralic acid-glucoside into psoralen, extraction and purification of psoralen from fig leaves for the first time. The cleaner [Bmim]Br-citric acid mixture showed enhanced transformation and extraction efficiency. The extraction yield of psoralen by [Bmim]Br-citric acid mixture was 1.45, 2.45 and 3.68 times higher than that of by [Bmim]Br-water, ethanol-critic acid and ethanol, respectively. The cleaner [Bmim]Br-citric acid based ultrasound extraction and transformation process was optimized using response surface method (RSM). Under optimum conditions, the maximum extraction yield of psoralen was 31.22 mg/g. Subsequently, we investigated and optimized the pH-dependent aqueous two-phase process. Under optimum conditions, the maximum recovery yield of psoralen was 96.34%. These results indicated that our method was efficient and had great potential for producing psoralen from fig leaves in large scale. What's more, this method could also been used to produce value-added compounds from other raw materials.

研究领域: 植物化学 · 药物化学
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JOURNAL OF POLYMERS AND THE ENVIRONMENT · 2021 ·Vol. 30 ·期 4 ·1547-1559 · IF 3.667

Novel Pretreatment Performance Evaluation for Cellulose Nanofibrils Extraction from Ficus natalensis Barkcloth

Farooq Amjad,Li Mengmeng,Alasood Abeer,Farooq Aamir,Ashraf Muhammad,Patoary Mohammed Kayes,Liu Lifang

Recently, nanosized cellulose materials extraction is extensively interesting from the sources of sustainable materials. Cellulose nanofibrils (CNF) extraction through green bio-based materials featured as promising interest in the field of science. In this study, dimethyl sulfoxide (DMSO) was applied to examine its effectiveness in pretreating the Ficus natalensis barkcloth cellulose (FNBC) for CNF production before 2,2,6,6,-tetramethylpiperidine-1-oxyl (TEMPO) oxidation. The pretreatment performance of DMSO was evaluated based on the structural and morphological changes. DMSO pretreated FNBC attained the most dramatic morphological changes as compared to untreated cellulose samples. The results of the scanning electron microscope (SEM) and transmission electron microscope (TEM) shows that there is an extensive structural disruption of FNBC during the pretreatment process, which could be because of outstanding ability to eliminate non-cellulosic materials and amorphous regions from the FNBC, confirmed by the X-ray diffractometry (XRD) showing higher crystallinity values, as well as higher thermal stabilities values of pretreated FNBC samples, were also noted. Overall, this study revealed a tremendously effective and pioneer pretreatment method for fractionating FNBC, to stimulate the successive extraction of cellulose nanofibrils. Furthermore, based on the cellulose and CNF characterizations, this study showed that F. natalensis barkcloth could be considered as an alternative source of cellulose for potential value-added industrial applications such as the food industry, paper making, and biomedicines. Graphic Abstract

CAS / MDL: 67-68-5 2564-83-2
研究领域: 材料科学 · 生物学
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FOOD HYDROCOLLOIDS · 2023 · IF 10.7

Digestion under saliva, simulated gastric and small intestinal conditions and fermentation in vitro by human gut microbiota of polysaccharides from Ficus carica Linn.

Bing Xu,Shiqing Song,Lingyun Yao,Huatian Wang,Min Sun,Haining Zhuang,Xiaowei Zhang,Qian Liu,Chuang Yu,Tao Feng

Previous studies have shown that Ficus carica polysaccharide (FCPS) extracted from common fig fruit exhibits antioxidant and immune-enhancing properties. Nevertheless, it remains uncertain whether FCPS can modulate the metabolism of gut microbiota. This study aims to assess whether the polysaccharides from two different types of figs can be broken down by the upper digestive system, including saliva, simulated stomach, and small intestinal conditions, and whether the gut microbiota can utilize them (Mw = 4.59 × 10 4 (LFCP, Low molecular weight), Mw = 6.08 × 10 4 (HFCP, High molecular weight)). The results revealed that FCPS retained its molecular weight and reducing sugar content even after passing through the upper digestive system, indicating its safe arrival in the gut without undergoing decomposition. In vitro FCPS fermentation of gut microbiota lowered the pH and produced more gas in the order of LFCPHFCP, respectively, indicating that it can be broken down and utilized by the microbiota. Additionally, the amount of short-chain fatty acids increased notably (LFCP < HFCP), indicating the higher the molecular weight, the stronger the acid production capacity. FCPS demonstrated the ability to significantly decrease the Firmicutes to Bacteroidetes ratio, potentially inhibiting obesity and inflammation. Therefore, FCPS is a promising functional food with potential to enhance health and prevent disease by promoting gut health.

CAS / MDL: 64-19-7 107-92-6 79-09-4
研究领域: 食品科学 · 微生物学 · 营养学
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