JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2013
·Vol. 160
·期 8
·B102
·
IF 2.588
Qian Han,Yonghua Wang,Yihan Huang,Liju Guo,Yingzi Fu
In the present study, a novel approach was designed to construct a reagentless chiral biosensor for highly selective recognition of N-isobutyryl-cysteine enantiomers. The reduced-graphene oxide sheets (rGO) and thionine (TH) were firstly stepwisely synthesized onto modified glassy carbon electrode using glutaraldehyde as a bifunctional reagent, and γ-globulin was adsorbed on the electrode surface as a chiral selector. TH-rGO with excellent redox electrochemical activities brought in more redox probes for chiral recogniton, which could improve the sensitivity of the biosensor. Good reproducibility and sufficient stability were obtained with the advantages of TH-rGO and glutaraldehyde reagent. Moreover, the proposed biosensor showed an obviously decreased current peak after adsorption of N-isobutyryl-L-cysteine isomer. As compared with penicillamine or cysteine enantiomers, the current biosensor showed good enantioselectivity for sensing N-isobutyryl-cysteine enantiomers.
研究领域:
分析化学
·
生物传感技术
·
电化学
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 3
·889-896
·
IF 4.58
Sun Xiaotian,Hui Ni,Luo Xiliang
A reagentless and label-free voltammetric modified electrode was developed for the carcinoembryonic antigen (CEA), an important biomarker for colorectal adenocarcinoma. It is based on the use of a reticular hybrid composite consisting of polyaniline (PANI) nanowires grown on the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) that was doped with an ionic liquid (IL). The composite features excellent electrical conductivity and a porous structure, a high specific surface and inherent redox activity. It was placed on a glassy carbon electrode (GCE) and antibody against CEA was immobilized on its surface. The redox current of PANI, measured typically at 0.16 V (vs. Ag/AgCl) serves as the analytical information. This electrode displays linear range that extends from 0.001 to 10 ng mL −1 , with a detection limit as low as 0.7 pg mL −1 . It also possesses excellent temporal stability and selectivity.
A sensitive, label-free and reagentless modified electrode for CEA was developed based on the combination of two different conducting polymers, polyaniline nanowires and porous PEDOT/IL nanocomposite.
研究领域:
电化学
·
生物传感器
·
癌症诊断
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Mei Wang,Hanfeng Cui,Nian Hong,Qingxia Shu,Xinru Wang,Yuping Hu,Guobing Wei,Hao Fan,Jing Zhang
In this paper, we first synthesized a novel tetraferrocene signal marker. Then a reagentless triplex DNA junctions-based electrochemical DNA sensor using signal amplification strategy of CHA and tetraferrocene was constructed. Tetraferrocene labeled hairpin probes (H1 and H2) were integrated together to form triplex DNA junctions through connecting chain (C1). It enabled catalytic hairpin assembly (CHA) reaction to be initiated without the addition of exogenous probe during the detection process, and the conformational change of the target caused the change of electrochemical signal. Combined with tetraferrocene and CHA strategies, the sensor achieved double signal amplification with a detection limit as low as 0.085pM. In addition, the sensor was successfully applied to mRNA detection in cells with high sensitivity and reagentless operation, providing possibilities for in vivo testing.
研究领域:
生物化学
·
分子生物学
·
分析化学
·
生物技术
✓ 已验证
DOI
高影响因子
Journal of Water Process Engineering
·
2025
·
IF 6.7
Xujie Lan,Xinrui Liu,Xia Qin,Honghyun Ren,Xingwei Tao,Cuicui Xu,Fanbin Zhang,Xiyang Li
The heterogeneous electro-Fenton (EF) process is a promising advanced-oxidation technology for treating recalcitrant landfill leachate nanofiltration concentrate (LNC), yet single-cathode or homogeneous EF systems remain limited by iron-sludge generation, narrow pH windows and sub-optimal coupling between H 2 O 2 production and activation. Here we engineer a dual-cathode heterogeneous EF (DEF) reactor that spatially decouples (i) high-rate in situ H 2 O 2 electrosynthesis on a carbon-black modified Ni-foam gas-diffusion cathode, from (ii) efficient copper-activated Fenton-like decomposition of H 2 O 2 on a Cu-coated Ni-foam cathode. The synergistic, reagent-free configuration operates from pH 3–7 without external Fe 2+ dosing and produces negligible metal sludge. Under the optimum current (0.718 A) and pH 3.5 determined by response-surface methodology, the DEF removed 72.74 % COD and 65.31 % TOC from real LNC within 120 min at an energy demand of 128 kWh/kg TOC—54 % lower than anodic oxidation—and maintained >90 % activity after five reuse cycles with ∼0.715 mg/ Cu leaching. Electron paramagnetic resonance (EPR) spectroscopy verified ·OH and ·O 2 − as the dominant oxidant, generated via a surface Cu 0 /Cu + /Cu 2+ redox cycle that accelerates H 2 O 2 decomposition. Compared with homogeneous EF and single-cathode heterogeneous EF benchmarks, the DEF offers comparable mineralization yet superior energy efficiency and zero-sludge operation, providing a scalable strategy for treating refractory industrial effluents.
研究领域:
环境工程
·
电化学
·
水处理技术
·
高级氧化工艺
·
工业废水处理
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Jing Zhang,Luoxing Yang,Jie Pei,Yanzhang Tian,Jiyang Liu
Convenient and sensitive detection of tumors marked in serum samples is of great significance for the early diagnosis of cancers. Facile fabrication of reagentless electrochemical immunosensor with efficient sensing interface and high sensitivity is still a challenge. Herein, an electrochemical immunosensor was easily fabricated based on the easy fabrication of immunoassay interface with electron transfer wires, confined redox probes, and conveniently immobilized antibodies, which can achieve sensitive and reagentless determination of the tumor marker, carcinoembryonic antigen (CEA). Carboxyl multi-walled carbon nanotubes (MWCNTs) were firstly modified with an electrochemical redox probe, methylene blue (MB), which has redox potentials distinguished from those of redox molecules commonly existing in biological samples (for example, ascorbic acid and uric acid). After the as-prepared MB-modified MWCNT (MWCNT-MB) was coated on the supporting glassy carbon electrode (GCE), the MWCNT-MB/GCE exhibited improved active area and electron transfer property. Polydopamine (PDA) was then in situ synthesized through simple self-polymerization of dopamine, which acts as the bio-linker to covalently immobilize the anti-CEA antibody (Ab). The developed immunosensor could be applied for electrochemical detection of CEA based on the decrease in the redox signal of MB after specific binding of CEA and immobilized Ab. The fabricated immunosensor can achieve sensitive determination of CEA ranging from 10 pg/ml to 100 ng/ml with a limit of detection (LOD) of 0.6 pg/ml. Determination of CEA in human serum samples was also realized with high accuracy.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
癌症诊断
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）