Chemosensors
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2022
·Vol. 10
·期 2
·83
·
IF 4.229
Wenqing Shao,Jiayu Mai,Zhenbo Wei
In this study, a novel cobalt polyphthalocyanine/carboxylic acid functionalized multiwalled carbon nanotube nanocomposite (CoPPc/MWCNTs-COOH) to detect lactic acid was successfully fabricated. The nanocomposite was systematically characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, ultraviolet–visible absorption spectroscopy, and X-ray photoelectron spectroscopy. The nanocomposite provided excellent conductivity for effective charge transfer and avoided the agglomeration of MWCNTs-COOH. The electrochemical surface area, diffusion coefficient and electron transfer resistance of the CoPPc/MWCNTs-COOH glassy carbon electrode (CoPPc/MWCNTs-COOH/GCE) were calculated asA= 0.49 cm2,D= 9.22 × 10−5cm2/s, andRct= 200 Ω, respectively. The lactic acid sensing performance of the CoPPc/MWCNTs-COOH was evaluated using cyclic voltammetry in 0.1 M PBS (pH 4). The results demonstrated that the novel electrode exhibited excellent electrochemical performance toward lactic acid reduction over a wide concentration range (10 to 240 μM), with a low detection limit (2 μM (S/N = 3)), and a reasonable selectivity against various interferents (ascorbic acid, uric acid, dopamine, sodium chloride, glucose, and hydrogen peroxide). Additionally, the electrode was also successfully applied to quantify lactic acid in rice wine samples, showing great promise for rapid monitoring applications.Keywords:nanocomposites;lactic acid;cobalt phthalocyanine;carbon nanotube;nonenzyme
研究领域:
生物化学
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材料科学
·
传感器技术
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xiu Qin,Chunling Yuan,Guoxing Geng,Rui Shi,Shiqi Cheng,Yilin Wang
An enzyme-free colorimetric method for sensitive detection of uric acid (UA) was established, which was achieved by inhibiting the etching of gold nanorods (AuNRs). The longitudinal direction of AuNRs would be gradually etched by I 2 , as an oxidant, generated by the reaction between MnO 2 nanosheets (MnO 2 NS) and I − . Benefitted from the length change, the localized surface plasmon resonance (LSPR) peak of AuNRs showed an obvious blue shift, led to the color change of AuNRs solution. Once UA was introduced, MnO 2 NS was reduced to Mn 2+ rapidly. As a result, the formation of I 2 was reduced and the etching of AuNRs was inhibited. Therefore, a simple method for the assay of UA was achieved with UV–vis absorption spectra and bare eyes, respectively. In optimization conditions, LSPR absorption peak shift (Δλ) of AuNRs before and after inhibition enabled the sensitive detection of UA in the range of 0.8−30 μM and 30−300 μM, the detection limit of low concentration range was 0.76 μM. The developed method has been utilized for determining UA in clinical samples with satisfactory results.
研究领域:
分析化学
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医学诊断
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纳米技术
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DOI
高影响因子
SENSORS
·
2018
·Vol. 18
·期 10
·3281
·
IF 2.475
Jingjing Zhang,Chunzheng Yang,Chaoqun Niu,Chen Liu,Xuepin Cai,Jie Du,Yong Chen
In this study, a label-free fluorescent, enzyme-free, simple, highly sensitive AND logic gate aptasensor was developed for the detection of adenosine triphosphate (ATP). Double-stranded deoxyribonucleic acid (DNA) with cohesive ends was attached to graphene oxide (GO) to form an aptasensor probe. ATP and single-stranded DNA were used as input signals. Fluorescence intensity of PicoGreen dye was used as an output signal. The biosensor-related performances, including the logic gate construction, reaction time, linearity, sensitivity, and specificity, were investigated and the results showed that an AND logic gate was successfully constructed. The ATP detection range was found to be 20 to 400 nM (R2= 0.9943) with limit of detection (LOD) of 142.6 pM, and the sensitivity range was 1.846 × 106to 2.988 × 106M−1. This method for the detection of ATP has the characteristics of being simple, low cost, and highly sensitive.Keywords:ATP detection;label-free fluorescence;enzyme-free;graphene oxide;logic gate
研究领域:
生物化学
·
分子生物学
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DOI
PubMed
高影响因子
Micromachines
·
2022
·Vol. 13
·期 11
·1902
·
IF 3.523
Yuke Han,Xiaomeng Bian,Misheng Liang,Tianshu Li,Lianqing Zhu,Xiaoguang Zhao,Rui You
An enzyme-free terahertz uric acid sensor based on a metallic slot array metamaterial was proposed and realized both theoretically and experimentally. The sensing model was verified in simulation and femtosecond laser processing technology was employed to ablate slots in the copper plate to fabricate metamaterials. Analytes were tested with liquid phase deposition on the metamaterial by a terahertz frequency domain spectroscopy system. Gradient concentrations of uric acid, ascorbic acid, and a mixture of them were measured separately with a good linear response. A significant decrease in sensitivity was observed in the ascorbic acid assay compared with the uric acid assay. The test results of the mixture also proved that our device is resistant to ascorbic acid. It is a simple and effective method for monitoring uric acid concentrations and the strategy of eliminating interference while modulating the resonance peak location mentioned here can be rationally projected for the development of other sensors.Keywords:uric acid detection;terahertz metamaterial;biosensor;enzyme-free detection
研究领域:
生物医学工程
·
传感技术
·
光学
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DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 15
·5834
·
IF 4.6
Kaili Wang,Shuang He,Bowen Zhang,Zhen Cao,Tingting Zhou,Jia He,Ganghui Chu
The development of non-enzymatic and highly active electrocatalysts for glucose oxidation with excellent durability for blood glucose sensors has aroused widespread concern. In this work, we report a fast, simple, and low-cost NaBH4reduction method for preparing ultrafine ternary PtPdCu alloy nanowires (NWs) with a 3D network nanostructure. The PtPdCu NWs catalyst presents significant efficiency for glucose oxidation-reduction (GOR), reaching an oxidative peak-specific activity of 0.69 mA/cm2, 2.6 times that of the Pt/C catalyst (0.27 mA/cm2). Further reaction mechanism investigations show that the NWs have better conductivity and smaller electron transfer resistance. Density functional theory (DFT) calculations reveal that the alloying effect of PtPdCu could effectively enhance the adsorption energy of glucose and reduce the activation energy of GOR. The obtained NWs also show excellent stability over 3600 s through a chronoamperometry test. These self-supported ultrafine PtPdCu NWs with 3D networks provide a new functional material for building blood glucose sensors and direct glucose fuel cells.Keywords:ultrafine PtPdCu nanowire;enzyme-free glucose oxidation;density functional theory
研究领域:
材料科学
·
电化学
·
生物传感器
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-10
·
IF 5.3
Feng Lei,Chen Junji,Yang Min,Wang Juan,Yin Shougen,Zhang Deliang,Qin Wenjing,Song Jibin
Ti 3 C 2 T x MXene/Cu x O composites were prepared by acid etching combined with electrochemical technique. The abundant active sites on the surface of MXene greatly increase the loading of Cu x O nanoparticles, and the synergistic effect between the different components of the composite can accelerate the oxidation reaction of glucose. The results indicate that at the working potential of 0.55 V (vs. Ag/AgCl), the glucose sensor based on Ti 3 C 2 T x MXene/Cu x O composite presents large linear concentration ranges from 1 µM to 4.655 mM (sensitivity of 361 µA mM −1 cm −2 ) and from 5.155 mM to 16.155 mM (sensitivity of 133 µA mM −1 cm −2 ). The limit of detection is 0.065 µM. In addition, the sensor effectively avoids the oxidative interference of common interfering species such as ascorbic acid, dopamine and uric acid. The sensor has good reproducibility, stability and acceptable recoveries for the detection of glucose in human sweat sample (97.5-103.3%) with RSD values less than 4%. Based on these excellent properties it has great potential for the detection of glucose in real samples. Graphical abstract
研究领域:
材料科学
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生物传感
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电化学分析
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Zhenting Zhao,Yongjiao Sun,Yueyun Huang,Jiarui Jin,Xiaohui Wei,Weiping Gong,Jie Hu,Yong Chen
An ultrasensitive electrochemical glucose sensor was developed by hydrothermal growth of cobalt copper carbonate hydroxide nanowires (CCCH NWs) on copper foam (CF) and surface in situ growth of zeolitic imidazolate framework-67 (ZIF-67) derived cobalt oxide (Co 3 O 4 ) (Co 3 O 4 @CCCH NWs/CF). Benefitting from the large accessible surface area, the heterojunction between Co 3 O 4 and CCCH NWs, as well as the high electrical conductivity of copper foam, the electrochemical properties of Co 3 O 4 @CCCH NWs/CF sensor were significantly improved. As demonstration, we achieved an ultra-high sensitivity of 16.01 μA/μM/cm 2 , a wide linear range of 0.001 ~ 2.0 mM, and remarkable fast response-recovery times (1.5 s and 1.3 s) for emzymeless glucose detection based on the optimized sensor. Moreover, the fabricated Co 3 O 4 @CCCH NWs/CF sensor has a good reproducibility, a long-term stability and anti-bending property for glucose detection. More interestingly, this sensor can be used to determine the glucose level of real serum samples, thereby providing a useful tool for enzymeless glucose monitoring.
研究领域:
材料科学
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生物医学工程
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 30
·7921-7929
·
IF 3.307
Huang Mujia,He Daiping,Wang Mingzhu,Jiang Ping
High electrical conductivity and more active sites exposure are crucial for improving the performance of electrocatalyst. Binary metal oxide nanoarray grown on conductive substrate offers a 3D self-supported electrode with a great promise in boosting its performance in enzyme-free glucose sensing. Here, NiMoO 4 nanosheet arrays anchored on carbon cloth (NiMoO 4 NSA/CC) was prepared via a simple hydrothermal synthesis and used as 3D self-supported electrode for enzyme-free glucose sensing. The morphology and composition of NiMoO 4 nanosheet have been characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The electrochemical results show that NiMoO 4 NSA/CC exhibits remarkable high catalytic activity towards glucose oxidation, with a wide linear response ranging from 1 μM to 0.9 mM, a high sensitivity of 4.13 mA/mM·cm 2 , and a low detection limit of 1 μM (S/ N = 3). The enhanced performance might be attributed to the merits of nanosheet arrays with large surface area, self-supported electrode with 3D open network, as well as bimetallic component with high conductivity. Furthermore, NiMoO 4 NSA/CC also shows good selectivity and reliability for glucose detection in human serum. This work offers a new pathway for the construction of enzyme-free glucose sensor with high performance.
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研究领域:
材料科学
·
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Fengyu Xie,Tingting Liu,Lisi Xie,Xuping Sun,Yonglan Luo
It is significantly important to design enzyme-free electrocatalyst for sensitive and selective detection of glucose (Glu). In this paper, we report that metallic nickel nitride nanosheet on Ti mesh (Ni 3 N NS/Ti) behaves as an efficient three-dimensional catalyst electrode for Glu electro-oxidation under alkaline conditions. We further demonstrate the use of such Ni 3 N NS/Ti as a non-enzymatic electrochemical Glu sensor superior in analytical performances. The introduction of the Ni 3 N NS/Ti can provide a high surface area and lead to high sensitivity to Glu. This sensor shows a fast amperometric response toward Glu and can achieve steady state current density within 5 s, a detection range of 0.2 μM–1.5 mM, a detection limit of 0.06 μM (S/N = 3), a response sensitivity of 7688 μA mM −1 cm −2 , and satisfactory selectivity and reproducibility. In addition, it also works effectively for Glu detection in human blood serum, promising its application toward determination of Glu in real samples.
研究领域:
分析化学
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生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Peng Liu,Xin Li,Xuechao Xu,Xiangheng Niu,Mengzhu Wang,Hengjia Zhu,Jianming Pan
Detection of pesticide residues is important because it is related to food and environmental safety. Although lots of sensors have been developed and applied for pesticide analysis, most of them rely on the use of vulnerable bioenzymes, weakening the highlights of these sensors. Here we recommend a bioenzyme-free colorimetric assay of malathion, a typical organophosphorus pesticide, based on the analyte inducing the oxidase-mimicking activity loss of Ag 3 PO 4 /UiO-66. Compared to counterparts, the Ag 3 PO 4 /UiO-66 composite can provide enhanced oxidase-mimetic catalytic activity to trigger the 3,3′-tetramethylbenzidine chromogenic reaction with the participation of dissolved O 2 . When malathion exists, it rapidly adsorbs onto the composite surface via their unique interactions and significantly shields the oxidase-like activity of Ag 3 PO 4 /UiO-66, thus affecting the catalytic chromogenic reaction. With such a principle, sensing of malathion in a wide linear range (0.0083–5.333 μg/mL) was realized with a detection limit 7.5 ng/mL. Given no biological elements were involved in the proposed assay, it exhibited robust and reliable performance in determining malathion in practical samples. Furthermore, a chromogenic hydrogel-based smartphone sensing system was developed for instrument-free on-site analysis of malathion. With good analytical performance and facile operation, our assay and device will find promising applications in phosphothionate pesticide determination.
研究领域:
分析化学
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环境科学
·
食品安全
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DOI
高影响因子
Materials
·
2021
·Vol. 14
·期 17
·5067
·
IF 3.623
Jiaxin Tang,Luo Wei,Shuaijie He,Jihui Li,Ding Nan,Liqiang Ma,Wanci Shen,Feiyu Kang,Ruitao Lv,Zhenghong Huang
An ultrasensitive enzyme-free glucose sensor was facilely prepared by electrodepositing three-dimensional dendritic Cu on a room temperature exfoliated graphite-derived film (RTEG-F). An excellent electrocatalytic performance was demonstrated for glucose by using Cu/RTEG-F as an electrode. In terms of the high conductivity of RTEG-F and the good catalytic activity of the dendritic Cu structures, the sensor demonstrates high sensitivities of 23.237 mA/mM/cm2, R2= 0.990, and 10.098 mA/mM/cm2, R2= 0.999, corresponding to the concentration of glucose ranging from 0.025 mM to 1.0 mM and 1.0 mM to 2.7 mM, respectively, and the detection limit is 0.68 μM. In addition, the Cu/RTEG-F electrode demonstrates excellent anti-interference to interfering species and a high stability. Our work provides a new idea for the preparation of high-performance electrochemical enzyme-free glucose sensor.Keywords:exfoliated graphite-derived film;dendritic Cu structures;enzyme-free glucose sensor
研究领域:
生物医学工程
·
材料科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Feng Gong,Yixia Yang,Xiaoyun Shan,Zhiyou Tan,Jingjing Qian,Songbai Tian,Xinghu Ji,Zhike He
Digital analysis is an effective single-molecule detection method and has attracted extensive attention in the field of bioassays. However, most digital assays require microchambers for signal compartmentalization. Herein, we developed a microchamber-free and enzyme-free digital assay by labeling paramagnetic beads directly with ultrabright fluorescent microspheres . In this assay, a DNA sandwich analysis was firstly performed on the bead to label a fluorescent microsphere. Then, the beads were loaded on the glass slide to form a monolayer film for signal readout. The whole analysis process does not require the participation of enzymes and the preparation of microchambers, which greatly simplifies the experimental steps and saves the costs. Furthermore, by introducing non-enzymatic hybridization chain reaction (HCR) and biotinylated DNA-conjugated gold nanoparticles (Au NPs-Bio), the capture efficiency and analytical sensitivity were improved. As a proof of concept , single-stranded DNA (ssDNA) of SARS-CoV-2 fragment was chosen as a model, and a detection limit of 1.5 fM was achieved. Spiked and recovery experiments on human serum and saliva samples validated the good performance of the proposed digital assay in real biological samples. The proposed assay provides a facile way of signal generation and readout for digital analysis.
研究领域:
生物技术
·
分子诊断
·
医学检验
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）