Marine Drugs
·
2019
·Vol. 17
·期 4
·195
·
IF 3.772
Ruowei Guan,Yuan Peng,Lutan Zhou,Wenqi Zheng,Xixi Liu,Pin Wang,Qingxia Yuan,Na Gao,Longyan Zhao,Jinhua Zhao
Apostichopus japonicusis one of the most economically important species in sea cucumber aquaculture in China. Fucosylated glycosaminoglycan fromA. japonicus(AjFG) has shown multiple pharmacological activities. However, results from studies on the structure of AjFG are still controversial. In this study, the deaminative depolymerization method that is glycosidic bond-selective was used to prepare the depolymerized products from AjFG (dAjFG), and then a series of purified oligosaccharide fragments such as tri-, hexa-, nona-, and dodecasaccharides were obtained from dAjFG by gel permeation chromatography. The 1D/2D NMR and ESI-MS spectrometry analyses showed that these oligosaccharides had the structural formula ofl-FucS-α1,3-d-GlcA-β1,3-{d-GalNAc4S6S-β1,4-[l-FucS-α1,3-]d-GlcA-β1,3-}n-d-anTal-diol4S6S(n= 0, 1, 2, 3; FucS represents Fuc2S4S, Fuc3S4S, or Fuc4S). Thus, the unambiguous structure of native AjFG can be rationally deduced: it had the backbone of {-4-d-GlcA-β1,3-d-GalNAc4S6S-β1-}n, which is similar to chondroitin sulfate E, and eachd-GlcA residue in the backbone was branched with al-FucS monosaccharide atO-3. Bioactivity assays confirmed that dAjFG and nonasaccharides and dodecasaccharides from AjFG had potent anticoagulant activity by intrinsic FXase inhibition while avoiding side effects such as FXII activation and platelet aggregation.Keywords:Apostichopus japonicus;fucosylated glycosaminoglycan;oligosaccharide;unambiguous structure;anti-Fxase
研究领域:
海洋生物学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Aquatic Food Product Technology
·
2017
·
IF 0.478
Jia-Run Han,Zhi-Mo Zhu,Hai-Tao Wu,Na Sun,Yue Tang,Cui-Ping Yu,Chen-Chen Zhao,Zheng-Yu Zhang,Ao-Ting Li,Jia-Nan Yan
Maillard reaction products (MRPs) were produced in sea cucumber gut hydrolysates (SCGHs)-ribose system at 75°C, 85°C, and 95°C with a pH value of 7.0 or 8.0 for 12 hour. The parameters of amount of intermediate products, fluorescence intensity, browning development, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability showed a positive correlation with each other in SCGHs-ribose system. The amount of intermediate products, fluorescence intensity, and browning development followed the zero-order kinetic with activation energy from 62 to 91 kJ/mol in the Maillard reaction of SCGHs-ribose system. Moreover, 2-methyl-butanal has positive and significant correlation with DPPH radical scavenging ability of SCGHs-ribose MRPs. These results suggest that Maillard reaction improves the antioxidant activity of SCGHs, which contributes to the generation of intermediate, fluorescent, browning, and volatile products of SCGHs-ribose system.
研究领域:
食品化学
·
营养科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2019
·Vol. 13
·期 4
·2790-2797
·
IF 1.415
Han Yi-Tong,Zhao Chen-Chen,Han Jia-Run,Yan Jia-Nan,Du Yi-Nan,Shang Wen-Hui,Wu Hai-Tao
The purpose of the present study was to explore the antioxidant activity of organic extracts from Maillard reaction products (MRPs) of sea cucumber ( Stichopus japonicus ) gut hydrolysates (SCGHs). The SCGHs were obtained after treatment with neutrase, and they were then reacted with ribose at a mass ratio of 1:2, pH 8.0, and 95 °C for up to 12 h. The MRPs were extracted by methanol and ethanol. The methanol extracts of the MRPs had a higher UV absorbance, browning intensity, and fluorescence intensity than the crude MRPs. The scavenging capacities of methanol and ethanol extracts from MRPs in both 2′,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assays increased significantly ( p < 0.05). The methanol extracts were better than the ethanol extracts showing the reduced IC 50 levels for ABTS and DPPH by 50% and 50%, respectively. The inhibitory activity for peroxidation of linoleic acid of MRPs was also enhanced in the methanol extracts of MRPs, but this was reduced in ethanol extracts. Moreover, HepG2 cell viability after exposure to the methanol extracts of MRPs was increased to 59.30 ± 0.43% compared with the SCGHs and MRPs, which was 3.97 times that of the H 2 O 2 -treated group. These results suggest that methanol extraction can improve the antioxidant activity of SCGHs-ribose MRPs and that the corresponding extracts can be potentially used as food antioxidants.
研究领域:
食品科学
·
营养学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）