JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Zheng Liu,Ruonan Jia,Feng Chen,Guilong Yan,Weiguo Tian,Jinming Zhang,Jun Zhang
Iron corrosion is a subject of great technological importance and extensive public concern. However, the highly efficient detection of iron corrosion at early stage is still a challenging task. Herein, bright fluorescent carbon dots (CDs) with superior response to Fe 3+ were prepared by simple solvothermal process based on citric acid and ammonia. The obtained CDs are able to rapidly, sensitively and selectively respond to Fe 3+ . The quantitative analysis showed that the CDs exhibited a linear response to Fe 3+ in the range of 10 to 300 µM, with a detection limit of 0.9 μM. And the fluorescence quenching of CDs was obvious enough to be detected by the naked eyes. Such promising responsiveness of CDs offers a great opportunity for real-time and visual detection of Fe 3+ during electrochemical corrosion process. In addition, due to the excellent stability and solubility of CDs, patterned papers and hydrogels have been fabricated utilizing cellulose and PVA as matrices. The as-prepared biocompatible, environmental-friendly and disposable CDs based fluorescent materials were successfully used for detecting the degree of iron corrosion. This could provide a simple and visual strategy for monitoring the safety of structural metal materials.
研究领域:
材料科学
·
电化学
·
分析化学
✓ 已验证
DOI
高影响因子
ANALYST
·
2018
·Vol. 144
·期 1
·284-289
·
IF 3.864
Lei Zhang,Xue Shan,Leilei Guo,Jikang Zhang,Junliang Ge,Qing Jiang,Xinghai Ning
Nitroreductase activities are positively associated with the hypoxic level of tumors, making it an attractive target for tumor detection. Herein, we have developed a 2,5-bis(methylsulfinyl)-1,4-diaminobenzene based probe (BBP), which is a nitroreductase (NTR) responsive fluorescent probe and can rapidly detect NTRs with high sensitivity and specificity. The BBP showed not only a selective response to NTRs over other biological reductants, but also high sensitivity to NTRs and could detect as low as 20 ng mL−1 NTRs. Furthermore, the BBP responded rapidly to NTRs in as fast as 10 minutes, enabling real-time monitoring of the production levels of NTRs. Most importantly, the BBP could identify NTR activities in 2D cell monolayers, 3D tumor spheroids, and even solid tumors in mice. Particularly, the BBP could monitor the early tumor formation and treatment response via measuring NTR activities. Overall, the BBP appears to be an ideal imaging probe for the detection of solid tumors, and possesses great potential in a broad range of diagnostic and therapeutic applications in the clinic.
研究领域:
肿瘤学
·
分子影像学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Theranostics
·
2016
·
IF 8.854
Zhengyang Jia,Lina Song,Fengchao Zang,Jiacheng Song,Wei Zhang,Changzhi Yan,Jun Xie,Zhanlong Ma,Ming Ma,Gaojun Teng,Ning Gu,Yu Zhang
Developing ultrasensitive contrast agents for the early detection of malignant tumors in liver is highly demanded. Constructing hepatic tumors specific targeting probes could provide more sensitive imaging information but still faces great challenges. Here we report a novel approach for the synthesis of ultra-small Fe 3 O 4 nanoparticles conjugated with c(RGDyK) and their applications as active-target T 1 -weighted magnetic resonance imaging (MRI) contrast agent (T 1 -Fe 3 O 4 ) for imaging tiny hepatic tumors in vivo . RGD-modified T 1 -Fe 3 O 4 nanoprobes exhibited high r 1 of 7.74 mM -1 s -1 and ultralow r 2 /r 1 of 2.8 at 3 T, reflecting their excellent T 1 contrast effect at clinically relevant magnetic field. High targeting specificity together with favorable biocompatibility and strong ability to resist against non-specific uptake were evaluated through in vitro studies. Owing to the outstanding properties of tumor angiogenesis targeting with little phagocytosis in liver parenchyma, hepatic tumor as small as 2.2 mm was successfully detected via the T 1 contrast enhancement of RGD-modified T 1 -Fe 3 O 4 . It is emphasized that this is the first report on active-target T 1 imaging of hepatic tumors, which could not only significantly improve diagnostic sensitivity, but also provide post therapeutic assessments for patients with liver cancer.
研究领域:
医学影像学
·
纳米医学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Chaoran Yu,Yao Shen,Ce Bian,Miao Liu,Michael D. Weir,Hockin H. K. Xu,Zhiyi Yao,Yuxing Bai,Ning Zhang
Enamel demineralization is a common complication of orthodontic treatment. Studies related to enamel demineralization are usually conducted in the laboratory using in vitro models. Detection technology relies on large instruments, which are not conducive to its popularization and promotion. We designed a fluorescence sensing system using a complex formed by 3,4,9,10-tetra-(4-trimethylaminohexyloxy-carbonyl)-perylene (PDI-HTMA) and casein phospho-peptides (CPPs) that can rapidly detect calcium ions in enamel. In this study, we investigated the feasibility of using PDI-HTMA/CPPs as probes for detecting enamel demineralization. We used Ca 2+ from enamel demineralization as the detection target for the fluorescent probe and verified its stability, specificity, and linear range. This method exhibits strong linear relationships in the Ca 2+ concentration range of 30–500 μM, with a limit of detection of 3.89 μM. The PDI-HTMA/CPPs were applied to human enamel specimens. We compared the detection results with those of existing enamel demineralization evaluation methods, and the results showed a trend consistent with that of ICDAS grading and laser fluorescence caries detection. This fluorescent probe assay is expected to be applied for the early detection of enamel demineralization, which has strong application advantages. To the best of our knowledge, this is the first fluorescent probe assay applied for the in vitro detection of enamel demineralization.
研究领域:
口腔医学
·
材料科学
·
生物化学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yang Xiang,Boying Zhang,Changgeng Zhang,He Zhu,Qi Zhang,Shiping Zhu
Adhesives are essential for binding materials and ensuring the integrity of structural systems. However, adhesive joints often experience force accumulation, leading to potential failure, which remains challenging to monitor in real-time. In this study, we introduce a simple, cost-effective, and versatile strategy for fabricating force-indicating smart adhesives (FISAs) using commercially available materials, including polyacrylate matrices and silicone microspheres as fillers. These adhesives exhibit a pronounced mechanochromic response, driven by reversible cavitation within the elastomer matrix, enabling both qualitative and quantitative visualization of stress distribution at adhesive joints. This capability allows for early detection of adhesive failure, offering a proactive warning mechanism. The adhesive properties, including force sensitivity, are highly tunable by adjusting the polyacrylate matrix composition, microsphere content, size, and interfacial strength, making these FISAs adaptable to a wide range of substrates. This work represents a significant advancement in smart adhesive technology, combining enhanced monitoring capabilities with broad applicability across diverse scenarios.
研究领域:
材料科学
·
力学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Pharmacology
·
2020
·
IF 4.225
Wang Xiao-Shuang,Sun Zhi,Liu Li-Wei,Du Qiu-Zheng,Liu Zhang-Suo,Yang Yan-Jie,Xue Peng,Zhao Hong-Yu
Background: Oral lichen planus (OLP) is a T-cell-mediated chronic inflammatory disorder and precancerous oral lesion with high incidence. The current diagnostic method of OLP is very limited and metabolomics may provide a new approach for quantitative evaluation.Methods: The Ultra-Performance Liquid Chromatography-Quadrupole/Orbitrap High Resolution Mass Spectrometry (UHPLC-Q-Orbitrap HRMS) was applied to analyze the change of metabolites in serum of patients with OLP. A total of 115 OLP patients and 124 healthy controls were assigned to either a training set (n = 160) or a test set (n = 79). The potential biomarkers and the change of serum metabolites were profiled and evaluated by multivariate analysis.Results: Totally, 23 differential metabolites were identified in the training set between OLP group and healthy group. Three prominent metabolites in receiver operating characteristic (ROC) were selected as a panel to distinguish OLP or healthy individuals in the test set, and the diagnostic accuracy was 86.1%.Conclusions: This study established a new method for the early detection of OLP by analyzing serum metabolomics using UHPLC-Q-Orbitrap HRMS, which will help in understanding the pathological processes of OLP and identifying precancerous lesions in oral cavity.
研究领域:
医学
·
代谢组学
·
病理学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULAR MEDICINE
·
2025
·
IF 6.4
Quanpeng Qiu,Yi Ding,Xiaolong Guo,Jing Han,Jiaqi Zhang,Yaping Liu,Junjun She,Yinnan Chen
Background Extrachromosomal circular DNA (eccDNA) has potential in tumor diagnosis, particularly for improving diagnostic accuracy and early cancer detection; however, many challenges remain in its application to clinical practice. Methods We conducted a Circle‐Seq analysis on clinical samples at different stages of colorectal cancer progression to examine the dynamic changes of eccDNA during the progression of colorectal cancer. We used breakpoint-specific PCR to verify candidate eccDNAs identified by Circle‐Seq. The results were further validated using the AOM/DSS-induced colorectal cancer model. Results There was an increase in the abundance of eccDNA with the progression of colorectal cancer. The genes associated with these eccDNA molecules were primarily related to signaling pathways involved in tumor development and metastasis. Our analysis also revealed that eccDNA abundance positively correlates with gene expression, and eccDNA derived from specific genes has potential value for the early diagnosis of tumors. Conclusions This study revealed a connection between eccDNA and colorectal cancer progression and highlights the clinical potential of eccDNA for the early diagnosis of colorectal cancer. Supplementary Information The online version contains supplementary material available at 10.1186/s10020-025-01164-y.
研究领域:
医学
·
分子生物学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2021
·
IF 5.161
Feifei Su,Xianchao Du,Ting Shen,Anjun Qin,Weihua Li
Hidden corrosion of metal materials can cause catastrophic failure of engineering structures without warning, especially in harsh ocean environments. Thus, convenient, sensitive and effective early detection of metal corrosion has a great significance on the safety control of engineering facilities. In this research, we developed a novel aggregation-induced emission luminogen (AIEgen), TPM (( E )- N -(1 H -indazol-3-yl)-1-(4-(1,2,2-triphenylvinyl)phenyl)menthanimine), applied in epoxy coating, in which the visualization of the early stages of metal corrosion have been realized through a fluorescent ‘Turn-On’ phenomenon. Excellent luminescence response is observed on this coating with only 0.5‰ TPM content. Visible fluorescent ‘Turn-On’ phenomenon can be triggered on the delaminated TPM epoxy coating after immersion in solution at pH = 4 for 5 h, which ensures the rapidity and convenience of corrosion detection. The fluorescent response mechanism and intramolecular charge transfer (ICT) effect are finally discussed by combining the theoretical calculation with the experimental results. This research is an infrequent work on the early detection of the metal corrosion based on aggregation-induced emission luminogens (AIEgens). Moreover, this coating exhibit better corrosion resistance (∼10 10 Ω cm 2 ) on Q235 than that on tinplate and 304 steels, and enhanced the toughness of the coating.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Chenyue Zhan,Jiatian Cheng,Bowen Li,Shuailing Huang,Fang Zeng,Shuizhu Wu
Melanoma is a type of highly malignant and metastatic skin cancer, and early detection of melanoma by analyzing the level of its biomarker may decrease the likelihood of mortality. In this study, a fluorescent probe called NBR-AP for detecting tyrosinase (a biomarker of melanoma) has been created by incorporating a hydroxyphenylurea group (as a substrate for the enzyme) onto a fluorescent dye phenoxazine derivative (as an activatable signal reporter). This probe can be activated to generate fluorescence through a tyrosinase-mediated oxidation followed by hydrolysis of the urea linkage. The probe is able to detect the endogenous tyrosinase level in live cells and in zebrafish sensitively and selectively. Moreover, by imaging the tyrosinase activity, NBR-AP has been successfully applied to diagnose the melanoma and its metastasis in xenogeneic mouse models established via subcutaneous injection of B16F10 cells.
研究领域:
癌症生物学
·
分子生物学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2024
·
IF 8.2
Kai Wang,Ke-Feng Gu,Jie Cao,Yu-Shun Yang,Hai-Liang Zhu,Jin-Hui Shang,Jie-Li Zhou
Copper ions, implicated in processes such as oxidative stress and inflammation, are believed to play a crucial role in cardiovascular disease, a prevalent and deadly disease. Despite this, current diagnostic methods fail to detect early stage cardiovascular disease or track copper ion accumulation, limiting our understanding of the disease’s progression. Therefore, the development of noninvasive techniques to image copper ions in cardiovascular disease is urgently needed to enhance diagnostic precision and therapeutic strategies. In this study, we report the successful synthesis and application of a copper ion-activated photoacoustic probe, CS-Cu, which exhibits high sensitivity and selectivity toward copper ions both in vitro and in vivo. CS-Cu was able to noninvasively monitor the changes in copper ion levels and differentiate between different mice based on copper ions in urine. Furthermore, the probe demonstrated good photoacoustic stability and exhibited no significant toxicity in the mice. These findings suggest that CS-Cu could be a promising tool for early detection and monitoring of Cu2+ levels in vivo and urine, providing a new perspective on the role of copper ions in cardiovascular disease.
研究领域:
生物医学工程
·
诊断成像
·
分子生物学
·
心脏病学
✓ 已验证
DOI
PubMed
高影响因子
Heliyon
·
2024
·
IF 3.4
Song Lin,Lixin Wen,Hong Zhao,Donghua Huang,Zuwei Yang,Qinge Zou,Ling Jiang
Since the p53 protein is an important promising biomarker of lung tumor and colorectal tumor, it is very essential to design a highly effective mean to monitor the degree of p53 for the early clinical analysis/therapy of the related tumors. In this work, a sandwich-type electrochemical immunosensing (SES) platform is proposed for the first time to detect p53 via synthesizing Ti 3 C 2 T x MXene nanoribbons (Ti 3 C 2 T x Nb) and ferrocene/gold nanoparticles (Fc/Au) respectively as the sensing substrate and signal-amplifier. The superior electrical property and large surface area of Ti 3 C 2 T x Nb are beneficial to assemble the initial p53-antibodies (Ab 1 ), while the synthesized Fc/Au is devoted to assemble the secondary p53 antibodies (Ab 2 ) and gives a magnified signal. By adopting the Fc molecules as the probes, the experiments reveal the response current of Fc resulted from the SES structure increases along with the p53 increase from 1.0 to 200.0 pg mL −1 . A considerable low detection limit (1.0 pg mL −1 ) is achieved after optimizing several key conditions, it is thus confirmed the as-proposed SES mean exhibits significant application in the detection of p53 protein and other targets.
研究领域:
生物医学工程
·
肿瘤学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2024
·
IF 8.2
Luyun Xu,Yuanmei Chen,Jianqing Ye,Min Fan,Guibin Weng,Yongshi Shen,Zhizhong Lin,Duo Lin,Yuanji Xu,Shangyuan Feng
Lung cancer has become the leading cause of cancer-related deaths globally. However, early detection of lung cancer remains challenging, resulting in poor outcomes for the patients. Herein, we developed an optical biosensor integrating surface-enhanced Raman spectroscopy (SERS) with a catalyzed hairpin assembly (CHA) to detect circular RNA (circRNA) associated with tumor formation and progression (circSATB2). The signals of the Raman reporter were considerably enhanced by generating abundant SERS “hot spots” with a core–shell nanoprobe and 2D SERS substrate with calibration capabilities. This approach enabled the sensitive (limit of detection: 0.766 fM) and reliable quantitative detection of the target circRNA. Further, we used the developed biosensor to detect the circRNA in human serum samples, revealing that patients with lung cancer had higher circRNA concentrations than healthy subjects. Moreover, we characterized the unique circRNA concentration profiles of the early stages (IA and IB) and subtypes (IA1, IA2, and IA3) of lung cancer. These results demonstrate the potential of the proposed optical sensing nanoplatform as a liquid biopsy and prognostic tool for the early screening of lung cancer.
研究领域:
医学诊断
·
纳米生物技术
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）