Biofabrication
·
2024
·Vol. 17
·期 1
·015017
·
IF 8.2
Xiaoling Cao,Yonghao Deng,Zhongye Xu,Tingting Wang,Bing Tang,Jiande Han,Rui Guo,Rong Yin
Emergency wounds are often accompanied by bacterial infection, oxidative stress, and excessive inflammation due to the inability to quickly close and stop bleeding, resulting in chronic wounds that are difficult to heal. Clinically, surgical suturing is the fastest method for wound closure, but it is only suitable for wounds with small bleeding volumes and causes unsightly scar formation. Consequently, there is a critical need for hemostatic dressings versatile enough to address a spectrum of diverse and intricate wounds, especially in emergency scenarios. In this study, we constructed a unique versatile natural gelatin-based hydrogel with hemostasis, antibacterial, and anti-inflammation properties. The hydrogel was composed of 4-(4-(hydroxymethyl)-2-methoxy-5-nitrophenoxy) butyrylethylenediamine-modified methacrylated gelatin (GelMA-NB) and epigallocatechin gallate-grafted polylysine (EPL-EGCG), which imparts adhesion, antibacterial and antioxidant properties to the hydrogel. Simultaneously, the hydrogel was loaded with GelMA microspheres encapsulating natural resveratrol (RES@GM). This combination not only exhibited outstanding hemostatic capabilities but also preserved the anti-inflammatory potential of RES. In different animal models, the hydrogel exhibited outstanding hemostatic and wound healing effects, down-regulated the expression of IL-1β to promote inflammatory regulation and potential for angiogenesis and anti-scar. In conclusion, unique versatile natural gelatin-based hydrogel suitable for various complex wounds provides a promising strategy for emergency wound dressing applications.
研究领域:
生物材料学
·
医学
·
药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2014
·Vol. 71
·期 3
·597-605
·
IF 1.547
Zhang Hui,Zhan Kan,Chen Yulu,Chen Guoxia,Zhou Xiaomei,Liu Junxiu,Wu Min,Ni Henmei
Three dimensional Liesegang spherical layers of CaHPO 4 in gelatin ball were performed by employing CaCl 2 and Na 2 HPO 4 as the inner and outer electrolyte, respectively. Effects of concentrations of inner and outer electrolyte as well as pH on the morphologies of Liesegang rings (LRs) were investigated. As a result, it was observed that the time law, spacing law and width law found in 1D/2D gel systems were obeyed in this 3D gelatin system. The interaction of Ca 2+ and HPO 4
2− with gelatin matrix played a key role to the formation of LRs due to the existence of carboxylic groups on the gelatin chains. Using Ca 2+ as the inner electrolyte, LRs were prepared. However, employing HPO 4
2− as inner electrolyte, LRs were not obtained. Moreover, pH of gelatin solution greatly impacted on the formation of LRs. The number of LRs increased with the decrease of pH, whereas the width inversely decreased. pH 4.40 was a turn point, from which the spacing coefficient abruptly increased as pH increased. All these results indicated that the network was created by the interaction of Ca 2+ and –COO − of gelatin chains, which dominated the formation of CaHPO 4 LRs in gelatin.
研究领域:
生物材料学
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物理化学
·
软物质物理
·
胶体科学
·
生物矿化
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DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Lijuan Xu,Rongqiang Chen,Tian Ren
Microbial spoilage is a major reason of global food waste. Photodynamic inactivation (PDI) showed promise as an antimicrobial strategy owing to the reactive oxygen species (ROS) produced by photosensitizer, but the short lifespan of ROS limited its penetration into cells and accordingly hindered its effectiveness against Gram-negative bacteria. In this study, a photodynamic composite film with aggregation-induced emission behavior and dual antimicrobial activity was developed by incorporating natural photosensitizer nanoparticles with ε-Polylysine (PL) into a matrix composed of gelatin and dialdehyde nanocellulose. The advantage of this design lay in the PL's role as a " forward team”, facilitating ROS penetration through perforating the external membrane or cell wall of bacteria, thereby enhancing the PDI efficiency and achieving a 6 log CFU/sample bacterial reduction within 15 min of light exposure. Moreover, the composite film exhibited minimal ultraviolet transmittance, high transparency (exceeding 86.57 %), hydrophobicity, and enhanced mechanical strength. Pilot studies further confirmed the composite film's effectiveness in inhibiting microbial growth, protein oxidation, lipid oxidation, and pH of pork samples, successfully extending the shelf life of fresh pork to 10 days at 4 °C. Therefore, this study proposes a green and efficient synergistic antimicrobial strategy with PDI, demonstrating a potential application for enhanced pork preservation.
研究领域:
食品科学
·
微生物学
·
材料科学
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DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Tianhao Ying,Chenghong Jiang,Sadia Munir,Ru Liu,Tao Yin,Juan You,Jianhua Rong,Shanbai Xiong,Yang Hu
This work aimed to synthesize oregano essential oil/β-cyclodextrin microcapsules (OEO/β-CDs) and then prepare gelatin-based controlled-release antibacterial films with different OEO/β-CDs contents (0%–2%) for chilling preservation of grass carp fillets. The results of FTIR , XRD, DSC and accelerated release ratio showed that OEO was successfully encapsulated in OEO/β-CDs and its thermal stability was effectively improved. Moreover, at 2% of addition amount of OEO/β-CDs, the tensile strength of the films increased from 14.43 MPa to 18.72 MPa. In addition, the films showed significant antibacterial activity against Pseudomonas (61.52%), Aeromonas (62.87%), and Shewanella putrefaciens (66.67%). Preservation experiments showed that the films effectively prevented the increase of TVB-N, and TBA value of the refrigerated fillets and significantly suppressed the growth of spoilage organisms , thus extending the shelf life by 2–3 days. Therefore, the synthesized film has promising potential as an active packaging material for the preservation of grass carp.
研究领域:
食品科学
·
生物技术
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Jingxian Jiang,Qinghua Zhang,Xiaoli Zhan,Fengqiu Chen
Designing multifunctional adsorbents that can satisfy sophisticated requirements in complex water treatment fields remains challenging these days. In this work, an original and environment-friendly gelatin-based organic-inorganic composite aerogel equipped with integrative functions was fabricated through facile methods. The uniform dispersion of TiO 2 nanoparticles was successfully achieved in the three-dimensional structure of the composite aerogel, and the branched polyethyleneimine (PEI) was cross-linked onto it through the effect of glutaraldehyde. The gelatin/TiO 2 /PEI (GTP) aerogel had a hierarchical porous structure and a superamphiphilic surface, which endowed it with excellent oil/water separation properties, for both oil/water free mixtures and oil-water emulsions. Both cationic (methylene blue and crystal violet) and anionic (congo red and methyl orange) organic dyes could be adsorbed by the GTP aerogel with good adsorption capacity. It was noteworthy that the GTP aerogel was capable of realizing dye degradation because of its photocatalytic performance. Furthermore, this composite aerogel was able to remove copper ions from aqueous solutions with high capacity and good reusability. The regeneration of the GTP aerogel was realized by methanol and EDTA for dyes and copper ions respectively. Combined with comprehensive functions including oil/water separation, organic dyes adsorption, copper ions removal and photocatalytic property, the bio-based organic-inorganic composite GTP aerogel holds great promise for practical usage in water remediation, especially in the treatment of polluted water with complex components.
研究领域:
材料科学
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环境科学
·
化学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Lujia Yang,Yichen Zhou,Jiaojiao Xu,Xinyi Ma,Jiayi Yuan,Bihe Yuan
The frequency occurrence of building fires necessitates response materials with high flame retardancy and temperature sensitivity. Herein, we synthesized a gelatin/poly(acrylamide- co -acrylic acid)/lithium bromide/sodium phytate/glycerol hydrogel (Gly-GAPL) using in situ radical polymerization and solvent exchange techniques. Gly-GAPL exhibits notable thermoelectric performance (the Seebeck coefficient: 8.66 mV/K), temperature sensitivity, commendable mechanical properties and flame retardancy. Remarkably, Gly-GAPL features a rapid response time, triggering an alarm within 2 s upon exposure to flame. Gly-GAPL is highly resistant to ignition and significantly enhances the fire resistance of wood coated with it. Furthermore, its high transparency, impressive water retention and adhesion further underscore its potential as a flame-retardant coating for various inflammable materials. Given its outstanding thermoelectric performance and temperature sensitivity, an early fire-warning system is rapidly activated, promptly sending alerts to smart devices. This work introduces a novel strategy for developing smart flame retardancy materials and advances the applications of ionic hydrogels in early fire-warning systems.
研究领域:
材料科学
·
火灾安全工程
·
高分子化学
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产品关联
DOI
PubMed
高影响因子
Food and Bioprocess Technology
·
2017
·Vol. 10
·期 9
·1727-1736
·
IF 2.576
Ge Liming,Zhu Mingjin,Xu Yongbin,Li Xinying,Li Defu,Mu Changdao
The utilization of petroleum-derived synthetic materials causes severe ecological problems, such as environmental pollution and soil degradation. Hence, using naturally derived and renewable materials to fabricate novel biodegradable films for safe and effective food packaging has been a subject of interest over the years. Here, the novel antimicrobial and controlled biodegradable gelatin-based edible films were developed using nisin as the antimicrobial agent, amino-functionalized montmorillonite as the nanoparticle filler, and dialdehyde xanthan gum as the crosslinking agent. The results indicate that the ultraviolet barrier ability, water resistance, and mechanical properties of gelatin-based edible films are obviously improved on account of the crosslinking effect. Moreover, the resulting films demonstrate antimicrobial activity against Staphylococcus aureus owning to the addition of nisin. Furthermore, the crosslinking can slow down the erosion of the edible films by fungi due to the improved hydrophobicity and compact structure. Particularly, the edible films can be completely biodegraded in soil within 30 days. Meanwhile, the rate of soil biodegradation can be well controlled by adjusting the crosslinking degree. Overall, this novel gelatin-based edible films have potential applications in food packaging.
研究领域:
食品科学
·
材料科学
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产品关联
DOI
高影响因子
Gels
·
2024
·Vol. 10
·期 5
·318
·
IF 5
Weijie Zhang,Bing Zhang,Yihu Wang,Xiaofeng Cao,Jianing Wang,Weipeng Lu,Yanchuan Guo
Hydrogels with adhesion properties and a wetted structure are promising alternatives to traditional wound dressing materials. The insufficiency of gelatin hydrogels in terms of their adhesive and mechanical strength limits their application in wound dressings. This work presents the design and preparation of a gelatin-based hydrogel functionalized with dopamine (DA) and layered double hydroxide (LDH). The combination of DA and LDH improves the hydrogel’s adhesion properties in terms of interfacial adhesion and inner cohesion. Hydrogels with 8% DA and 4% LDH attained the highest adhesion strength of 266.5 kPa, which increased to 295.5 and 343.3 kPa after hydrophobically modifying the gelatin with octanoyl and decanoyl aldehydes, respectively. The gelatin-based hydrogels also demonstrated a macroporous structure, excellent biocompatibility, and a good anti-inflammatory effect. The developed hydrogels accelerated wound healing in Sprague Dawley rat skin full-thickness wound models.
研究领域:
材料科学
·
生物材料
·
组织工程
·
伤口护理
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Zihang Yan,Sixing Lai,Suqi Yang,Kefan Ouyang,Yuanyuan Feng,Hao Hu,Xing Hu,Hua Xiong,Qiang Zhao
Active gelatin-based films (CUR-PLLF) incorporating V-type granular starch-curcumin complex (VGS-C) and ε-polylysine (PLL) were developed for the short-term refrigerated preservation of salmon fillets. The experiment demonstrates that the composite film (CUR-PLLF), exhibits an enhanced elongation at break by 36.67%, accompanied by a marked increase in DPPH radical scavenging activity from 2.40% to 9.07%. The water vapor permeability and oxygen permeability of CUR-PLLF film were reduced by 14.02% and 18.09%, respectively, and it can be completely degraded in soil within 14 d, demonstrating significant environmental advantages. In salmon fillets refrigeration experiments, CUR-PLLF inhibited bacterial proliferation and fat oxidation, resulting in a 17.25% reduction in Total volatile base nitrogen level, which were below the spoilage threshold of 25 mg/100 g. Additionally, the content of thiobarbituric acid-reactive substances decreased by 30.77%. These findings suggest that the developed gelatin-based film exhibits comprehensive advantages in antibacterial properties, antioxidant activity, and biodegradability, offering an efficient and environmentally friendly solution for the preservation of salmon during short-term refrigeration.
研究领域:
食品科学
·
生物材料
·
环境科学
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产品关联
DOI
高影响因子
STAR Protocols
·
2023
·
IF 0
Jingtan Zhu,Xiaomei Liu,Jianyi Xu,Zhang Liu,Yating Deng,Junyao Dai,Tingting Yu,Dan Zhu
Summary The majority of fluorescent vessel labeling techniques currently available are limited by their expense, incomplete labeling, or complexity. Here, we present VALID ( v essel l a beling via ge l atin-based l i pophilic d ye solution)—a protocol for complete labeling of different vascular networks. We describe steps for preparing different dye hydrogels, murine vascular casting and tissue harvesting, immunolabeling, tissue clearing, and imaging, as well as detailed analysis of the vascular networks. This protocol is helpful for evaluating vascular lesions in studying different vessel-associated diseases. For complete details on the use and execution of this protocol, please refer to Zhu et al. 1
研究领域:
生物学
·
医学成像
·
血管生物学
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DOI
PubMed
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jian Yang,Haojie Yu,Li Wang,Xiaowei Liu,Yudi Huang,Yichuan Hong,Shuning Ren
Infected wound management is a great challenge to healthcare, especially in emergencies such as accidents or battlefields. Hydrogels as wound dressings can replace or supplement traditional wound treatment strategies, such as bandages or sutures. It is significant to develop novel hydrogel-based wound dressings with simple operation, inexpensive, easy debridement, effective antibacterial, biocompatibility, etc. Here, we designed a novel gelatin-based hydrogel wound dressing Gel-TA-Fe 3+ . The hydrogels used tannic-modified gelatin as the main body and Fe 3+ as the crosslinking agent to achieve a controllable rapid sol-gel transition. The hydrogels exhibited tough mechanical properties, excellent antibacterial ability, biocompatibility and an acceptable temperature response to near-infrared light (NIR). Moreover, the hydrogels could promote the healing process of MRSA -infected skin wound in rats. This multifunctional hydrogel was thought to have potential for emergency treatment of bacterial infected wound.
研究领域:
材料科学
·
生物医学工程
·
伤口愈合
·
抗菌治疗
·
生物材料
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2019
·
IF 2.442
Qingxi Hu,Chuang Wu,Haiguang Zhang
Electrospinning is promising approach for producing biomimetic vascular scaffolds. In particular, gelatin-based electrospun scaffolds offer excellent biocompatibility. However, gelatin-based vascular scaffolds are difficult to remove from the collector after electrospinning and cross-linking agents such as glutaraldehyde, which is commonly used to improve the strength of water-soluble electrospun gelatin, are highly toxic. Herein, we present a novel electrospinning method for preparing three-layered vascular scaffolds. First, Tween 80 was added to 2,2,2-trifluoroethanol as the polymer electrospinning solution and the gelatin electrospinning solution was mixed with polylactic acid and polycaprolactone, respectively. The three-layered vascular scaffold was fabricated by electrospinning an inner layer of gelatin, a middle layer of polyester, and another outer layer of gelatin. Finally, the electrospun scaffolds were cross-linked with microbial transglutaminase and vacuum dried. Biomechanical properties of the scaffold were determined by tensile testing. Furthermore, the structure and biocompatibility of the scaffolds were assessed by hydrophilicity tests, scanning electron microscopy, and cell seeding experiments. Our results suggest the proposed electrospinning method is suitable for preparing biomimetic vascular scaffolds for cardiovascular tissue engineering applications. Moreover, this paper provides a useful reference for the preparation and optimization of vascular scaffolds.
研究领域:
组织工程学
·
生物材料科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
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