PHARMACEUTICAL CHEMISTRY JOURNAL
·
2020
·Vol. 54
·期 2
·154-161
·
IF 0.538
Ying-Jiao Liu,Li-Juan Xie,Lan Xiao,Li-Min Gong,Ta-Si Liu,Nai-Hong Chen
Cinnamomum camphora Chvar. Borneol (CCCB) and Cinnamomum camphora Chvar. Cineol (CCCC) are two different chemical types of C. camphora . The clinical application of these chemical types of CC shows big differences, but sometimes they are confused because of the similarity of morphological features. To identify the two types of CC and to analyze the differences of compositions, their morphological characteristics were investigated by microscopic techniques and the chemical components were determined by GC-MS analysis and GC internal standard method. Distinguishing features of morphological characteristics were the shape, petiol, gland, and smell. Microscopic study of the transverse sections of leaves showed 13 – 15 columns xylem vessel in the leaves of CCCB, 11 – 13 columns xylem vessel, and more obvious and thicker cell walls of fiber cells in the leaves of CCCC. From this research, GC-MS analysis of essential oil of the leaves could provide mainly differential characteristics to distinguish between CCCB and CCCC. The total of 33 compounds were identified in the essential oil of CCCB leaves and the main component was d -borneol (55.11%), while d -borneol in CCCC leaves was not the main component and the amount of d -borneol might be related to the microstructure of gland. L(-)-camphor and d-borneol were quantitatively analyzed by GC with FID detector, using methyl salicylate as the internal standard. Each gram of the total essential oil contains 0.68 g of d -borneol in CCCB, and each gram of the total essential oil contains 0.150 and 0.015 g of L(-)-camphor in CCCB and CCCC, respectively, from Hunan province. Other habitats showed the same trend. This research was aimed to establish a method based on the microscopic, GC-MS, and GC techniques to identify and quantify CCCB and CCCC compositions, for providing scientific data to ensure with accuracy the origin of CCCB and CCCC plant material.
研究领域:
植物学
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药物分析
·
中药学
✓ 已验证
DOI
高影响因子
Advanced Sensor and Energy Materials
·
2022
·
IF 0
Jia Gao,Rui-Yang Zhao,Yi-Guang Wang,Ruo-Chen Xie,Wei Wang
We present a fluorescent microscopic method using an ultra-pH-sensitive polymeric probe to rapidly map within subsecond the pH distribution resulting from oxygen reduction reaction electrocatalysed by an array of platinum nanoparticles. Upon voltammetry of the surface-supported Pt catalysts, fluorescent quenching waves are observed to depend on the electrode potential. The spatiotemporal fluorescent evolution is then confirmed under a constant potential control to be due to the local pH change as a function of diffusing time by an estimation of the proton diffusion coefficient ( L α t 1 / 2 ) . On these bases, the fluorescent measurements at short reaction times can provide quantitative information regarding the one and two dimensional pH distributions, which are shown to exhibit the expected shape of a typical diffusion-driven concentration gradient. Such imaging of proton/pH profiles may find important applications such as efficient screening of different micro/nanoscale electrocatalysts.
研究领域:
分析化学
·
电化学
·
材料科学
·
生物传感器
✓ 已验证
DOI
COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
·
2024
·
IF 8.1
Haozhen Li,Chong Wei,Zixiong Cao,Yi Zhang,Xiaoqiang Li
Monitoring microstructure evolution during the preparation has always been a difficult problem in the modification studies of SiC composite matrix. Here, we used X-ray tomography microscopy to observe the microstructure of SiC f /SiC-W-ZrB 2 composites at different fabrication stage. Based on deep learning, the tracking of the densification process of matrix-modified SiC f /SiC composites was achieved and its suitability for microstructure reconstruction was also verified. The results showed that the average errors of reconstructed SiC f /SiC, pore and Metal (W/ZrB 2 ) are respectively 7.53%, 8.31% and 0.96% by comparison with the segmentation results. Compared with the experimental results, the average error and the average relative error of reconstructed SiC f /SiC is less than 3% and 3.74%.
研究领域:
材料科学
·
计算机科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Yanzi Xu,Haoke Zhang,Ning Zhang,Xiaochi Wang,Dongfeng Dang,Xunan Jing,Duo Xi,Ying Hao,Ben Zhong Tang,Lingjie Meng
To achieve super-resolution imaging in biological research using stimulated emission depletion (STED) nanoscopy, organic luminescent materials and their corresponding fluorescent nanoparticles with high brightness and photostability are of great significance. Herein, donor-acceptor-typed DBTBT-4C8 bearing flexible alkyl chains was developed, not only to afford deep-red emission from 600 to 800 nm but also to obtain high fluorescent brightness with the absolute photoluminescence quantum yields of 25%. After that, well-defined and monodispersed spherical nanoparticles using DBTBT-4C8 with bright emission, excellent biocompatibility, and photostability, which can easily mix with amphipathic block polymers, were then produced for super-resolution in vitro and in vivo imaging using STED nanoscopy. The observations showed that in contrast to confocal microscopy with a full width at half-maximum (FWHM) value of ≈400 nm, superior resolution with a significantly improved FWHM value of only 100 nm was achieved in biomedical cell imaging, which was also used to reconstruct three-dimensional images of stained HeLa cells at an ultrahigh resolution. More importantly, by using the prepared fluorescent organic nanoparticles (FONPs) in STED nanoscopy, in vivo imaging in glass catfish with largely enhanced resolution was also successfully achieved, demonstrating that these developed deep-red FONPs here are highly suitable for super-resolution in vitro and in vivo imaging using STED nanoscopy.
研究领域:
生物研究
·
材料科学
·
纳米技术
·
成像技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Qiong Duan,Lin Lin,Yue Liu,Xinlong Tang,Liang Fang,Chunhua Lu,Zhongzi Xu
Inspired by tortoise beetle, a novel method for preparing sustainable films, leveraging the on-demand embedding or release behavior of the functional fillers to impart or eliminate functions to the film is reported. The microscopic shape-memory behavior of the film surface was characterized using microscopy techniques and finite-element analysis to investigate the embedding and release of single micro-/nanospheres on the film surface, which were affected by sphere diameters and programming temperatures. By utilizing different functional fillers and distribution densities, the transformation and tunability of film properties such as optical reflection and fluorescence was demonstrated. Different functions of the film were altered through multiple cycles, showcasing the ability to immobilize patterns, step-wise release, recycle materials and create temperature-sensitive anticounterfeiting codes and decoration on objects. This approach offers a promising avenue for developing sustainable films with tunable functions and highlights the potential for recycling functional fillers, contributing to both environmental sustainability and material reusability.
研究领域:
材料科学
·
可持续性
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纳米技术
·
表面工程
✓ 已验证
产品关联
DOI
高影响因子
Light-Science & Applications
·
2025
·Vol. 14
·期 1
·1-10
·
IF 23.4
Li Zhonghao,Cai Zhihan,Wang Yuhan,Liu Yuliang,Li Guifeng,Yang Xi,Deng Ming,Chen Yu-Cheng,Yang Jichun,Luo Yang,Gong Chaoyang,Zhu Tao
The mechanical properties of biological fluids serve as early indicators of disease, offering valuable insights into complex physiological and pathological processes. However, the existing technologies struggle to achieve high-throughput measurement, limiting their widespread applications in disease diagnosis. Here, we propose laser-emission vibrational microscopy of microdroplets for high-throughput measurement of the intrinsic mechanical properties of fluids. The microdroplet array supporting high Q-factor (10 4 ) whispering gallery modes (WGM) lasing was massively fabricated on a superhydrophobic surface with inkjet printing. Ultrasound was employed to actuate the mechanical vibrations of the microdroplets, and the vibration amplitude was quantified using time-resolved laser spectra. We found that the stimulus-response of the laser emission is strongly dependent on the liquid viscosity. Fast mapping of the microdroplets’ viscosities was achieved by stage scanning. High-throughput screening of hyperlipidemia disease was also demonstrated by performing over 2000 measurements within 25 min. Thanks to the small volume of the microdroplets, a single drop of blood can support over seven million measurements. The high-throughput ability and small sample consumption make it a promising tool for clinical diagnoses based on mechanical properties.
研究领域:
生物医学工程
·
光学与光子学
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微流控技术
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临床诊断
·
生物力学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULES
·
2017
·
IF 5.835
Haie Zhu,Min Chen,Shuxue Zhou,Limin Wu
A conventional optical microscope fails to resolve features with sizes smaller than 200 nm due to the Abbe diffraction limit. Herein, we synthesize two kinds of high refractive index and shape controllable colloidal polymer microspheres, poly(9,9′-bis[4-(2-acryloyloxyethyloxy)phenyl]fluorene) (poly(BAEPF)) and poly(2-phenylphenoxyethyl acrylate) (poly(OPPEA)), used for solid immersion lens. It is the high refractive index and close contact with the surface features that can make the colloidal polymer microsphere-based microlens effectively collect evanescent information on the substrate and form a clear and magnified virtual image at certain position below the substrate surface. Thus, as small as 60 nm feature size on the substrate can be clearly resolved through the microlens under a visible light optical microscope, which is far beyond the resolution limit of a visible light microscope.
研究领域:
材料科学
·
光学
·
显微技术
✓ 已验证
DOI
高影响因子
OPTICS EXPRESS
·
2025
·Vol. 33
·期 9
·20023-20036
·
IF 3.3
Yuhan Nie,Yuhan Nie,Jie Hu,Siying Zhang,Xiangzhou Meng,Xiangzhou Meng,Guo Fu,Guo Fu,Guo Fu
Single-molecule localization microscopy (SMLM) breaks the diffraction limit, significantly enhancing optical resolution and providing a powerful tool for imaging complex biological structures in life sciences. However, imaging biological structures below 10 nm and studying protein-protein interactions remains a challenge for optical super-resolution microscopy. Here, we introduce a microscopy based on two-color single-molecule localization microscopy, we call it molecular Imaging by two-color imaging (MITI), which improves resolution to the molecular level, reaching several nanometers. We demonstrate MITI is suitable for samples labeled with organic dyes and photoactivatable/photoswichable fluorescent proteins. MITI can resolve two dyes separated by ssDNA with 2-10 nm length as well as ffDronpa and PAmCherry1 fused to the N- and C-terminal of FtsZ (ffDronpa-FtsZ-PAmCherry1). MITI is versatile and easy to realize, and thus, it will expand the applications of molecular scale super-resolution microscopy in life sciences.
研究领域:
生物物理学
·
细胞生物学
·
显微技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELL
·
2025
·
IF 42.5
Zizhu Tan,Chia-Heng Hsiung,Jiahui Feng,Yangye Zhang,Yihan Wan,Junlin Chen,Ke Sun,Peilong Lu,Jianyang Zang,Wenxing Yang,Ya Gao,Jiabin Yin,Tong Zhu,Yang Lu,Zijian Pan,Yilong Zou,Can Liao,Xiaosong Li,Yuxuan Ye,Yu Liu,Xin Zhang
Fluorescence microscopy has been widely applied in the life sciences. While intensity as a steady-state signal is widely used, the time-resolved (tr) signal using fluorescence lifetime remains underexplored. Herein, we present a family of time-resolved fluorescent proteins (tr-FPs) with rationally controlled lifetimes. Using a strategy that regulates lifetime without affecting the spectra of FPs, we have developed a series of tr-FPs that cover the visible spectrum and a wide range of lifetimes. The tr-FPs are employed in temporal-spectral resolved microscopy, allowing for the simultaneous imaging of 9 different proteins in live cells and the correlation of multiple activities to cell cycles. Furthermore, tr-FPs enable multiplexing super-resolution microscopy that concurrently visualizes 4 proteins using the lifetime signal and are demonstrated to quantify the stoichiometry of cellular proteins. Our work introduces the concept and development of tr-FPs as a transformative toolset, presenting opportunities to integrate system complexity and quantitative accuracy into biological research.
研究领域:
分子生物学
·
生物物理学
·
细胞生物学
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光学成像
·
蛋白质工程
·
显微技术
·
系统生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Energy & Environmental Science
·
2024
·
IF 32.4
Xuedong Zhang,Ziang Guo,Xin Li,Qiunan Liu,Huan Hu,Fangyuan Li,Qiao Huang,Liqiang Zhang,Yongfu Tang,Jianyu Huang
Lithium electrochemistry dictates the safety and cycling durability of lithium metal batteries. Although there have been numerous spectroscopy studies of lithium in liquid electrolyte batteries, electron microscopy studies of lithium in solid state batteries are scarce due to the technical difficulty of sample fabrication. In this work, we introduce an innovative specimen preparation methodology dubbed cryo-ultramicrotomy to fabricate large-scale cryo-TEM specimens that permit direct visualization of global anode/electrolyte interfaces and lithium within the polymer electrolyte from the nanometer to millimeter scale. By combining electron energy loss spectroscopy and multivariate least squares fitting, we map the lithium/PEO interface and the deposited lithium clusters, revealing a novel tri-layer SEI|Li|SEI structure on the lithium anode surface, indicating that electrons can tunnel through the SEI layer. The deposited lithium features either isolated or interconnected clusters that are different from the lithium dendrites in liquid electrolytes. The lithium clusters comprise Li0 and LiH cores surrounded by inorganic components such as Li2O and LiOH but with little LiF, which are further enwrapped by the organic components. Based on this understanding, tetrabutylammonium fluoride was introduced into the electrolyte to tailor the SEI and PEO structures to significantly improve the electrochemical performance of lithium metal batteries.
研究领域:
材料科学
·
电化学
·
显微技术
✓ 已验证
DOI
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2024
·
IF 3.9
Jialian Ding,Bin Su
Graphical One plus one is greater than two: A remarkable electrochemiluminescence (ECL) enhancement of Ru(bpy) 3 2+ /TPrA is observed by introducing another coreactant, TEOA, with a 10.8-fold increase of ECL intensity and 1.7-fold extension of ECL layer. The enhancement was interpreted by a chemical oxidation route, in which TEOA + ⋅ acts as an enhancer to produce more TPrA + ⋅ and meanwhile allow the remote formation of TPrA + ⋅. This dual-coreactants strategy works not only in solution with freely diffusive Ru(bpy) 3 2+ but also on Ru(bpy) 3 2+ -functionalized microbeads, showing promise in ECL-based bioassay and microscopy. The electrochemiluminescence (ECL) of ruthenium(II) tris(2,2′-bipyridyl) (Ru(bpy) 3 2+ ) with tri- n -propylamine (TPrA) as the good coreactant can be unexpectedly enhanced by a weak coreactant, such as triethanolamine (TEOA). First, the intensity of ECL emitted by Ru(bpy) 3 2+ /TPrA can be remarkably amplified by 10.8-fold after adding some amount of TEOA. Moreover, the ECL layer thickness, measured by self-interference spectroscopy, is also doubled. The enhancement far exceeding the superposition of respective contribution of TPrA and TEOA was elucidated by a “chemical oxidation mechanism”, in which TEOA + ⋅ acts as a chemical enhancer to oxidize TPrA in solution and to accelerate significantly the ECL reaction kinetics. This mechanism was proved by single-photon counting experiment and finite element simulations. In addition, the dual-coreactants strategy works well not only in solution with freely diffusive Ru(bpy) 3 2+ , but also on Ru(bpy) 3 2+ -functionalized microbeads, suggesting that ethanolamines could act as cheap, easily available and low-background enhancers for ECL-based bioanalysis and microscopy.
研究领域:
分析化学
·
生物分析
·
显微技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NATURE
·
2024
·Vol. 630
·期 8017
·643-647
·
IF 50.5
Zhang Qiubo,Song Zhigang,Sun Xianhu,Liu Yang,Wan Jiawei,Betzler Sophia B.,Zheng Qi,Shangguan Junyi,Bustillo Karen C.,Ercius Peter,Narang Prineha,Huang Yu,Zheng Haimei
Electrified solid–liquid interfaces (ESLIs) play a key role in various electrochemical processes relevant to energy 1 , 2 , 3 , 4 , 5 , biology 6 and geochemistry 7 . The electron and mass transport at the electrified interfaces may result in structural modifications that markedly influence the reaction pathways. For example, electrocatalyst surface restructuring during reactions can substantially affect the catalysis mechanisms and reaction products 1 , 2 , 3 . Despite its importance, direct probing the atomic dynamics of solid–liquid interfaces under electric biasing is challenging owing to the nature of being buried in liquid electrolytes and the limited spatial resolution of current techniques for in situ imaging through liquids. Here, with our development of advanced polymer electrochemical liquid cells for transmission electron microscopy (TEM), we are able to directly monitor the atomic dynamics of ESLIs during copper (Cu)-catalysed CO 2 electroreduction reactions (CO 2 ERs). Our observation reveals a fluctuating liquid-like amorphous interphase. It undergoes reversible crystalline–amorphous structural transformations and flows along the electrified Cu surface, thus mediating the crystalline Cu surface restructuring and mass loss through the interphase layer. The combination of real-time observation and theoretical calculations unveils an amorphization-mediated restructuring mechanism resulting from charge-activated surface reactions with the electrolyte. Our results open many opportunities to explore the atomic dynamics and its impact in broad systems involving ESLIs by taking advantage of the in situ imaging capability.
研究领域:
材料科学
·
电化学
·
显微技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）