ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Ru-Jia Yu,Wei Ma,Mao-Pan Peng,Zhi-Shan Bai,Yi-Tao Long
A novel redox-mediated chromogenic reaction was demonstrated based on the reaction between HAuCl 4 and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), which generate various color responses from red to green in the resulting solutions. Various redox substance could be used to mediate the reaction and trigger a distinct color response. We established a sensitive hydrogen peroxide colorimetric sensor based on the redox-mediated chromogenic reaction and depicted the application both in detection of enzyme and in an immunoassay . Combining the traditional chromogenic reagent with gold nanoparticles , our assay has the advantage in short response time (within three minutes), high sensitivity (10 −12 g mL −1 for HBsAg) and stability.
研究领域:
生物化学
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医学检测
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
SENSORS
·
2018
·Vol. 18
·期 8
·2679
·
IF 2.475
Zheng-Jun Xie,Xian-Yu Bao,Chi-Fang Peng
A new colorimetric detection of methylmercury (CH3Hg+) was developed, which was based on the surface deposition of Hg enhancing the catalytic activity of gold nanoparticles (AuNPs). The AuNPs were functionalized with a specific DNA strand (HT7) recognizing CH3Hg+, which was used to capture and separate CH3Hg+by centrifugation. It was found that the CH3Hg+reduction resulted in the deposition of Hg onto the surface of AuNPs. As a result, the catalytic activity of the AuNPs toward the chromogenic reaction of 3,3,5,5-tetramethylbenzidine (TMB)-H2O2was remarkably enhanced. Under optimal conditions, a limit of detection of 5.0 nM was obtained for CH3Hg+with a linear range of 10–200 nM. We demonstrated that the colorimetric method was fairly simple with a low cost and can be conveniently applied to CH3Hg+detection in environmental samples.Keywords:methylmercury;gold nanoparticles;enzyme mimic;chromogenic reaction
研究领域:
环境科学
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纳米生物学
·
分析化学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2020
·
IF 0.936
Jia-Hui ZHAO,Guo-Yong LIU,Shuang WANG,Sha-Sha LU,Jian SUN,Xiu-Rong YANG
Dopamine is a crucial monoamine neurotransmitter involved in multiple cognitive functions and pathological processes. Herein, we described a rapid chromogenic and fluorogenic reaction between dopamine and naphthoresorcinol under ambient conditions, and found that the product possessed intense visible absorbance and high fluorescence quantum yield (78%) in aqueous solution. Through electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) analysis, the plausible reaction mechanism between dopamine and naphthoresorcinol was deduced, and the structure of the reaction product was confirmed. Inspired by the explicit mechanism and accessible reaction conditions, a straightforward and selective assay for dopamine concentration was developed with colorimetric and fluorescent dual-modal signals based on the in situ reaction.
研究领域:
生物化学
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分析化学
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神经科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Dan Huo,Xipeng Zhang,Jiaxin Wei,Jinhua Wang,Qiang Zhang,Qiulin Yang,Hongxiang Zhu,Fengshan Zhang,Guigan Fang,Ting Wu
The toxic volatile organic compounds (VOCs), especially formaldehyde (FA), released from decoration materials pose a great threat to human health. In this study, formaldehyde adsorption performance of the specially formulated nanocellulose/chitosan aerogel (CNFCA) was investigated in simulated atmosphere. The physicochemical property of the composite aerogel was characterized, which had a large specific surface area (153.67 m 2 /g), a rough surface and an ultra-thin and porous structure. The composite aerogel showed excellent adsorption capacity for the formaldehyde, its theoretical maximum adsorption capacity was as high as 83.89 mg/g, and the adsorption process was more in accordance with the pseudo-second-order kinetics. The chromogenic reaction between the 4-amino-3-benzo-5-mercapto-1,2,4-triazolium (AHMT) and CNFCA was found that the color of the composite aerogel was depended on the free formaldehyde concentration. Based on this phenomenon, a colorimetric card was proposed and built to detection the formaldehyde in the atmosphere. Moreover, the adsorption mechanism research was found that the CNFCA with a multilayer structure belonged to physicochemical complex adsorption.
研究领域:
材料科学
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环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Xue Wang,Bing Zhang,Jing Li,Honghong Chang,Wenlong Wei
Colorimetric assay is a powerful tool for detection of tumor markers with the outstanding advantages of visualization and simple analytical instruments. Herein, a simple and fast chromogenic reaction was developed based on Ag 3 PO 4 /Ag nanocomposite synthesized by one-step chemical bath method. And the Ag 3 PO 4 /Ag nanocomposite owned the ability of oxidizing of TMB under the acidic condition due to the hydrolytic action. The nanocomposite was characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), X-ray powder diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). Then, the colorimetric immunoassay was constructed by utilizing Ag 3 PO 4 /Ag nanocomposite as molecular labels for carcino embryonie antigen (CEA, UniProt accession number: P06731) detection. The blue color with gradient change was observed with the increasing of CEA concentrations. Under optimal conditions, the variation between absorbance values and CEA levels was linear in the range of 0.2 ng mL −1 ~ 2.5 ng mL −1 with the detection limit of 0.03 ng mL −1 . More importantly, the developed colorimetric immunoassay exhibited good selectivity , repeatability, stability and potential application in the real serum sample detection.
研究领域:
分析化学
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生物医学工程
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Miao Ma,Qing Wang,Qianqian Su,Weizhe Liu,Hui Xia,Caixia Zhou,Mengtian Li,Fang Yang,Fujian Xu
Blood uric acid (UA) levels are closely associated with various diseases. This study presents a novel UA photothermal detection system. UA reacts with uricase to generate hydrogen peroxide, which then oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) into oxidized TMB (oxTMB) in the presence of copper ions as the catalyst. Crucially, instead of expensive laser, Light Emitting Diode (LED) was applied to offer the irradiation on oxTMB, inducing a temperature increase. The increment of temperature measured by a handheld household thermometer exhibits a linear correlation with the concentration of UA in the samples. This method demonstrates enhanced detection sensitivity and excellent anti-interference capabilities. It has been successfully applied to precisely determine UA levels in volunteer blood samples. The sensing platform achieves portable UA detection in human blood within 3 min, featuring a linear response range of 50–400 μM, a detection limit (LOD) of 14.4 μM. This work establishes a novel miniaturized LED-based photothermal (LED-PT) uric acid detection device, holding significant potential for rapid, portable UA monitoring in healthcare and clinical diagnostics.
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产品关联
DOI
高影响因子
TALANTA
·
2013
·
IF 3.498
Rui Zhu,Yan Zhou,Xi-Liang Wang,Li-Ping Liang,Yi-Juan Long,Qin-Long Wang,Hai-Jie Zhang,Xiao-Xiao Huang,Hu-Zhi Zheng
It was found that Hg 2+ can inhibit the peroxidase mimetic activity of bovine serum albumin (BSA) protected Au clusters (BSA-Au) due to the specific interaction between Hg 2+ and Au + existed onto the surface of BSA-Au clusters. By coupling with 3, 3′, 5, 5′-tetramethylbenzidine (TMB)–H 2 O 2 chromogenic reaction, a novel method for Hg 2+ detection was developed based on the inhibiting effect of Hg 2+ on BSA-Au clusters peroxidase-like activity. This method exhibited high selectivity and sensitivity. As low as 3 nM (0.6 ppb, 3 σ ) Hg 2+ could be detected with a linear range from 10 nM (2 ppb) to 10 µM (2 ppm) and this method was successfully applied for the determination of total mercury content in skin lightening products.
研究领域:
分析化学
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生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 9
·1-16
·
IF 5.3
Meng Jingjing,Han Lu,Li Lu,Yin Xue-bo,Zheng Jing
Manganese dioxide (MnO 2 )-based heterogeneous catalysts have gained significant attention in nanozyme research due to their exceptional oxidase-mimicking capabilities. This work reports the rational design of MnO 2 functionalized magnetic carbon nanotubes (Fe 3 O 4 @C@MnO 2 ) through a multistep synthesis strategy. The magnetic N-doped hollow carbon substrate (Fe 3 O 4 @C) was first synthesized via controlled pyrolysis of FeOOH-coated polydopamine precursors. Subsequent interfacial redox reaction with KMnO 4 enabled the in situ deposition of ultrathin MnO 2 nanosheets on the nanotube substrate, forming a triple-component coaxial architecture. This hollow structure not only facilitates dense MnO 2 deposition but also maintains robust magnetic responsiveness. The obtained Fe 3 O 4 @C@MnO 2 exhibits remarkable enzyme-mimetic capability, catalyzing TMB chromogenic conversion with 0.92-fold enhanced catalytic performance relative to pristine MnO 2 . Systematic mechanistic studies reveal that tannic acid (TA) competitively inhibits ·OH radical generation via MnO 2 -TA complexation, while the carbon interlayer enhances electron transfer efficiency. Owing to the superior enzyme-mimetic performance of Fe 3 O 4 @C@MnO 2 and their inhibition effect by TA, a sensitive colorimetric biosensing assay was fabricated, achieving a TA detection limit of 0.029 μM with a 0.096–4.82 μM linear concentration range. This work exemplifies the synergy of structural engineering and component optimization in designing multifunctional nanozymes, showing potential applications in food quality monitoring, environmental analysis, and point-of-care diagnostics. Graphical
研究领域:
纳米酶技术
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纳米材料合成
·
生物传感器
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环境分析
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食品安全监测
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产品关联
DOI
PubMed
高影响因子
Information Processing in Agriculture
·
2022
·
IF 0
Xianbao Xu,Zhuangzhuang Bai,Tan Wang
Portable measurement of ammonia nitrogen in water is of great significance for water quality monitoring. It’s beneficial to reduce biological diseases and promote aquatic product safety. Traditional methods such as Nessler’s reagent method suffer from complex operation, time delays and toxic residues. To realize simple and pollution-free detection, this paper develops a low-cost portable device for ammonia nitrogen detection. A test paper was proposed to cooperate the device and offer a chromogenic reaction. The portable device reduces the impact of any ambient light, simplifies the operation, and provides human–computer interaction. The result obtained for the detection range of 0.4–10 mg/L (R 2 are 0.990 2 and 0.989 3 for the rang of 0.4–4.5 and 4.5–10 mg/L, respectively) with the detection limit of 0.36 mg/L, and the average recovery of aquaculture water is 100.98–137.75%. The results show that the portable device can provide a great potential for on-site detection ammonia nitrogen concentration.
研究领域:
环境科学
·
水质分析
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分析化学
·
水产养殖
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DOI
ACS Sensors
·
2022
·
IF 9.618
Kexin Fan,Jiayang Zeng,Chenyu Yang,Gonglei Wang,Kai Lian,Xiuhong Zhou,Yaping Deng,Guozhen Liu
Uric acid (UA) is an important biomarker for many diseases. A sensitive point-of-care (POC) testing platform is designed for the digital quantification of salivary UA based on a colorimetric reaction on an easy-to-build smartphone-assisted microfluidic paper-based analytical device (SμPAD). UA levels are quantified according to the color intensity of Prussian blue on the SμPAD with the aid of a MATLAB code or a smartphone APP. A color correction method is specifically applied to exclude the light effect. Together with the engineering design of SμPADs, the background calibration function with the APP increases the UA sensitivity by 100-fold to reach 0.1 ppm with a linear range of 0.1–200 ppm. The assay time is less than 10 min. SμPADs demonstrate a correlation of 0.97 with a commercial UA kit for the detection of salivary UA in clinical samples. SμPADs provide a sensitive, fast, affordable, and reliable tool for the noninvasive POC quantification of salivary UA for early diagnosis of abnormal UA level-associated health conditions.
研究领域:
医学诊断
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即时检测
·
生物化学
·
微流控技术
·
智能手机应用
·
显色分析
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 15
·3010-3015
·
IF 5.344
Yanbin Sun,Xianwen Wang,Linxin Fan,Xianli Xie,Zhaohua Miao,Yan Ma,Tao He,Zhengbao Zha
The famous chromogenic reaction between starch and iodine has been widely used for chemical analysis since its first discovery in the year of 1814 while it is seldom utilized in biomedical applications. Inspired by their high iodine content and strong optical absorbance in the near infrared (NIR) region, monodisperse amylose–iodine nanoparticles (AM–I NPs), synthesized by simple mixing of amylose NPs and KI–I2 solutions, were explored as a new class of high-performance antibacterial agent. Benefiting from the broad-spectrum antibacterial property of iodine and photothermal effect of the amylose–iodine complex, the obtained AM–I NPs exhibited an excellent photothermal-enhanced sterilization effect for both Gram-negative Escherichia coli (E. coli) and Gram-positive methicillin-resistant Staphylococcus aureus (MRSA). For instance, upon incubation with AM–I NP suspensions (30 μg mL−1) plus NIR laser irradiation (808 nm, 1.33 W cm−2, 5 min), the relative survival rates of E. coli and MRSA were only 1.2% and 1.7%, respectively. In addition, the AM–I NPs depicted better biocompatibility in vitro than that of KI–I2 solution, indicating their safety for potential biomedical applications in vivo. This proof-of-concept study revealed the antibacterial applications of a traditional starch-iodine complex and is expected to provide insights into the design and development of efficient broad-spectrum antibacterial agents.
研究领域:
生物医学工程
·
材料科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2020
·
IF 4.258
Yiming Zhang,Jinsheng Yu,Shuyu Lai,Jian Song,Xiaomei Wu,Dingnan Wang,Linjiang Pang,Tingting Chai
To construct a rapid read-out analytical platform for detecting histamine in foods , a self-visualization nanomaterial was prepared by stable doping of ninhydrin into TiO 2 nanoparticles ( [email protected] 2 ) using a sol-gel strategy, and it was printed onto the target zone for histamine on a high-performance thin layer chromatography (HPTLC) strip based on the Rf value. Under the optimum TLC conditions, histamine in the sample migrates to the target zone, and the colorimetric reaction occurs in situ . The resultant color spots can be either identified visually or semi-quantitatively by image analysis. Owing to the stable visualization performance, the constructed strip demonstrated good contrast, with a low background and easily recognizable colored spots. The linear range was 15.0–320.0 mg kg −1 , and the limit of detection for histamine in fish was 5.0 mg kg −1 . Other biogenic amines such as putrescine and cadaverine did not interfere with the analysis. Furthermore, analytical results for fish samples were verified by HPLC tests, demonstrating the robustness and reliability of the developed method. Therefore, the incorporation of a self-visualization nanomaterial in the HPTLC platform is a promising strategy for the rapid analysis of food contaminants in the future.
研究领域:
食品科学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）