Analytical Methods
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2026
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IF 2.6
lidi Gao,Jiawen Yu,Dongsheng Zhao,Xinyu Liang,Jun Wang,Fuquan Zhao,Ming Zhao,Hongtao Chu,Shili Qin
In this work, a novel Ti-based MOF (NH2-MIL-125) bonded open-tubular (OT) column was firstly prepared via one-step in-situ growth method for capillary electrochromatography (CEC). The stationary phase was characterized by Fourier transform-infrared spectroscopy, scanning electron microscopy, X-ray diffraction spectroscopy, and nitrogen adsorption-desorption measurements. The results showed that the stationary phase exhibited a large specific surface area (1247.57 m²/g) with microporous and mesoporous structure and no obvious changes in the morphology/size inside and outside the column. Baseline separation of three β-adrenergic receptor blockers (Prop, Sot, and Lab), three β-adrenergic receptor agonists, and four sulfonamide antibiotics were obtained under the optimized CEC conditions with the shortest analysis time of 2.52 min and a maximum resolution of 11.52. The separation mechanisms were mainly attributed to polarity and electrophoretic mobility of the analytes, as well as π-π interactions and hydrogen bonding interaction between the stationary phase and the analytes. A quantitative detection method of three β-adrenergic receptor blockers in pork samples was established using the NH2-MIL-125 bonded OT column. Good linearity (R² > 0.999) was obtained over the concentration range of 0.01-1.00 mg/mL with limits of detection of 0.0044-0.0084 mg/mL and recoveries of 90.27-106.74%. Thus, the developed method was simple, rapid and high efficiency, and could be applied for the simultaneous separation and detection of the three β-adrenergic receptor blockers in real samples.
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JOURNAL OF ETHNOPHARMACOLOGY
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2026
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IF 6.8
Li-Hua Zuo,Sheng-Jie Yang,Zhi-Bo Zhang,Chen-Han He,Yu-Jie Wei,Ya-Ran An,Song-Feng Zhao,Zhi Sun,Li-Wei Liu
Ethnopharmacological relevance Gualou Xiebai Banxia decoction (GXB) is a classic formula originated from “Synopsis of the Golden Chamber”, with efficacy in activating cardiac “yang”, resolving phlegm, and promoting blood circulation. Nearly 2000 years of clinical practice have demonstrated the efficacy of GXB in managing myocardial ischemia (MI). Nevertheless, its regulatory effects on metabolic disturbances associated with MI and the underlying mechanisms remain obscure. Purpose This study aims to evaluate the therapeutic effects of GXB against MI, characterize the metabolomic and lipidomic alterations related to GXB treatment, and investigate the potential mechanisms of action. Methods UHPLC-Q-Orbitrap HRMS combined with network pharmacology were implemented to explore the active ingredients and potential targets of GXB in the treatment of MI. Subsequently, an isoprenaline (ISO)-induced rat model of MI was established to assess the cardioprotective effects of GXB using echocardiography, biochemical indicators, and histopathological analysis. Comprehensive metabolomics and lipidomics were conducted to characterize endogenous metabolic alterations following GXB intervention. A components-targets-differential metabolites network was constructed through integrated analysis. Further molecular biology experiment verification was performed in ISO-induced rat model and H9c2 cell injury model. Notably, inhibitors were used in H9c2 cells to validate the role of PLA2 and PKC in GXB's protective effects. The levels of key proteins, oxidative stress markers and inflammatory cytokines were detected. Results A total of 123 constituents were identified in GXB, with 18 detected in plasma. The “constituents-targets-disease” interaction network was constructed based on absorbed components. GXB improved cardiac function, alleviated cardiac injury, reduced oxidative stress and inflammation in MI rats. Metabolomic and lipidomic analyses revealed 19 differential metabolites and 122 lipids associated with GXB treatment, primarily involved in lipid metabolism, amino acid metabolism, and energy metabolism. Conjoint analysis identified four key targets, including PLA2G2A, PLA2G4A, PRKCA, and PRKCE, which were mainly linked to lipid metabolism. GXB treatment decreased the expressions of these four proteins, reduced oxidative stress and inflammation. In vitro functional experiments confirmed that inhibition of PLA2 and PKC proteins mimicked the protective effects of GXB. Conclusion This study demonstrates that GXB protects against MI by regulating lipid metabolism and attenuating inflammation via inhibiting the PLA2/PKC-NOX signaling axis, providing evidence for its clinical application.
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Journal of Environmental Chemical Engineering
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2022
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IF 7.968
Fan-xin Kong,Lian You,Jia-mei Cao,Qian Liu,Ping Xia,Jin-fu Chen
Holey graphene oxide (HGO) and reduced HGO membranes (rHGO) can overcome high tortuosity and poor stability of GO-based membranes, respectively. In this study, HGO and rHGO membranes were fabricated to try to quantitatively elucidate the etching and reduction time on structure, stability and rejection performance for slat and pharmaceuticals. Water flux of HGO membrane linearly increased ( J v =2.07 t + 1.02, R 2 =0.9931) and Na 2 SO 4 rejection linearly decreased ( R Na2SO4 =−4.37 t + 88.26, R 2 =0.9511), while the membranes stability substantially increased with etching time from 1 to 5 h (water flux variation from 22% to 5% and rejection ratio variation from 17% to 7%) under long-term vibration . Subsequently, water flux of rHGO membranes exponentially decreased ( J v = exp (2.89–0.12 t + 0.0039 t 2 ), R 2 = 0.9996) with reduction time, while slightly improved salt rejection ( R Na2SO4 = exp (2.89–0.12 t + 0.0039 t 2 ), R 2 = 0.9971) were observed. Effective length of actual pathways for GO, HGO 4 and rHGO 4–5 membranes calculated by the mass transfer-based model was 7.37 ± 0.47, 4.48 ± 0.68 and 7.23 ± 1.29 µm, respectively, which were much higher than that geometric thickness of the GO membranes. These results indicated that d -spacing, tortuosity and porosity should be comprehensively considered for membrane design and fabrication. With a series of saccharides as probes, average pore size slightly decreased for HGO 4 ( r p =0.36 nm, S p =0.34) and rHGO 4–5 ( r p =0.33 nm, S p =0.28) membrane compared with GO membranes ( r p =0.38 nm, S p =0.3), demonstrating that etching and following reduction marginally affected the pore size distribution. H 2 O 2 etching increased both water permeability ( A) and its selectivity ( A/B ) for three pharmaceuticals, while the thermal reduction substantially decreased the water flux. Moderate and low rejection ratios for the three pharmaceuticals also demonstrated the importance of trade-off between etching and reduction time and the urgent need for narrowing pore-size distribution for GO-based membranes for water purification .
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Journal of Materials Chemistry B
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2022
·Vol. 10
·期 35
·6742-6751
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IF 7.571
Long Jiang,Tao Jing,Xiantao Shen
The development of an elution-free solid-phase extraction (SPE) process is of special interest in sample pretreatment. Due to the phase-change merits at relatively low temperatures and easy dissolution in n-hexane, wax spheres show great potential in this field. However, the conventional wax spheres possess a low affinity towards the target analytes when they are used as SPE adsorbents. In this study, using octadecanoic acid as the functional monomer and wax as the matrix, molecularly imprinted wax (MIW) spheres were successfully prepared. The obtained MIW spheres displayed remarkable molecular recognition ability and high selectivity towards the template. Interestingly, the as-synthesized molecularly imprinted wax (MIW) could be dissolved in n-hexane or melted by heating for subsequent fluorescence and mass spectrum analysis without the target elution process. Moreover, the melted MIW exhibited high repeatability, sensitivity and specificity for solid-state fluorescence detection. We believe that the imprinting method presented in this study will open a new window in analytical chemistry.
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FOOD AND CHEMICAL TOXICOLOGY
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2023
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IF 4.3
Kaixuan Li,Guode Zhao,Lan Yang,Xingyu Gao,Yudi Jia,Yang Wang,Xiaohong Zhang,Weiwei Li,Ying Peng,Jiang Zheng
4-Methylquinoline (4-MQ) is a quinoline derivative widely present in groundwater and soil and has been reported to be genotoxic. The mechanisms of the toxic action remain unknown. This study aimed to elucidate the metabolic activation of 4-MQ and to determine the possible role of reactive metabolites in 4-MQ-induced liver injury in rats. In the present study, a hydroxylation metabolite (M1), a GSH conjugate (M2) and an NAC conjugate (M3) derived from 4-MQ were detected in vitro and in vivo . The structures of the two conjugates were verified by chemical synthesis, mass spectrometry, and nuclear magnetic resonance. CYP3A4 was found to dominate the hydroxylation of 4-MQ. Sulfotransferases also participated in the metabolic activation of 4-MQ. Pretreatment of primary hepatocytes with ketoconazole (KTC) or 2,6-dichloro-4-nitrophenol (DCNP) not only reduced the production of GSH conjugate M2 but also decreased the susceptibility of hepatocytes to the cytotoxicity of 4-MQ. Urinary NAC conjugate M3 was found in rats given 4-MQ, and M3 may be a potential biomarker for 4-MQ exposure.
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Pharmacogenomics & Personalized Medicine
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2021
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IF 3.912
Magesa Mafuru,Sanlan Wu,Henry Mayala,Zaituni Msengwa,Amani Phillip,Charles Mgone
Purpose To analyze the combined effect of CYP2C19 genetic polymorphism and PPIs coadministration on voriconazole trough concentration (VCZ-Ctrough) in Chinese patients with hematological disorders.Patients and Methods A prospective observational study involved 250 plasma samples from 114 adult patients receiving voriconazole with or without PPIs were analyzed. Demographics and clinical characteristics were obtained from patient’s records. A validated LC-MS/MS was used to quantify the plasma VCZ-Ctrough. Genotyping for CYP2C19*2 and CYP2C19*3 variant alleles was performed by PCR-RFLP followed by DNA sequencing. The combined total score (from 2 to 5) was calculated for each patient. The higher the score, the lesser the metabolism of the patient.Findings Fifty percent of patients administered with voriconazole were coadministered with PPIs, predominantly omeprazole or esomeprazole. Patients exhibiting CYP2C19 poor metabolizer phenotype showed a significantly higher median VCZ-Ctrough, (4.31µg/mL [IQR, 1.64µg/mL–7.36µg/mL]) than patients with normal metabolizer (1.38µg/mL, [IQR, 0.79µg/mL–2.14µg/mL], p < 0.0001). Similarly, patients co-administration with PPIs had higher median VCZ-Ctrough (2.86µg/mL [IQR 1.33µg/mL–4.66µg/mL]), than PPIs non-users (1.71µg/mL, [IQR, 0.86µg/mL–3.48µg/mL], p = 0.001). However, we noted that the median VCZ-Ctrough for each factor was ranging within the normal recommended therapeutic range in the Chinese population (0.5µg/mL–5µg/mL). But when the two factors were combined, the median VCZ-Ctrough was steadily increasing as the metabolic capacity (reflected by combined total score) was increasing. Importantly, the median VCZ-Ctrough in PM/PPIs user (total score 5) was significantly elevated to supra-therapeutic levels compared to NM/PPI non-user group (total score 2) (5.83µg/mL [IQR, 2.19µg/mL–9.51µg/mL] versus 1.13µg/mL [IQR, 0.67µg/mL–1.82µg/mL]), respectively, P < 0.0001. Furthermore, we observed that the elevation of median VCZ-Ctrough to supra-therapeutic levels was largely contributed by omeprazole or esomeprazole compared to lansoprazole or pantoprazole.Conclusion Coadministration with PPIs significantly increased voriconazole trough concentrations and there was an additive effect in CYP2C19 PMs, who were most likely to have supra-therapeutic levels.
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JOURNAL OF DRUG TARGETING
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2026
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IF 4.7
Weishuo Ju,Ran Zhang,Jingxuan Guo,Shuo Li,Huimin Zhang,Huijia Song,Yanping Sun,Zibin Gao
Accurately and efficiently assessing drug permeability has emerged as a critical challenge in early drug discovery and development, prompting the development of various models. In this study, a polymer planar biomimetic membrane permeation assay (PPBMPA) model was established using a Transwell chamber, and its permeability assessment capability was evaluated using model drugs with high, medium, and low permeability. The underlying permeation mechanism was also investigated. The results demonstrated that the biomimetic membrane spontaneously differentiated into an intestinal-like structure comprising a hydrated layer, a polymersome layer, and a basal layer, thereby mimicking the sequential process of drug permeation across the mucus layer, intestinal epithelium, interstitial space, and into the bloodstream. This highly biomimetic permeation pattern enabled effective differentiation between high and low absorption drugs, exhibiting a strong correlation with Caco-2 permeability (R2 = 0.85). Collectively, these findings suggest that the novel PPBMPA model holds promise as a reliable tool for rapid and reproducible drug permeability prediction.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2023
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IF 4.4
Xuanping Tan,Yuhui Zhang,Huaping Mao,Jidong Yang
In this work, specific aptamers with affinity for S-propranolol were screened by SELEX technology based on the graphene oxide (GO) adsorption platform, and a GO-FAM labeled aptamer-propranolol fluorescent optical switch system was constructed for the recognition of chiral propranolol. It was found that the fluorescence quenching of FAM labeled aptamer could be caused by the adsorption of GO. However, when S-propranolol was introduced, S-propranolol could pull out the aptamer adsorbed by GO, and the fluorescence of the system could be restored. But, R-propranolol could not be realized. Therefore, a simple and sensitive fluorescent optical switch system was established to identify chiral propranolol and perform highly sensitive detection of S-propranolol.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2021
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IF 7.312
Xin Liu,Peng Xu,Qi Fu,Runhan Li,Chenxi He,Wenxuan Yao,Lei Wang,Shiqi Xie,Zhiyi Xie,Jingwei Ma,Qiulai He,John C. Crittenden
Zero-valent copper activated peroxymonosulfate (ZVC/PMS) system was widely used to degrade refractory contaminants due to being highly effective. However, the low reactivity and easy dismutation of cuprous species (Cu(I)) in acid condition limited its efficiency. In our study, ZVC coupled with ferrous ion (Fe(II)) promoted PMS to degrade orange II. Multiple oxidants including Cu(III), OH and SO 4 − made main contribution on orange II degradation in the Fe(II)/ZVC/PMS system. In the Fe(II)/ZVC/PMS system, ZVC generated Cu(I) by H + corrosion and PMS oxidation, and Cu(I) reacted with oxygen producing hydrogen peroxide. Then, Fe(II) and Cu(I) catalyzed hydrogen peroxide and PMS to generate Cu(III), OH and SO 4 − , and thereinto Fe(III) was reduced to Fe(II) by ZVC and Cu(I). In the Fe(II)/ZVC/PMS system, water matrix including humic acid, sulfate and nitrate had no significant impact on orange II degradation, while chloride restrained the degradation of orange II. The Fe(II)/ZVC/PMS system exhibited great degradation for orange II in tap water, lake water and well water. Additionally, the Fe(II)/ZVC/PMS system degraded more than 90% of methyl orange, rhodamine B, acetaminophen, ibuprofen, propranolol and diclofenac. Through adjusting the final reaction solution to pH 8.5 and subsiding, the discharge of total copper met the II-level environmental quality standards for surface water in China (1 mg/L). Hence, the study provided a promising method to induce PMS for enhanced degradation of contaminants.
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Environmental Science-Nano
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2021
·Vol. 8
·期 7
·1978-1991
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IF 8.131
Ming Yan,Yilin Wu,Rongxin Lin,Faguang Ma,Zhongyi Jiang
Although many researchers have done several studies on improving the selective separation performance of membrane materials, the conceptions and applications of membrane-based molecular imprinting separation and recognition with both high permselectivity and rebinding capacity have still faced challenges. Herein, for the first time, the bioinspired polydopamine (PDA)-modified porous regenerated cellulose membrane (pRCMs) with Au-based imprinting structure was constructed for the synthesis of molecularly imprinted nanocomposite membranes (MINMs); propranolol was used as template molecule. Importantly, based on the as-constructed PDA-modified layers, abundant Au-modified particles have been uniformly distributed onto the surfaces of membranes, and Au/PDA-based structures can be used as the imprinted-initiated factors for the preparation of sandwich-like-imprinted sites by developing a two-step temperature propranolol-imprinting method. Moreover, superior rebinding capacity (76.87 mg g−1) and selectivity (4.27–4.56) of MINMs has been successfully achieved; the as-prepared MINMs also showed excellent regeneration and permselectivity of propranolol (16.01–18.34) by constructing large amounts of propranolol-imprinted sites on the Au/PDA-based surface. Therefore, under optimized conditions, MINMs would finally obtain reproducible, accessible, and stable membrane-based separation system of the propranolol molecule.
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FOOD CHEMISTRY
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2021
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IF 7.514
Lanteng Wang,Jin Wang,Ang Zhang,Xin-an Huang,Hongtao Lei
A growing number of β -agonists are illegally using for reducing animal fat deposition in animals, but the development of analytical methods always lags behind the emergence of new illegal compounds. Therefore, class specificity antibody-based immunoassays that can detect a great many β -agonists are important for timely supervision. In this study, a competitive inhibition enzyme-linked immunosorbent assay (ciELISA) based on a clenbuterol monoclonal antibody was developed to recognize 23 β -agonists and analogues. Holographic and three-dimensional quantitative structure–activity relationship (HQSAR and 3D QSAR) revealed that there are two critical binding epitopes on β -agonist hapten affecting antibody specificity, and these epitopes have been further validated using a ractopamine antibody with narrow specificity. Tert -butyl at C-2′ epitope is needed to generate class specific antibodies, and different characteristics of substituents at benzene ring epitope would adjust antibody specificity. This investigation could provide reference for future design of β -agonist haptens.
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Analytical Methods
·
2019
·Vol. 12
·期 2
·141-148
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IF 2.378
Xue Ren,Man He,Beibei Chen,Bin Hu
Herein, magnetic porous organic polymers (MOPs) prepared via an azo coupling reaction in water under mild conditions are evaluated for the extraction of seven cardiovascular drugs, including xylocaine, metoprolol, mexiletine, labetalol, propranolol, carvedilol and propafenone, from human urine samples for the first time. The extraction mechanisms are assumed to be electrostatic interaction and π–π stacking. The MOPs exhibited fast adsorption/desorption time (13 min) and high enrichment factors (315–365-fold) for target analytes. Based on this, a method coupling magnetic solid phase extraction with HPLC-UV detection was developed for the analysis of cardiovascular drugs. Under the optimized conditions, the LODs (S/N = 3) were in the range of 0.06–0.57 μg L−1 with an RSD (n = 5) of 4.3–8.6%, and the linear range was 0.2–200 μg L−1 for propranolol, 2–200 μg L−1 for metoprolol, and 0.5–200 μg L−1 for the other five analytes. The developed method was applied for the analysis of cardiovascular drugs in urine samples with recoveries of 89.0–112%. In order to further evaluate the application potential of this method, real-time monitoring of metoprolol in urine samples collected from volunteers treated with metoprolol tablets was performed. The proposed method is simple, fast, and sensitive and has good potential in the monitoring of cardiovascular drugs in competitive sports and clinical analysis.
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