INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2017
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IF 3.671
Ping Shao,Ben Niu,Hangjun Chen,Peilong Sun
Edible packaging films using polymer for food preservation have been developed for a long time. In this study, the effects of different concentrations (0.5%, 1%, 1.5%, w/v) of tea polyphenols incorporated into pullulan-Carboxymethylcellulose sodium (Pul-CMC) solutions on electrospun nanofiber films were evaluated. The fiber size distribution was characterized by scanning electron microscopy. The morphological features of nanofibers were modulated through adjusting process parameters (e.g. concentration of polymer solution, applied voltage and feeding rate). Increasing the applied voltage from 19 to 21 kV and the feed rate from 0.36 to 0.6 mL/h leads to a reduction in mean fiber diameter. Fruit packaging potential was evaluated using strawberry. The pullulan-CMC-TP nanofibers significantly decreased weight loss and maintained the firmness of the strawberries, and improved the quality of the fruit during storage. The findings demonstrate a facile packaging route to improve food sustainability and reduce waste.
研究领域:
食品科学
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材料科学
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高分子化学
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农产品贮藏与加工
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功能食品
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FOOD RESEARCH INTERNATIONAL
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2026
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IF 8.8
Wei Liu,Lin Wang,Kunyi Wang,Xinzhi Liang,Ting Liu,Jingxuan Tan,Xiaoyong Qiu,Jingcheng Hao
Double-layer fibers control gaseous microenvironment, suppressing respiration. • Double-layer fibers possesses high selectivity of CO 2 and O 2 . • The dual mechanism—gas regulation and bioactive—prolongs the quality of fruits. Respiration is the primary driver of postharvest deterioration in highly perishable fruits, as both plant tissues and associated microorganisms rapidly consume available nutrients and accelerate quality loss. Modulating the surrounding gaseous environment is therefore critical for mitigating respiration-the most important factor of driving spoilage. In this study, double-layer fiber films with selective gas permeability were developed to regulate O 2 and CO 2 levels within localized microenvironments. Their asymmetric structure established a mildly hypoxic and CO 2 -enriched atmosphere, achieving a gas selectivity of 11.39 ± 0.88 and effectively lowering respiratory intensity. The fibers also provided antibacterial and antioxidative functions, contributing to reduced microbial contamination and oxidative degradation. When applied to litchi, the films extended the effective commercial shelf life to approximately 9 days and significantly delayed overall spoilage throughout a 15-day evaluation period under controlled storage conditions. Beyond their demonstrated efficacy, these fibers show considerable potential for advancing food preservation strategies, offering meaningful opportunities for more sustainable packaging solutions. Their dual-action mechanism—integrating gas regulation with biological active—positions them as a versatile material platform for addressing spoilage challenges in highly perishable fruits. Download: Download high-res image (89KB) Download: Download full-size image
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Current Research in Food Science
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2026
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IF 7.7
Jianwei Dong,Jiayue Ma,Yuchen Li,Huan Wang,Jiaxing Huang,Hao He,Gege Zhang,Jingzhang Geng
The rational selection of stabilizers for selenium nanoparticles (SeNPs) is hindered by a lack of mechanistic understanding. Accordingly, this study investigated the deeper polysaccharide-based stabilization mechanism by comparing the effects of linear pullulan polysaccharide (PP), branched amylopectin starch (AS), and network-soluble β-cyclodextrin polymer (SP) on the growth of SeNPs. Growth kinetics showed that bare-SeNPs and SP-SeNPs followed a reaction-limited growth pathway, yielding larger particles due to weak diffusion restriction. In contrast, PP and AS induced a diffusion-limited growth regime, effectively inhibiting Se nucleus diffusion and collision, thus forming small, uniform SeNPs. FT-IR, XPS, TGA and ITC confirmed the interaction strength between the three polysaccharides and SeNPs was AS > PP > SP, correlating with their stabilization capability. AS-SeNPs displayed the smallest size and the highest stability, maintaining high stability even under low-pH and alcohol conditions, likely due to steric hindrance. It is predicted that the shelf life of selenium-enriched Huangjiu supplemented with AS-SeNPs could reach two years. In summary, polysaccharides govern SeNPs growth primarily by restricting nucleus diffusion, supported by interfacial interactions. Steric-hindrance-based stabilizers, such as AS, are particularly suitable for complex food systems, providing a theoretical basis for rational stabilizer selection in selenium-enriched foods.
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CARBOHYDRATE POLYMERS
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2026
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IF 13.2
Sandeep Karki,Sahil Malhotra,Muhammad Ijaz,Nicolas Rollet,Eoin D. O'Cearbhaill,Estela Bini,David J. Brayden
The buccal route has re-emerged as an alternative non-injectable route for peptide delivery. However, buccal peptide bioavailability is low, necessitating new formulation strategies. Pullulan, a water-soluble carbohydrate polymer, offers potential as a film-forming agent for buccal peptide delivery. Our aim was to co-administer a glucagon-like peptide-1 receptor agonist (GLP-1 RA) model analogue with an epithelial permeation enhancer, sodium glycodeoxycholate (GDC) in a bilayer film using a pullulan-based mucoadhesive layer. A pullulan–carboxymethyl cellulose mucoadhesive layer was selected, along with a backing layer made from an ammonio-methacrylate copolymer (Eudragit® RLPO). Physicochemical characterization confirmed the suitability of the pullulan-based film for buccal application. A Hilltop Chamber comprising an adhesive-backed cotton pad identified an optimal GLP-1 RA:GDC ratio for peptide permeation across ex vivo porcine buccal mucosae. 200 mM GDC (94.4 mg/mL) enabled ∼6% flux at 3 h using a donor concentration of 50 mg/mL GLP-1 RA (∼1:2). When these concentrations were incorporated into the prototype pullulan films, GLP-1 RA fluxes across mucosae reached ∼4.5% and ∼2% at 3 h for single mucoadhesive-layer and bilayer films, respectively, without overt tissue damage. Integrating GLP-1 RA and GDC into a pullulan–based bilayer film offers a promising carbohydrate polymer platform for enhancing buccal peptide delivery.
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ACS Applied Polymer Materials
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2026
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IF 5.2
Yaqi Zhang,Shiyao Liu,Hongman Xu,Jiahui Wu,Ruquan Zhang,Yongzhen Tao
Polyanionic polysaccharide-based hydrogels were developed as multistimuli-responsive drug carriers. Methacrylated pullulan (MP) acted as a thinner and rigidity regulator. Carboxymethylated-methacrylated xanthan gum (CMMXG) provided negative charges, ionic conductivity, and viscoelasticity. The cumulative ciprofloxacin hydrochloride (CPFXH) release mass dropped dramatically, whether the hydrogel was set at the anode or cathode with a potential of 3 V, due to irreversible electrochemical oxidation. Hydrogels with lower drug-loading amounts exhibited higher release efficiency when an electric stimulus was applied in the initial 5 h. A high CPFXH-loading amount led to rapid diffusion and quick electrochemical oxidation. For CMMXG3-MP7.5-CPFXH1680-LAP, the CPFXH release efficiency ratio of 3 to 0 V decreased from 1.33 ± 0.43 to 1.01 ± 0.06 over a duration of 0.5 ∼ 2 h, further indicating that the encouraged release and electrochemical degradation occurred simultaneously. The CPFXH release efficiency in phosphate buffer saline (pH = 7.4) was 1.5 times higher than that in simulated gastric fluid (pH = 1.2), and the CPFXH diffusion coefficient with loading weight was 6 times higher than that without loading, indicating that it was sensitive to pH and compressive force. The hydrogels exhibited electric, pH, and mechanical stimuli-responsiveness due to the presence of charged groups along the chains and viscoelasticity with robustness enough to respond and withstand compressive loading.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2026
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IF 6
Sahil Malhotra,Sandeep Karki,Holly Linford,Muhammad Ijaz,Jimmy Muldoon,Ehsan Rezvani,Antonio Benedetto,Arun HS Kumar,Eoin O’ Cearbhaill,David J. Brayden
The peptide, octreotide, is used to treat acromegaly and neuroendocrine tumours. It is administered by either a long-acting injection or by an oral capsule. Although the oral route is preferrable, food interference necessitates dosing octreotide on an empty stomach and bioavailability is only ∼0.7%. Buccal film administration avoids the fasting constraints of oral octreotide and offers the potential for improved systemic bioavailability. This study presents a Quality by Design (QbD)- informed strategy to create a mucoadhesive pullulan-based buccal film co-entrapping octreotide with a bile salt permeation enhancer, sodium glycodeoxycholate (GDC). Pullulan was selected from a panel of candidate polymers based on film-forming performance and compatibility with octreotide and GDC, enabling reproducible formulation at relatively high entrapment levels despite the tendency of them to associate. Atomic force microscopy of films revealed concentration-dependent nanoscale roughness and microdomain formation at high GDC and octreotide levels. However, these structural features did not compromise key critical quality attributes (CQAs) including mechanical robustness, rapid hydration/disintegration, and acceptable content uniformity. Critical performance attributes (CPAs) using isolated porcine buccal mucosae demonstrated that films containing 10% w/w GDC (relative to pullulan) enhanced tissue uptake and octreotide permeation and via reversible non-destructive epithelial modulation. Stability testing confirmed >90% chemical recovery and conformational integrity of octreotide in films. UHPLC–HR-MS/MS confirmed identical fragmentation patterns for film-released and native octreotide, confirming the peptide’s intact cyclic backbone and the absence of chemical modification after formulation and after >60 days storage at ambient environment. This is the first report to integrate nanoscale structural analysis with systematic QbD- informed evaluation of formulation quality and performance, establishing pullulan–GDC buccal films as a robust adaptable platform for future multilayer oromucosal peptide film development.
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BIOCHEMICAL ENGINEERING JOURNAL
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2026
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IF 3.6
Wenyong Wang,Juanjuan Liu,Shiwen Xu,Chao Yuan,Dongdong Meng
The debranching of α-1,6-glucose branch-points in starch by isoamylase is essential for in vitro enzymatic cascades containing α-glucan phosphorylase (αGP) that convert starch into valuable compounds. Herein, a highly active isoamylase, GK2827, from thermophilic Geobacillus kaustophilus was enzymatically characterized with a half-life of 46.6 h at 65°C. GK2827 exhibits a specific activity of 848 U/mg toward maltodextrin (dextrose equivalent 8.6), which is 31.8-fold higher than that toward pullulan. Molecular dynamics simulations revealed that a combination of favorable electrostatic interactions, stronger covalent binding, and stable van der Waals interactions enhance the binding affinity of GK2827 for maltodextrin compared to its affinity for pullulan. In an enzymatic cascade comprising αGP, α-glucosidase and cellobiose phosphorylase for cellobiose synthesis in one-pot, the inclusion of GK2827 increased the maltodextrin utilization rate from 52.6% to 80.9%. This excellent heterologously expressed isoamylase facilitates αGP-based starch phosphorylation in biomanufacturing various functional saccharides via in vitro enzymatic cascade.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Lin Wang,Wei Liu,Xianrui Chen,Zhenzhen Wu,Nitong Bu,Huimin Xiao,Chen Ma,Jingcheng Hao
Food spoilage and waste present significant global challenges, highlighting the need for innovative and sustainable packaging solutions. This study introduces a biodegradable Janus nanofiber, designed with dual-sided water impermeability, carefully engineered wettability gradients, and multiscale structural features. The design integrates moisture management with microbial control, creating an optimal microenvironment for the preservation of tropical fruits. The nanofiber retains moisture on its hydrophilic side while preventing external moisture ingress through the hydrophobic side, effectively reducing weight loss and extending shelf life. In 15-day shelf-life tests at room temperature and 4 °C, the nanofiber outperformed commercial packaging options, reducing fruit weight loss by >20%, while maintaining texture, firmness, and vitamin C content, and significantly delaying microbial spoilage. These improved results are supported by multiscale dissipative particle dynamics simulations, which demonstrate the material's ability to control moisture transport and suppress microbial growth. This work offers a scalable and high-performance solution for biodegradable antimicrobial packaging, enhancing sustainable food preservation practices. The novel 3D PHB-AT NPs show strong potential for tropical fruit preservation and can be extended to other sustainable food storage applications, addressing major challenges in the global food supply chain.
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FOOD CONTROL
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2026
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IF 7
Lin Wang,Wei Liu,Xianrui Chen,Zhenzhen Wu,Zeyi Wang,Xiuqing Guan,Huaiwen Zhang,Jingcheng Hao
Postharvest quality retention relies on packaging materials capable of concurrently regulating microbial and moisture microenvironments. In this study, an asymmetric bilayer nanofiber membrane was engineered to integrate natural deep eutectic solvents (NADESs) and polyphenol-functionalized silver nanoparticles (PF-Ag) for coordinated regulation of these preservation pathways. NMR confirmed the hydrogen-bonded structure of the NADESs, while SEM, TEM, and FTIR verified the morphology and surface chemistry of PF-Ag. These active units were embedded within hydrophilic and hydrophobic fibers and assembled into a wettability-asymmetric bilayer via electrospinning. The NADESs improved interfacial compatibility and fiber uniformity, whereas PF-Ag imparted effective microbial suppression and radical-quenching capacity. The bilayer architecture also enabled directional moisture transport, thereby establishing a tightly regulated microbial and moisture microenvironment. Compared with single-layer or additive-free controls, the membrane exhibited markedly improved mechanical stability, gas barrier performance, and moisture management. Applied to fresh prunes, the membrane effectively slowed microbial spoilage, mitigated surface moisture accumulation, stabilized internal gas composition, and extended shelf-life to 15 days at ambient temperature. This work elucidates a synergistic NADES–PF-Ag strategy for microenvironment-responsive bilayer nanofibers and provides a promising platform for sustainable postharvest packaging.
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JOURNAL OF FOOD PROCESS ENGINEERING
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2026
·Vol. 49
·期 1
·e70336
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IF 2.9
Jing Li,Kang Yang,Xiaoqin Shi,Tao Li,Lang Guo,Yong Guo,Xinhui Wang
In this study, carvacrol (CA)/thymol (TH) nanoemulsions (CT NEs) were first prepared by the ultrasonic emulsification method, and then the effects of various nanoemulsion concentrations on the physicochemical properties and biological activities of chitosan/pullulan (CS/PU) nanocomposite films were systematically evaluated. The CT NEs exhibited good stability with a high encapsulation efficiency of 90.29% ± 0.95%, and they were well integrated in the CS/PU matrix as confirmed by the microstructure characterization. The performances of the nanocomposite films were significantly enhanced by CT NEs, including the improvements of mechanical properties and barrier properties. The CS/PU-CT films exerted notable antioxidant and antibacterial activities. In the end, the CS/PU-3CT film (with 3.0% w/v CA and TH), with the best antioxidant and antibacterial performance, was applied to the preservation of pork, and its shelf life was extended for 10 days compared to the CK group. The film loaded with 3CT NEs exhibits outstanding performance and offers significant prospects for use in food packaging.
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Sustainable Materials and Technologies
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2026
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IF 9.2
Peiyuan Li,Wenlin Zhou,Qing Guo,Siyan Liang,Chumei Liang,Wei Su
The development of multifunctional wound dressings from sustainable resources is critical to advancing green biomedical materials for effective wound repair. In this study, we present an eco-friendly microneedle delivery platform (LutCe-QCS-GP MN) with integrated reactive oxygen scavenging and antibacterial functions. The system features a sustainable nanozyme (LutCe), formed by self-assembly of the plant-derived flavonoid luteolin and cerium ions, which exhibits robust superoxide dismutase-like activity. These LutCe nanozymes organic-inorganic hybrids were synthesized through a green assembly process and demonstrate potent scavenging capability against superoxide anions, hydroxyl radicals, and reactive nitrogen species. Incorporated into the tips of the microneedles, they directly alleviate oxidative stress at the wound site. Simultaneously, an antimicrobial polysaccharide-based matrix provides effective defense against wound pathogens, preventing infection and secondary tissue damage. By synergistically combining sustainable antioxidant nanozymes with natural antibacterial components, the LutCe-QCS-GP MN system significantly reduced oxidative stress and bacterial load in wounds, markedly accelerating the healing process. Comprehensive in vitro and in vivo safety evaluations support the biosafety of the platform, underscoring its potential for clinical translation. This work not only introduces a promising therapeutic strategy for wound management but also highlights the valorization of natural, sustainable resources in functional material design, offering a meaningful reference for green technology applications in biomedicine.
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Frontiers in Pharmacology
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2026
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IF 4.8
Huabing Yuan,Lu Han,Hao Deng,Yue Tan,Yi Yang,Yi Liu,Jiaxin Ying,Mengqi Fang,Hui Wei,Zhihe Tao
Brain-targeted drug delivery remains a major challenge in pharmaceutical research. In this study, four types of polysorbate 80 and cholesterol-modified pullulan polymers (CHPP) were synthesized and self-assembled into nanoparticles (CHPP NPs) for brain drug delivery. The properties of these NPs, including brain targeting efficiency, were systematically evaluated to investigate the influence of polymer modification on their performance. The hydrophobicity of the polymers increased with the degree of cholesterol substitution, which in turn led to a reduction in nanoparticle size. Furthermore, higher hydrophobicity contributed to an increased drug loading capacity for paclitaxel (PTX) and a more sustained drug release profile. In vivo fluorescence imaging revealed that formulations with higher levels of polysorbate 80 and cholesterol modification exhibited significantly enhanced brain targeting efficiency compared to other variants. The PTX-loaded CHPP NPs (PTX-CHPP NPs) demonstrated potent cytotoxicity and inhibitory effects against SJ-GBM2 glioma cells in vitro, underscoring their potential as a promising platform for brain-targeted drug delivery. These findings provide valuable insights for the rational design of brain-targeting nanocarriers.
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