BIORESOURCE TECHNOLOGY
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2026
·
IF 8.2
Weilong Peng,Lijun Wang,Lichao Song,Yan Liu,Yu Ren,Yaya Wang,Weimei Wang,Jun Chen,Ruonan Bo,Mingjiang Liu,Jingui Li
A novel amine-free temperature-responsive biphasic DES (BLevA-OA) was developed. • Reversible phase transition integrated polyphenol extraction and separation. • GSWOA-SVR machine learning accurately optimized the extraction process. • MD simulations revealed molecular mechanisms of extraction and phase switching. • TRDES system outperformed traditional ethanol in extraction efficiency. The sustainable recovery of bioactive phytochemicals is frequently hindered by the toxicity and separation bottlenecks of conventional volatile organic solvents. Herein, a novel amine-free temperature-responsive biphasic deep eutectic solvent (TRDES) system, composed of betaine, levulinic acid, and octanoic acid (BLevA-OA), was constructed for the integrated extraction and separation of polyphenols from Moringa oleifera Lam. ( M. oleifera ) leaves. This system exhibits reversible thermomorphic behavior, transitioning from a homogeneous single phase at 60 °C to maximize mass transfer, to a biphasic state at 25 °C for spontaneous phase-selective separation. To address the nonlinear complexity of the extraction process, a hybrid Gaussian Whale Optimization Algorithm-Support Vector Regression (GSWOA-SVR) model was developed, which precisely optimized the parameters to achieve a maximum total polyphenol content of 39.8 mg GAE/g DW, which is 1.7 times that of conventional ethanol extraction. Furthermore, multiscale computational analyses combining COSMO-RS and Molecular Dynamics (MD) simulations elucidated the microscopic mechanism: the superior extraction efficiency stems from a cooperative network of specific hydrogen bonding and hydrophobic interactions, while the temperature-triggered phase transition is driven by the kinetic modulation of component miscibility and diffusion. This work establishes a sustainable, closed loop strategy for the high efficiency recovery of polyphenols from M. oleifera leaves, offering a theoretical and practical foundation for the industrial application of smart green solvents in the circular bio-economy. Download: Download high-res image (236KB) Download: Download full-size image
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MICROCHEMICAL JOURNAL
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2026
·
IF 5.1
Sai-Nan Qin,Yi-Xue Zhang,Yi-Jie Cao,Ting Wan,Yi-Yang Zhou,Shan-Shan Su,Yu-Qiong Guo,Jian-Jun Sun,Kalle Salminen
Cisplatin (DDP), as a broad-spectrum anticancer drug, requires dynamic monitoring of its blood concentration and intervention in its dosage administration to balance efficacy and safety due to significant individual differences in its metabolism, side effects, and resistance in different patients. However, rapid and robust methods for monitoring and quantifying DDP in complex biological fluids are still lacking. This work presents an electrochemical aptamer-based ( E -AB) sensor for rapid measurement of DDP concentration in blood. In which capture probe aptamers were designed constructed by visualizing and analyzing their binding pockets, followed by molecular docking and circular dichroism to screen the aptamer Apt25, which has a small binding energy and a large conformational change, for use in the construction of the sensor. In the presence of DDP, the aptamer undergoes a conformational change at the electrode interface, altering the electron transfer rate of the electroactive label to detect DDP concentration variations. After systematically using electrochemical techniques to optimize the sensor testing conditions, the sensor can directly analyze and detect DDP in urine and blood with a measurement range of 0.001–2 mM and a detection limit of 1 μM, which satisfies the needs of clinical testing. The spiked recoveries in different biological samples were 80.8–110% and 79–115.2%, respectively. In addition, the sensor has sufficient selectivity and stability, which is expected to enable real-time monitoring of DDP concentration for dosage adjustment and improvement of drug bioavailability at the clinical site.
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ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Xiao-Dong Cheng,Zheng Zhang,Yun-Ping Li
Green chromatography techniques using low-toxic mobile phase are getting increasingly attention in recent years. The core is developing stationary phases that possess adequate retention and separation under the mobile phase of high content water. Using thiol-ene click chemistry, an undecylenic acid-bonded silica stationary phase (UAS) was prepared in a facile manner. Elemental analysis (EA), solid-state 13 C NMR spectroscopy and Fourier transform infrared spectrometry (FT-IR) confirmed the successful preparation of UAS . The synthesized UAS was employed for per aqueous liquid chromatography (PALC), which uses little organic solvent during separation. Due to the hydrophilic carboxy, thioether group and hydrophobic alkyl chains of the UAS, various categories of compounds (including nucleobases , nucleosides , organic acids and basic compounds) with different properties can achieve enhanced separation under the mobile phase of high content water compared with commercial C18 and silica stationary phases. Overall, our present UAS stationary phase shows excellent separation ability toward highly polar compounds and meets the requirements of green chromatography.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 11
·1-10
·
IF 5.479
Zhang Yi,Jin Zhenkai,Zeng Qingsong,Huang Yanmei,Gu Hang,He Jiahua,Liu Yangyang,Chen Shili,Sun Hui,Lai Jiaping
A test strip has been developed for the rapid detection of the illegal additive ethyl anthranilate (EA) in wine. The detection scheme is based on a combination of photonic crystal based detection and molecular imprinting based recognition. The resulting molecularly imprinted photonic crystal (MIPC) undergoes a gradual color change from green to yellow to red upon binding of EA. A semi-quantitative colorimetric card can be used to estimate the content of EA, either visually or by making use of an optical fiber spectrometer. A linear relationship was found between the Bragg diffraction peak shift and the concentration of EA in the range from 0.1 mM to 10 mM. The detection limit is 10 μM. The test has been successfully used to screening for the presence of EA in grape wine. The test strip is selective, and can be re-used after re-activation.
Schematic representation of the fabrication and application of the molecularly imprinted photonic crystal (MIPC) based test trip. The resulting MIPC undergoes a gradual color change from green to yellow to red upon binding of the illegal wine additive ethyl anthranilate (EA).
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KEY ENGINEERING MATERIALS
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2019
·
IF 0
Shi Li Chen,Hui Sun,Zhen Jian Huang,Si Yang Fang,Jia Hua He,Yang Yang Liu,Jia Ping Lai
A “smart” molecular imprinted photonic crystal (MIPC) sensor has been developed based on the combination of photonic crystal (PC) and molecular imprinting technique (MIT). It can give rise to readable optical signals and color changes upon sensing benzocaine with detection limit of 16.5 μg/ml. In fish sample detection, the developed MIPC sensor shows satisfied result accuracy with HPLC. With the advantage of satisfactory specificity, rapid responsiveness and excellent recoverability, this MIPC material shows great potential towards on-site screening and visual detection of trace benzocaine in real sample.
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RSC Advances
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2019
·Vol. 9
·期 29
·16831-16838
·
IF 3.049
Shili Chen,Hui Sun,Zhenjian Huang,Zhenkai Jin,Siyang Fang,Jiahua He,Yangyang Liu,Yi Zhang,Jiaping Lai
A novel smart sensor for the rapid and label-free detection of benzocaine has been developed based on the combination of photonic crystal (PC) and molecular imprinting polymer (MIP) techniques. A molecularly imprinted photonic crystal (MIPC) hydrogel film was prepared via a non-covalent, self-assembly approach with a PC mould. With a highly ordered inverse opal structure, the resulting benzocaine MIPC exhibited high sensitivity, smart specificity, quick response times and good regeneration abilities. It can give rise to a readable optical signal and color change upon binding with benzocaine, with a detection limit of 16.5 μg mL−1. The sensor has been successfully used to visually estimate benzocaine concentrations in fish samples. In comparison with HPLC, the developed MIPC sensor has shown satisfactory accuracy in terms of results. It has great potential for on-site screening and the visual detection of trace benzocaine in real samples.
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Nanoscale
·
2016
·Vol. 8
·期 5
·2999-3007
·
IF 7.76
Min Lin,Hong Yan Zou,Tong Yang,Ze Xi Liu,Hui Liu,Cheng Zhi Huang
The inner filter effect (IFE), which results from the absorption of the excitation or emission light by absorbers, has been employed as an alternative approach in sensing systems due to its flexibility and simplicity. In this work, highly photoluminescent carbon nanodots (CDs), which were simply prepared through a new one-step microwave synthesis route, were loaded in electrospun nanofibers, and the obtained nanofibers were then successfully applied to develop a fluorescent IFE-based visual sensor for tetracycline hydrochloride (Tc) sensing in milk. This developed visual sensor has high selectivity owing to the requirements of the spectral overlap between the CDs and Tc, showing high promise in sensing chemistry with an efficient response and economic effect.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Yan Qiaofang,Huang Fei,Wang Chuyang,Zhan Min,Zhang Qiaoxuan,Huang Di,Yan Jun,Lin Haibiao,Huang Xianzhang,Han Liqiao
Digoxin, a cardiac glycoside, is widely used in the treatment of cardiovascular diseases. Due to its narrow therapeutic range, precise monitoring of its blood concentration is essential. A reference measurement procedure (RMP) is pivotal for ensuring result accuracy and comparability. The RMP for serum digoxin by ID-LC-MS/MS was optimized with sample pre-treatment and detection processes, and the bracketing calibration method was used, which facilitates more accurate measurement, especially for extreme concentrations. The performance of this optimized RMP was thoroughly evaluated. The limit of detection (LoD) was 0.05 ng/mL (0.06 nmol/L) and the lowest limit of quantification (LLoQ) was 0.10 ng/mL (0.13 nmol/L). The intra- and inter-assay imprecisions were 2.24%, 2.51%, 1.40% and 1.72%, 1.65%, 0.97% at 0.5, 2.0, 5.0 ng/mL, respectively. Recoveries were 99.63 to 101.42% and the linear response ranged from 0.1 to 10.0 ng/mL. The relative bias was 0.41% and 2.00% of our results compared with the median of all participating reference laboratories for IFCC-RELA (External Quality Assessment Scheme for Reference Laboratories in Laboratory Medicine) 2023A and 2023B. The uncertainty, calibration and measurement capability (CMC) of this method were also evaluated. The optimized RMP was applied in the Trueness Verification Plan of Southern China, which indicates significant differences among clinical systems, highlighting the need for standardization efforts. In addition, two commonly used clinical systems which employed immunoassay methods were compared with this optimized RMP, and 26 individual serum samples were analyzed. The good correlations indicate the feasibility of standardization for serum digoxin. The optimized RMP serves as an accurate reference baseline for routine methods, aiming to enhance the accuracy and precision of measurements in clinical laboratories. Graphical Abstract
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Yu Zhou,Zhijie Wang,Penghu Niu,Zhangqiang Chen,Zhonghua Li,Mingru Su,Yunjian Liu
Silicon (Si) is a potential fast-charging anode material for lithium-ion batteries (LIBs) due to its high energy density and suitable lithium insertion potential. However, the slow kinetics and significant volume changes during lithiation/delithiation hinder its practical application. High-entropy alloying of silicon enhances electronic conductivity and mitigates volume expansion, leading to improved rate performance. Nevertheless, the synergistic effects of high-entropy alloying and crystal structure on silicon-based anodes remain underexplored. Herein, a ternary doping alloy (Si-FeTiP) anode material with an amorphous structure was prepared via high-energy ball milling. The uniformly distributed microcrystalline phases of FeSi 2 and TiP enhanced the electronic conductivity and structural stability of the Si anode. The local disordered structure of the amorphous silicon phase mitigates lithiation-induced stress, while the isotropic nature of the amorphous structure facilitates excellent Li + diffusion kinetics in the Si-FeTiP composite. As a result, the Si-FeTiP anode exhibits an excellent rate capability of 658 mAh g −1 at 10 A g −1 and a capacity retention of 80.3 % after 500 cycles at 2 A g −1 . This study enhances our understanding of how crystal structure influences ion transport and electrochemical performance. Furthermore, it provides valuable insights for the design of multivariate fast-charging silicon-based anode materials.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Yingying Wang,Linping Ma,Shaogui Wu
Food safety concerns regarding Sudan Red, a carcinogenic dye, necessitate effective detection methods. This study introduces S, P co-doped blue-emitting carbon dots (BSP-CDs), synthesized via a one-pot hydrothermal method using asparagine as the carbon source. BSP-CDs exhibit excellent optical properties, environmental stability, and selective fluorescence quenching due to the inner filter effect. These properties were utilized to develop a sensitive and rapid “on-off” fluorescence sensing system for Sudan Red detection. The sensor demonstrated a linear detection range of 50–175 μM and a detection limit of 2.11 μM, with high accuracy and recovery rates in real-world water samples. This work offers a green, economical, and highly reproducible approach for Sudan Red monitoring, with significant implications for food safety and environmental protection.
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PHYTOCHEMICAL ANALYSIS
·
2017
·Vol. 28
·期 4
·332-343
·
IF 2.292
Qing Wang,Yao Long,Lin Yao,Mao Ye,Li Xu
Introduction Traditional methods for isolating and purifying quaternary ammonium alkaloids from Coptis chinensis do not discriminate the target alkaloids from co-extractives. Mixed-mode analytical chromatography has the potential to improve the separation of analytes in more complex extracts and, when used in a solid-phase extraction mode, improve the purity of isolated compounds. Objective To examine the high-performance liquid chromatographic separation capabilities of a mixed-mode, silica-based adsorbent and its application to the purification of quaternary ammonium alkaloids from Coptis chinensis based on solid-phase extraction. Methodology The C18-COOH silica was prepared via “thiol-ene” click chemistry. Its chromatographic performance was firstly investigated. It was employed as the solid-phase extraction sorbent to purify quaternary ammonium alkaloids. Results Hydrophobic, attractive/repulsive electrostatic and ion-exchange interactions were demonstrated to be the possible retention mechanisms of a C18-COOH silica stationary phase, which could separate analytes of various properties. In addition, to purify quaternary ammonium alkaloids from Coptis chinensis, the solid-phase extraction approach based on the C18-COOH silica provided a slightly higher purification efficiency (6.7%) than an alkaloid-salt precipitation protocol (5.3%). The method had satisfactory reproducibility, re-using the same solid-phase extraction column three times, with relative standard deviations ranging from 1.99% to 8.02% for the six target alkaloids. Conclusion A multi-functionalised silica was synthesised via “click chemistry”. As the high-performance liquid chromatographic stationary phase, the C18-COOH silica could be operated in trimodal reversed-phase/weak cation exchange/hydrophilic interaction mode. The C18-COOH silica also exhibited potential as solid-phase extraction sorbent in the purification of quaternary ammonium alkaloids from complex matrices. Copyright © 2017 John Wiley & Sons, Ltd.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yu Xie,Jiawen Yin,Fei Deng,Lingfeng Xu,Kalle Salminen,Limin Liu
It is important to develop clinical point-of-care testing (POCT) methods to avoid side effects and even fatalities due to anesthetic drug overdose. Due to their inherent advantages (e.g. ease of modification, thermal stability, and low cost), electrochemical aptamer-based (EAB) sensors are promising alternatives to conventional analytical methods. However, most of the current methods require some complex modification steps for the preparation of electrodes or signal probes as well as the indirect measurement of signals, and the application of these methods as POCT devices remains a challenge. In this work, a sandwich-type EAB sensor was developed for the direct determination of procaine in biofluids. The original aptamer was split into two segments, with one fragment modified with thiol and the other modified with a redox label. The detection process was rapid as conformational changes occur almost instantaneously when the aptamer binds to the target. Moreover, the binding properties of the aptamer towards the target were characterized by circular dichroism spectroscopy, molecular docking simulations and apparent electron transfer rates test. The simple, cost-effective and reagentless sandwich-type EAB sensor achieved nanomolar detection of procaine with a limit of detection (LOD) of 1.9 nM, 5.4 nM and 3.3 nM in buffer, 5 % urine and 2.5 % serum, respectively. This work is expected to play a role in the field of clinical drug analysis.
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