Analytical Methods
·
2018
·Vol. 10
·期 18
·2102-2107
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IF 2.073
Yuhao Xiong,Fang Li,Jingwen Wang,Ailing Huang,Mei Wu,Zhi Zhang,Dongjian Zhu,Wei Xie,Zhenhua Duan,Linjing Su
The determination of selenium (Se) has garnered increasing interest because of its importance to life as a nutrient element as well as its toxicological effects. Herein, a simple multimodal colorimetric method was developed for the detection of selenium. Firstly, thiol cotton is used as the functional material for simple solid-phase extraction separation of Se(IV). Then, the enriched Se(IV) oxidizes I− ions into I2 species, leading to the formation of linear triiodide ions that intercalate into the coil of starch molecules, producing a blue color. Based on the changes in the intensity of the blue color, multimodal detection methods using spectrophotometric detection, smartphone app-based colorimetric detection, and naked-eye colorimetric detection were developed for selenium detection. The spectrophotometric detection mode exhibited a linear response from 0.01 to 8.0 μg mL−1 with a detection limit of 0.0047 μg mL−1; the smartphone app-based colorimetric detection mode was linear from 1.0 to 6.0 μg mL−1 with a detection limit of 0.05 μg mL−1; and selenium levels of 0.1 μg mL−1 could be clearly distinguished by the naked eye. The colorimetric detection method was demonstrated to be applicable for the detection of selenium in real water and vegetable samples, giving recoveries of 94.1 to 108.4%.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
JOURNAL OF FLUORESCENCE
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2025
·
IF 3.1
Zu Panru,Yu Yanchao,Li Bolin,You Jun,Wu Wenju,Wu Mianyuan
A novel fluorescent probe XL derived from dicyanoisophorone was designed and synthesized, achieving successful sequential detection of Cd 2+ and GSH. As probe XL interacted with Cd 2+ , the emission wavelength redshifted from 565 nm to 650 nm, accompanied by enhanced red fluorescence, which realising a fluorescence turn-on response in the near-infrared region. A large Stokes shift (121 nm) combined with low detection limit (0.484 µmol/L) were key advantages of the detection process. However, due to high affinity of GSH for Cd 2+ , as addition of GSH to complex XL-Cd 2+ , fluorescence response in NIR region was switched off and emission peak was blue-shifted back to 565 nm. Detection limit for GSH was calculated as 0.784 µmol/L, a value significantly lower than its normal intracellular concentration, and the assay process with good sensitivity. Notably, probe XL showed an immediate response (3 s) to Cd 2+ and GSH, with a clear visual detection in natural light. In addition, XL could be used to detect Cd 2+ and GSH in real samples and achieve linked applications with smartphones.
研究领域:
分析化学
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生物传感
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环境科学
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材料科学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 3
·1-12
·
IF 5.833
Yao Chixuan,Liu Qingrun,Zhao Ning,Liu Jing-Min,Fang Guozhen,Wang Shuo
A simple dual-colour fluorescent nanoprobe has been designed composed of blue and yellow emission carbon quantum dots (CQDs). This system is inexpensive and easy to operate and was successfully employed for on-site measurements based on a smartphone app. The designed nanoprobe exhibited increased selectivity for Cr(VI), leading to a double stable response of the two CQDs. The dual-emission nanoprobe showed blue-violet fluorescence upon UV irradiation, and the fluorescent emission peaks were located at 418 nm and 552 nm. The blue light emission of CQDs was quenched with increasing Cr(VI) concentration due to the inner filter effect, whereas the yellow light emission was enhanced due to the aggregation-induced emission effect. The different responses of the dual emissions to Cr(VI) resulted in a fluorescent colour variation, thus enabling facile macroscopic visualization. With a smartphone, the change in the fluorescence colour could be observed more apparently than that of a single fluorescence nanoprobe, and the response increased linearly so that the nanoprobe could be applied to instantaneous measurements. Furthermore, the dual-emission nanoprobe was successfully employed for analysing food and water samples. Accurate concentrations were obtained by constructing a calibration plot using a fluorescence spectrometer and a smartphone app; the recoveries were 81.6% to 107.7%, and the relative standard deviation was below 3.6%. Therefore, this smartphone-integrated dual-emission detection system is promising as a new portable method for the on-site measurement of Cr(VI) ions. Graphical abstract
* Y-CQDs: yellow emission carbon quantum dots. B-CQDs: blue emission carbon quantum dots. B/Y-CQDs: a mixture of B-CQDs and Y-CQDs.
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 12
·1-11
·
IF 5.3
Tan Suhui,Tan Mengyuan,Zhang Tao,Gao Lu,Li Yi,Xu Xuechao,Yang Zhen-quan
A multienzyme-like L-argininemodified CeO 2 (Arg-CeO 2 ) nanozyme was prepared for accurate total antioxidant capacity (TAC) determination of Ganoderma sichuanense ( G. sichuanense ). It exhibits oxidase-like and peroxidase-like activities catalyzing the generation of superoxide anion free radicals and hydroxyl radicals, accelerating 3, 3′, 5, 5′-tetramethylbenzidine (TMB) chromogenic reaction. Antioxidants can effectively eliminate the generated free radicals, inhibiting the TMB chromogenic reaction. Hence, TAC can be assessed by Arg-CeO 2 catalyzed TMB chromogenic reaction using ascorbic acid (AA) as a reference substance. TAC of G. sichuanense extracted using different solvents was determined, indicating the highest TAC observed in the G. sichuanense extracted by the mixture solvent (deionized water/ethanol/ethyl acetate = 1 : 1 : 1). A visual assessment platform based on Arg-CeO 2 hydrogel nanozyme utilizing a self-programmed smartphone app and a self-designed 3D printed device is established. TAC using AA as a reference substance (3.33 ~ 83.33 µM) can be detected with a detection limit as low as 0.58 µM. The platform can realize one-step quantitative determination of TAC in G. sichuanens e within 40 min. The proposed visual assessment platform presented exhibits promising application prospects in the field of TAC assessment in food. Graphical
研究领域:
食品科学
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生物化学
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材料科学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
·
2025
·
IF 2.5
Zongning Guo,Xuelin Huang,Zhiqiang Mai,Li Yang,Shaofeng Zheng,Jiaying Liao,Feng Gao,You Zhang,Zhe Jiao
The radioactive uranium element associated with rare earth minerals poses a significant threat to human health. Current detection of uranium relies on analysis in the lab, which cannot meet the demand for rapid on-site detection. We have developed a porphyrin-based covalent organic framework material, COF-MF, which exhibited specific fluorescent response to uranyl ion (UO22+). Based on the combination of the imino group of the hydrazone with the hexavalent uranium on the skeleton, it blocked intramolecular charge transfer, leading to fluorescence quenching, without interference from other coexisting metal cations. The fluorescence intensity of COF-MF at 680 nm gradually decreased with UO22+ concentrations ranging from 0.1 nM to 4.0 mm, and a linear relationship was observed in the concentration range from 0.1 nM to 10 nM. By integrating 3D printing technology, we developed a portable, rapid on-site detection device combined with smartphone-based app detection software. The response curve of UO22+ from 0.1 nM to 4.0 mm was studied, and the detection limit of the device for UO22+ was 0.05 nM, which was lower than the natural radioactivity limit value of non-ferrous metal mineral products. The analysis results of uranium in standard material (GWB04122) were consistent with ICP-MS. This study can be used for rapid on-site screening of uranium in imported ores by customs, providing important technical support for the quality control of imported ores.
研究领域:
材料科学
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环境科学
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放射化学
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DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 36
·3598-3604
·
IF 3.532
Wenxiu Huang,Lizhen Zhao,Ruidi Shen,Gongke Li,Liansheng Ling
A highly sensitive and selective RGB color analysis for the detection of formaldehyde (FA) was developed by using a DNA functionalized gold nanoparticle (AuNPs-DNA) probe. When complementary oligonucleotides (oligo 2 and oligo 3) and a silver ion (Ag+) were added to the AuNPs-DNA solution, triplex DNA was formed, resulting in the aggregation of AuNPs, and accompanied by a solution color change from red to purple. With the addition of formaldehyde, it reacted with Ag+, decreased the stability of triplex DNA between AuNPs–DNA, induced the dispersion of AuNPs, and the color of AuNPs recovered to red. Therefore, the formaldehyde concentration could be estimated with the RGB (red, green, blue) values of the AuNP solution by using a smartphone application (APP). The R value of the system was proportional to the concentration of formaldehyde within the range of 0.23–4.50 mg L−1, with a detection limit of 0.14 mg L−1. The method has been successfully applied to detect the residues of formaldehyde in vegetable samples and has the potential of the on-site determination of formaldehyde.
研究领域:
生物化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2024
·
IF 8.5
Man Luo,Ji Liu,Yating Zhang,Tao Wang,Xiaomei Ren,Lijuan Gui,Junyuan Zhao,Xuwei Zhang,Yunqing Tang,Ziting Zeng,Fengzhen Hou,Qifeng Zhong,Zhenwei Yuan,Hui Xu
Food freshness monitoring is an important component in ensuring food safety for consumers and the food industry. Therefore, there is an urgent need for a portable, low-cost, and efficient detection method to determine the freshness. In this study, polyvinyl alcohol (PVA) was used as polymer carrier to prepare electrospinning film containing curcumin (Cur) and gardenia blue (GB) as intelligent indicator label on food packaging for real-time nondestructive detection of freshness of shrimp. The detection limit of ammonia response is less than or equal to 20 ppm, and the detection time is about 1 min, indicating that it has a sensitive response effect. At the same time, a smartphone application that can identify amines in response to color changes has been developed, and consumers can understand freshness by scanning the label. This study demonstrates the huge potential of smart indicator labels for food freshness monitoring.
研究领域:
食品科学
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生物技术
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PubMed
高影响因子
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
·
2023
·Vol. 59
·期 6
·946-958
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IF 0.8
Zhong T.,Li J.,Zhang S.,Pan H.
Abstract Interleukin-6 (IL-6) is essential for regulating the immune system and initiating acute inflammatory responses. While normal levels of IL-6 are very low in healthy individuals, current detection methods rely primarily on sophisticated instrumentation in central laboratories. Here, we developed a novel fluorescent immunochromatographic test strip method based on CdSe@ZnS quantum dots for point-of-care testing of IL-6. The test strip takes only 20 min to test the sample. Four analysis methods were developed: a self-developed smartphone application, smartphone photographs analyzed with ImageJ, a self-constructed portable immunoanalyzer, and a commercial immunoanalyzer. The performance of these four methods was evaluated and compared. The commercial immunoanalyzer achieved the best limit of detection (LOD) at 2.65 pg/mL, followed by the portable immunoanalyzer (5.74 pg/mL) and ImageJ (8.25 pg/mL). These LODs were sensitive enough to quantify IL-6 in healthy human serum around the clinically relevant cut-off of 10 pg/mL. Despite a higher LOD of 50 pg/mL, customized smartphone application enabled rapid IL-6 quantification and screening, which is useful in resource-scarce areas and well suited for measuring IL-6 levels. The developed method provides a simple, rapid, and portable tool for diagnosing and monitoring IL-6-related diseases.
研究领域:
医学诊断
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生物化学
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纳米技术
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2025
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IF 8.2
Meixuan Li,Fengzhen Yang,Kaiyuan Jia,Qiang Zhang,Weichao Zheng,Hui Chen,Ming Liao,Jianhan Lin,Lei Wang
Bioaerosol infections containing pathogenic viruses and bacteria have resulted in significant economic losses and posed a serious threat to public health, as evidenced by outbreaks of coronavirus disease and avian influenza. Consequently, the sampling and screening of bioaerosols are crucial for the prevention of bioaerosol-borne diseases. In this study, an ultrasensitive biosensor based on zeolitic imidazolate framework-8-melamine foam (ZIF-8-MF) was innovatively developed for the specific capture and in situ detection of bioaerosols. The bacterial bioaerosols were collected by a wet cyclone into phosphate-buffered saline (PBS) buffer at a high collection rate, achieving a satisfactory collection efficiency of ∼80% within 10 min. The target bacteria collected in the PBS buffer were specifically captured and effectively concentrated using immune ZIF-8-MF. The gold@platinum nanozymes (GPNs) were employed to specifically label the captured target bacteria and efficiently amplify the biological signal. And the resulting colorimetric signal was analyzed via a self-developed smartphone application (App). This biosensor demonstrated the capability to detect bioaerosols containing Salmonella typhimurium in the range of 1.6 × 102–1.6 × 105 CFU/m3 within 1.5 h, with a detection limit as low as 100 CFU/m3. Compared with other bioaerosol detection methods, the biosensor offered advantages such as high collection rate, specific capture, efficient concentration, and in situ detection, positioning it as a highly promising and practical tool for the monitoring of bioaerosols containing diverse pathogenic bacteria.
研究领域:
环境生物学
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医学检测
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材料科学
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公共卫生
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
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IF 4.3
Yanmei Lu,Jiayue Liu,Wenjun Dong,Caizhao Cheng,Zhiyong Guo,Sui Wang,Yufang Hu,Zhihe Qing
Unsafe food additives pose a significant threat to global health, especially in developing countries. Many existing methods rely on clean laboratories, complicated optics equipment, trained personnel and lengthy detection time, which are not suitable for onsite food safety inspections in emergency situations, peculiarly in impoverished areas. In this paper, a fast and visual onsite method is designed for the detection and quantification of additives in food safety by engineering a nanohybrid (MoS 2 /SDBS/Cu-CuFe 2 O 4 )-based catalysis. Interestingly, the nanohybrid presents peroxidase-like mimetic activity toward the substrate containing 3,3′,5,5′-tetramethylbenzidine (TMB) and hydrogen peroxide (H 2 O 2 ), which are then integrated simply into a detection kit. The blue oxidated TMB in this kit can be converted completely to colorless by some bio-molecule additives in commercial food, such as glutathione (GSH), cysteine (Cys), and ascorbic acid (AA). Remarkably, this process takes just less than 2 min and the detection limits are 2.8 nM, 5.5 nM and 47 nM, respectively. These results show excellent repeatability with a statistical analysis with (*P < 0.05) over 30 tests. Next, the images of the color changes can be captured clearly using a smartphone by red–green–blue (RGB) channels, which provides an opportunity for the development of field-operation device. Additionally, our approach is applied to some targets-indicative foods, showing a recovery range between 95.8 % and 104.2 %, offering an attractive and promising pathway for future practical food safety inspection applications. More importantly, this method can easily be extended to the detection of reducing substances in other analytical fields.
研究领域:
食品安全
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纳米技术
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分析化学
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DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2022
·
IF 7.968
Xueyan Liu,Zhuo Chen,Rongsheng Gao,Chengyou Kan,Jianhong Xu
Technological advances provide the possibility to develop a quantitative detection platform based on the smartphone as a detector. In this work, we reported a portable, simplified and on-site colorimetric method for Fe 3+ sensing owing to the rhodamine-grafted paper and smartphone mini application. According to a rhodamine-based probe RhBUEA, the rhodamine-grafted cellulose filter paper (Paper- g -RhBUEA) was prepared via ARGET ATRP as a fluorescent and colorimetric sensor for Fe 3+ , and their physicochemical properties were characterized by XPS , ATR-FTIR, SEM, EDS and spectrophotometer. Simultaneously, the influence of the RhBUEA concentration on the grafting rate and fluorescence intensity was studied. Based on the recipe of 0.056 mol/L RhBUEA, Paper- g -RhBUEA showed excellent selectivity and sensitivity for Fe 3+ monitoring in the study of fluorescent and colorimetric properties. The detection limit of the fluorescent and colorimetric recognition is 0.19 μM and 1.4 μM, respectively. To increase the portability of Fe 3+ sensing, a smartphone WeChat mini application named “Colorimetric detector” was developed. The colorimetric detection platform integrating the Paper- g -RhBUEA as a probe, and the smartphone app as a processor permitted convenient and on-site colorimetric quantitative detection of Fe 3+ in environmental water.
研究领域:
分析化学
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环境科学
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生物医学工程
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DOI
高影响因子
Food Bioscience
·
2025
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IF 5.9
Wenhui Wang,Heera Jayan,Usman Majeed,Xiaobo Zou,Qin Hu,Zhiming Guo
The misuse of additives is a major problem for global food safety, and of particular concern is Auramine O (AO). Although the analysis of AO by fluorescent carbon dots (CDs) has been extensively explored, the development of effective sensitivity enhancement and portable detection solutions based on CDs remains a significant challenge. Herein, we developed a dual-signal ratiometric fluorescent probe (RFP) integrated with fluorescent nanopaper and smartphone application, which has been used to achieve portable visualization and quantitative detection of AO in food and drug samples. The specific response analysis model (RFP) for AO detection was constructed using fluorescence resonance energy transfer and electrostatic attraction between B-CDs and O-CDs. Within a concentration range of 0.0–10.0 μM, achieving a detection limit as low as 12.3 nM. Furthermore, a laboratory device that incorporates portable fluorescent nanopaper into a smartphone was developed to record the color information response ratio (R/B) in combination with the ColorColl application, enabling visual quantitative measurement of AO. This is powerful construction of RFP, which avoids time-consuming synthesis and complex modification. It directly demonstrates the superiority of proportional fluorescence detection over single wavelength detection. This work provides a referable perspective for preliminary monitoring and rapid on-site detection of harmful factors such as AO in food and drug samples.
研究领域:
食品安全
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分析化学
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生物传感
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）