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CHEMICAL PHYSICS LETTERS · 2019 · IF 1.901

A fraction of NADH in solution is “dark”: Implications for metabolic sensing via fluorescence lifetime

Simin Cao,Zhongneng Zhou,Haoyang Li,Menghui Jia,Yangyi Liu,Mengyu Wang,Mengjie Zhang,Sanjun Zhang,Jinquan Chen,Jianhua Xu,Jay R. Knutson

The metabolic cofactor and energy carrier NADH (nicotinamide adenine dinucleotide, reduced) has fluorescence yield and lifetime that depends strongly on conformation, a fact that has enabled metabolic monitoring of cells via FLIM (Fluorescence Lifetime Microscopy). Using femtosecond fluorescence upconversion, we show that this molecule in solution participates in ultrafast self-quenching along with both bulk solvent relaxation and spectral relaxation on 1.4 and 26 ps timescales. This, in effect, means up to a third of NADH is effectively “dark” for FLIM in the 400–500 nm observation window commonly employed. Methods to compensate for, avoid or measure dark species corrections are outlined.

CAS / MDL: 606-68-8

研究领域: 生物物理学 · 细胞生物学 · 代谢组学

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ACS Sustainable Chemistry & Engineering · 2023 · IF 8.4

Mechanistic Insights into the Selective Oxidation of 5-Hydroxymethylfurfural over Au/TiO2 Catalysts under Photoexcited and Dark States

Wenqing Yu,Ying Wang,Hanwen Zhang,Qiao Zhang,Shengyang Wang,Zhiting Liu,Guangxing Yang,Feng Peng

The 5-hydroxymethylfurfural oxidation reaction (HMFOR) is regarded as a promising approach to produce biomass-derived highly valuable chemical products. 5-Hydroxymethyl-2-furancarboxylic acid (HMFCA) as a typical product via selective oxidation of the aldehyde group in HMF was obtained on Au/anatase and Au/rutile catalysts under photoexcited and dark states. The results show that the HMF oxidation reactivities of Au/rutile and Au/anatase were improved under visible light irradiation compared with those under dark conditions, and the increase of the activity is more obvious as the reaction temperature decreases. The kinetic studies showed that visible light irradiation reduced the energy barrier of the HMF oxidation reaction and promoted the reaction activities. The radical capture and EPR experiments were further used to explore the mechanisms of the HMF oxidation reaction under both conditions over Au/TiO2 catalysts. The evidence indicated that the main active species were 1O2 and trace amounts of •O2– under dark conditions, while the main active species were •O2–, 1O2, and h+ under visible light irradiation conditions for both Au/rutile and Au/anatase catalysts.

CAS / MDL: 13463-67-7 1317-80-2

研究领域: 催化化学 · 生物质转化 · 材料科学

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A fraction of NADH in solution is “dark”: Implications for metabolic sensing via fluorescence lifetime

Mechanistic Insights into the Selective Oxidation of 5-Hydroxymethylfurfural over Au/TiO2 Catalysts under Photoexcited and Dark States