CARBOHYDRATE POLYMERS
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2021
·
IF 9.381
Min Wu,Xiaoxia Chen,Jiatong Xu,Hongbin Zhang
Physical gels from natural polysaccharides present the advantage of no toxic cross-linking agents and no chemical modification during preparation. Herein, novel physical gels, transparent organogels and opaque hydrogels from the microorganism-derived (1,3)- β -D-glucan of curdlan were prepared in dimethyl sulfoxide (DMSO) using the freeze-thaw technique, followed by a solvent-exchange strategy with water. The mechanical and structural properties of these gels were investigated by rheology , scanning electron microscopy, attenuated total reflection infrared spectroscopy , wide-angle X-ray diffraction and small-angle X-ray scattering. Gelation mechanisms and intermolecular interaction models have also been proposed. The good solvent DMSO serves as both a crosslinker and a pore-foaming agent in organogels. The reversible macromolecular conformation changes and phase separation of curdlan endow the gels with reversible transparency, volume change and tunable mechanical strength. The new design strategy of facile preparation and performance tuning provides a platform for developing new organogels and sterile hydrogels of curdlan.
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2018
·
IF 5.158
Rong Tang,Jie Hao,Ruijie Zong,Fangxia Wu,Yangyang Zeng,Zhenqing Zhang
In this study, the TEMPO-mediated (TEMPO/NaBr/NaClO) oxidation pattern of curdlan was investigated through comprehensively structural analysis of the corresponding oxidized products. During the structural analysis, infrared spectroscopy (IR), nuclear magnetic resonance (NMR) spectroscopy, gel permeation chromatography tandem multiple angle laser scattering (GPC-MALS) and ultra-high performance liquid chromatography tandem quadrupole time of flight mass spectrometry (UHPLC-Q/TOF-MS) were applied. As a result, the homogenous β1-3 polyglucuronic acids (MW, 49.8, 29.8 and 7.0 kDa) were obtained with proper amount of oxidant (5.36 mmol NaClO) at various temperatures (4, 25, 50 °C), respectively. Compared to the oxidation of 1–4 linked glucan (starch and cellulose) with TEMPO-mediated system at same reaction conditions, higher degree of specific oxidation and less degradation were observed in that of 1–3 linked curdlan. The glycosylation at position 3 could stabilize the sugar ring, which inactivates the non-specific oxidation related hydroxyl groups on the sugar ring. Thus, the TEMPO-mediated system has higher selectivity to oxidize the primary hydroxyl groups of 1–3 linked curdlan and form polyglucuronic acid than those observed in the oxidation of starch and cellulose. In addition, same as those observed in previous work about starch, higher the temperature was used in the oxidation with TEMPO system, higher the activity of oxidant (NaClO solution) was, more non-specific oxidation occurred, and more the degradation were observed.
研究领域:
生物化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Xinyi Li,Sha Liu,Liya Niu,Shuai Hu,Chao Sun,Zicong Zeng,Jianhui Xiao
This study investigated the effect of different curdlan (CUD) concentrations on the frozen sweet potato starch vermicelli (FSPSV) during 10 freeze-thaw (FT) cycles. The results showed that CUD efficiently mitigated the quality deterioration of FSPSV at all levels during FT cycles, with a more pronounced effect observed at higher concentrations. Notably, the 1.20% CUD sample at FT-10 still exhibited superior textural properties compared to the control at FT-0. The SEM and FTIR results illustrated that CUD displayed intense interactions with SPS to form a denser gel network structure and thus improved the freeze-thaw stability of FSPSV by decreasing the freezable water content, total relaxation time, and porosity of FSPSV by 16%, 47%, and 66%, respectively. In addition, analysis of the CUD-SPS-water ternary system further revealed the contribution of CUD in establishing a more robust gel system to ameliorate the adverse effects of ice crystals, with the notable improvement in the average particle size and peak viscosity of SPS, reducing the Tan σ value and phase transition time of SPS gel from 0.58 and 181.67 min to 0.29 and 142.67 min, respectively. Our results suggested that CUD may provide new insights into its application in frozen starch-based food.
研究领域:
食品科学
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生物化学
✓ 已验证
DOI
高影响因子
Pharmaceutics
·
2021
·Vol. 13
·期 9
·1518
·
IF 6.321
Mengting Duan,Wei Fan,Bing Fan
Mesoporous calcium-silicate nanoparticles (MCSNs) are excellent biomaterials for controlled drug delivery and mineralization induction. In this study, MCSNs were loaded with low-dose silver ion (Ag+) and Triton X-100 (TX-100) as the M-AgTX to achieve both enhanced antibacterial properties and low cytotoxicity for dentin disinfection. The physicochemical property, biocompatibility, infiltration ability into dentinal tubules, anti-bacterial ability against both planktonicEnterococcusfaecalis(E. faecalis) and its biofilm on dentin, effects on dentin microhardness and in vitro mineralization property were systematically investigated. Results confirmed that the MCSNs and M-AgTX nanoparticles showed typical morphology of mesoporous materials and exhibited sustained release of chemicals with an alkaline pH value over time. M-AgTX also exhibited excellent biocompatibility on MC3T3-E1 cells and could eliminate 100% planktonicE. faecalisafter 48-h treatment. On dentin slices, it could enter dentinal tubules by ultrasonic activation and inhibit the growth ofE. faecalison dentin. M-AgTX could completely inactive 28-dayE. faecalisbiofilm. TEM confirmed the destruction of cell membrane integrity and Ag+infiltration into bacteria by M-AgTX. Besides, dentin slices medicated with M-AgTX nanoparticles displayed an increased microhardness. After being immersed in SBF for 7 days, apatite crystals could be observed on the surface of the material tablets. M-AgTX could be developed into a new multifunctional intra-canal medication or bone defect filling material for infected bone defects due to its sustained release profile, low cytotoxicity, infiltration ability, enhanced anti-bacterial and mineralization features.Keywords:mesoporous;nanoparticle;Enterococcus faecalis;silver;Triton X-100;root canal disinfection
研究领域:
牙科医学
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生物材料学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Microorganisms
·
2022
·Vol. 10
·期 1
·124
·
IF 4.926
Xinling He,Siqi Jin,Wei Fan,Bing Fan
The prevention and treatment of oral diseases is more difficult in diabetic patients with poorly controlled blood glucose levels. This study aims to explore an effective, low-cytotoxicity medication for root canal treatment in diabetic patients. The antibacterial effect of the combination of Triton X-100 (TX-100) and metformin (Met) onEnterococcus faecalis(E. faecalis) was evaluated by determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration required to kill 99% bacteria (MBC99) and by conducting dynamic time-killing assays. While the antibiofilm activity was measured by crystal violet (CV) assay, field emission scanning electron microscope (FE-SEM), confocal laser scanning microscope (CLSM) and colony-forming unit (CFU) counting assays. The expression of relative genes was evaluated by real-time quantitative polymerase chain reaction (RT-qPCR), and the cytotoxicity of the new combination on MC3T3-E1 cell was also tested. Results showed that the antibacterial and antibiofilm activities of Met could be significantly enhanced by very low concentrations of TX-100 in both normal and high-glucose conditions, with a much lower cytotoxicity than 2% chlorhexidine (CHX). Thus, the TX-100 + Met combination may be developed as a promising and effective root canal disinfectant for patients with diabetes.Keywords:antimicrobial activity;dentinal tubules;Enterococcus faecalis;high-glucose condition;metformin;Triton X-100
研究领域:
牙科学
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微生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 20
·8129-8135
·
IF 1.938
Jinjiao Shi,Ligang Chen
A sensitive and simple method for high-performance liquid chromatography (HPLC) determination of Sudan dyes in lipstick samples after preconcentration by cloud point extraction (CPE) has been developed. The nonionic surfactant Triton X-114 was chosen as the extraction medium. Various experimental conditions were investigated to optimize the extraction and preconcentration process. Under optimum conditions, 3% Triton X-114 (v/v), ultrasonication for 20 min, pH 7, the satisfied recoveries of Sudan dyes were obtained. For the preconcentration of Sudan dyes by cloud point extraction, the solution was incubated in a thermostatic water bath at 60 °C for 10 min, and 22.5% sodium chloride (m/v) was added to the solution to facilitate the phase separation and increase the preconcentration factor. The limits of detection for the analytes are 0.11, 0.10, 0.07 and 0.08 μg g−1, respectively. The intra-day and inter-day precisions of four Sudan dyes are in the range of 4.6–6.4% and 4.4–6.7%, respectively. The recoveries of four Sudan dyes are 89.3%, 90.3%, 91.8% and 92.0%, respectively. The results demonstrate that this method is simple, rapid, sensitive and low cost. Moreover, it does not require the large consumption of organic solvents and is a non-toxic and eco-friendly method.
研究领域:
分析化学
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化妆品科学
·
环境化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）